Abstract:
:Three families of ligand-activated ion channels mediate synaptic communication between excitable cells in mammals. For pentameric channels related to nicotinic acetylcholine receptors and tetrameric channels such as glutamate receptors, the pore-forming and gate regions have been studied extensively. In contrast, little is known about the structure of trimeric P2X receptor channels, a family of channels that are activated by ATP and are important in neuronal signaling, pain transmission and inflammation. To identify the pore-forming and gate regions in P2X receptor channels, we introduced cysteine residues throughout the two transmembrane (TM) segments and studied their accessibility to thiol-reactive compounds and ions. Our results show that TM2 lines the central ion-conduction pore, TM1 is positioned peripheral to TM2 and the flow of ions is minimized in the closed state by a gate formed by the external region of TM2.
journal_name
Nat Neuroscijournal_title
Nature neuroscienceauthors
Li M,Chang TH,Silberberg SD,Swartz KJdoi
10.1038/nn.2151subject
Has Abstractpub_date
2008-08-01 00:00:00pages
883-7issue
8eissn
1097-6256issn
1546-1726pii
nn.2151journal_volume
11pub_type
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