Abstract：
:Genetically engineered mouse models are invaluable to investigators in nearly all areas of biomedical research. The use of genetically engineered mice has allowed researchers to explore fundamental functions of genes in a mammal that shares substantial similarities with human physiology and pathology. Genetically engineered mice are often used as animal models of human diseases that are vital tools in investigating disease development and in developing and testing novel therapies. Gene targeting in embryonic stem cells allows endogenous genes to be specifically altered. As knowledge regarding precise genetic abnormalities underlying a variety of dermatological conditions continues to emerge, the ability to introduce corresponding alterations in endogenous gene loci in mice, often at a single base pair level, has become essential for detailed studies of these genetic diseases. In this review, we provide examples of mouse models harboring modified endogenous gene(s), generated using the technique commonly referred to as the "knock-in" approach, to exemplify the important and sometimes superior role of this methodology in dermatological research.
journal_name
J Dermatol Scijournal_title
Journal of dermatological scienceauthors
Chen J,Roop DRdoi
10.1016/j.jdermsci.2008.03.012subject
Has Abstractpub_date
2008-10-01 00:00:00pages
1-12issue
1eissn
0923-1811issn
1873-569Xpii
S0923-1811(08)00119-9journal_volume
52pub_type
杂志文章,评审abstract：BACKGROUND:Netherton syndrome (NS, MIM 256500) is a potential live threatening autosomal-recessive skin disorder clinically characterized by the trias of congenital erythroderma, hair shaft anomalies and atopic diathesis. It is caused by mutations in the gene SPINK5 resulting in a deficiency of its processed protein na...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2010.12.004
更新日期：2011-03-01 00:00:00
abstract：:Histopathologically, the skin lesions of acute herpes zoster (AHZ) are characterized by epidermal necrotic vesicles with inflammation. Nitric oxide (NO) is generated from L-arginine by nitric oxide synthase (NOS), and immune inflammation involves the activation of NOS in both effector cells and target cells. NO can ca...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(02)00035-x
更新日期：2002-09-01 00:00:00
abstract：BACKGROUND:Pruritus and discomfort are often present in patients with xerosis and atopic dermatitis. Several studies suggest an important role of diet in skin pathophysiology. OBJECTIVE:This study evaluated the effect of dietary fatty acids in the skin physiology via an itch-related animal model with and without suppl...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2015.06.015
更新日期：2015-09-01 00:00:00
abstract：BACKGROUND:The effects of hyaluronic acid (HA) injection on tissue collagen anabolism are suggested to be related to the induction of mechanical stress, causing biochemical changes in skin physiology. OBJECTIVES:To ascertain the association between dermal mechanics modulated by a hyaluronic acid-based filler effect an...
journal_title：Journal of dermatological science
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jdermsci.2012.12.006
更新日期：2013-03-01 00:00:00
abstract：:Photochemotherapy using methoxsalen in combination with long-wave ultraviolet light (PUVA) is an essential modality in the treatment of various skin diseases. Major therapeutic regimens include oral, topical and water-delivery methods. An adequate regimen should be chosen regarding cases of disease, extent of involvem...
journal_title：Journal of dermatological science
pub_type： 杂志文章,评审
doi：10.1016/s0923-1811(98)00065-6
更新日期：1999-02-01 00:00:00
abstract：BACKGROUND:UV radiation is the major environmental factor related to development of cutaneous melanoma. Besides sun exposure and the influence of latitude, some host characteristics such as skin phototype and hair and eye color are also risk factors for melanoma. Polymorphisms in DNA repair genes could be good candidat...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2011.06.003
更新日期：2011-10-01 00:00:00
abstract：:Using fetal rat keratinizing epidermal cells (FRSK cells) we investigated the effects of retinoids on the cyclic AMP system. The beta-adrenergic adenylate cyclase response was significantly decreased by the treatment of cells with 1 x 10(-6) M Ro 10-1670. The effect was observed by 12 h and remained for at least 48 h....
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/0923-1811(93)90080-9
更新日期：1993-04-01 00:00:00
abstract：BACKGROUND:Our previous study showed that T cells in skin lesions of human atopic dermatitis (AD) had oligoclonal accumulation, indicating the involvement of antigen-specific immune reactions at those sites. Recently, NC/Nga mice, which develop skin lesions similar to AD, have been proposed as a model for that disease....
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2004.11.011
更新日期：2005-04-01 00:00:00
abstract：:Skin biopsies from four patients with similar clinical phenotypes of non-lethal junctional epidermolysis bullosa (JEB) were investigated by electron microscopy and immunohistochemistry. Electron microscopy revealed cleavage within the lamina lucida in each case, but in addition, there was considerable variability in t...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(96)00554-3
更新日期：1997-01-01 00:00:00
abstract：:Tumor necrosis factor-alpha (TNF-alpha) has been demonstrated to selectively decrease the production of type I and type III collagens in human dermal fibroblasts. The effects of the commonly used food antioxidants, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol, propyl gallate, supero...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/0923-1811(95)00463-7
更新日期：1996-04-01 00:00:00
abstract：:We developed a new method for evaluating clinical symptoms of psoriasis during the observation period, by adding a parameter of time (the number of days) to the psoriasis area and severity index (PASI). This method was named the evaluation for prognosis with averaged PASI (E-PAP). Using this method, we assessed 14 cas...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(97)00047-9
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND:Merkel cell carcinoma (MCC) is a neuroendocrine cancer of the skin postulated to originate through Merkel cell polyomavirus (MCPyV) oncogenesis and/or by mutations in molecules implicated in the regulation of cell growth and survival. Despite the fact that MCPvV is detected more broadly within the population...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2015.04.002
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND:cicatricial pemphigoid and bullous pemphigoid are characterized by circulating autoantibodies to the hemidesmosomal protein, BP180. Different clinical features between cicatricial pemphigoid and bullous pemphigoid appear to correlate with distinct target epitopes on BP180. Previous studies demonstrated that ...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(03)00035-5
更新日期：2003-06-01 00:00:00
abstract：:FK506, a new immunosuppressive agent, is effective in treating patients with psoriasis. A major feature of psoriasis vulgaris is the hyperproliferation of keratinocytes together with inflammation. To determine the effect of FK506 or cyclosporin A (CsA) on the keratinocyte cell cycle, flow cytometry and the growth fact...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/0923-1811(95)00480-7
更新日期：1996-09-01 00:00:00
abstract：BACKGROUND:Fascin is an actin-binding protein that regulates the rearrangement of cytoskeletal elements and their interactions with the cell membrane. Previous studies have indicated that fascin expression is enhanced in DC upon maturation and plays a critical role in T cell activation. Ultraviolet irradiation exerts i...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2005.05.004
更新日期：2005-12-01 00:00:00
abstract：:Autoantibodies to human carbonic anhydrase II (CAII) were screened by ELISA in 109 sera from Asian Japanese patients with systemic lupus erythematosus (SLE), primary Sjögren's syndrome (Sjs), progressive systemic sclerosis (PSS) and dermatomyositis (DM). Anti-CAII antibodies were positive in 24.1% of SLE, 20.0% of pri...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(99)00032-8
更新日期：1999-11-01 00:00:00
abstract：BACKGROUND:Reactive oxygen species (ROS)-induced mitochondrial damage aggravates oxidative stress and activates mitochondrial apoptosis pathway to mediate melanocyte death. However, the repair mechanisms underlying damaged mitochondria of melanocytes remain unclear. Accumulative evidence has revealed that the aryl hydr...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2019.09.001
更新日期：2019-10-01 00:00:00
abstract：:We previously reported a simple and easy-to-use device, Scratch Monitor for evaluation of nocturnal scratching. In the present study, the effect of an antihistamine (azelastine hydrochloride) on atopic dermatitis was investigated using this device. In 40 patients with atopic dermatitis, nocturnal scratching was measur...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(99)00048-1
更新日期：1999-12-01 00:00:00
abstract：BACKGROUND:Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH/D box family proteins and designated as a putative RNA helicase, which plays various roles in gene expression and cellular functions in response to a variety of RNA viruses. OBJECTIVE:The present study was designed to investigate the effects of ...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2006.11.003
更新日期：2007-02-01 00:00:00
abstract：:Angiogenesis is defined as the formation of new capillaries from pre-existing blood vessels. The process of angiogenesis is tightly regulated by a balance between pro- and anti-angiogenic factors. Vascular endothelial growth factor (VEGF) is a pro-angiogenic factor and several anti-VEGF therapies are used in the treat...
journal_title：Journal of dermatological science
pub_type： 杂志文章,评审
doi：10.1016/j.jdermsci.2010.03.023
更新日期：2010-06-01 00:00:00
abstract：BACKGROUND:As interest in skin beauty increases, the development of new skin whitening agents has attracted substantial attention; however, the action mechanism of the agents developed so far remains largely unknown. Tranexamic acid (TXA) is commonly being used to reduce melanin synthesis in patients with melasma and a...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2017.05.019
更新日期：2017-10-01 00:00:00
abstract：BACKGROUND:Skin is the outermost tissue of the human body, and works as a mechanical, chemical, and biological barrier. The epidermis is the uppermost layer of the skin, and keratinocytes constitute the majority of epidermal cells. Wounds are disruptions of skin integrity, and cause tremendous disadvantages to humans; ...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2016.10.008
更新日期：2017-02-01 00:00:00
abstract：BACKGROUND:Common DNA variants in IL12B, IL23R and IL23A have been associated with an increased susceptibility to psoriasis (Ps) and psoriatic arthritis (PsA). Metabolic comorbidities and cardiovascular risk factors have also been associated to both Ps and PsA. OBJECTIVE:To analyze the effect of single nucleotide poly...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2014.05.010
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND:Using JAK inhibitors to inhibit cytokine signaling is presumed to be a possible means of treating skin inflammatory disorders such as contact dermatitis. OBJECTIVE:To clarify the action site of JAK inhibitors in skin inflammatory disorders. METHODS:We analyzed the mechanism of action of the JAK inhibitor J...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2016.09.007
更新日期：2016-12-01 00:00:00
abstract：:Transdermal system(s) bearing captopril were developed using a low temperature casting method and aqueous based polymers viz., eudragit RL-100 and polyvinyl pyrrolidone (PVP). The developed system(s) were subjected to an in vitro characterization study. The results were compared with the transdermal systems of the sam...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/0923-1811(95)00403-f
更新日期：1995-11-01 00:00:00
abstract：:Psoriatic involved epidermis has been characterized by a defective beta-adrenergic adenylate cyclase response. It is also characterized by increased cholera toxin- and forskolin-induced cyclic AMP accumulations. Due to the fact that receptor signals are transduced to adenylate cyclase through guanine nucleotides bindi...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(97)00069-8
更新日期：1998-05-01 00:00:00
abstract：BACKGROUND:The analysis of prognostic factors in Japanese cases of cutaneous T-cell lymphoma (CTCL) is scarce. Clusterin is a ubiquitous 80kDa heterodimeric glycoprotein expressed on tumor cells of systemic and primary cutaneous anaplastic large cell lymphoma. The expression of clusterin in mycosis fungoides (MF) and S...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2013.04.020
更新日期：2013-09-01 00:00:00
abstract：BACKGROUND:Psoriasis is a chronic inflammatory dermatosis that has been associated with various cardiovascular and metabolic comorbidities, including myocardial infarction, stroke, and diabetes mellitus. Recently, there are studies reporting the association of psoriasis with renal diseases. OBJECTIVE:To evaluate the r...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2015.03.012
更新日期：2015-06-01 00:00:00
abstract：BACKGROUND:Ultraviolet (UV) A irradiation causes the degeneration of extracellular matrix in the skin dermis, mainly due to disrupted collagen homeostasis, resulting in the photo-aging of human skin. All-trans retinoic acid (ATRA) improves photo-aged human skin in vivo. OBJECTIVES:Although the effects of ATRA on colla...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2011.12.008
更新日期：2012-04-01 00:00:00
abstract：:Among occupational and environmental disorders, contact or photocontact dermatitis and an acneiform eruption are two major skin disorders. Photocontact dermatitis was historically caused by various halogenated salicylanilides, while the acne is induced by halogenated aromatic hydrocarbons and thus called chloracne. Th...
journal_title：Journal of dermatological science
pub_type： 杂志文章,评审
doi：10.1016/s0923-1811(03)00097-5
更新日期：2003-08-01 00:00:00
