Subpixel tracking for the analysis of outer hair cell movements.

Abstract:

CONCLUSION:Videomicroscopy with subpixel analysis is an excellent system for quantification of outer hair cell (OHC) movements. The resolution of a few nanometers is accurate enough to show induced differences of electromotility. OBJECTIVE:Electromotility of OHCs is a voltage-dependent process resulting from a membrane protein named prestin. Voltage sensitivity is conferred to prestin by intracellular anions. Reduction of these anions reduces electromotility. Videomicroscopy and subpixel tracking combine video-based analysis with a resolution of few nanometers. The aim of this study was to show the feasibility of a system for quantification of OHC movements. MATERIALS AND METHODS:Electromotility was investigated under normal and reduced intracellular chloride conditions. Cells were stimulated by the patch-clamp technique. Voltage steps were 500 ms long, ranging from -170 to +30 mV in 10 mV steps. RESULTS:As in previous studies our results show the following. The direction of OHC movement depends on the polarity of voltage steps, length changes are not equal for symmetrical voltage steps of opposite polarity, average shortening for a depolarizing step (-70 mV to +30 mV) is about 13 nm/mV. Hyperpolarization (-70 mV to -170 mV) on average evokes elongations of about 3 nm/mV. Half maximal chloride concentration reduces motility by 14%; half maximal electromotility is reached by a 94% reduction of chloride.

journal_name

Acta Otolaryngol

journal_title

Acta oto-laryngologica

authors

Canis M,Ortner M,Olzowy B,Jahn K,Strupp M,Hemmert W,Berghaus A,Suckfuell M

doi

10.1080/00016480701411502

subject

Has Abstract

pub_date

2008-03-01 00:00:00

pages

228-32

issue

3

eissn

0001-6489

issn

1651-2251

pii

788418452

journal_volume

128

pub_type

杂志文章
  • Intermediate filament proteins in the embryonic inner ear of mice under normal conditions and after exposure to ototoxic drugs.

    abstract::The expression of the intermediate filament subclasses cytokeratins (CKs), vimentin, and neurofilament triplet proteins (NFs) was analysed in the embryonic inner ear of the CBA/CBA mouse, in vivo and in vitro, using well-defined monoclonal antibodies. Some of the cultured inner ears were exposed to 1-10 micrograms/ml ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489009107415

    authors: Berggren D,Anniko M,Thornell LE,Ramaekers FC,Virtanen I

    更新日期:1990-01-01 00:00:00

  • Tracheal transplantation: demonstration of HLA class II subregion gene products on human trachea.

    abstract::The present morphological study was designed to investigate the expression of HLA class II subregion gene products on human trachea. Frozen sections from the tracheas of 20 cadavers not suffering from ear, nose or throat diseases were studied immunohistochemically using monoclonal antibodies which recognize monomorphi...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489009122530

    authors: Bujia J,Wilmes E,Hammer C,Kastenbauer E

    更新日期:1990-07-01 00:00:00

  • Olfactory dysfunction in patients after recovering from COVID-19.

    abstract:BACKGROUND:Smell and taste disorders occur in COVID-19 with a high prevalence, but little is known about the duration of the symptoms. In particular, studies using validated olfactory tests are very rare to date. AIMS/OBJECTIVES:The aim of this study was to determine the olfactory function of COVID-19 recoveries by a ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016489.2020.1811999

    authors: Otte MS,Eckel HNC,Poluschkin L,Klussmann JP,Luers JC

    更新日期:2020-12-01 00:00:00

  • Localization of nontypeable Haemophilus influenzae endotoxin in the middle and inner ear during experimental otitis media.

    abstract::Nontypeable Haemophilus influenzae (NTHi) lipooligosaccharide, the major component of H. influenzae endotoxin, was localized in the middle and inner ear subsequent to the resolution of experimental otitis media induced by this pathogen. A monoclonal antibody specific for the lipooligosaccharide of this strain was used...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016489950180838

    authors: Demaria TF

    更新日期:1999-01-01 00:00:00

  • Familial early-onset progressive vestibulopathy without hearing impairment.

    abstract::A family with early-onset vestibulopathy is presented. The 34-year-old father had experienced brief attacks of vertigo and currently suffers from unsteadiness and oscillopsia during head movements. His two young sons also experience brief attacks of spontaneous vertigo. In vestibular testing all three subjects showed ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016480310002500

    authors: Brantberg K

    更新日期:2003-08-01 00:00:00

  • Prediction of upper respiratory tract bacteria in acute otitis media.

    abstract:CONCLUSIONS:Thorough otomicroscopical examination of the tympanic membrane in acute otitis media (AOM) might distinguish AOM episodes caused by different bacteria. It thus might be a way to select appropriate treatment for each patient without raising the number of dangerous complications. OBJECTIVES:The aim of this s...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016480601091719

    authors: Foglé-Hansson M,White P,Hermansson A

    更新日期:2007-09-01 00:00:00

  • Imaging of cochlear implant electrode array with flat-detector CT and conventional multislice CT: comparison of image quality and radiation dose.

    abstract:CONCLUSION:Cochlear implantation assessment is possible using commercially available standard flat-detector computed tomography (FD-CT) protocols. Image quality is superior to multislice CT (MSCT). The radiation dose of FD-CT is lower in comparison with MSCT standard protocols and may therefore overcome the limitations...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016480903292700

    authors: Struffert T,Hertel V,Kyriakou Y,Krause J,Engelhorn T,Schick B,Iro H,Hornung J,Doerfler A

    更新日期:2010-04-01 00:00:00

  • An external neck brace to support the peristomal fixation of an automatic stoma valve (ASV): 3D stereophotogrammetrical assessment.

    abstract:CONCLUSION:The external neck brace (ENB) is a new concept in the improvement of peristomal fixation of an automatic stoma valve (ASV), which was evaluated with a new imaging method. The addition of the ENB to the Flexiderm (FLD) and Xtrabase (XB) adhesives significantly reduced absolute mean differences between the spe...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016480903426600

    authors: Dirven R,Kooijman PG,Maal TJ,Hilgers FJ,Bergé SJ,Marres HA

    更新日期:2010-07-01 00:00:00

  • Extracellular ATP-induced Ca2+ mobilization of type I spiral ganglion cells from the guinea pig cochlea.

    abstract::Intracellular calcium concentrations ([Ca2+]i) in type I cochlear spiral ganglion cells (SGCs) of the guinea pig were measured by digital imaging microscopy and the Ca(2+)-sensitive dye Fura-2. Extracellular ATP induced elevation of [Ca2+]i in type I SGCs in a concentration-dependent manner. The ATP-induced elevation ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489709113435

    authors: Cho H,Harada N,Yamashita T

    更新日期:1997-07-01 00:00:00

  • Non-sentinel node tumor invasion in oropharyngeal and oral cancer: risk of misdiagnosis of metastasis.

    abstract:CONCLUSION:The existence of patients with positive non-sentinel node indicates a risk of misdiagnosis of metastasis in oropharyngeal and oral cancer. OBJECTIVES:We attempted to confirm the usefulness of sentinel lymph node biopsy (SLNB) in oropharyngeal and oral cavity cancer to detect clinically occult metastases. P...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016480801891710

    authors: Santaolalla F,Sanchez JM,Ereño C,Gonzalez A,Rodriguez ML,Sanchez A,Martinez A

    更新日期:2008-10-01 00:00:00

  • The Advanced Bionics High Resolution Mode: stimulation rates up to 5000 pps.

    abstract:CONCLUSION:The technology of stimulation rates between 1500 and 5000 pulses per second per channel (pps/ch) brings a benefit for all users. However, the fastest possible rate does not necessarily lead to best hearing performance in each individual subject. Individual optimization of stimulation rate is recommended. OB...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016480902971239

    authors: Buechner A,Frohne-Büchner C,Gaertner L,Stoever T,Battmer RD,Lenarz T

    更新日期:2010-01-01 00:00:00

  • Glial reaction after facial nerve compression in the facial canal of the albino rat.

    abstract::The facial nerve of the albino rat was compressed by inserting a thin nylon thread in the facial canal. After a survival period of 1-60 days, the animal was perfused with Ringer's solution followed by a 2% periodate-lysine-paraformaldehyde fixative. Frozen sections from the brain stem containing the facial motor nucle...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489409126055

    authors: Ruan RS,Leong SK,Yeoh KH

    更新日期:1994-05-01 00:00:00

  • Source and role of endolymph macromolecules.

    abstract::Evaluation of some 200 endolymph proteins indicates that they are predominantly derived from plasma. However, the profile of endolymph proteins is remarkably similar to that of perilymph and entirely different from that of plasma. This supports the current consensus that perilymph rather than plasma is the (direct) so...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章,评审

    doi:10.1080/00016489950181260

    authors: Thalmann R,Thalmann I

    更新日期:1999-01-01 00:00:00

  • A comparison between nitric oxide output in the nose and sinuses: A pilot study in one volunteer.

    abstract::From a study of nitric oxide (NO) output in the nose and sinuses it seems that: (i) the results obtained regarding the regulation of NO output in the nose do not necessarily apply to the sinuses; (ii) the results obtained for one group of sinuses may not apply to another; and (iii) NO output in the sinuses does not be...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016480510031524

    authors: Qian W,Mäkitie AA,Witterick I,Haight JS

    更新日期:2005-10-01 00:00:00

  • Development and validation of the Mandarin disyllable recognition test.

    abstract:CONCLUSION:Given the phonetic balancing across lists and the validation with spectrally degraded speech, the present Mandarin disyllable recognition test (DRT) materials may be useful for assessing speech performance of Mandarin-speaking cochlear implant (CI) users. If combined with the previously developed sentence ma...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489.2011.653668

    authors: Zhu M,Wang X,Fu QJ

    更新日期:2012-08-01 00:00:00

  • Posture and nasal geometry.

    abstract::The influence of posture on nasal cavity geometry was studied by acoustic rhinometry in 14 normal adult subjects. On adopting a supine position from sitting the volume of the initially more patulous nasal cavity decreased and the volume of the less patulous side increased. These changes in volume are statistically sig...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489309135858

    authors: O'Flynn P

    更新日期:1993-07-01 00:00:00

  • Progressive degeneration in the cochlear nucleus after chemical destruction of the cochlea.

    abstract::In the guinea pig, the organ of Corti and the spiral ganglion cells were destroyed by administering gentamicin into the inner ear. The antero-ventral cochlear nucleus (AVCN) was studied with electron microscopy 15 and 30 days after treatment. There does not seem to be a specific type of degeneration induced by the dru...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016488609108643

    authors: Koitchev K,Aran JM,Ivanov E,Cazals Y

    更新日期:1986-07-01 00:00:00

  • A simple objective evaluation and grading for facial paralysis outcomes.

    abstract:CONCLUSION:A simple measuring box to determine lip-shift angle and eye-width narrowing offered simultaneous and objective data on outcome dysfunction. The method is as easy as visual approaches. The method is objective and as easy as visual access. It is practical for routine clinical use in any clinic. OBJECTIVES:The...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489.2011.626793

    authors: Saito H

    更新日期:2012-01-01 00:00:00

  • Correlation between severity of sleep apnea and upper airway morphology: Cephalometry and MD-CT study during awake and sleep states.

    abstract:CONCLUSION:The data show that the evaluation of obstruction site in patients with obstructive sleep apnea (OSA) should be performed in the sleep state rather than in wakefulness. OBJECTIVE:The aim of this study was to identify correlation between severity of OSA as measured by the apnea-hypopnea index (AHI) and upper ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489.2010.514007

    authors: Heo JY,Kim JS

    更新日期:2011-01-01 00:00:00

  • Strial prostaglandins and leukotrienes. Biochemical characteristics and interrelationship with furosemide.

    abstract::Synthesis of prostaglandins (PGs) was characterized in the lateral wall (LW) of guinea-pig cochlea. Basal synthesis at 37 degrees C was about 480 pg/LW (12.8 ng X mg-1 protein) for PGI2 and 85 pg/LW (2.3 ng X mg-1 protein) for PGE2, levelling out after 10 min of incubation. Incubation with arachidonic acid (10(-5) M) ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:

    authors: Tran Ba Huy P,Ferrary E,Escoubet B,Sterkers O

    更新日期:1987-05-01 00:00:00

  • Effects of aging on normal hearing loss and noise-induced threshold shift in albino and pigmented guinea pigs.

    abstract::In a previous investigation into noise-induced hearing loss by comparing 2-month-old albino with pigmented guinea pigs, albinos displayed significantly greater shifts in cochlear microphonic (CM) threshold and less recovery than the pigmented animals 7 days after noise exposure. The present study compared the response...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016488809107372

    authors: Conlee JW,Abdul-Baqi KJ,McCandless GA,Creel DJ

    更新日期:1988-07-01 00:00:00

  • Outcome of surgical management of laryngeal carcinoma: a 5-year experience.

    abstract:OBJECTIVE:To investigate the outcome of surgically managed laryngeal carcinoma patients attending an outpatient clinic over a 5-year period. MATERIAL AND METHODS:This was an analytical, retrospective, cross-sectional study. A total of 36 laryngeal carcinoma patients who were managed surgically with or without radiothe...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016480410018070

    authors: Baskota DK,Prasad R,Sinha BK,Amatya RC,Bhattarai H,Guragain RP

    更新日期:2004-08-01 00:00:00

  • Nasal peak flow rate records in work related nasal blockage.

    abstract::Eleven patients, who had symptoms of blocking nose in relation to exposure to airway irritants in their work environment, and 11 control subjects recorded nasal and bronchial peak expiratory flow rates (PEFRN and PEFRB) during a working week. In contrast to the control subjects the patients demonstrated a pattern of g...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:

    authors: Ahman M

    更新日期:1992-09-01 00:00:00

  • Otolith function tests in patients with unilateral vestibular lesions.

    abstract::This study was designed to assess the utility of otolith-ocular reflex testing in patients with complete unilateral peripheral vestibular deficits. Ten subjects were given a clinical vestibular test battery (ENG & vertical rotation) and research oriented tests of otolith function including dynamic posturography with h...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489509139392

    authors: Payman RN,Wall C 3rd,Ash-Bernal R

    更新日期:1995-11-01 00:00:00

  • Induction of MUC5AC mucin expression by histamine through the activation of its core promoter region.

    abstract:CONCLUSION:This study provides evidence that histamine induced MUC5AC mRNA expression through the activation of the core region of its promoter. It may also help in approaching new therapeutic strategies in airway mucins hypersecretory diseases. OBJECTIVE:Mucin hypersecretion characterizes several respiratory diseases...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489.2015.1017890

    authors: Xu F,Zhang L,Gao J,Wang D

    更新日期:2015-07-01 00:00:00

  • The effect of external stimuli on rat middle ear epithelium in culture. Extracellular carbon dioxide concentration.

    abstract::Serially cultivated rat middle ear epithelium was studied under exposure to various concentrations of carbon dioxide. Three experiments were performed exposing the epithelium to a gaseous environment containing 5 or 10% CO2 or to an atmosphere almost devoid of CO2. Oxygen was maintained constant at 20% and nitrogen wa...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489009125165

    authors: Lutgert HW,van Blitterswijk CA,Grote JJ

    更新日期:1990-05-01 00:00:00

  • Human papillomavirus (HPV)-positive tonsillar carcinomas are frequent and have a favourable prognosis in males in Norway.

    abstract:CONCLUSIONS:This study confirms a high prevalence of human papillomavirus (HPV)-positive tonsillar tumours (52%). The survival of the HPV-positive group was significantly better in males. OBJECTIVES:We assessed the prevalence of HPV in 137 patients with tonsillar carcinomas, measured the p53- and Ki-67-positive tumour...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016480903071377

    authors: Hannisdal K,Schjølberg A,De Angelis PM,Boysen M,Clausen OP

    更新日期:2010-02-01 00:00:00

  • Vestibular and optokinetic responses of the white cat.

    abstract::The vestibular and optokinetic responses of a group of white cats were evaluated and compared with the responses from a control group of pigmented animals. The results indicate that in all cases the white cats exhibited varying degrees of vestibular and/or optokinetic dysfunction, which in some animals varied from tes...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016487709123944

    authors: Waldorf RA,Polsinelli DJ,Knister JR,Kohut RI

    更新日期:1977-07-01 00:00:00

  • Ultrastructural changes of the basement membrane zone in benign lesions of the vocal folds.

    abstract::The basement membrane zone (BMZ) of the epithelium of the vocal folds was investigated electron microscopically in 10 patients suffering from various benign lesions and in 3 controls. Various defects were observed: a thickening by deposition of electron dense material, a loss of normal architecture, and a near absence...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489309135774

    authors: Dikkers FG,Hulstaert CE,Oosterbaan JA,Cervera-Paz FJ

    更新日期:1993-01-01 00:00:00

  • Eye movements and motion perception induced by off-vertical axis rotation (OVAR) at small angles of tilt after spaceflight.

    abstract::The nystagmus and motion perception of two astronauts were recorded during Earth-vertical axis rotation and during off-vertical axis rotation (OVAR) before and after 7 days of spaceflight. Postflight, the peak velocity and duration of per- and postrotatory nystagmus during velocity steps about the Earth-vertical axis ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489509139374

    authors: Clement G,Darlot C,Petropoulos A,Berthoz A

    更新日期:1995-09-01 00:00:00