Abstract:
CONCLUSION:Videomicroscopy with subpixel analysis is an excellent system for quantification of outer hair cell (OHC) movements. The resolution of a few nanometers is accurate enough to show induced differences of electromotility. OBJECTIVE:Electromotility of OHCs is a voltage-dependent process resulting from a membrane protein named prestin. Voltage sensitivity is conferred to prestin by intracellular anions. Reduction of these anions reduces electromotility. Videomicroscopy and subpixel tracking combine video-based analysis with a resolution of few nanometers. The aim of this study was to show the feasibility of a system for quantification of OHC movements. MATERIALS AND METHODS:Electromotility was investigated under normal and reduced intracellular chloride conditions. Cells were stimulated by the patch-clamp technique. Voltage steps were 500 ms long, ranging from -170 to +30 mV in 10 mV steps. RESULTS:As in previous studies our results show the following. The direction of OHC movement depends on the polarity of voltage steps, length changes are not equal for symmetrical voltage steps of opposite polarity, average shortening for a depolarizing step (-70 mV to +30 mV) is about 13 nm/mV. Hyperpolarization (-70 mV to -170 mV) on average evokes elongations of about 3 nm/mV. Half maximal chloride concentration reduces motility by 14%; half maximal electromotility is reached by a 94% reduction of chloride.
journal_name
Acta Otolaryngoljournal_title
Acta oto-laryngologicaauthors
Canis M,Ortner M,Olzowy B,Jahn K,Strupp M,Hemmert W,Berghaus A,Suckfuell Mdoi
10.1080/00016480701411502subject
Has Abstractpub_date
2008-03-01 00:00:00pages
228-32issue
3eissn
0001-6489issn
1651-2251pii
788418452journal_volume
128pub_type
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journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489009107415
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doi:10.1080/00016489950180838
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