Extracellular ATP-induced Ca2+ mobilization of type I spiral ganglion cells from the guinea pig cochlea.

Abstract:

:Intracellular calcium concentrations ([Ca2+]i) in type I cochlear spiral ganglion cells (SGCs) of the guinea pig were measured by digital imaging microscopy and the Ca(2+)-sensitive dye Fura-2. Extracellular ATP induced elevation of [Ca2+]i in type I SGCs in a concentration-dependent manner. The ATP-induced elevation of [Ca2+]i in SGC was even evident in the Ca(2+)-free solution, thereby suggesting that ATP induces a Ca(2+)-release from intracellular stores in SGCs. Suramin and reactive blue 2, both antagonists for the P2-purinergic receptor, inhibited the [Ca2+]i increase in SGCs induced by extracellular ATP in a dose-dependent manner. Adenosine did not induce any changes of [Ca2+]i in SGC. These results suggest that type I SGCs may possess P2-purinergic receptor but not P1-purinergic receptor. Extracellular ATP induced a [Ca2+]i increase in type I SGCs, with and without neuritic processes, while L-glutamate increased [Ca2+]i in type I SGCs with neuritic processes, but not SGCs without neuritic processes. The ATP-induced [Ca2+]i increase was almost equal in both soma and processes. Therefore, the distribution of P2-purinergic receptor in type I SGCs may be homogeneous in soma and processes. Based on these observations, we suggest that extracellular ATP may act as a neurotransmitter or neuromodulator of the hair cell-afferent nerve synapse in the guinea pig cochlea.

journal_name

Acta Otolaryngol

journal_title

Acta oto-laryngologica

authors

Cho H,Harada N,Yamashita T

doi

10.3109/00016489709113435

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

545-52

issue

4

eissn

0001-6489

issn

1651-2251

journal_volume

117

pub_type

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