Prosaposin-derived peptide alleviates ischaemia-induced hearing loss.

Abstract:

CONCLUSION:An 18-mer peptide derived from the neurotrophic region of prosaposin (PS-pep) prevents hearing loss and cochlear damage due to transient cochlear ischaemia by activating an anti-apoptotic pathway. PS-pep is a potent candidate molecule for alleviating ischaemia-induced hearing loss. OBJECTIVE:PS-pep was investigated for its protective effects against ischaemia-induced hearing loss and cochlear damage. METHODS:Ischaemia was induced in both cochleae in Mongolian gerbils by pulling the ligatures around both vertebral arteries in an anterior direction using 5 g weights for 15 min. PS-pep was synthesized artificially and administered subcutaneously four times after the induction of transient cochlear ischaemia. RESULTS:An increase in the auditory brainstem response threshold was alleviated in animals treated with 2.0 mg/kg PS-pep. Histological examinations conducted on day 7 showed that the loss of inner hair cells (IHCs) was more prominent than that of outer hair cells. Higher doses of PS-pep significantly alleviated IHC loss. An increase in the anti-apoptotic factor bcl-2 was also noted in the IHCs treated with PS-pep.

journal_name

Acta Otolaryngol

journal_title

Acta oto-laryngologica

authors

Terashita T,Saito S,Nabeka H,Hato N,Wakisaka H,Shimokawa T,Kobayashi N,Gyo K,Matsuda S

doi

10.3109/00016489.2012.750430

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

462-8

issue

5

eissn

0001-6489

issn

1651-2251

journal_volume

133

pub_type

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