The effect of external stimuli on rat middle ear epithelium in culture. Extracellular carbon dioxide concentration.

Abstract:

:Serially cultivated rat middle ear epithelium was studied under exposure to various concentrations of carbon dioxide. Three experiments were performed exposing the epithelium to a gaseous environment containing 5 or 10% CO2 or to an atmosphere almost devoid of CO2. Oxygen was maintained constant at 20% and nitrogen was additionally supplied. Cells exposed at 0% CO2 showed a remarkably different morphology compared with the cultures at 5 and 10% CO2, cells could become extremely large at 0% CO2, also showing a great variation in shape and cell size within the culture. Proliferation was significantly impaired at 0% CO2 vis-à-vis the 5 and 10% experiments. Terminal differentiation, however, was not influenced by the different CO2 concentrations, indicating that in vitro, variation in CO2 concentration is of no importance regarding a possible differentiation of middle ear epithelium into a cornifying epithelium.

journal_name

Acta Otolaryngol

journal_title

Acta oto-laryngologica

authors

Lutgert HW,van Blitterswijk CA,Grote JJ

doi

10.3109/00016489009125165

subject

Has Abstract

pub_date

1990-05-01 00:00:00

pages

431-7

issue

5-6

eissn

0001-6489

issn

1651-2251

journal_volume

109

pub_type

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