Protein structure classification using geometric invariants and dynamic programming.

abstract：:Kallikrein-related peptidases (KLKs) are trypsin-like and chymotrypsin-like serine proteases which are expressed in several tissues. Their activity is tightly controlled by inhibitors including members of the serine protease Kazal-type (SPINK) family. These enzymes are promising targets for the treatment of skin desqu...

journal_title：Protein and peptide letters

authors： Assis DM,Zalazar L,Juliano MA,De Castro R,Cesari A

更新日期：2013-10-01 00:00:00

abstract：:Tumor vasculatures express high levels of α(V)β(3)/α(V)β(5) and α(5)β(1) integrins. Peptide containing the RGD (Arg-Gly-Asp) sequence, which is present in ligands of integrins, is effective in targeting therapeutic reagents to tumor vascular endothelium. In this study, we investigated whether the biological activity o...

journal_title：Protein and peptide letters

authors： Zhang HT,Li HC,Li ZW,Guo CH,Chen YJ

更新日期：2011-06-15 00:00:00

abstract：:Bacterial ADP-ribosyltransferases (BADPRTs) are extensively contributed to determine the strain-specific virulence state and pathogenesis in human hosts. Understanding molecular evolution and functional diversity of the BADPRTs is an important standpoint to describe the fundamental behind in the vaccine designing for ...

journal_title：Protein and peptide letters

authors： Chellapandi P,Sakthishree S,Bharathi M

更新日期：2013-09-01 00:00:00

abstract：:We report a new prohormone convertase gene of insect origin, Trichoplusia ni furin, which was cloned from BTI-Tn-5B-4 (Tn5) insect cells. We constructed a truncated mutant that lacked Cys-rich repeated segments. Using baculovirus expression system and standard enzymatic assay, we obtained recombinant Tn furin and eval...

journal_title：Protein and peptide letters

authors： Wang L,Xu D,Qian M,Yang G,Wu X

更新日期：2006-01-01 00:00:00

abstract：:In this article we review the current status of our understanding of membrane mediated interactions from theory and experiment. Phenomenological mean field and molecular models will be discussed and compared to recent experimental results from dynamical neutron scattering and atomic force microscopy. ...

journal_title：Protein and peptide letters

authors： Armstrong CL,Sandqvist E,Rheinstädter MC

更新日期：2011-04-01 00:00:00

abstract：:For biophysical studies using heteronuclear NMR analysis of amyloid beta peptide, construction of an efficient and high yield expression system of uniformly isotopic labeled amyloid beta peptide is desirable. Here we succeeded in obtaining (15)N-labeled amyloid beta 1-40 expressed by attachment to hen egg white lysozy...

journal_title：Protein and peptide letters

authors： Nagata-Uchiyama M,Yaguchi M,Hirano Y,Ueda T

更新日期：2007-01-01 00:00:00

abstract：:In general, halophilic proteins are stable only in the presence of salts at high concentrations. Not only is high salt concentration important for structural stability of halophilic proteins, but also refolding of a denatured halophilic protein requires high salt concentration. This review summarizes the importance of...

journal_title：Protein and peptide letters

authors： Arakawa T,Tokunaga M

更新日期：2004-04-01 00:00:00

abstract：:We present the results of a study on the influence of albumin and prolactin concentrations and intravascular fluid pH on vein permeability for albumin. Permeability conditions were simulated depending on albumin concentration, pH value and prolactin concentration. In research model an in vitro method was applied using...

journal_title：Protein and peptide letters

authors： Dolińska B,Caban A,Cierpka L,Ryszka F

更新日期：2014-01-01 00:00:00

abstract：:The 5S subunit of transcarboxylase was expressed and purified. Recent methods of NMR spectroscopy as transferred NOESY, INPHARMA and Saturation Transfer Difference (STD) NMR were used to investigate ligand binding of free biotin to the 5S protein. The binding epitope for biotin is very similar to that obtained at the ...

journal_title：Protein and peptide letters

authors： Bhat RK,Berger S

更新日期：2008-01-01 00:00:00

abstract：:After consideration of the tertiary structure of the pY+3 binding pocket in SH2 domains, isoleucine of Ac-pYEEIE was replaced with various unnatural aliphatic amino acids and the binding affinities for Lck, Src, and Fyn SH2 domains were measured. We determined the characteristics of the amino acid at the pY+3 position...

journal_title：Protein and peptide letters

authors： Lee KH,Park SH

更新日期：2004-12-01 00:00:00

abstract：:An alternative method for expressing the recombinant proteins in Escherichia coli is proposed. Unlike the ordinary induction protocol where the cells in the early- to mid-log phase are induced for the protein production, this alternative protocol utilizes the cells in the stationary phase. By using a glutathione S-tra...

journal_title：Protein and peptide letters

authors： Chae YK,Cho KS,Chun W,Lee K

更新日期：2003-08-01 00:00:00

abstract：:A novel cellulase was isolated from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius ATCC27009 grown in medium containing carboxymethylcellulose. The enzyme is a glycosylated monomer of 56.2 kDa, relatively thermostable, with optimal pH and temperature of 4.0 and 65 degrees C, respectively. Enzymatic as...

journal_title：Protein and peptide letters

authors： Morana A,Esposito A,Maurelli L,Ruggiero G,Ionata E,Rossi M,La Cara F

更新日期：2008-01-01 00:00:00

abstract：:The Saccaromices cerevisiae D-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. It catalyses the conversion of D-serine into pyruvate and ammonia with the K(m) and k(cat) values of 0.39 mM and 13.1 s(-1) respectively. In this work, a new methodology for monitoring D-s...

journal_title：Protein and peptide letters

authors： Staiano M,Strianese M,Varriale A,Di Giovanni S,di Mase DS,Dell'Angelo V,Ruggiero G,Labella T,Pellecchia C,D'Auria S

更新日期：2012-06-01 00:00:00

abstract：:Kappa-conotoxin RIIIJ is a conopeptide to inhibit voltage-gated potassium channels, however, its detailed folding structures have yet to be studied. With the advance in computing power, it is possible to use the HP model to analyze all its possible folding structures. In this study, the amino acid sequences of kappa-c...

journal_title：Protein and peptide letters

authors： Yan S,Wu G

更新日期：2012-05-01 00:00:00

abstract：:Ewing's Sarcoma transcript-2 (EAT2) also known as SH2D1B is involved in regulation of signalling lymphocytic activation molecule (SLAM) family receptor functions. Cytoplasmic tails of SLAM family receptors contain tyrosine residues which mediate the downstream signal transduction through their phosphorylation. EAT2, c...

journal_title：Protein and peptide letters

authors： Taha M,Nezerwa E,Nam HJ

更新日期：2016-01-01 00:00:00

abstract：:We previously described a potent anthrax toxin inhibitor, based on a phage-library-selected peptide sequence, synthesized as a tetra-branched molecule on a lysine core and further modified for improvement of activity [Pini et al., Biochem. J., 2006, 395, 157]. This branched peptide had very low solubility because of s...

journal_title：Protein and peptide letters

authors： Pini A,Brunetti J,Falciani C,Fabbrini M,Bracci L

更新日期：2008-01-01 00:00:00

abstract：:To investigate the processing of transforming growth factor beta1 (TGFbeta1) pro-protein by furin protease we expressed a GST-pro-TGFbeta1 fusion protein in bacteria. Analysis of the furin digestion pattern revealed the liberation of 12.5 kDa TGFbeta1 monomers. There was no evidence for cleavage of an alternative furi...

journal_title：Protein and peptide letters

authors： Kahle NA,Joffroy C,Popp SL,Knabbe C,Stope MB

更新日期：2010-04-01 00:00:00

abstract：:A recombinant human parathyroid hormone (rhPTH) fragment (Gly1-Gln26-rhPTH(1-34)) which contains two amino acids substitutions (Gly1 and Gln26) was acquired through Escherichia coli expression system using a soluble fusion protein strategy. The soluble fusion protein MBP-Gly1-Gln26-rhPTH(1-34) was harvested after puri...

journal_title：Protein and peptide letters

authors： Xu XC,Zhong SD,Kai F,Li LR,Liu C,Liu B,Bao JK

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:NMAAP1 plays a role in regulating macrophage differentiation to the M1 type and exerting antitumoral functions. It is not clear what role and mechanism NMAAP1 does play in the reversal of macrophages from M1 to M2. METHODS:We detected the typing of macrophages with high or low expression of NMAAP1 by QPCR a...

journal_title：Protein and peptide letters

authors： Liu Q,Zhu P,Liu S,Tang M,Wang Y,Tian Y,Jin Z,Li D,Yan D

更新日期：2019-01-01 00:00:00

abstract：UNLABELLED:In this investigation a novel approach based on mucoadhesive superporous hydrogel hybrids (SPHHs) for topi- cal vaginal drug delivery for Metronidazole was tried. METHOD:SPHHs were synthesized by solution polymerization tech- nique. The synthesized SPHHs were characterized for tensile strength, swelling beh...

journal_title：Protein and peptide letters

authors： Chavda H,Chavada G,Patel J,Rangpadiya K,Patel C

更新日期：2014-01-01 00:00:00

abstract：:Michael Laskowski Jr. (1930-2004) was a pioneer in the field of Standard mechanism serine proteinase inhibitors. He made numerous important contributions in the field. This article highlights some of his most important contributions such as the discovery of the reactive site in serine proteinase inhibitors, the propos...

journal_title：Protein and peptide letters

authors： Qasim MA

更新日期：2005-07-01 00:00:00

abstract：:Adenoregulin is a 33 amino acid antibiotic peptide who belongs to dermaseptin family which is the first vertebrate family to show lethal effects against filamentous fungi, as well as a broad spectrum of pathogenic microorganisms. Synthetic adenoregulin gene was cloned in 2, 4 and 6 tandem repeats and subcloned in pET3...

journal_title：Protein and peptide letters

authors： Zhou Y,Cao W,Wang J,Ma Y,Wei D

更新日期：2005-05-01 00:00:00

abstract：:Class I fusion glycoproteins of viruses are involved in the fusion between viral envelope and cell membrane. A region located in the N-terminal domain of these glycoproteins, called the fusion peptide, is essential for fusion. Fusion peptides are able to induce by themselves in vitro membrane fusion. In this paper, we...

journal_title：Protein and peptide letters

authors： Charloteaux B,Lorin A,Brasseur R,Lins L

更新日期：2009-01-01 00:00:00

abstract：:Prediction of protein structure from its amino acid sequence is still a challenging problem. The complete physicochemical understanding of protein folding is essential for the accurate structure prediction. Knowledge of residue solvent accessibility gives useful insights into protein structure prediction and function ...

journal_title：Protein and peptide letters

authors： Pugalenthi G,Kandaswamy KK,Chou KC,Vivekanandan S,Kolatkar P

更新日期：2012-01-01 00:00:00

abstract：:Human butyrylcholinesterase (BuChE) is a serine enzyme present in most organs and plasma. No clear physiological function has yet been assigned to BuChE, but it is a pharmacologically and toxicologically important enzyme that plays a role in degradation of numerous ester-containing drugs and poisonous esters. Thus, Bu...

journal_title：Protein and peptide letters

authors： Masson P,Carletti E,Nachon F

更新日期：2009-01-01 00:00:00

abstract：:Hepatitis C virus and related viruses in the Flaviviridae family (such as dengue virus, yellow fever virus or West Nile virus) are amongst the most important human pathogens, causing substantial morbidity and mortality world-wide. The production of viral progeny in Flaviviridae is orchestrated by the small, multifunct...

journal_title：Protein and peptide letters

authors： Ivanyi-Nagy R,Darlix JL

更新日期：2010-08-01 00:00:00

abstract：:We used a de novo designed, beta-hairpin forming T1 peptide as a model to investigate the kinetics of peptide fibrogenesis by a combination of light scattering (LS), circular dichroism (CD), fluorescence, and atomic force microscopy (AFM). The results demonstrate that the T1 fibrogenesis undergoes a consecutive stepwi...

journal_title：Protein and peptide letters

authors： Wang C,Sha Y

更新日期：2010-04-01 00:00:00

abstract：:Like those in mammals, heterotrimeric G protein complexes have been implicated in signal transduction pathways in plants; however, the subunits themselves have not been isolated. In this study, the rice heterotrimeric G protein subunits α (Gα) and β (Gβ) were purified by affinity chromatography using anti-Gα and -Gβ a...

journal_title：Protein and peptide letters

authors： Abe Y,Matsusita K,Komatsu S,Iwasaki Y

更新日期：2012-03-01 00:00:00

abstract：:There is a growing body of evidence that the formation and accumulation of advanced glycation end products (AGE) have been known to progress under diabetic conditions, thereby being involved in diabetic vascular complications. Further, we, along with others, have recently found AGE could disturb insulin actions in cul...

journal_title：Protein and peptide letters

authors： Unoki-Kubota H,Yamagishi S,Takeuchi M,Bujo H,Saito Y

更新日期：2010-09-01 00:00:00

abstract：:Many polyketides show biological activities and have thus been applied in clinics, as food additives, and in agriculture. Type II thioesterases (TEIIs) play an important role in polyketide biosynthesis. Most TEIIs belong to α/β-hydrolase family and usually contain a catalytic triad Ser-His-Asp. In polyketide biosynthe...

journal_title：Protein and peptide letters

authors： Luo HD,Jin MY,Wu H,Jiang H

更新日期：2016-01-01 00:00:00