The 5S subunit of transcarboxylase interacts with free biotin as studied by transferred-NOESY and Saturation Transfer Difference NMR.

abstract：:Tumor vasculatures express high levels of α(V)β(3)/α(V)β(5) and α(5)β(1) integrins. Peptide containing the RGD (Arg-Gly-Asp) sequence, which is present in ligands of integrins, is effective in targeting therapeutic reagents to tumor vascular endothelium. In this study, we investigated whether the biological activity o...

journal_title：Protein and peptide letters

authors： Zhang HT,Li HC,Li ZW,Guo CH,Chen YJ

更新日期：2011-06-15 00:00:00

abstract：:Prediction of protein structure from its amino acid sequence is still a challenging problem. The complete physicochemical understanding of protein folding is essential for the accurate structure prediction. Knowledge of residue solvent accessibility gives useful insights into protein structure prediction and function ...

journal_title：Protein and peptide letters

authors： Pugalenthi G,Kandaswamy KK,Chou KC,Vivekanandan S,Kolatkar P

更新日期：2012-01-01 00:00:00

abstract：:The cyclotides are a large family of plant proteins that have a cyclic backbone and a knotted arrangement of three conserved disulfide bonds. Despite the apparent complexity of their cystine knot motif it is possible to efficiently fold these proteins, as exemplified by oxidative folding studies on the prototypic cycl...

journal_title：Protein and peptide letters

authors： Craik DJ,Daly NL

更新日期：2005-02-01 00:00:00

abstract：INTRODUCTION:The Renin-Angiotensin System (RAS) has emerged as being related to vascular disease. Recently the RAS has been associated with obesity, diabetes, and even cancer. OBJECTIVE:This review and Bioinformatics analyses focuses on the investigation of Angiotensinconverting enzymes (ACE and ACE2) as therapeutical...

journal_title：Protein and peptide letters

authors： de Carvalho Fraga CA,Farias LC,Jones KM,Batista de Paula AM,Guimaraes ALS

更新日期：2017-11-17 00:00:00

abstract：:A thermostable uricase identified in Bacillus firmus DWD-33, which was isolated for the first time from soil, with an apparent molecular weight of 33.5 kDa was stable against oxidants and SDS. The highest expression yields were obtained in medium containing 0.8% maltose and 1.2% soybean powder as carbon and nitrogen s...

journal_title：Protein and peptide letters

authors： Kotb E

更新日期：2015-01-01 00:00:00

abstract：:DNA fragmentation factor 45 (DFF45) regulates DNase DFF40 as its inhibitor and chaperone. It was reported that the N-terminal domain (NTD) of DFF45 alone is disordered and DFF40 is necessary as a mutual chaperone for the folding of NTD. However, here we reported the crystallization of DFF45 NTD. These crystals diffrac...

journal_title：Protein and peptide letters

authors： Li T,Li X,Yang W,Rao Z,Zhai Zh

更新日期：2003-04-01 00:00:00

abstract：:The studies presented here explore antimicrobial peptide preferential binding behavior for a target pathogen, Escherichia coli O157:H7. A modified immunoassay and surface plasmon resonance were employed to evaluate immobilized peptide binding of whole bacterial cells. The knowledge gained may guide the rational design...

journal_title：Protein and peptide letters

authors： Soares JW,Kirby R,Morin KM,Mello CM

更新日期：2008-01-01 00:00:00

abstract：:E. coli small heat shock proteins IbpA and IbpB (inclusion body binding proteins A and B) are known to act as holding chaperones on denaturing, aggregate-prone proteins. But, there is no clear understanding about which of the IbpA and IbpB has more holdase activity and how the holdase activity of one was influenced by...

journal_title：Protein and peptide letters

authors： Roy SS,Patra M,Nandy SK,Banik M,Dasgupta R,Basu T

更新日期：2014-06-01 00:00:00

abstract：:Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-e...

journal_title：Protein and peptide letters

authors： Liang J,Yang W,Meng X,Chen P,Du H

更新日期：2015-01-01 00:00:00

abstract：:Current advancements in biological protein discovery utilize bi-partite methods of fluorescence detection where chromophore and scaffold are uncoupled. One such technology, called fluorogen-activating proteins (FAPs), consists of single-chain-variable-fragments (scFvs) selected against small organic molecules (fluorog...

journal_title：Protein and peptide letters

authors： Gallo E,Wienbar S,Snyder AC,Vasilev KV,Armitage BA,Jarvik JW

更新日期：2014-01-01 00:00:00

abstract：:BmK ITa1 cDNA was cloned and highly expressed in E. coli and insect cell. SDS-PAGE and western blot analysis revealed that subunit molecular weight of expression products is about 40 kDa and 10 kDa respectively. The expression product purified by a Ni(2+)-IDA-sepharose 6B column was toxic for insect, which indicated t...

journal_title：Protein and peptide letters

authors： Liu Z,Yang GZ,Chi CW,Wu XF

更新日期：2002-10-01 00:00:00

abstract：:The outcome of protein crystallization attempts is often uncertain due to inherent features of the protein or to the crystallization process that are not fully under control of the experimentalist. The aim of this contribution is to propose user-friendly tools that can increase the success rate of a protein crytalliza...

journal_title：Protein and peptide letters

authors： Sánchez-Puig N,Sauter C,Lorber B,Giegé R,Moreno A

更新日期：2012-07-01 00:00:00

abstract：:Since their recent identification, eukaryotic Hsp70 nucleotide exchange factors (NEFs) have gained increasing interest due to their engagement in vital cellular processes. Here, I summarize our current knowledge of their mechanisms of action, regulations and cellular functions as well as their relevance for human dise...

journal_title：Protein and peptide letters

authors： Kabani M

更新日期：2009-01-01 00:00:00

abstract：:Increasing antibiotic resistance has led to an urgent need for new therapeutic approaches. Host defense peptides are known to be antimicrobial and have revealed broad immunomodulatory functions for both innate and adaptive immunity. This review will focus on the role of host defense peptides in infection and immune re...

journal_title：Protein and peptide letters

authors： Hirsch T,Jacobsen F,Steinau HU,Steinstraesser L

更新日期：2008-01-01 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is known to interact with different biomolecules and was implicated in many novel cellular activities including programmed cell death, nuclear RNA transport unrelated to the commonly known carbohydrate metabolism. We reported here the purification of GAPDH from Chironom...

journal_title：Protein and peptide letters

authors： Chong IK,Ho WS

更新日期：2013-09-01 00:00:00

abstract：:Application of complementary B and T cell epitopes in inducing anti-idiotypic and anti-clonotypic antibodies capable of regulating or suppressing the autoimmune responses in experimental autoimmune myasthenia gravis (EAMG), allergic neuritis (EAN) and allergic encephalomyelitis (EAE) has been the stimulus of many rese...

journal_title：Protein and peptide letters

authors： Sakarellos-Daitsiotis M,Cung MT,Sakarellos C,El Hilali Z,Kosmopoulou A,Voitharou C

更新日期：2004-08-01 00:00:00

abstract：:The stability of Merrifield linker in Fmoc deprotection process was quantitatively investigated by establishing working curve of two major decomposition components from two resin bound dipeptide models. By sampling reaction solution and analyzing with RP-HPLC, decomposition rate was determined. The results indicated t...

journal_title：Protein and peptide letters

authors： Yang X,Lin H,Lu W,Wang D

更新日期：2013-02-01 00:00:00

abstract：:Identification on protein folding types is always based on the 27-class folds dataset, which was provided by Ding & Dubchak in 2001. But with the avalanche of protein sequences, fold data is also expanding, so it will be the inevitable trend to improve the existing dataset and expand more folding types. In this paper,...

journal_title：Protein and peptide letters

authors： Liu L,Hu XZ,Liu XX,Wang Y,Li SB

更新日期：2012-04-01 00:00:00

abstract：BACKGROUND:Wheat germ, one of the byproducts of flour milling, contains abundant physiologically active components. Globulins in wheat germ are a class of high-quality functional proteins and have received widespread attention. However, the composition of wheat germ globulin（ WGG）and the structure of the typical protei...

journal_title：Protein and peptide letters

authors： Ji XG,Huang JH,Hui M,Zhang YQ,Zhao Y

更新日期：2018-02-08 00:00:00

abstract：BACKGROUND:Protealysin, a zinc metalloprotease of Serratia proteamaculans, is the prototype of a new group within the peptidase family M4. Protealysin-like proteases (PLPs) are widely spread in bacteria but are also found in fungi and archaea. The biological functions of PLPs have not been well studied, but published d...

journal_title：Protein and peptide letters

authors： Chukhontseva KN,Salnikov VV,Morenkov OS,Kostrov SV,Demidyuk IV

更新日期：2019-01-01 00:00:00

abstract：:There is accumulating evidence that proteinuria is not merely a biomarker for the progression of chronic kidney disease (CKD), but also a mediator of this devastating disorder. Indeed, albumin, one of the major components found in proteinuria, causes proinflammatory and profibrotic changes in cultured proximal tubular...

journal_title：Protein and peptide letters

authors： Matsui T,Yamagishi S,Ueda S,Fukami K,Okuda S

更新日期：2010-01-01 00:00:00

abstract：:In the development of cell-hybrid biomaterials, the functional activity of cells depends on the selective binding of cells to artificial ligands on the biomaterials. The extracellular matrix (ECM) is the most important ligand for cell activity. ECM is known to contain collagen, one of whose constituents is gelatin. Al...

journal_title：Protein and peptide letters

authors： Miyamoto K,Chinzei H,Komai T

更新日期：2002-12-01 00:00:00

abstract：:Currently, prion phenomena have been detected in various organisms, in addition to mammals affected by transmissible spongiform encephalopathies. In the budding yeast Saccharomyces cerevisiae, various proteins have prion properties and adopt atypical phenotypes as genetic elements, such as the Sup35 and Ure2 proteins,...

journal_title：Protein and peptide letters

authors： Inoue Y

更新日期：2009-01-01 00:00:00

abstract：:AB204 is an Activin/BMP2 chimera, which has been found to exhibit a higher activity than Bone Morphogenetic Protein 2 (BMP2) in osteogenic activity. To prepare AB204 for its preclinical studies, AB204 has been characterized in various formulation buffers. We observed that AB204 purified by ion-exchange chromatography ...

journal_title：Protein and peptide letters

authors： Ahn C,Maslennikov I,Choi JY,Oh H,Cheong C,Choe S

更新日期：2014-05-01 00:00:00

abstract：:Aspergillus sp. phytase contains five disulfide bonds. In order to elucidate their role, the reactivation and refolding of phytase in the absence and presence of dithiothreitol (DTT) was investigated. The results indicated that the disulfide bonds play an important role in the catalytic activity and conformational sta...

journal_title：Protein and peptide letters

authors： Song GY,Wang XY,Wang M

更新日期：2005-08-01 00:00:00

abstract：:The aerotolerance of the lactic - fermentative bacterium Streptococcus thermophilus is mainly based on the key antioxidant function of superoxide dismutase (StSOD). In this work, the comparison of recombinant StSOD (rStSOD) forms obtained from two different initiation triplets indicated that the enzyme from S. thermop...

journal_title：Protein and peptide letters

authors： De Vendittis A,Marco S,Di Maro A,Chambery A,Albino A,Masullo M,Michniewicz A,Parlato G,De Angelis A,De Vendittis E,Rullo R

更新日期：2012-03-01 00:00:00

abstract：:Kappa-conotoxin RIIIJ is a conopeptide to inhibit voltage-gated potassium channels, however, its detailed folding structures have yet to be studied. With the advance in computing power, it is possible to use the HP model to analyze all its possible folding structures. In this study, the amino acid sequences of kappa-c...

journal_title：Protein and peptide letters

authors： Yan S,Wu G

更新日期：2012-05-01 00:00:00

abstract：:Apomyoglobin (apoMb) folds through at least two partially folded forms that are detected both as transient intermediates during folding/unfolding kinetics or as stable intermediates at equilibrium. Here, I summarize the results of recent kinetic studies, which combined with detailed characterizations of equilibrium fo...

journal_title：Protein and peptide letters

authors： Jamin M

更新日期：2005-04-01 00:00:00

abstract：:Human butyrylcholinesterase (BuChE) is a serine enzyme present in most organs and plasma. No clear physiological function has yet been assigned to BuChE, but it is a pharmacologically and toxicologically important enzyme that plays a role in degradation of numerous ester-containing drugs and poisonous esters. Thus, Bu...

journal_title：Protein and peptide letters

authors： Masson P,Carletti E,Nachon F

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Cysteine S-sulfenylation is a major type of dynamic post-translational modification of the protein that plays an important role in regulating many biological processes in both of prokaryotic and eukaryotic species. To understand the function of S-sulfenylated proteins, identification of S-sulfenylation sites...

journal_title：Protein and peptide letters

authors： Hasan MM,Khatun MS,Kurata H

更新日期：2018-01-01 00:00:00