Study of colonization resistance for Enterobacteriaceae in man by experimental contamination and biotyping as well as the possible role of antibodies in the clearance of these bacteria from the intestines.

Abstract:

:The colonization resistance (CR) of the digestive tract was determined in 10 healthy volunteers by oral contamination with a neomycin resistant Escherichia coli (NR-E. coli) strain and measurement of the faecal concentration of this strain during 14 days after the contamination. This 'gold standard' was compared with another parameter of CR; the determination of the mean number of different biotypes of Enterobacteriaceae isolated from four faecal samples per volunteer. Both measures are significantly correlated (P less than 0.01). The NR-E. coli strain could be cultured from faecal samples of 4/10 volunteers as long as 300 days after contamination. Serum antibody titres against endogenous E. coli strains and the NR-E. coli strain used for experimental oral contamination were measured by an indirect immunofluorescence (IIF) assay. The assay was read by a video camera connected to an image processing system. The 95% confidence limits of antibody titres (log2) against endogenous E. coli strains ranged between less than 3 and 7.1 for IgA, between less than 3 and 8.7 for IgG and between less than 3 and 7.4 for IgM. Antibody titres against the NR-E. coli4 strain were within this (normal) range. The serum antibody titres against the NR-E. coli strain increased slowly after oral contamination, especially IgG and IgM. Little increase in IgA titres could be observed. An increase of serum antibody titres did not correlate with the elimination of the oral contaminant from the intestines. Therefore, we conclude that the CR is not IgG nor IgM antibody mediated.

journal_name

Epidemiol Infect

authors

Apperloo-Renkema HZ,van der Waaij D

doi

10.1017/s0950268800049311

subject

Has Abstract

pub_date

1991-12-01 00:00:00

pages

619-26

issue

3

eissn

0950-2688

issn

1469-4409

journal_volume

107

pub_type

杂志文章
  • Design and analysis of small-scale transmission experiments with animals.

    abstract::Interactions between pathogens and hosts at the population level should be considered when studying the effectiveness of control measures for infectious diseases. The advantage of doing transmission experiments compared to field studies is that they offer a controlled environment in which the effect of a single factor...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S095026880600673X

    authors: Velthuis AG,Bouma A,Katsma WE,Nodelijk G,De Jong MC

    更新日期:2007-02-01 00:00:00

  • Psittacosis outbreak after participation in a bird fair, Western France, December 2008.

    abstract::In December 2008, three hospitalized cases of suspected psittacosis infection were notified by respiratory disease clinicians from a local hospital to the Regional Epidemiology Unit of Pays de la Loire, France. They all had attended a bird fair. A retrospective cohort study was conducted among exhibitors and organizer...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268811000409

    authors: Belchior E,Barataud D,Ollivier R,Capek I,Laroucau K,de Barbeyrac B,Hubert B

    更新日期:2011-10-01 00:00:00

  • First detection and molecular characterization of Nebovirus in Brazil.

    abstract::Nebovirus is a new genus of viruses belonging to the Caliciviridae family recently characterized in cattle, and is associated with gastrointestinal disorders, such as diarrhoea, anorexia and intestinal lesions particularly in calves. The aim of this study was to investigate the prevalence of neboviruses in Brazilian c...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268816000029

    authors: Candido M,Alencar AL,Almeida-Queiroz SR,Buzinaro MG,Munin FS,Godoy SH,Livonesi MC,Fernandes AM,Sousa RL

    更新日期:2016-07-01 00:00:00

  • Characterization of a new genotype of measles virus detected in China and England.

    abstract::We report the co-circulation of a new lineage of measles virus (MV) and an Edmonston-like (Ed-like) genotype of MV in China during 1995-7. Sequence analysis of 25 strains was performed on a 282 nucleotides (nt) region of the nucleoprotein (N) gene, a 450-nt region of the haemagglutinin (H) gene and a 152-nt region of ...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s0950268898001666

    authors: Jin L,Sun YJ,Ge L,Brown DW

    更新日期:1998-12-01 00:00:00

  • Molecular epidemiology of endemic Clostridium difficile infection.

    abstract::This is the first study to provide a comprehensive insight into the molecular epidemiology of endemic Clostridium difficile and particularly that associated with a recently recognized epidemic strain. We DNA fingerprinted all C. difficile isolates from the stools of patients with symptomatic antibiotic-associated diar...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s095026880100557x

    authors: Fawley WN,Wilcox MH

    更新日期:2001-06-01 00:00:00

  • Quantifying homologous and heterologous antibody titre rises after influenza virus infection.

    abstract::Most influenza virus infections are associated with mild disease. One approach to estimate the occurrence of influenza virus infections in individuals is via repeated measurement of humoral antibody titres. We used baseline and convalescent antibody titres measured by haemagglutination inhibition (HI) and viral neutra...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268816000583

    authors: Freeman G,Perera RA,Ngan E,Fang VJ,Cauchemez S,Ip DK,Peiris JS,Cowling BJ

    更新日期:2016-08-01 00:00:00

  • Seroprevalence of SARS coronavirus antibody in household contacts.

    abstract::Between March and July 2003, 671 cases of severe acute respiratory syndrome (SARS) were diagnosed in Taiwan with a total of 84 fatalities. After the epidemic, a serological survey was conducted involving the asymptomatic household contacts. Household contacts of 13 index patients were enrolled in the study. Contact hi...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268805004541

    authors: Lee CC,Chen SY,Chang IJ,Tsai PC,Lu TC,Wu PL,Chen WJ,Huang LM,Chang SC

    更新日期:2005-12-01 00:00:00

  • Multistate foodborne disease outbreaks associated with raw tomatoes, United States, 1990-2010: a recurring public health problem.

    abstract::We examined multistate outbreaks attributed to raw tomatoes in the United States from 1990 to 2010. We summarized the demographic and epidemiological characteristics of 15 outbreaks resulting in 1959 illnesses, 384 hospitalizations, and three deaths. Most (80%) outbreaks were reported during 2000-2010; 73% occurred Ma...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268814002167

    authors: Bennett SD,Littrell KW,Hill TA,Mahovic M,Behravesh CB

    更新日期:2015-05-01 00:00:00

  • Antibodies against mumps in The Netherlands as assessed by indirect ELISA and virus neutralization assay.

    abstract::To obtain insight into mumps immunity 9 years after introduction of vaccination in The Netherlands, antibodies were measured in a national sample (n = 8298) and in clustered religious groups with low vaccine acceptance (n = 254). All sera were tested by indirect ELISA, and agreement with neutralization assay was asses...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s0950268803008641

    authors: van den Hof S,Beaumont MT,Berbers GA,de Melker HE

    更新日期:2003-08-01 00:00:00

  • The incidence of pneumonia using data from a computerized general practice database.

    abstract::Despite being widely recognized as a significant public health problem there are surprisingly few contemporary data available on the incidence of pneumonia in the UK. We conducted a general population-based cohort study to determine the incidence of pneumonia in general practice in the United Kingdom. Data were obtain...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268808001428

    authors: Myles PR,McKeever TM,Pogson Z,Smith CJ,Hubbard RB

    更新日期:2009-05-01 00:00:00

  • Numerical index of the discriminatory ability of biotyping and resistotyping for strains of Escherichia coli.

    abstract::A discrimination index was applied to assess the value of biotyping and resistotyping, used alone or together, for the subspecific discrimination of Escherichia coli. The index was high when 599 strains from a wide variety of sources were examined by full biotyping with 10 tests. Although the discrimination achieved w...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s095026880004975x

    authors: Crichton PB,Old DC

    更新日期:1992-04-01 00:00:00

  • Environmental sampling coupled with real-time PCR and genotyping to investigate the source of a Q fever outbreak in a work setting.

    abstract::A Q fever outbreak was declared in February 2016 in a company that manufactures hoists and chains and therefore with no apparent occupational-associated risk. Coxiella burnetii infection was diagnosed by serology in eight of the 29 workers of the company; seven of them had fever or flu-like signs and five had pneumoni...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268817000796

    authors: Hurtado A,Alonso E,Aspiritxaga I,López Etxaniz I,Ocabo B,Barandika JF,Fernández-Ortiz DE Murúa JI,Urbaneja F,Álvarez-Alonso R,Jado I,García-Pérez AL

    更新日期:2017-07-01 00:00:00

  • Analysis of some virulence factors of Vibrio vulnificus isolated from Rio de Janeiro, Brazil.

    abstract::Twenty strains of V. vulnificus isolated from the environment were investigated for characteristics related to their infectivity such as colonial morphology, enzymatic activity and animal assays. The presence of DNase, chitinase, amylase, lecithinase and gelatinase was observed in 100% of the strains, haemolytic activ...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s0950268800049979

    authors: Rodrigues DP,Ribeiro RV,Hofer E

    更新日期:1992-06-01 00:00:00

  • Influenza hospitalizations in Australian children.

    abstract::Australia's National Immunisation Program (NIP) provides free influenza vaccination for children at high risk of severe influenza; a pilot-funded programme for vaccine in all children aged 6 months to <5 years in one of eight states, has seen poor vaccine impact, related to recent vaccine safety concerns. This retrosp...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268816003381

    authors: Li-Kim-Moy J,Yin JK,Blyth CC,Kesson A,Booy R,Cheng AC,Macartney K

    更新日期:2017-05-01 00:00:00

  • Mumps outbreak in Israel's highly vaccinated society: are two doses enough?

    abstract::Mumps outbreaks in recent years have given rise to questions about the effectiveness of the mumps vaccine. This study examined the epidemiological data from a recent mumps outbreak in Israel and from outbreaks in other countries with high vaccination coverage, and considered whether long-established vaccination polici...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S095026881100063X

    authors: Anis E,Grotto I,Moerman L,Warshavsky B,Slater PE,Lev B

    更新日期:2012-03-01 00:00:00

  • Legionnaires' disease in France: sensitivity of the mandatory notification has improved over the last decade.

    abstract::The notification rate of Legionnaires' disease (LD) in France was 2.4/100 000 population in 2010, varying across regions with an increasing rate from west to east. Two sources [mandatory notifications (MN) and a survey of hospital laboratories] were used in a capture-recapture study to estimate the number of LD cases ...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268813000502

    authors: Campese C,Jarraud S,Sommen C,Maine C,Che D

    更新日期:2013-12-01 00:00:00

  • Incidence of surgical-site infections in orthopaedic surgery: a northern Italian experience.

    abstract::SUMMARYA prospective cohort study with a 1-year telephone follow-up was performed to estimate the incidence of surgical-site infections (SSIs) in hip and knee prostheses. Twenty-five public hospitals equipped with orthopaedic units in two Italian regions were involved. An 8-month surveillance period was set to obtain ...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268810001627

    authors: Castella A,Argentero PA,Farina EC,Charrier L,Del Prever EM,Zotti CM,Piemonte Nosocomial Infection Study Group.

    更新日期:2011-05-01 00:00:00

  • The effectiveness of Haemophilus influenzae type b conjugate vaccines in a high risk population measured using immunization register data.

    abstract::The Northern Territory of Australia has had historically very high incidence rates of invasive Haemophilus influenzae type b disease in children less than 5 years of age, with the burden of disease greatest among Aboriginal infants less than 12 months. This study documents the impact of conjugate Hib vaccines introduc...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s0950268801005155

    authors: Markey P,Krause V,Boslego JW,Coplan PM,Dargan JM,Kaplan KM

    更新日期:2001-02-01 00:00:00

  • Associations between water-treatment methods and diarrhoea in HIV-positive individuals.

    abstract::This manuscript extends our previously published work (based on data from one clinic) on the association between three drinking water-treatment modalities (boiling, filtering, and bottling) and diarrhoeal disease in HIV-positive persons by incorporating data from two additional clinics collected in the following year....

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s0950268802007422

    authors: Eisenberg JN,Wade TJ,Hubbard A,Abrams DI,Leiser RJ,Charles S,Vu M,Saha S,Wright CC,Levy DA,Jensen P,Colford JM Jr

    更新日期:2002-10-01 00:00:00

  • Increase in bloodstream infections in Finland, 1995-2002.

    abstract::A national, population-based laboratory surveillance of bloodstream infections (BSI) in Finland was performed. Blood-culturing rates were determined from data from clinical microbiology laboratories and trends in rates were evaluated using Poisson regression. During 1995-2002, 51,510 cases of BSI were notified; the an...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268807008138

    authors: Skogberg K,Lyytikäinen O,Ruutu P,Ollgren J,Nuorti JP

    更新日期:2008-01-01 00:00:00

  • Differences in the susceptibility of dromedary and Bactrian camels to foot-and-mouth disease virus.

    abstract::In this study, two sheep, eight dromedary camels and two Bactrian camels were inoculated with foot-and-mouth disease virus (FMDV) type A SAU 22/92. Five naive dromedary camels and four sheep were kept in direct or indirect contact with the inoculated camels. The inoculated sheep, which served as positive controls, dis...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268808001088

    authors: Larska M,Wernery U,Kinne J,Schuster R,Alexandersen G,Alexandersen S

    更新日期:2009-04-01 00:00:00

  • Priorities in global measles control: report of an outbreak in N'Djamena, Chad.

    abstract::In N'Djamena, capital of Chad, measles vaccination coverage of 12-23-month-old children fell from 61% in 1990 to 15% in 1993. A community survey of measles after an outbreak in 1993 showed that among children < 5 years of age, the mean monthly attack rate was 37 per 1,000 (95% CI, 32-43) and the mean case fatality rat...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s095026880005843x

    authors: Ndikuyeze A,Cook A,Cutts FT,Bennett S

    更新日期:1995-10-01 00:00:00

  • The prevalence of antimicrobial resistance in human faecal flora in South Africa.

    abstract::Between January and March 1992, 361 faecal specimens were collected from the healthy black population in the Transvaal Province of South Africa. Each specimen was examined for the prevalence of antimicrobial resistance in commensal bacteria. Volunteers, from both rural and urban dwellings, were divided into four age g...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s0950268800056922

    authors: Shanahan PM,Wylie BA,Adrian PV,Koornhof HJ,Thomson CJ,Amyes SG

    更新日期:1993-10-01 00:00:00

  • The human pathogenic vibrios--a public health update with environmental perspectives.

    abstract::Pathogenic Vibrio species are naturally-occurring bacteria in freshwater and saline aquatic environments. Counts of free-living bacteria in water are generally less than required to induce disease. Increases in number of organisms towards an infective dose can occur as water temperatures rise seasonally followed by gr...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章,评审

    doi:10.1017/s0950268800030326

    authors: West PA

    更新日期:1989-08-01 00:00:00

  • Methicillin-resistant Staphylococcus aureus (MRSA) in food production animals.

    abstract::Until recently, reports on methicillin-resistant Staphylococcus aureus (MRSA) in food production animals were mainly limited to occasional detections in dairy cattle mastitis. However, since 2005 a MRSA clone, CC398, has been reported colonizing pigs, veal calves and broiler chickens and infecting dairy cows. Many asp...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章,评审

    doi:10.1017/S0950268809991567

    authors: Vanderhaeghen W,Hermans K,Haesebrouck F,Butaye P

    更新日期:2010-05-01 00:00:00

  • Study of the effectiveness of influenza vaccination in the elderly in the epidemic of 1989-90 using a general practice database.

    abstract::The effectiveness of influenza vaccination in preventing serious illness and death was determined in an elderly population during the influenza epidemic of was determined in an elderly population during the influenza epidemic of was determined in an elderly population during the influenza epidemic of 1989-90. A retros...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s095026880005874x

    authors: Fleming DM,Watson JM,Nicholas S,Smith GE,Swan AV

    更新日期:1995-12-01 00:00:00

  • Experimental studies on the infectivity of non-culturable forms of Campylobacter spp. in chicks and mice.

    abstract::The significance of non-culturable forms of Campylobacter spp., especially with regard to the epidemiology of this organism in poultry flocks, was explored. Two different experiments were conducted to produce non-culturable Campylobacter spp. and test their ability to colonize the animal gut. In the first experiment a...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s0950268800059124

    authors: van de Giessen AW,Heuvelman CJ,Abee T,Hazeleger WC

    更新日期:1996-12-01 00:00:00

  • The seasonality of canine births and human campylobacteriosis: a hypothesis.

    abstract::The seasonality of canine births was investigated using records from the Kennel Club and the breeding centre for the Guide Dogs for the Blind Association. In these populations a distinct seasonal pattern was found with a greater number of puppies being born in the summer months than the winter. The hypothesis that the...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/s0950268800068199

    authors: Evans SJ

    更新日期:1993-04-01 00:00:00

  • A randomized controlled trial of vitamin D3 supplementation for the prevention of symptomatic upper respiratory tract infections.

    abstract::Vitamin D has been shown to be an important immune system regulator. Vitamin D insufficiency during winter may cause increased susceptibility to upper respiratory tract infections (URIs). To determine whether vitamin D supplementation during the winter season prevents or decreases URI symptoms, 162 adults were randomi...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章,随机对照试验

    doi:10.1017/S0950268809002404

    authors: Li-Ng M,Aloia JF,Pollack S,Cunha BA,Mikhail M,Yeh J,Berbari N

    更新日期:2009-10-01 00:00:00

  • The incidence of varicella and herpes zoster in Taiwan during a period of increasing varicella vaccine coverage, 2000-2008.

    abstract::The introduction and the widespread use of the varicella vaccine in Taiwan has led to a 75-80% decrease in the incidence of varicella in children. However the vaccine's long-term impact on the incidence of herpes zoster (HZ) has attracted attention. By controlling gender, underlying diseases, and age effects, a Poisso...

    journal_title:Epidemiology and infection

    pub_type: 杂志文章

    doi:10.1017/S0950268811001786

    authors: Chao DY,Chien YZ,Yeh YP,Hsu PS,Lian IB

    更新日期:2012-06-01 00:00:00