Abstract:
:The effect of oligosaccharides and C-terminal lysine residues on the thermal stability of a recombinant IgG(1) monoclonal antibody was investigated using differential scanning calorimetry (DSC). The C-terminal lysine did not appear to affect the thermal stability of this IgG(1) molecule. However, oligosaccharides, which are buried between the two CH(2) domains, provided significant stabilizing energy. Characterization of the Fab and Fc after papain digestion suggested that the stabilizing effect of oligosaccharides on this molecule was through stabilizing CH(2) domains. Oligosaccharides had little effect on the thermal stability of Fab region and CH(3) domains. It was also interesting to note that both intact IgG(1) antibody and its Fab, but not the Fc regions, appeared to form precipitate after thermal unfolding under the experimental conditions.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Liu H,Bulseco GG,Sun Jdoi
10.1016/j.imlet.2006.05.011subject
Has Abstractpub_date
2006-08-15 00:00:00pages
144-53issue
2eissn
0165-2478issn
1879-0542pii
S0165-2478(06)00151-9journal_volume
106pub_type
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journal_title:Immunology letters
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journal_title:Immunology letters
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pub_type: 杂志文章
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2003.05.001
更新日期:2003-10-31 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
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更新日期:2016-04-01 00:00:00
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2017.05.012
更新日期:2017-08-01 00:00:00
abstract::Multiple sclerosis (MS) is a debilitating neurological disorder of the central nervous system (CNS), characterized by activation and infiltration of leukocytes and dendritic cells into the CNS. In the initial phase of MS and its animal model, experimental autoimmune encephalomyelitis (EAE), peripheral macrophages infi...
journal_title:Immunology letters
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更新日期:2014-07-01 00:00:00
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journal_title:Immunology letters
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doi:10.1016/j.imlet.2016.04.011
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND:Omalizumab therapy was found to be safe and effective as an add-on therapy for patients with poorly controlled severe asthma. Although several studies over the last decade have demonstrated its efficacy in other Immunoglobulin E related diseases, its use in such conditions is off-label. OBJECTIVE:This study...
journal_title:Immunology letters
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更新日期:2020-11-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2013.05.002
更新日期:2013-05-01 00:00:00
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2011-01-30 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2015-07-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
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更新日期:1998-12-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00314-x
更新日期:2001-02-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2010.06.002
更新日期:2010-08-16 00:00:00