Abstract:
:Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the vertebrate brain. GABA activates both ionotropic (GABA(A)) and metabotropic (GABA(B)) receptors in mammals. Whether non-mammalian vertebrates possess receptors with similar characteristics is not well understood. We used a mammalian GABA(B)-specific antagonist to determine the pharmacology of putative receptors in the brain of an anuran amphibian, the male bullfrog (Rana catesbeiana). Receptor binding assays with the antagonist [(3)H]CGP54626 revealed a single class of high affinity binding sites (with a K(D) of 2.97 nM and a B(max) of 2619 fmol/mg protein). Binding was time- and temperature-dependent, saturable and specific. Specific binding of [(3)H]CGP54626 was inhibited by several mammalian GABA(B) receptor agonists and antagonists. The rank order potency of agonists was: GABA = SKF97541 > (R)-Baclofen > 3-APPA. The rank order for antagonists was: CGP54626 = CGP55845 > CGP52432 > CGP35348. The GABA(A) receptor ligands muscimol and SR95531 had very low affinity for [(3)H]CGP54626 binding sites, while bicuculline compounds had no affinity. Binding of GABA was positively modulated by CGP7930. Taurine did not allosterically modulate GABA binding but did inhibit [(3)H]CGP54626 binding in a linear fashion. Bullfrog brain thus possesses binding sites with significant similarity to mammalian GABA(B) receptors. These receptors differ from mammalian receptors, however, in dissociation kinetics, ligand specificity and allosteric modulation.
journal_name
Brain Resjournal_title
Brain researchauthors
Asay MJ,Boyd SKdoi
10.1016/j.brainres.2006.03.008subject
Has Abstractpub_date
2006-06-13 00:00:00pages
76-85issue
1eissn
0006-8993issn
1872-6240pii
S0006-8993(06)00668-8journal_volume
1094pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Colchicine was injected into the region of the organum vasculosum lamina terminalis/anterior region of the third cerebral ventricle (OVLT/AV3V) to produce a temporary disruption of the nervous connections of this area and the rest of the anterior hypothalamus, but to maintain the vascular connections intact. Rats were...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91648-9
更新日期:1987-12-29 00:00:00
abstract::Gonadotrophin-releasing hormone (GnRH) neurons do not express the leptin receptor (OB-R) in the medial preoptic area (MPOA) and the medial basal hypothalamus (MBH). We assessed whether the effect of leptin on the secretion of luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin (PRL) in proestrus...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.02.001
更新日期:2015-04-16 00:00:00
abstract::In urethane-anesthetized cats, ordinary and partial coherence values relating the 10-Hz rhythmic discharges of sympathetic nerves located on opposite sides of the body were not significantly affected by midsagittal section of the spinal cord from the seventh cervical through eighth thoracic or first cervical through e...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)01355-l
更新日期:1995-01-30 00:00:00
abstract::Spectrally opponent processes of ON-type retinal ganglion cells and the blue preference behavior were identified by parallel physiological and behavioral experiments in Rana pipiens. Spectral opponency of retinal ON-units was measured by recording from optic nerve terminals in the anterior thalamus, while the retina w...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90888-x
更新日期:1981-04-06 00:00:00
abstract::The distribution of antidiabetic sulfonylurea [( 3H]glibenclamide) binding sites is heterogeneous in rat brain. Pyramidal and extrapyramidal motor system contain the highest densities of sites, particularly in the substantia nigra and in the globus pallidus. Only low levels are present in the hypothalamic nuclei and t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91288-2
更新日期:1989-05-01 00:00:00
abstract::The hypothalamic angiotensin II (Ang II) system plays an important role in pituitary hormone release. Little is known about this system in the mouse brain. We studied the distribution of angiotensin-converting-enzyme (ACE), Ang II, Ang II receptor subtypes, and vasopressin in the hypothalamus of adult male mice. Autor...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00220-5
更新日期:1997-05-23 00:00:00
abstract::The trisomy-16 mouse is considered to be a model of human trisomy-21 (Down syndrome). We have examined the electrical membrane properties of cultured dorsal root ganglion (DRG) neurons from normal and trisomy-16 fetuses. Trisomy-16 neurons had significantly accelerated rates of action potential depolarization and repo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90678-1
更新日期:1989-04-17 00:00:00
abstract::The retrograde transport of fluorescent substances was used in order to investigate divergent axon collaterals of neurons in the nucleus prepositus hypoglossi (Ph). Fast blue (FB) was injected into the flocculus, paraflocculus and/or the vermis, while nuclear yellow (NY) was injected into the oculomotor nucleus alone ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90150-0
更新日期:1982-08-19 00:00:00
abstract::Mitochondrial division inhibitor (mdivi-1), a selective inhibitor of a mitochondrial fission protein dynamin-related protein 1 (Drp1), has been shown to exert protective effects in heart and cerebral ischemia-reperfusion models. The present study was designed to investigate the beneficial effects of mdivi-1 against sp...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.03.033
更新日期:2015-09-04 00:00:00
abstract::Spinal nociceptive neural circuits undergo considerable changes during the postnatal period. This study showed that neonatal rats exhibited earlier upregulation and faster recovery of spinal preprodynorphin (PPD) mRNA than did the adults during complete Freund's adjuvant (CFA)-induced peripheral inflammation. These da...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.01.039
更新日期:2005-03-21 00:00:00
abstract::In animals with retinal degeneration, the presence of activated microglial cells in the outer retina during the early stages of injury suggests that they may be involved in the ensuing photoreceptor cell death. In the following study, we investigated the effects of rat retina-derived microglial cells on a photorecepto...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01625-x
更新日期:1999-07-31 00:00:00
abstract::Male Fischer-344 rats were injected i.p. with diethyldithiocarbamate or dithizone 15 min after kainic acid (KA), s.c. Diethyldithiocarbamate and dithizone reduced both the number of wet dog shakes and the latency to onset of seizures induced by KA. Moreover, they increased the severity of seizures. These compounds may...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)91273-j
更新日期:1990-01-08 00:00:00
abstract::Acetylcholinesterase (AChE) histochemical staining occurred in neurons of the dentate gyrus at the day of birth and steadily increased in intensity and distribution during the first 3 postnatal weeks until the adult pattern was reached. Granule cells failed to display AChE staining; however, the somata of most non-pri...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(87)90072-1
更新日期:1987-11-01 00:00:00
abstract::The effects of glucagon injections (25, 100 and 200 ng) into the paraventricular nucleus of the hypothalamus (PVN) were investigated in an open-circuit calorimeter. Wistar rats were tested, with no food available during the tests. High doses of glucagon (100 and 200 ng) produced small and short-lasting increases in en...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90663-8
更新日期:1993-12-10 00:00:00
abstract::Experiments were carried out to determine if stimuli which augment preganglionic nerve activity to sympathetic neurons, and thereby cause trans-synaptic induction, increase the retrograde transport of nerve growth factor (NGF). It was found that nerve activity had no effect on retrograde transport of [125I]NGF. It was...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(79)90701-7
更新日期:1979-12-14 00:00:00
abstract::Veratridine-induced depolarization caused a large increase in Ca uptake in the rat hippocampal slice (30.2 vs. 9.0 nM/mg dry weight). This uptake was reduced to 18.4 nM/mg when veratridine was combined with anoxia. When compared with veratridine exposure alone, the combination of anoxia and veratridine increased intra...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91547-4
更新日期:1994-01-07 00:00:00
abstract::Nocistatin, a product of the same precursor as nociceptin/orphanin FQ (N/OFQ), has been shown to antagonize effects of N/OFQ. N/OFQ stimulates feeding, most probably by inhibiting activation of neurons containing oxytocin (OT) and vasopressin (VP), peptides considered as satiety factors, and implicated in the developm...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02535-x
更新日期:2000-07-28 00:00:00
abstract::MicroRNAs (miRNAs) are short, non-coding RNAs that negatively regulate target gene expression, and play an important role in cerebral ischemic injury. MiR-146a has been reported to be highly related to cell invasion, metastasis, immunity, inflammation and apoptosis. Previous studies have indicated that miR-146a can ei...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2016.07.034
更新日期:2016-10-01 00:00:00
abstract::Sleep spindles may be involved in synaptic plasticity. Learning-dependent increases in spindles have been observed in both humans and rats. In humans, the innate (i.e., baseline) number of spindles correlate with measures of academic potential such as Intelligence Quotient (IQ) tests. The present study investigated if...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.01.026
更新日期:2010-03-10 00:00:00
abstract::This research examined the electrophysiological, behavioral and neuroanatomical changes following an injection of kainic acid (KA) which destroyed most of the CA3 pyramidal cells in the hippocampus. For 5 days following the injection of KA, the evoked potential (EP) in the CA1 pyramidal cells produced by stimulation o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90677-2
更新日期:1984-10-29 00:00:00
abstract::Phenylethanolamine N-methyltransferase (PNMT) activity was measured in adrenal glands and medulla oblongata from 4 inbred rat strains, Fischer 344, Buffalo, Lewis and Sprague-Dawley rats. Adrenal enzyme activity was markedly different among the strains with the highest in Fischer, followed by Sprague-Dawley, Lewis and...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)91476-9
更新日期:1986-04-30 00:00:00
abstract::The cataleptic activity of morphine and methadone was markedly potentiated in frontally decorticated rats with no apparent changes in the onset or duration of action. Enhancement of the opioid cataleptic response was not due to changes in the availability of the drugs in the brain. The potentiation of methadone-induce...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91028-1
更新日期:1988-05-24 00:00:00
abstract::The present study investigated the hypothesis that social isolation increases aggression by increasing the number of V1a vasopressin receptors in the anterior hypothalamus (AH). Male hamsters were randomly assigned to a group that was allowed to interact with a small nonaggressive hamster three times each week for 3 w...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.12.081
更新日期:2006-02-16 00:00:00
abstract::Spontaneous alternation which is disrupted by lesion of septal dopaminergic (DA) afferents was chosen as a behavioral marker for the study of functional interactions between DA and noradrenergic (NA) innervation of the lateral septum. Three groups of rats were studied: a solvent group which received only vehicle injec...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90669-m
更新日期:1991-06-21 00:00:00
abstract::Sterol regulatory element binding protein (SREBP)-1 is a membrane-bound transcription factor that regulates the expression of several genes involved in cellular fatty acid synthesis in the peripheral tissues, including liver. Although SREBP-1 is expressed in brain, little is known about its function. The aim of the pr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.01.081
更新日期:2006-04-07 00:00:00
abstract::Evolutionary selection pressures have been one of the factors proposed to underlie sex differences in inhibitory control. Consequently, inhibitory control may vary as a function of the menstrual cycle and may be modulated by the stimuli being processed if these stimuli are related to reproductive success. We used func...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.05.061
更新日期:2008-08-11 00:00:00
abstract::The effects of diazepam, nitrazepam, clonazepam, and Ro5-4864 on transient (type I) and long-lasting (type II) calcium channels associated with low-affinity benzodiazepine receptors were investigated using the whole-cell patch-clamp technique. Clonazepam (100 microM), a specific agonist for the central-type benzodiaze...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90991-u
更新日期:1993-03-26 00:00:00
abstract::A pair of neurons exhibiting postinhibitory rebound, if connected through reciprocally inhibitory chemical synapses, will exhibit a stable pattern of alternating bursts. If two such oscillating pairs, of similar but not identical properties are connected by means of an electrical synapse and an inhibitory chemical syn...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90742-3
更新日期:1981-12-14 00:00:00
abstract::The effects of axotomy, chemical sympathectomy and preganglionic denervation on the expression of the neuropeptides, pituitary adenylate cyclase-activating peptide (PACAP), galanin (GAL), and the PACAP type 1 receptor in the rat superior cervical ganglion (SCG) were investigated by immunocytochemistry, in situ hybridi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00923-2
更新日期:1997-11-14 00:00:00
abstract::Aging is accompanied by an impairment of associative memory. The medial temporal lobe and fronto-striatal network, both involved in associative memory, are known to decline functionally and structurally with age, leading to the so-called associative binding deficit and the resource deficit. Because the MTL and fronto-...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.12.011
更新日期:2010-02-22 00:00:00