Abstract：
:Several human tumour cell lines were screened for secretion of proenkephalin-derived peptides with an antiserum directed to its N-terminus, Met-enkephalin-Arg6,Phe7 and for proopiomelanocortin-derived peptides with an antiserum to beta-endorphin. The neuroblastoma SK-N-MC cell line secreted Met-enkephalin-Arg6,Phe7-immunoreactive peptides in relatively high amounts into the culture medium, although processing was not complete and there was no evidence for free Met-enkephalin-Arg6,Phe7. Gene expression was confirmed by the presence of proenkephalin mRNA and proenkephalin-derived polypeptides in extracts of the SK-N-MC cells and also in the neuroblastoma SH-SY5Y cell line. In the latter cells, however, the expression was approximately 3 times lower, there was less processing of proenkephalin and no evidence for secretion.
journal_name
Brain Resjournal_title
Brain researchauthors
Folkesson R,Monstein HJ,Geijer T,Påhlman S,Nilsson K,Terenius Ldoi
10.1016/0169-328x(88)90060-5subject
Has Abstractpub_date
1988-04-01 00:00:00pages
147-54issue
2eissn
0006-8993issn
1872-6240journal_volume
427pub_type
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