Abstract：
:Factor H (FH) is the predominant soluble inhibitor of the complement system. With a concentration of 200-800 microg/ml in human and rat plasma it acts as a cofactor for the soluble factor I (FI)-mediated cleavage of the component C3b to iC3b. Furthermore it competes with factor B for binding to C3b and C3(H2O) and promotes the dissociation of the C3bBb complex. FH is a monomer of about 155 kDa which comprises 20 short consensus repeats (SCR), each of which is composed of approximately 60 amino acid (aa) residues. Two functional fragments of FH comprising the SCR1-4 or SCR1-7 were generated using either the Baculovirus system or stably transfected human embryonal kidney cells, respectively. These fragments, as well as FH purified from rat serum, were first analyzed for their relative molecular weights (Mr) using non-reducing or reducing SDS-PAGE. The Mr of the FH variants differed by about 20% depending on the experimental conditions employed. Only the Mr of proteins separated under reducing conditions were in accordance with the MW calculated from the aa sequence. Analyses of the glycosylation patterns using PAS-staining showed a lack of staining of the recombinant variants (SCR1-4 and SCR1-7) in contrast to FH(SCR1-20) from serum. Using a complement hemolysis assay (CH50-assay) all three variants exhibited a molar complement inhibitory activity of FH(1-20)/FH(1-7)/FH(1-4) of about 3/1/1. These data support the postulated model of FH bearing three binding sites for its ligand C3b, from which one is located in the SCR1-4, whereas the other two are located in the SCR8-20.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Demberg T,Heine I,Götze O,Altermann WW,Seliger B,Schlaf Gdoi
10.14670/HH-21.93subject
Has Abstractpub_date
2006-01-01 00:00:00pages
93-102issue
1eissn
0213-3911issn
1699-5848journal_volume
21pub_type
杂志文章,评审abstract：:We review here the 14 members of the Monocarboxylate transporter family (MCTs), their relationship based on sequence homology. The range of substrates transported by different members of this family extends from the standard monocarboxylate metabolites, lactic and pyruvic acids, to aromatic amino acids and thyroid hor...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-24.243
更新日期：2009-02-01 00:00:00
abstract：:Detailed characterization of the subdermal model is a significant tool for better understanding of calcification mechanisms occurring in heart valves. In previous ultrastructural investigation on six-week-implantated aortic valve leaflets, modified pre-embedding glutaraldehyde-cuprolinic-blue reactions (GA-CB) enabled...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-22.261
更新日期：2007-03-01 00:00:00
abstract：:Melanocytes characterized by their tyrosinase activity, melanosomes and dendrites locate in the basal layer of epidermis and hair bulb in the skin of mice. Melanocytes differentiate from undifferentiated melanoblasts derived from embryonic neural crest. Melanocyte-stimulating hormone plays an important role in the reg...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：:Neutrophil extracellular traps (NETs) are innate immune systems against invading pathogens. NETs are characterized as released DNA mixed with cytoplasmic antimicrobial proteins such as myeloperoxidase, proteinase3 and neutrophil elastase. While NETs are thought to have an important role in host defense, recent work ha...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-11-816
更新日期：2017-03-01 00:00:00
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journal_title：Histology and histopathology
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doi：
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abstract：:The study aimed at determining levels of metallothionein (MT) and Ki-67 antigen expression in adenocarcinomas of large intestine and examining relation of the expression levels with various clinical and pathological variables. The studies were performed on 81 cases of large intestine adenocarcinoma. Using immunocytoch...
journal_title：Histology and histopathology
pub_type： 临床试验,杂志文章
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更新日期：2003-04-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
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更新日期：2014-10-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:TNFalpha exerts apoptosis throughout an intracellular transduction pathway that involves the kinase proteins TRAF-2 (integration point of apoptotic and survival signals), ASK1 (pro-apoptotic protein), MEK-4 (p38 activator and metastasis suppressor gene), JNK (stress mitogen activated protein kinase) and the transcript...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
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更新日期：2008-10-01 00:00:00
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journal_title：Histology and histopathology
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doi：
更新日期：1988-04-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-18.695
更新日期：2003-07-01 00:00:00
abstract：:To clarify the role of REG IV, a new member of the regenerating gene (REG) family, in tumorigenesis and progression of colorectal carcinoma (CRC), 320 CRC specimens, 123 corresponding adjacent non-cancerous mucosa (ANCMs), 46 corresponding non-adjacent non-cancerous mucosa (NANCMs) and 86 adenomas were investigated im...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-25.473
更新日期：2010-04-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：BACKGROUND:Little is known regarding the role of redox balance regulators in metastatic melanomas, but there is some evidence for a link between epithelial-to-mesenchymal transition (EMT) and cellular redox status. METHODS:We compared the immunohistochemical expression of nuclear factor erythroid-2-related factor 2 (N...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-778
更新日期：2017-02-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
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更新日期：2004-04-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-640
更新日期：2015-12-01 00:00:00
abstract：:Thrombospondin-1 (TSP-1) is a matrix protein implicated in mechanisms of wound healing. TSP-1 contains the sequence cysteine-serine-valine-threonine-cysteine-glycine (CSVTCG) that has been shown to function primarily as a cell adhesion domain. Our laboratory has isolated a novel receptor specific for the CSVTCG adhesi...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-13.967
更新日期：1998-10-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-590
更新日期：2015-07-01 00:00:00
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journal_title：Histology and histopathology
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doi：
更新日期：2013-04-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
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更新日期：2007-10-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-811
更新日期：2017-05-01 00:00:00
abstract：:Primary perivascular epithelioid cell tumor (PEComa) of the liver is a very rare example of an emerging family of hepatic PEC tumors. Only few cases have been described so far. We report the case of a large but benign hepatic PEComa in a 53-year-old man without signs of tuberous sclerosis. In contrast to recently desc...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-23.1185
更新日期：2008-10-01 00:00:00
abstract：:In this paper we report the first complete mapping of the pituitary in a tuna species. The various different adenohypophysis cell types of the bluefin tuna, Thunnus thynnus L. have been identified and located using different antisera against mammalian and piscine hormones and various histochemical techniques: PAS, Alc...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-16.443
更新日期：2001-04-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The effects of cadmium exposure on the protein secretory functions of cultured neonatal rat hepatocytes were analyzed by both two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and electron microscopy. [35S]Methionine-labelled protein secretion was significantly depressed by cadmium exposure in a dose-depend...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:High mobility group box 1 protein (HMGB1) is a non-histone chromosomal protein which is highly conserved, ubiquitous, and widely distributed. HMGB1 has multiple functions in the nucleus, including the maintenance of nucleosome structure, the regulation of gene transcription, and involvement in DNA recombination. HMBG1...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-29.235
更新日期：2014-02-01 00:00:00
