Hepatic response to chronic hypoxia in experimental rat model through HIF-1 alpha, activator protein-1 and NF-kappa B.

Abstract:

:Chronic liver diseases are commonly associated with tissue hypoxia that may cause inflammation, oxidative stress, liver cell injury and increased nuclear transcriptional regulation. The hepatic response to chronic hypoxia at the molecular level has not yet been clearly understood until now. The aim of this study is to investigate whether nuclear transcription factors [hypoxia-inducible factor-1 (HIF-1α), activator protein-1 (AP-1), nuclear factor-kappa B (NF-κB)] exhibit activity changes during hepatic response to chronic hypoxia. Blood and liver samples were collected from adult Sprague-Dawley rats living in atmospheric air or 10% oxygen for four weeks. Levels of serum alanine aminotransferase (ALT), 8-isoprostane and nitrotyrosine were measured. The activities of nuclear transcription factors and the expression of downstream genes (iNOS, eNOS, ET-1 and VEGF) were measured using RT-PCR, Western blotting and Gel shift analysis. Results showed that serum ALT level, 8-isoprostane level and formation of nitrotyrosine were within normal range at all time-points. In the hypoxic liver, DNA-binding activities of HIF-1α, NF-κB and AP-1 increased significantly. Expression levels of iNOS, VEGF and ET-1 progressively increased from day 7 to day 28. eNOS was also elevated in the hypoxic liver. In conclusion, our study suggests that increased activity of HIF-1α, AP-1 and NF-κB may partly play a significant role in the hepatic response to oxidative stress and liver injury under chronic hypoxia. The increased expression of VEGF, ET-1, iNOS and eNOS may be partly due to the compensatory mechanism in the vascular beds of the liver in response to chronic hypoxia.

journal_name

Histol Histopathol

authors

Lau TY,Xiao J,Liong EC,Liao L,Leung TM,Nanji AA,Fung ML,Tipoe GL

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

463-71

issue

4

eissn

0213-3911

issn

1699-5848

pii

HH-11-247

journal_volume

28

pub_type

杂志文章
  • Modulation of pulmonary neuroendocrine cells in idiopathic interstitial pneumonia.

    abstract::In order to reveal modulation of the number of pulmonary neuroendocrine cells (PNEC) in interstitial lung diseases and to clarify significance of cell proliferation activity in occurrence of PNEC, we counted airway PNEC of the patients of idiopathic interstitial pneumonia, secondary interstitial pneumonia and control ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-17.1121

    authors: Ito T,Ogura T,Ogawa N,Udaka N,Hayashi H,Inayama Y,Yazawa T,Kitamura H

    更新日期:2002-10-01 00:00:00

  • Perspectives of SLIT/ROBO signaling in placental angiogenesis.

    abstract::A novel family of evolutionally conserved neuronal guidance cues, including ligands (i.e., Slit, netrin, epherin, and semaphorin) and their corresponding receptors (i.e., Robo, DCC/Unc5, Eph and plexin/ neuropilin), has been identified to play a crucial role in axon pathfinding and branching as well as neuronal cell m...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-25.1181

    authors: Liao WX,Wing DA,Geng JG,Chen DB

    更新日期:2010-09-01 00:00:00

  • Cadmium toxicity on cultured neonatal rat hepatocytes: biochemical and ultrastructural analyses.

    abstract::The effects of cadmium exposure on the protein secretory functions of cultured neonatal rat hepatocytes were analyzed by both two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and electron microscopy. [35S]Methionine-labelled protein secretion was significantly depressed by cadmium exposure in a dose-depend...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Hirano T,Ueda H,Kawahara A,Fujimoto S

    更新日期:1991-01-01 00:00:00

  • Effects of thyroid hormones on Leydig cells in the postnatal testis.

    abstract::Thyroid hormones (TH) stimulate oxidative metabolism in many tissues in the body, but testis is not one of them. Therefore, in this sense, testis is not considered as a target organ for TH. However, recent findings clearly show that TH have significant functions on the testis in general, and Leydig cells in particular...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-19.985

    authors: Mendis-Handagama SM,Ariyaratne HB

    更新日期:2004-07-01 00:00:00

  • Renal clear-cell carcinoma: an ultrastructural study on the junctional complexes.

    abstract::Junctional complexes such as tight junctions, adherens junctions, and desmosomes play crucial roles in the structure and function of epithelial cells. These junctions are involved in increasing cell-cell contact and as well serve as signaling centers regulating multiple functions in epithelial cells. Carcinoma cell li...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-20.35

    authors: Kim G,Rajasekaran SA,Thomas G,Rosen EA,Landaw EM,Shintaku P,Lassman C,Said J,Rajasekaran AK

    更新日期:2005-01-01 00:00:00

  • Adhesion-dependent signalling and the initiation of haemostasis and thrombosis.

    abstract::Cell-cell and cell-extracellular matrix adhesion are critical aspects of platelet function, regulating interactions between circulating platelets in the bloodstream with the blood vessel wall. In haemostasis, platelets adhere to the subendothelial matrix of a damaged vessel, spread over the surface and recruit additio...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-13.837

    authors: Andrews RK,Berndt MC

    更新日期:1998-07-01 00:00:00

  • Histochemical aspects of the yolk-sac and digestive tract of larvae of the Senegal sole, Solea senegalensis (Kaup, 1858).

    abstract::Histochemical distribution of glycoproteins, carbohydrates and proteins rich in different amino acids were studied using histological and histochemical procedures, in Senegal sole, Solea senegalensis (Kaup, 1858) larvae from hatching until day 15. Glycogen, proteins and glycoproteins were detected in the yolk-sac of t...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Sarasquete C,González de Canales ML,Arellano JM,Muñoz-Cueto JA,Ribeiro L,Dinis MT

    更新日期:1996-10-01 00:00:00

  • Review of papillary renal cell carcinoma with focus on clinical and pathobiological aspects.

    abstract::Recent studies have shown that papillary renal cell carcinoma (RCC) is clinically and genotypically a distinct entity. Papillary RCCs account for about 10-15% of renal parenchymal neoplasms. Macroscopically, the cut surface is yellow or brown in color and large tumors frequently show cystic change. Hemorrhage and necr...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-18.487

    authors: Kuroda N,Toi M,Hiroi M,Enzan H

    更新日期:2003-04-01 00:00:00

  • Collagen-platelet interaction: platelet non-integrin receptors.

    abstract::Platelet-collagen interaction is a complex event that involves ligand-receptor interaction. There are many adhesive non-integrin receptors for platelets to interact with various types of collagens. These non-integrin receptors also serve as signal transducers both from the outside of platelets to the inside and possib...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-14.579

    authors: Chiang TM

    更新日期:1999-04-01 00:00:00

  • Recent development of in vivo cryotechnique to cryobiopsy for living animals.

    abstract::Various microscopic methods have been used to analyze the morphology and molecular distribution of cells and tissues. Using conventional procedures, however, ischemic or anoxic artifacts are inevitably caused by tissue-resection or perfusion-fixation. The in vivo cryotechnique (IVCT) was developed to overcome these pr...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-22.1281

    authors: Ohno N,Terada N,Saitoh S,Zhou H,Fujii Y,Ohno S

    更新日期:2007-11-01 00:00:00

  • Telocytes form networks in normal cardiac tissues.

    abstract::Telocytes (TC) are a class of interstitial cells present in heart. Their characteristic feature is the presence of extremely long and thin prolongations (called telopodes). Therefore, we were interested to see whether or not TCs form networks in normal cardiac tissues, as previously suggested. Autopsy samples of card...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-27.807

    authors: Rusu MC,Pop F,Hostiuc S,Curca GC,Jianu AM,Paduraru D

    更新日期:2012-06-01 00:00:00

  • Tumour-associated tissue eosinophilia (TATE) in oral squamous cell carcinoma: a comprehensive review.

    abstract::Oral squamous cell carcinoma is the most common head and neck malignancy, characterised by high invasive capacity, lymph node metastasis, and high recurrence rate. Among the morphological features of oral cancer, the tumour-associated tissue eosinophilia has gained growing interest in the last years. Eosinophils are a...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-18-250

    authors: Mascitti M,Togni L,Rubini C,Troiano G,Lo Muzio L,Santarelli A

    更新日期:2020-09-28 00:00:00

  • Hydroxy group requirement for halofuginone-dependent inhibition of muscle fibrosis and improvement of histopathology in the mdx mouse model for Duchenne muscular dystrophy.

    abstract::In Duchenne muscular dystrophy (DMD), the progressive loss of muscle and its ability to function is associated with significant fibrosis, representing the major disease complication in patients. Halofuginone, a halogenated analog of the naturally occurring febrifugine, has been shown to prevent fibrosis in various ani...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-18-083

    authors: Wellner G,Mordechay S,Evans P,Genin O,Pines M,Halevy O

    更新日期:2019-07-01 00:00:00

  • Current understanding of macrophage type 1 cytokine responses during intracellular infections.

    abstract::Macrophages are important effector cells in cell-mediated immunity against intracellular infection. Among cytokines that macrophages are able to release are IL-12 and TNF alpha. IL-12 is a critical linker between the innate and adaptive cell-mediated immunity, capable of Th1 differentiation and IFN gamma release by T ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-15.199

    authors: Xing Z

    更新日期:2000-01-01 00:00:00

  • Enhanced CD24 expression in endometrial carcinoma and its expression pattern in normal and hyperplastic endometrium.

    abstract::CD24 is known to be an important diagnostic and prognostic marker of several major cancers affecting females. We aimed to determine CD24 expression in normal, hyperplastic, and carcinomatous endometrium and its correlation with estrogen and progesterone receptor expression. A total of 271 cases including 62 normal/atr...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-24.309

    authors: Kim KH,Choi JS,Kim JM,Choi YL,Shin YK,Lee HC,Seong IO,Kim BK,Chae SW,Kim SH

    更新日期:2009-03-01 00:00:00

  • Metalloproteinase expression by control and telomerase immortalized human endometrial endothelial cells.

    abstract::Vascular endothelial cells play a critical role in the maintenance of endometrial homeostasis. Indeed many pathological conditions causing abnormal endometrial bleeding including progestin only contraception, hormone replacement therapy, endometrial polyps, myomas, hyperplasia and cancer are associated with aberrant a...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-20.719

    authors: Krikun G,Mor G,Huang J,Schatz F,Lockwood CJ

    更新日期:2005-07-01 00:00:00

  • Anti-apoptotic activity in deep pelvic endometriosis.

    abstract::Since endometriosis is a proliferative disease we evaluated the presence of anti-apoptotic factor (Bcl-2) and pro-apoptotic factor (Bax) in deep pelvic endometriosis. A Cross-sectional observational study was performed at Santa Casa de Misericórdia de São Paulo, São Paulo, Brazil. Forty women aged 26 to 46 years with ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-29.1129

    authors: Abdalla Ribeiro HS,Galvāo MA,Aoki T,Aldrighi JM,Ribeiro PA

    更新日期:2014-09-01 00:00:00

  • Macrophage populations and expressions of regulatory proinflammatory factors in the rat meninx under lipopolysaccharide treatment in vivo and in vitro.

    abstract::Macrophages play important roles in host defense mechanisms. In the brain, besides microglial cells, meningeal macrophages are present. However, the pathobiological characteristics of meningeal macrophages in rats remain to be investigated. In normal meninx, immunohistochemically, macrophages reacting to CD163 (macrop...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-24.13

    authors: Yamate J,Ishimine S,Izawa T,Kumagai D,Kuwamura M

    更新日期:2009-01-01 00:00:00

  • Selective immunolabeling of early gestational cytotrophoblast and its neoplastic counterpart by the monoclonal antibody Ber-EP4.

    abstract::This report presents preliminary observations on the distribution of an epithelial surface antigen in human trophoblastic cells studied with the monoclonal antibody Ber-EP4 by means of an immunoperoxidase technique. Three cases of gestational choriocarcinoma and 20 cases of both villous and extravillous trophoblast, i...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Babury RA,Moscovic EA

    更新日期:1993-04-01 00:00:00

  • Effects of orexins A and B on the secretory and proliferative activity of immature and regenerating rat adrenal glands.

    abstract::Orexins A and B are two hypothalamic peptides, involved in the central regulation of feeding, which act through two receptor subtypes, named OX1R and OX2R. OX1R is selective for orexin-A, and OX2R binds both orexins. We have investigated the effects of three subcutaneous injections of 10 nmol/kg body weight of orexins...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-16.713

    authors: Malendowicz LK,Jedrzejczak N,Belloni AS,Trejter M,Hochól A,Nussdorfer GG

    更新日期:2001-07-01 00:00:00

  • Topoisomerase 1A, HER/2neu and Ki67 expression in paired primary and relapse ovarian cancer tissue samples.

    abstract::In the present study we examined prognostic value of immunohistochemical estimation of topoisomerase 1A (TOP 1A) and HER-2/neu expression in ovarian cancers treated with platinum-based drugs but not with topotecan and the relation between expression of these proteins on the one hand and intensity of proliferation (Ki6...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-21.713

    authors: Surowiak P,Materna V,Kaplenko I,Spaczynski M,Dietel M,Lage H,Zabel M

    更新日期:2006-07-01 00:00:00

  • The glomerular distribution of laminin and fibronectin in glomerulonephritis.

    abstract::Laminin (LAM) and fibronectin (FI) are regarded as major components of the glomerular extracellular matrix. The aim of this study was to define the distribution of LAM and FI in primary glomerulonephritis (GN) and GN of systemic lupus erythematosus (SLE) and to correlate the type of glomerular disorders with possible ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Nakapoulou I,Stefanaki K,Zeis PM,Papadakis J,Boletis J,Vosnidis G,Davaris P

    更新日期:1993-07-01 00:00:00

  • Morphology of epiphyseal apparatus of a ranid frog (Rana Esculenta)

    abstract::Morphological, histochemical and ultrastructural investigations on epiphyseal apparatus of Rana Esculenta were made. The most important findings were the following: 1) metaphyseal cartilage is localized inside proximal diaphyseal compact bone as a plug; 2) metaphyseal cartilage do not reduce in thickness during ageing...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Dell'Orbo C,Gioglio L,Quacci D

    更新日期:1992-04-01 00:00:00

  • Nitric oxide synthase in the pineal gland.

    abstract::The recent discovery of nitric oxide (NO) as a biological messenger molecule with unique characteristics has opened a new field in pineal research. This free radical gas is synthesized by the enzyme nitric oxide synthase (NOS) from L-arginine. The activation of adrenoreceptors in the membrane of the pinealocytes media...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:

    authors: López-Figueroa MO,Møller M

    更新日期:1996-10-01 00:00:00

  • Glucocorticoid receptor changes its cellular location with breast cancer development.

    abstract::Glucocorticoids play a major role in attenuation of the inflammatory response and they are useful in the primary combination chemotherapy of breast cancer, since in vitro studies have demonstrated an antiproliferative effect in human breast cancer cells. In contrast, it was recently shown that glucocorticoids protect ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-23.77

    authors: Conde I,Paniagua R,Fraile B,Lucio J,Arenas MI

    更新日期:2008-01-01 00:00:00

  • Liver growth factor antifibrotic activity in vivo is associated with a decrease in activation of hepatic stellate cells.

    abstract::The antifibrotic activity of Liver Growth Factor (LGF), a liver mitogen, was previously demonstrated in several models of rat liver fibrosis and even in extrahepatic sites, such as carotid artery in hypertensive rats and rat CdCl2-induced lung fibrosis. In the present study, we have attempted to examine in depth its m...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-24.473

    authors: Díaz-Gil JJ,García-Monzón C,Rúa C,Martín-Sanz P,Cereceda RM,Miquilena-Colina ME,Machín C,Fernández-Martínez A,García-Cañero R

    更新日期:2009-04-01 00:00:00

  • Histopathological changes in the islets of Langerhans in hamsters infected with the 139H strain of scrapie: semi-thin section study.

    abstract::Using histopathological analysis of semi-thin sections stained with toluidine blue, we observed profound pathological changes in the islets of Langerhans of hamsters infected with the scrapie agent (strain 139H). These included cytoplasmic vesicles, nuclear swelling, and vacuolization in the islet cells. Two types of ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Ye X,Carp RI

    更新日期:1996-01-01 00:00:00

  • Physiopathologic dynamics of vesicle traffic in astrocytes.

    abstract::The view of how astrocytes, a type of glial cells, contribute to the functioning of the central nervous system (CNS) has changed greatly in the last decade. Although glial cells outnumber neurons in the mammalian brain, it was considered for over a century that they played a subservient role to neurons. This view chan...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-26.277

    authors: Potokar M,Stenovec M,Kreft M,Gabrijel M,Zorec R

    更新日期:2011-02-01 00:00:00

  • Decalcification by perfusion. A new method for rapid softening of temporal bones.

    abstract::We describe a new technique, decalcification by perfusion, for the softening of bony tissue. The blood circulatory system was perfused in 16 rats via a cannula through the left heart ventricle with a fixative followed by New Decalc (an acidic demineralizer) for 30-240 minutes. Perfusion decalcification for 120 minutes...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Nilsson M,Hellström S,Albiin N

    更新日期:1991-07-01 00:00:00

  • The combined effect of honey and olive oil against methotrexate mediated hepatotoxicity in rats: A biochemical, histological and immunohistological study.

    abstract:BACKGROUND:Honey and olive oil are natural products that have high nutritional values, and therapeutic properties. Cytotoxic drugs, like methotrexate (MTX) are used to treat malignancies in tumour cells; however, these drugs also have serious side effects that could threaten the patient's life. AIM:To evaluate the pot...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-18-126

    authors: Alturkistani HA,Abuzinadah OAH,Kelany AM,Abd El-Aziz GS,Alrafiah AR

    更新日期:2019-12-01 00:00:00