Abstract:
:Detailed characterization of the subdermal model is a significant tool for better understanding of calcification mechanisms occurring in heart valves. In previous ultrastructural investigation on six-week-implantated aortic valve leaflets, modified pre-embedding glutaraldehyde-cuprolinic-blue reactions (GA-CB) enabled sample decalcification with concurrent retention/staining of lipid-containing polyanionic material, which lined cells and cell-derived matrix-vesicle-like bodies (phthalocyanin-positive layers: PPLs) co-localizing with the earliest apatite nucleation sites. Additional post-embedding silver staining (GA-CB-S) revealed PPLs to contain calcium-binding sites. This investigation concerns valve leaflets subjected to shorter implantation times to shed light on the modifications associated with PPLs generation and calcification onset/progression. Spectrometric estimations revealed time-dependent calcium increase, for unreacted samples, and copper modifications indicating an increase in acidic, non-glycanic material, for GA-CB-reacted samples. Two-day-implant thin sections showed emission and subsequent reabsorption of lamellipodium-like protrusions by cells, originating ECM-containing vacuoles, and/or degeneration stages characterized by the appearance of GA-CB-S-reactive, organule-derived dense bodies and progressive dissolution of all cell membranes. In one-week-implants, the first PPL-lined cells were found to co-exist with cells where GA-CB-S-reactive material accumulated, or exudated towards their edges, or outcropped at the ECM milieu, so acquiring PPL features. PPL-derived material was observed increasingly to affect the ECM on thin sections of one-week- to six-week-implants. These results show an endogenous source for PPLs and reveal that a peculiar cascade of cell degenerative steps is associated with valve mineralization in the subdermal model, providing new useful parameters for more reliable comparison of this experimental calcification process versus the physiological and pathological processes.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Ortolani F,Bonetti A,Tubaro F,Petrelli L,Contin M,Nori SL,Spina M,Marchini Mdoi
10.14670/HH-22.261subject
Has Abstractpub_date
2007-03-01 00:00:00pages
261-72issue
3eissn
0213-3911issn
1699-5848journal_volume
22pub_type
杂志文章abstract::Hereditary hemochromatosis (HH) is an inherited iron overload disorder characterized by normal iron-driven erythropoiesis and abnormal iron metabolism, leading to excess iron deposited in parenchymal cells of liver, heart, and endocrine glands. Iron hormone, hepcidin, plays a critical role in iron homeostasis through ...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-11-762
更新日期:2016-08-01 00:00:00
abstract::The three-dimensional structure of the pancreatic ductular system (from the intercalated duct to the intercellular secretory canaliculus) is controversial and unclear. The aim of this study is to reveal the three-dimensional structure of the pancreatic ductular sysytem at the onset of pancreatitis. One day following r...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-17.107
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::The integrity of the thymus during early life is necessary for a proper maturation of the neuroendocrine system, including the adrenal axis. The thymic metallopeptide thymulin seems to be a central physiologic mediator of thymus-pituitary communication. Furthermore, neonatal thymulin gene therapy has been shown to pre...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-26.471
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abstract::Recent studies have shown that papillary renal cell carcinoma (RCC) is clinically and genotypically a distinct entity. Papillary RCCs account for about 10-15% of renal parenchymal neoplasms. Macroscopically, the cut surface is yellow or brown in color and large tumors frequently show cystic change. Hemorrhage and necr...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-18.487
更新日期:2003-04-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-16.239
更新日期:2001-01-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-18.597
更新日期:2003-04-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-11-792
更新日期:2016-11-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-11-992
更新日期:2018-09-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 临床试验,杂志文章
doi:10.14670/HH-18.401
更新日期:2003-04-01 00:00:00
abstract::Aneurysmal subarachnoid hemorrhage (SAH) remains a serious cerebrovascular disease. Even if SAH patients survive the initial insults, delayed cerebral ischemia (DCI) may occur at 4 days or later post-SAH. DCI is characteristics of SAH, and is considered to develop by blood breakdown products and inflammatory reactions...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-18-253
更新日期:2020-09-30 00:00:00
abstract::Knee osteoarthritis (KOA) is the most common progressive joint disorder associated with disability in the world. As a chronic disease, KOA has multifactorial etiology. However, the poor self-healing ability of the articular cartilage due to its intrinsic tissue hypovascularity and hypocellularity seems to be directly ...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-11-955
更新日期:2018-07-01 00:00:00
abstract::Heterotopic ossification (HO) is a debilitating condition in which cartilage and bone forms in soft tissues such as muscle, tendon, and ligament causing immobility. This process is induced by inflammation associated with traumatic injury. In an extremely rare genetic disorder called fibrodysplasia ossificans progessiv...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-29.1281
更新日期:2014-10-01 00:00:00
abstract::Melanocytes characterized by their tyrosinase activity, melanosomes and dendrites locate in the basal layer of epidermis and hair bulb in the skin of mice. Melanocytes differentiate from undifferentiated melanoblasts derived from embryonic neural crest. Melanocyte-stimulating hormone plays an important role in the reg...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:
更新日期:1995-01-01 00:00:00
abstract::Androgen receptor (AR) interact with many pathways involved in bladder cancer development and progression. FUS (fused in liposarcoma), a multifunctional protein essential for different cellular processes, has been demonstrated as a key link between androgen receptor signaling and cell-cycle progression in prostate can...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-18-295
更新日期:2020-12-23 00:00:00
abstract::In the cutaneous nerves, unmyelinated nerve fibers outnumber the myelinated ones but are scarcely analyzed, especially at autopsy. This indifference toward the pathology of unmyelinated nerve fibers may be due to the necessity of electron microscopic analyses and, more importantly, the obscurity of pathological altera...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-15.313
更新日期:2000-01-01 00:00:00
abstract::Gliomas are the most common intracranial malignant tumors in humans, and high-grade gliomas in particular pose a unique challenge due to their propensity for proliferation and tissue invasion. Our understanding of glioma oncogenesis, proliferation, and invasion has been greatly advanced in the past 10 years as researc...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-18.207
更新日期:2003-01-01 00:00:00
abstract::Detailed histomorphometric analysis of human conjunctival biopsy specimens has convincingly demonstrated that tissue remodeling of the extracellular matrix (ECM) is an essential and dynamic process associated with conjunctival scarring in ocular cicatricial pemphigoid (OCP). The conjunctival scarring often eventually ...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-16.1203
更新日期:2001-10-01 00:00:00
abstract::The purpose of this investigation was to clarify the mechanisms of amyloid fibril formation in human lymph nodes. In our present study, amyloid deposition was observed diffusely in all compartments of the lymph nodes. The deposition form showed extremely characteristic findings in its morphological features. Namely, a...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1986-07-01 00:00:00
abstract::This study explores the possibility of a regulatory role for cytokine IL-10 in platelet aggregation as an active vascular repair mechanism. Endothelial cells from human umbilical cord vein were cultured in the presence of different IL-10 concentrations (0-100 ng/ml). Platelet-rich plasma was then added to these cultur...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-18.695
更新日期:2003-07-01 00:00:00
abstract:AIMS:The prognostic application of YES1 in epithelial ovarian cancer (EOC) is currently unclear. We aimed to investigate the expression of YES1 and its correlation with survival outcome in patients with EOC. METHODS:A retrospective study of patients diagnosed with EOC at the Cancer Center, Sun Yat-Sen University, Guan...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-18-203
更新日期:2020-07-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-20.455
更新日期:2005-04-01 00:00:00
abstract:BACKGROUND AND RESULTS:Embryos from diabetic mice exhibit several forms of neural tube defects, including non-closure of the neural tube. In the present study, embryos collected at embryonic day 11.5 from diabetic pregnancies displayed open neural tube with architectural disruption of the surrounding tissues. The perce...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-26.965
更新日期:2011-08-01 00:00:00
abstract::Neural stem cells are defined as clonogenic cells with self-renewal capacity and the ability to generate all neural lineages (multipotentiality). Cells with these characteristics have been isolated from the embryonic and adult central nervous system. Under specific conditions, these cells can differentiate into neuron...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-19.1261
更新日期:2004-10-01 00:00:00
abstract::Smads are signal transducers for the members of the TGF-beta superfamily. Of these Smads, Smad4 is essential for TGF-beta signaling. The purpose of this study was to elucidate Smad4 expression and localization in 65 gastric adenomas, 49 intestinal-type and 39 diffuse type of gastric adenocarcinomas (including 12 cases...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-20.543
更新日期:2005-04-01 00:00:00
abstract::Gp96 is the endoplasmic reticulum (ER)-resident molecular chaperone, which is involved in the correction of unfolded proteins, in the activation of proteasome-dependent ER-associated degradation of the misfolded proteins, and in activation of the protein translation that modulates polypeptide traffic into the ER. Furt...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-28.61
更新日期:2013-01-01 00:00:00
abstract::Orexins A and B are two hypothalamic peptides, involved in the central regulation of feeding, which act through two receptor subtypes, named OX1R and OX2R. OX1R is selective for orexin-A, and OX2R binds both orexins. We have investigated the effects of three subcutaneous injections of 10 nmol/kg body weight of orexins...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-16.713
更新日期:2001-07-01 00:00:00
abstract::Pulmonary fibrosis is a common response to various injuries to the lung. The resolution of a fibroproliferative response after lung injury is key to survival. Although there are various initiating factors or causes, the terminal stages are characterized by proliferation and progressive accumulation of connective tissu...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-19.867
更新日期:2004-07-01 00:00:00
abstract::This paper reports the histopathological findings in the eyes of a 26-year-old female patient diagnosed with systemic lupus erythematosus (SLE) with peripheral neuropathy. The patient had no significant ocular problems. She died of pneumonia after two years of suffering. The eyeballs were procured at autopsy and the r...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-20.373
更新日期:2005-04-01 00:00:00