Abstract:
:Denaturing high-performance liquid chromatography (DHPLC) is a relatively new technique, which utilizes heteroduplex formation between wild-type and mutated DNA strands to identify point mutations. Heteroduplex molecules are separated from homoduplex molecules by ion-pair, reverse-phase liquid chromatography on a special column matrix with partial heat denaturation of the DNA strands. In order to investigate the application of this method for point mutation detection in drug-resistant genes of Mycobacterium tuberculosis, katG, rpoB, embB, gyrA, pncA and rpsL genes, which are responsible for isoniazid, rifampicin, ethambutol, fluoroquinolone, pyrazinamide and streptomycin resistance, respectively, were detected by temperature-mediated DHPLC in 10 multidrug-resistant and 10 drug-susceptible clinical isolates. The DHPLC data were compared with those from a conventional MIC test. The results show that DHPLC is cost-effective with high capacity and accuracy, and is potentially useful for genotypic screening for mutations associated with anti-tuberculosis drug resistance.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Shi R,Otomo K,Yamada H,Tatsumi T,Sugawara Idoi
10.1016/j.micinf.2005.06.008subject
Has Abstractpub_date
2006-01-01 00:00:00pages
128-35issue
1eissn
1286-4579issn
1769-714Xpii
S1286-4579(05)00234-0journal_volume
8pub_type
杂志文章abstract::To obtain monkey-tropic viruses that are more closely related to HIV-1 than the original NL-DT5/NL-DT5R clones, we constructed six vif-chimeric and two site-specific vif-mutant viruses, and examined their growth ability. Different from NL-DT5/NL-DT5R, these viruses did not grow in monkey cells. We monitored the capabi...
journal_title:Microbes and infection
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journal_title:Microbes and infection
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2005.10.007
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journal_title:Microbes and infection
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journal_title:Microbes and infection
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doi:10.1016/j.micinf.2007.12.021
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2012.09.006
更新日期:2012-11-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2011.07.008
更新日期:2011-12-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2003.08.007
更新日期:2003-11-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.05.009
更新日期:2006-08-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(02)01625-8
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.04.023
更新日期:2006-08-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2012.07.018
更新日期:2012-11-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2007.02.017
更新日期:2007-05-01 00:00:00
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journal_title:Microbes and infection
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journal_title:Microbes and infection
pub_type: 杂志文章
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journal_title:Microbes and infection
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journal_title:Microbes and infection
pub_type: 杂志文章
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2009.03.001
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abstract::We previously reported prolonged HIV-1 transcriptional gene silencing by an RNA duplex targeting a sequence located within the NF-kappaB binding motif of the HIV-1 promoter in a susceptible HeLa cell line. Here we report extremely prolonged suppression of productive HIV-1 infection in a T-cell line (Molt-4) by a retro...
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journal_title:Microbes and infection
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
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abstract::Infection with Trypanosoma cruzi, the etiologic agent in Chagas disease, may result in heart disease. Over the last decades, Chagas disease endemic areas in Latin America have seen a dietary transition from the traditional regional diet to a Western style, fat rich diet. Previously, we demonstrated that during acute i...
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doi:10.1016/j.micinf.2018.07.001
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