Abstract：
:In this report, we examined the genetic diversity of HIV-1 strains circulating in the city of Beira, the second largest metropolitan area in Mozambique. A total of 131 blood samples, collected between August and October 2003 from antiretroviral-naïve individuals, were characterized with a combined approach consisting of heteroduplex mobility assay (HMA) subtyping for gag (n=74) and/or env (n=117) genes, and DNA sequence analysis of proviral env (C2V3C3, n=52), LTR (n=30) and/or pol (n=43) genomic regions. Aside from the identification, by bootscanning analysis, of a viral strain with a C/A1 mosaic C2V3C3 structure, classified as subtype A by env HMA, phylogenetic inference studies of the sequence data demonstrated the circulation of genetically diverse subtype C viruses, predominantly of the R5 type. Inspection of the LTR sequences revealed a pattern of structural and regulatory elements typical of subtype C, with 63.3% of the viruses showing three NF-kappaB binding sites. Analysis of the predicted protease sequences enabled us to detect a single primary mutation (I84V, n=1) associated with resistance to protease inhibitors (PI), while secondary mutations were highly prevalent, some of them in combinations which may confer PI resistance. Although an unexpectedly high rate (11.6%) of reverse transcriptase key mutations (V75A, K103N, Y181C, M184I, or P236L) was detected in the sequences analyzed, our data suggest the non-epidemic circulation of resistant viruses, and the absence of multi-class drug resistant viral strains.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Parreira R,Piedade J,Domingues A,Lobão D,Santos M,Venenno T,Baptista JL,Mussa SA,Barreto AT,Baptista AJ,Esteves Adoi
10.1016/j.micinf.2006.05.005subject
Has Abstractpub_date
2006-08-01 00:00:00pages
2442-51issue
9-10eissn
1286-4579issn
1769-714Xpii
S1286-4579(06)00221-8journal_volume
8pub_type
杂志文章abstract：:The objective of this study was to determine if inflammatory tolerance and enhancement of innate immune function could be induced by the Gram-positive cell wall component peptidoglycan (PGN). Male mice (C57BL6/J or C3H/HeJ, 8-12 weeks of age) were given intraperitoneal injections of 1mg PGN on 2 consecutive days. The ...
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2008.05.007
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2009-02-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2013.12.006
更新日期：2014-04-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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pub_type： 杂志文章
doi：10.1016/j.micinf.2006.04.025
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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