Abstract：
:Hippocampal soluble fraction stimulates acetylcholine (AcCho) synthesis of medial septal nuclei in explant culture system. This stimulating activity was purified from 10-12-day-old rat hippocampus. During purification, the activity was separated into two fractions and a previously unreported peptide was purified from one fraction. The structure of this novel peptide is acetyl-Ala-Ala-Asp-Ile-Ser-Gln-Trp-Ala-Gly-Pro-Leu and we designated it as hippocampal cholinergic neurostimulating peptide (HCNP). Synthesized HCNP and de-acetylated HCNP (free-HCNP) stimulated AcCho synthesis of medial septal nuclei culture, in a dose-dependent manner, but not cultures of corpus striatum or anterior spinal cord. Mean half-maximal concentrations of HCNP and free-HCNP in AcCho synthesis of medial septal nuclei culture were 1.0 +/- 0.3 x 10(-10) M and 1.0 +/- 0.6 x 10(-11) M, respectively. Affinity purified polyclonal antibody to the free-HCNP neutralized the activity of crude hippocampal extract, as well as synthetic HCNP and free-HCNP. These observations suggested that HCNP was present in the hippocampal extract and was involved in development of specific cholinergic neuron in central nervous system.
journal_name
Brain Resjournal_title
Brain researchauthors
Ojika K,Kojima S,Ueki Y,Fukushima N,Hayashi K,Yamamoto Mdoi
10.1016/0006-8993(92)90465-lsubject
Has Abstractpub_date
1992-02-14 00:00:00pages
164-71issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(92)90465-Ljournal_volume
572pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The effect of a single subcutaneous (s.c.) injection of the ultrapotent capsaicin analogue resiniferatoxin (RTX) on responses of adult rats to noxious thermal and mechanical stimulation was examined. The effects of RTX treatment on the nociceptive flexor reflex and activity-dependent increase in spinal excitability af...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)01444-8
更新日期：1997-03-28 00:00:00
abstract：:Photopic and scotopic spectral sensitivity of rhesus monkeys was determined before and after complete removal of the striate cortex. The monkeys were required to choose between a white and a series of monochromatic stimuli distributed throughout the visible spectrum. A modified method of limits was used to determine t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90346-7
更新日期：1975-08-08 00:00:00
abstract：:The effects of divalent and trivalent cations on the gamma-aminobutyric acid (GABA)-induced chloride current were studied with rat dorsal root ganglion neurons in primary culture using the whole-cell configuration of the patch-clamp technique. Lanthanum (La3+) reversibly potentiated the GABA-induced current with a hal...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91510-y
更新日期：1993-04-02 00:00:00
abstract：:High (2.5-5 micrograms) doses of ventral tegmental morphine, which normally facilitate brain stimulation reward, were found to cause a complete cessation of bar pressing for brainstem stimulation in animals pretreated with systemic pimozide (0.175-0.35 mg/kg). It was hypothesized that the behavioral failure was due to...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91402-9
更新日期：1989-01-16 00:00:00
abstract：:Mammalian neostriatum is composed of two neurochemically and neuroanatomically defined compartments, called the patches and matrix. The present study concerns a search for neurochemical molecules involved in formation of the striatal compartments. Using the monoclonal antibody CR-50, we here disclose a transient expre...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)02136-8
更新日期：1999-12-11 00:00:00
abstract：:This paper describes an electrochemical method for the detection of dopamine and serotonin in vitro. Dopamine and serotonin can be distinguished from each other without interference from metabolites. 3-4-dihydroxyphenylacetic acid and 5-hydroxyindoleacetic acid. The method also provides selective differentiation of do...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91224-9
更新日期：1989-08-21 00:00:00
abstract：:We studied the effects of transcranial magnetic stimulation (TMS) on muscle activation patterns and joint kinematics during oscillatory movements of a joint within a two-joint (elbow-wrist) synergy. Healthy young adults (N=8) performed cyclic movements in one of the joints while the other joint was or was not involved...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03958-6
更新日期：2003-01-31 00:00:00
abstract：:Neuronal expression of c-fos product in the cat rostral ventrolateral medulla was studied by immunohistochemistry. Spinally projecting neurons of the subretrofacial (SRF) nucleus were pre-labeled by retrograde transport of fluorescent latex microspheres from the lumbar cord. In 3 animals which were bled by approximate...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90099-u
更新日期：1992-03-20 00:00:00
abstract：:In this study, we have identified and characterized 5-HT3-like receptors in the rat medial prefrontal cortex (mPFc), an area with a moderate density of 5-HT3 binding sites, using the techniques of single unit recording and microiontophoresis. The microiontophoresis of the 5-HT3 receptor agonist 2-methylserotonin (2-Me...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91316-s
更新日期：1991-06-07 00:00:00
abstract：:The dopamine-releasing and depleting substance amphetamine (AMPH) can make cortical neurons susceptible to damage, and the prevention of hyperthermia, seizures and stroke is thought to block these effects. Here we report a 2-day AMPH treatment paradigm which affected only interneurons in three cortical regions with av...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03439-x
更新日期：2002-12-20 00:00:00
abstract：:The possibility that central adenosine A1 and A2a receptors mediate opiate dependence was examined in morphine-treated mice using radioligand binding methods. Mice treated with morphine for 72 h demonstrated significant increases in naloxone precipitated abstinence behaviors of jumping, wet-dog shakes, teeth chatterin...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90990-3
更新日期：1994-09-19 00:00:00
abstract：:Fasting induced a reduction in neuronal nitric oxide synthase (nNOS) mRNA in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of rats. The effect of intracerebroventricular (i.c.v.) administration of leptin on the nNOS mRNA level in the PVN and SON of fasting rats was studied by in situ hybridization his...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)02065-x
更新日期：1999-11-06 00:00:00
abstract：:Previous studies have reported that high concentrations of homocysteine and lower concentrations of vitamins B6, B12, and folate increase the risk for cognitive decline and pathology in aging populations. In this cross-sectional study, high resolution magnetic resonance imaging (MRI) scans and a 3-day food diary were ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.01.030
更新日期：2008-03-14 00:00:00
abstract：:The response properties of neurons in the region of striate cortex subserving central retina (0 degrees-2 degrees) and in a region of representation of parafoveal retina (4 degrees-7 degrees) were studied in unanesthetized paralyzed macaque monkeys. Neurons sensitive to the orientation of the stimulus in the visual fi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90240-1
更新日期：1975-12-12 00:00:00
abstract：:We employed a highly sensitive combination method of retrograde tracing and immunohistochemistry to identify calcitonin gene-related peptide (CGRP)-containing fiber pathways in the rat from the ventrolateral part of the ventrolateral thalamic nucleus (vl-vl) and the caudal continuation to the insular cortex. Biotin-wh...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)91211-9
更新日期：1985-09-30 00:00:00
abstract：:Various cytoskeletal proteins have been implicated in the formation of neurofibrillary tangles in Alzheimer's disease. A monoclonal antibody to non-phosphorylated neurofilament protein labels a distinct subset of pyramidal cells in the normal human cortex which have a distribution very similar to that of neurofibrilla...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90914-0
更新日期：1987-07-28 00:00:00
abstract：:Extracellular microelectrode recordings were made from a total of 868 neurons in the medullas of cats in regions known to contain high densities of respiratory-related neurons (solitary tract complex, nucleus ambiguus/retroambigualis, lateral tegmental field). Both the discharge patterns and the locations of units wer...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90979-x
更新日期：1984-03-19 00:00:00
abstract：:We have recently demonstrated that the formation of myelin sheaths in the optic nerve of young postnatal mice deficient in the myelin-associated glycoprotein (MAG) is retarded when compared to age-matched wild-type mice. In the present study, we analyzed whether impaired myelination of retinal ganglion cell axons is d...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00484-8
更新日期：1997-07-11 00:00:00
abstract：:The distribution and density of noradrenergic (NA) and serotonergic (5-HT) varicosities in the superior olive (SO) and periolivary region (PO) and their relationship to olivocochlear neurons was studied. Antibodies against 5-HT and the NA precursor enzyme dopamine beta-hydroxylase were utilized to examine the density ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01541-3
更新日期：1999-07-31 00:00:00
abstract：:The neuroactive steroid 5 beta-pregnan-3 alpha-ol-20-one (5 beta 3 alpha) modulates GABAA receptor function by potentiating postsynaptic GABA currents. While much is now known about the postsynaptic action of neurosteroids, far less is known about how they affect neurotransmission. We have investigated the synaptic ac...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00809-3
更新日期：1997-10-03 00:00:00
abstract：:The horizontal and vertical vestibulo ocular reflex (VOR) of head tilted (het) mutant mice was compared to C57BL/6 controls. Eye movements were recorded in darkness using a temporarily attached search coil. Contributions of semicircular canal versus otolith organ signals were investigated by providing a canal only (ve...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02505-8
更新日期：2003-05-16 00:00:00
abstract：:Both enkephalin and 5-hydroxytryptamine (5-HT) have been implicated in neural mechanisms underlying the central control of respiration. In view of the role of the nuclei tractus solitarii (NTS) in respiratory regulation, we carried out a study in artificially ventilated, chloralose-anaesthetized cats of the effects of...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)91515-x
更新日期：1985-02-18 00:00:00
abstract：:Mitochondrial electron transport chain (ETC) function is selectively reduced in multiple tissues, including brain, from patients with Parkinson's disease (PD) and Alzheimer's disease (AD). The ETC defects are specific to each illness, involve complex I in PD and complex IV in AD, are transferable with mitochondrial DN...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00429-0
更新日期：1997-08-15 00:00:00
abstract：:Factors underlying paired-pulse facilitation (PPF) were studied by intracellular and field recordings of CA1 neurons in the hippocampal slice in vitro, following stimulation of the Schaffer collaterals apical dendritic afferents. Similar magnitudes of PPF were found using the slopes or peaks of the excitatory postsyna...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90209-7
更新日期：1994-07-04 00:00:00
abstract：:Glucocorticoids bind with high affinity to intracellular receptors located in high density within discrete regions of the rodent and primate brain. The binding of [3H]corticosterone was compared in the brains of male vs female rats. The number and affinity of cytosol receptors in the hippocampus and hypothalamus were ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)91153-9
更新日期：1985-09-16 00:00:00
abstract：:Secondary hemorrhage after thrombolysis in ischemic stroke is an important complication, which has been difficult to study in preclinical disease models. We have established and characterized a model of thromboembolic middle cerebral artery occlusion in rats. Advantages of this model include a very low rate of spontan...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.10.067
更新日期：2011-01-12 00:00:00
abstract：:The peroxidase-antiperoxidase (PAP) immunohistochemical technique was used to identify neurons in the basolateral amygdaloid nucleus (BL) that contain vasoactive intestinal polypeptide (VIP), somatostatin (SOM) or cholecystokinin (CCK). Examination of immunostained neurons demonstrated that most, if not all, of these ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90266-5
更新日期：1985-07-08 00:00:00
abstract：:Altered expression of neurotrophins may contribute to the pathogenesis of schizophrenia. Several studies suggest sex steroid hormones may be involved in the regulation of brain-derived neurotrophic factor (BDNF) signaling, as well as the symptoms of schizophrenia. This study aimed to identify sex differences in BDNF-T...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.01.060
更新日期：2011-04-12 00:00:00
abstract：:Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) is a potent neuroexcitatory compound which acts at the quisqualate class of excitatory amino acid receptors. In this study we describe the pharmacological characteristics and anatomical distribution of [3H]AMPA binding sites in rat brain using quantitativ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90636-x
更新日期：1984-12-17 00:00:00
abstract：:We used in situ hybridization and immunocytochemistry to investigate the presence of GABA in neurons that express the type 3 serotonin receptor (5-HT3R). Quantitative analysis indicated that more than 90% of 5-HT3R expressing cells are GABAergic in the neocortex and hippocampus. The co-existence of 5-HT3R and GABA in ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00557-4
更新日期：1996-08-26 00:00:00
