当前位置： SCI文献检索 > VACCINE期刊下所有文献 > Reporter gene expression in dendritic cells after gene gun administration of plasmid DNA.

Reporter gene expression in dendritic cells after gene gun administration of plasmid DNA.

Abstract：

:Dendritic cells (DC) play an integral role in plasmid DNA vaccination. However, the interaction between plasmid DNA and DC in vivo is incompletely understood. In this report, we utilise the sheep pseudoafferent cannulation model to examine the interaction between plasmid DNA encoding enhanced green fluorescent protein (pEGFP) and afferent lymph DC (ALDC) following gene gun administration. The results show that peaks of fluorescent ALDC tended to appear around days 1-4 and 9-13, then erratically thereafter for up to 2 months. Phenotypic analysis showed that EGFP+ ALDC expressed MHC class II, WC6, CD1b, and SIRPalpha markers. Plasmid, detected by PCR, was found in lymph cells and cell-free plasma on a daily basis, and was present variably for up to 2 months. Plasmid was also detected in purified CD1b+ ALDC, but the presence of plasmid did not correlate with EGFP expression by ALDC. Free EGFP in afferent lymph plasma was detectable by luminometry only after three administrations of the plasmid. The results show that gene gun administered pEGFP persisted for extended periods after a single administration, leeching out of skin on a daily basis. The plasmid was associated with both the cellular and fluid components of afferent lymph. EGFP protein appeared in afferent lymph in a pulsatile manner, but associated only with ALDC.

journal_name

Vaccine

journal_title

Vaccine

authors

Watkins C,Hopkins J,Harkiss G

doi

10.1016/j.vaccine.2005.03.023

subject

Has Abstract

pub_date

2005-07-21 00:00:00

pages

4247-56

issue

33

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(05)00402-0

journal_volume

23

pub_type

杂志文章

相关文献

VACCINE文献大全
  • Coxiella burnetii: host and bacterial responses to infection.

    abstract：:Designation as a Category B biothreat agent has propelled Coxiella burnetii from a relatively obscure, underappreciated, "niche" microorganism on the periphery of bacteriology, to one of possibly great consequence if actually used in acts of bioterrorism. Advances in the study of this microorganism proceeded slowly, p...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2007.08.002

    authors： Waag DM

    更新日期：2007-10-16 00:00:00

  • Protection provided by a recombinant ALVAC(®)-WNV vaccine expressing the prM/E genes of a lineage 1 strain of WNV against a virulent challenge with a lineage 2 strain.

    abstract：:The emergence of lineage 2 strains of WNV in Europe as a cause of clinical disease and mortality in horses raised the question whether the existing WNV vaccines, all based on lineage 1 strains, protect against circulating lineage 2 strains of WNV. In the present paper we have determined the level of cross protection p...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.04.058

    authors： Minke JM,Siger L,Cupillard L,Powers B,Bakonyi T,Boyum S,Nowotny N,Bowen R

    更新日期：2011-06-20 00:00:00

  • Alterations in serotype-specific B cell responses to the 13-valent pneumococcal conjugate vaccine in aging HIV-infected adults.

    abstract：BACKGROUND:Advanced age and human immunodeficiency virus (HIV) infection are associated with increased pneumococcal disease risk. The impact of these factors on cellular responses to vaccination is unknown. METHODS:HIV-infected (HIV+) individuals 50-65 years old with CD4(+) Tcells/μl (CD4) >200 on antiretroviral thera...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.12.013

    authors： Ohtola JA,Khaskhely NM,Saul-Mcbeth JL,Iyer AS,Leggat DJ,Khuder SA,Westerink MAJ

    更新日期：2016-01-20 00:00:00

  • Development and evaluation of live attenuated Salmonella vaccines in newly hatched duckings.

    abstract：:Domestic ducks remain a major source of zoonotic Salmonella enterica infections for man worldwide and approaches to protection should include vaccine-mediated immunity. With this in mind we developed several genetically defined mutants in a virulent duck Salmonella typhimurium isolate TT-1. From initial tests for viru...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.09.004

    authors： Tang T,Gao Q,Barrow P,Wang M,Cheng A,Jia R,Zhu D,Chen S,Liu M,Sun K,Yang Q,Chen X

    更新日期：2015-10-13 00:00:00

  • Independent but not synergistic enhancement to the immunogenicity of DNA vaccine expressing HIV-1 gp120 glycoprotein by codon optimization and C3d fusion in a mouse model.

    abstract：:The ability to elicit humoral and cell-mediated immune (CMI) responses from DNA immunization by combinational use of codon optimization and C3d component of complement was evaluated in this study. DNA vaccines that express either the wild type or the codon optimized gp120 gene coding for the envelope (Env) glycoprotei...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2003.09.054

    authors： Liu F,Mboudjeka I,Shen S,Chou TH,Wang S,Ross TM,Lu S

    更新日期：2004-04-16 00:00:00

  • Safety of AS03-adjuvanted influenza vaccines: A review of the evidence.

    abstract：:Clinical and post-licensure data have demonstrated that AS03-adjuvanted inactivated split virion vaccines, many with reduced antigen content, are effective against influenza infection. The objective of this review is to provide a comprehensive assessment of the safety of trivalent seasonal, monovalent pre-pandemic and...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2019.04.048

    authors： Cohet C,van der Most R,Bauchau V,Bekkat-Berkani R,Doherty TM,Schuind A,Tavares Da Silva F,Rappuoli R,Garçon N,Innis BL

    更新日期：2019-05-21 00:00:00

  • Midwives' knowledge, attitudes and confidence in discussing maternal and childhood immunisation with parents: A national study.

    abstract：INTRODUCTION:Despite the enormous benefits of childhood and maternal immunisation to individual and population health, the uptake of maternal vaccines during pregnancy remains suboptimal. Midwives are a trusted information source for parents and play an important role in the provision of immunisation information. Under...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2019.10.006

    authors： Frawley JE,McKenzie K,Sinclair L,Cummins A,Wardle J,Hall H

    更新日期：2020-01-10 00:00:00

  • Secretory immune responses in the mouse vagina after parenteral or intravaginal immunization with an immunostimulating complex (ISCOM).

    abstract：:Immunostimulating complexes (ISCOMs) are subunit vaccines that are particularly effective in producing immunity against systemic viral infections, but their effectiveness against mucosal infections has received little attention. To study their ability to produce mucosal immune responses in the female reproductive trac...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(91)90269-c

    authors： Thapar MA,Parr EL,Bozzola JJ,Parr MB

    更新日期：1991-02-01 00:00:00

  • Neospora caninum antigens displaying virus-like particles as a bivalent vaccine candidate against neosporosis.

    abstract：:Neospora caninum is a causative and transmissible agent of dog and bovine neosporosis. The resulting reproductive failures in infected cattle lead to significant economic losses worldwide. However, there is no satisfactory treatment or vaccine currently available to combat this pathogen. Thus, the development of appro...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2019.09.002

    authors： Xu J,Hiramatsu R,Suhaimi H,Kato T,Fujimoto A,Tokiwa T,Ike K,Park EY

    更新日期：2019-10-08 00:00:00

  • Protective immunity against herpes simplex virus generated by DNA vaccination compared to natural infection.

    abstract：:To evaluate the utility of plasmid DNA vaccination against disease caused by herpes simplex virus (HSV), we compared the strength of protection against lethal challenge following natural virus infection with that following vaccination with a plasmid encoding HSV glycoprotein gD (gD-DNA). We further determined the cell...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(00)00380-7

    authors： Nass PH,Elkins KL,Weir JP

    更新日期：2001-01-08 00:00:00

  • Human rotavirus genotypes in hospitalized children, South Korea, April 2005 to March 2007.

    abstract：:Availability of new rotavirus vaccines highlights the need to maintain and enhance rotavirus strain surveillance. We collected stool samples from children with gastroenteritis admitted to eight hospitals in South Korea from April 2005 to March 2007. Of the 6057 samples collected, 1337 (22%) were positive for rotavirus...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.09.002

    authors： Lee SY,Hong SK,Lee SG,Suh CI,Park SW,Lee JH,Kim JH,Kim DS,Kim HM,Jang YT,Ma SH,Kim SY,Sohn YS,Kang JH,Paik SY

    更新日期：2009-11-20 00:00:00

  • Clinical experience with the meningococcal B vaccine, Bexsero(®): Prospects for reducing the burden of meningococcal serogroup B disease.

    abstract：:Although rare, invasive meningococcal disease remains an important cause of mortality and morbidity in children and young adults. Vaccines have been successfully introduced to help protect against meningococcal disease caused by serogroups A, C, W and Y, but until recently, a vaccine for serogroup B (MenB) was not ava...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2015.11.057

    authors： Watson PS,Turner DP

    更新日期：2016-02-10 00:00:00

  • Oral immunization with a Streptococcal pneumoniae polysaccharide conjugate vaccine in enterocoated microparticles induces serum antibodies against type specific polysaccharides.

    abstract：:The authors examined the antibody responses of mice orally immunized with pneumococcal polysaccharide (type 23F) or a pneumococcal polysaccharide conjugated to the outer membrane protein complex of Neisseria meningitides (23F-OMPC). These antigens were administered either in solution or entrapped within microcapsules....

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(98)00118-2

    authors： Flanagan MP,Michael JG

    更新日期：1999-01-01 00:00:00

  • Immunization with a cocktail of antigens fused with OprI reduces Neospora caninum vertical transmission and postnatal mortality in mice.

    abstract：:OprI is an outer membrane lipoprotein from Pseudomonas aeruginosa, and when fused to a recombinant antigen, will exert adjuvant properties by engaging Toll-like receptor 2, leading to dendritic cell activation. Previous studies have shown that the Neospora caninum (Nc) antigens NcPDI, NcROP2 and NcROP40 are implicated...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.11.060

    authors： Aguado-Martínez A,Basto AP,Tanaka S,Ryser LT,Nunes TP,Ortega-Mora LM,Arranz-Solís D,Leitão A,Hemphill A

    更新日期：2019-01-14 00:00:00

  • Porcine interleukin-3 enhances DNA vaccination against classical swine fever.

    abstract：:DNA vectors can be used to deliver vaccine antigens that stimulate effective protective immunity in mice, but in larger, outbred animal species, the protective efficacy is lower or large doses of DNA are required. These data demonstrate that porcine interleukin-3 (IL-3) when delivered to pigs by DNA vector or in low d...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2006.01.023

    authors： Andrew M,Morris K,Coupar B,Sproat K,Oke P,Bruce M,Broadway M,Morrissy C,Strom D

    更新日期：2006-04-12 00:00:00

  • Safety and immunogenicity of heterologous prime-boost immunization with viral-vectored malaria vaccines adjuvanted with Matrix-M™.

    abstract：:The use of viral vectors in heterologous prime-boost regimens to induce potent T cell responses in addition to humoral immunity is a promising vaccination strategy in the fight against malaria. We conducted an open-label, first-in-human, controlled Phase I study evaluating the safety and immunogenicity of Matrix-M adj...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.09.028

    authors： Venkatraman N,Anagnostou N,Bliss C,Bowyer G,Wright D,Lövgren-Bengtsson K,Roberts R,Poulton I,Lawrie A,Ewer K,V S Hill A

    更新日期：2017-10-27 00:00:00

  • Influenza vaccination rates in Ontario children: implications for universal childhood vaccination policy.

    abstract：:The aims of this study were to estimate influenza vaccination coverage for children during the 2006-2007 influenza season in Ontario, Canada, where universal vaccination is available, and to compare the rate among children aged 6-23 months with corresponding rates from other Canadian provinces that specifically target...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.02.017

    authors： Moran K,Maaten S,Guttmann A,Northrup D,Kwong JC

    更新日期：2009-04-14 00:00:00

  • Carrier-dependent specificity of antibodies to a conserved peptide determinant of gp120.

    abstract：:Amino acid residues 421-436 constitute a comparatively conserved determinant of gp120 that participates in the binding of host cell CD4 receptors by HIV-1. We compared the immunogenicity of synthetic Cys-gp120 (421-436) conjugated to KLH via the N terminal Cys residue (KLH-I) and gp120 (421-436) extended at its N term...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(02)00504-2

    authors： Karle S,Nishiyama Y,Taguchi H,Zhou YX,Luo J,Planque S,Hanson C,Paul S

    更新日期：2003-03-07 00:00:00

  • Immunogenicity of porcine P[6], P[7]-specific △VP8* rotavirus subunit vaccines with a tetanus toxoid universal T cell epitope.

    abstract：:Currently, commercial porcine rotavirus vaccines remain varied limitations. The objective of this study is to develop an alternative porcine rotavirus subunit vaccine candidate by parenteral administration, which enables to elicit robust immune responses against most prevalence porcine rotavirus strains. The bacterial...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.07.011

    authors： Wen X,Wei X,Ran X,Ni H,Cao S,Zhang Y

    更新日期：2015-08-26 00:00:00

  • Influenza vaccination coverage in adults belonging to defined target groups, Germany, 2003/2004.

    abstract：:In 2003, a nationwide cross-sectional study was conducted to assess the current influenza vaccination coverage of the adult population of Germany, especially in persons belonging to defined target groups. Of 666 surveyed persons, 190 (adjusted 24%) had received influenza vaccination and 419 participants belonged to at...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.12.020

    authors： Wiese-Posselt M,Leitmeyer K,Hamouda O,Bocter N,Zöllner I,Haas W,Ammon A

    更新日期：2006-03-24 00:00:00

  • Enhanced mucosal and systemic immune response with intranasal immunization of mice with HIV peptides entrapped in PLG microparticles in combination with Ulex Europaeus-I lectin as M cell target.

    abstract：:The predominant route of HIV infection is through the sexual transmission via M cells. Most of the peptide and protein vaccines show poor transport across the epithelial barrier and are commonly administered by parenteral route. In the present study four HIV peptides from envelope (gp 41-LZ (leucine zipper), gp 41-FD ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.06.031

    authors： Manocha M,Pal PC,Chitralekha KT,Thomas BE,Tripathi V,Gupta SD,Paranjape R,Kulkarni S,Rao DN

    更新日期：2005-12-01 00:00:00

  • Immunization with DnaJ (hsp40) could elicit protection against nasopharyngeal colonization and invasive infection caused by different strains of Streptococcus pneumoniae.

    abstract：:Increasing mortality, morbidity and economic costs have been paid to pneumococcal diseases every year. Currently, vaccination is the most promising strategy to reduce the occurrence of pneumococcal infection. In this study, we investigated the protective efficacy of immunization with recombinant DnaJ (hsp40) protein a...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2010.12.126

    authors： Cui Y,Zhang X,Gong Y,Niu S,Yin N,Yao R,Xu W,Li D,Wang H,He Y,Cao J,Yin Y

    更新日期：2011-02-17 00:00:00

  • Bordetella pertussis adenylate cyclase delivers chemically coupled CD8+ T-cell epitopes to dendritic cells and elicits CTL in vivo.

    abstract：:The adenylate cyclase (CyaA) produced by Bordetella pertussis is able to deliver CD8+ and CD4+ T-cell epitopes genetically grafted within the catalytic domain of the molecule into antigen presenting cells in vivo. We develop now a new approach in which peptides containing CD8+ epitopes are chemically linked to CyaA. W...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2004.07.018

    authors： Fayolle C,Bauche C,Ladant D,Leclerc C

    更新日期：2004-12-16 00:00:00

  • Interferon-gamma-inducing oral vaccination with Leishmania amazonensis antigens protects BALB/c and C57BL/6 mice against cutaneous leishmaniasis.

    abstract：:The induction of oral tolerance against disease-inducing antigens has emerged as a feasible strategy to prevent immunopathologies. In this study, we investigated the effect of oral immunization with whole antigens of Leishmania amazonensis promastigotes (LaAg) on murine cutaneous leishmaniasis. We found that two oral ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(03)00427-4

    authors： Pinto EF,de Mello Cortezia M,Rossi-Bergmann B

    更新日期：2003-09-08 00:00:00

  • Status of natural infection with Japanese encephalitis virus in Japan: prevalence of antibodies to the nonstructural 1 protein among humans and horses.

    abstract：:The literature on natural infections with Japanese encephalitis virus in Japan and subclinical:clinical infection rates was summarized. To detect natural infections, conventional serologic methods were used in the past, while nonstructural 1 protein-based methods have been used recently. Annual infection rates in huma...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.09.052

    authors： Konishi E

    更新日期：2009-11-23 00:00:00

  • Synthetic luteinizing hormone releasing hormone (LHRH) vaccine for effective androgen deprivation and its application to prostate cancer immunotherapy.

    abstract：:We have designed a peptide-based immunotherapeutic vaccine for treatment of androgen-responsive prostate cancer. The vaccine targets the luteinizing hormone-releasing hormone (LHRH) decapeptide that results in an androgen-deprivation immunotherapy. The design elements of the peptide immunogens are the LHRH peptide or ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2003.08.044

    authors： Finstad CL,Wang CY,Kowalski J,Zhang M,Li ML,Li XM,Xia WG,Bosland MC,Murthy KK,Walfield AM,Koff WC,Zamb TJ

    更新日期：2004-03-12 00:00:00

  • Revisiting assumptions about age-based mixing representations in mathematical models of sexually transmitted infections.

    abstract：BACKGROUND:Sexual mixing between heterogeneous population subgroups is an integral component of mathematical models of sexually transmitted infections (STIs). This study compares the fit of different mixing representations to survey data and the impact of different mixing assumptions on the predicted benefits of hypoth...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.07.058

    authors： Easterly CW,Alarid-Escudero F,Enns EA,Kulasingam S

    更新日期：2018-09-05 00:00:00

  • Immunogenicity of candidate hepatitis E virus DNA vaccine expressing complete and truncated ORF2 in mice.

    abstract：:Hepatitis E virus (HEV) is a major cause of enterically transmitted acute hepatitis of adults in developing nations. Our present studies show that, the complete ORF2 gene (1-660 amino acids, a.a.) coding for capsid protein of HEV as candidate DNA vaccine induced significant specific humoral and cellular immune respons...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.03.040

    authors： Deshmukh TM,Lole KS,Tripathy AS,Arankalle VA

    更新日期：2007-05-30 00:00:00

  • Factors influencing influenza vaccination uptake in an elderly, community-based sample.

    abstract：:This study investigated predictors of influenza vaccination uptake since the introduction of the policy to target over 65s. Four hundred and forty-four participants completed a cross-sectional structured interview. The predictors of vaccination uptake were: having a doctor or nurse who explained why the vaccination is...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2004.12.031

    authors： Burns VE,Ring C,Carroll D

    更新日期：2005-05-20 00:00:00

  • Long-term immunogenicity after single and booster dose of a live attenuated hepatitis A vaccine: results from 8-year follow-up.

    abstract：:Live, attenuated hepatitis A vaccines are used widely in China but there is uncertainty regarding the persistence of vaccine-induced anti-HAV antibodies after single dose and booster dose administrated at month 12. A large scale clinical trial to evaluate the live, attenuated hepatitis A vaccine was conducted in Hebei...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2006.08.004

    authors： Wang XY,Xu ZY,Ma JC,von Seidlein L,Zhang Y,Hao ZY,Han OP,Zhang YL,Tian MY,Ouyang PY,Zhang ZY,Han CQ,Xing ZC,Chen JC

    更新日期：2007-01-05 00:00:00