Immunization with a cocktail of antigens fused with OprI reduces Neospora caninum vertical transmission and postnatal mortality in mice.


:OprI is an outer membrane lipoprotein from Pseudomonas aeruginosa, and when fused to a recombinant antigen, will exert adjuvant properties by engaging Toll-like receptor 2, leading to dendritic cell activation. Previous studies have shown that the Neospora caninum (Nc) antigens NcPDI, NcROP2 and NcROP40 are implicated in host cell interactions and are promising vaccine candidates. In two independent experiments, the efficacy of a polyvalent vaccine formulation composed of OprI-NcPDI, OprI-NcROP2 and OprI-NcROP40 (collectively named O-Ags) was assessed in non-pregnant and pregnant Balb/c mouse models challenged with tachyzoites of the high-virulence isolate Nc-Spain7. Parameters that were investigated were clinical signs, fertility, parasite burden in adult mice, humoral and cellular immune responses at different time-points prior to and after challenge infection, vertical transmission and post-natal survival of offspring mice, all to explore potential correlations with efficacy. Vaccination of mice with O-Ags induced a mixed Th1/Th2 immune response in adult mice and led to significantly increased protection against cerebral infection. Vaccination with O-Ags also resulted in reduced vertical transmission, and postnatal disease in offspring was significantly inhibited at a rate not observed in mice infected with a high-virulence isolate to date. However, O-Ags mixed with TLR ligands targeting TLR3 and TLR7, which are known to induce clear Th1-biased responses, or vaccination with OprI fused to the non-N. caninum antigen ovalbumin (OprI-OVA) did not confer protection.






Aguado-Martínez A,Basto AP,Tanaka S,Ryser LT,Nunes TP,Ortega-Mora LM,Arranz-Solís D,Leitão A,Hemphill A




Has Abstract


2019-01-14 00:00:00
















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    abstract::In a phase I clinical trial, one hundred healthy young adults were randomized to receive two doses 28 days apart of an inactivated, subvirion vaccine containing 15 or 45microg of influenza A/H5N1 hemagglutinin (HA) by the intramuscular (IM) route, or 3 or 9microg of H5 HA by the intradermal(ID) route. Seventy-seven su...


    pub_type: 杂志文章,随机对照试验


    authors: Patel SM,Atmar RL,El Sahly HM,Cate TR,Keitel WA

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  • Evaluating functional antibodies in rhesus monkeys immunized with hepatitis B virus surface antigen vaccine with novel adjuvant formulations.

    abstract::Effective and safe novel adjuvants are of great interest to the vaccine research community. In this study, we describe our evaluation of adjuvant formulations containing a TLR9 agonist adjuvant (ISS1018) or ISCOMATRIX™ adjuvant for a two-dose regimen of hepatitis B virus surface antigen virus-like particle vaccine in ...


    pub_type: 杂志文章


    authors: Freed DC,Towne VM,Casimiro DR,Zhao Q,Fu TM

    更新日期:2011-11-21 00:00:00

  • Correlation of specific antibody titre and avidity with protection in cattle immunized against Fasciola hepatica.

    abstract::Cattle produce specific serum antibody mainly of the IgG1 isotype in response to infection with the liver fluke, Fasciola hepatica. In these animals a positive correlation between fluke-specific serum IgG1 levels and fluke-burden in non-immunized infected animals was observed. In contrast, immunization of cattle with ...


    pub_type: 杂志文章


    authors: Mulcahy G,O'Connor F,McGonigle S,Dowd A,Clery DG,Andrews SJ,Dalton JP

    更新日期:1998-05-01 00:00:00

  • Enteral immunization and challenge of volunteers given enterotoxigenic E. coli CFA/II encapsulated in biodegradable microspheres.

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    pub_type: 临床试验,杂志文章


    authors: Tacket CO,Reid RH,Boedeker EC,Losonsky G,Nataro JP,Bhagat H,Edelman R

    更新日期:1994-11-01 00:00:00

  • Tetanus immunity among Italians born after the introduction of mandatory vaccination of children.

    abstract::In Italy systematic mandatory tetanus immunization of children started in 1968. In a national sample of 241 young males born after 1968 the prevalence of non-immune subjects was 11.2% (5.5% in the North-Centre, 15.9% in the South-Islands; p < 0.02). Comparing these data with the corresponding figure obtained in a prev...


    pub_type: 杂志文章


    authors: D'Amelio R,Stroffolini T,Wirz M,Biselli R,Matricardi PM,Gentili G,Collotti C,Pasquini P

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    abstract:BACKGROUND:Routine administration of all age-appropriate doses of vaccines during the same visit is recommended for children by the National Vaccine Advisory Committee (NVAC) and the Advisory Committee on Immunization Practices (ACIP). METHODS:Evaluate the potentially achievable vaccination coverage for ≥4 doses of di...


    pub_type: 杂志文章


    authors: Zhao Z,Smith PJ,Hill HA

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  • Identification of Setaria cervi heat shock protein 70 by mass spectrometry and its evaluation as diagnostic marker for lymphatic filariasis.

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    pub_type: 杂志文章


    authors: Srivastava S,Srikanth E,Liebau E,Rathaur S

    更新日期:2010-02-03 00:00:00

  • Reduced oligomeric and vascular amyloid-beta following immunization of TgCRND8 mice with an Alzheimer's DNA vaccine.

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    pub_type: 杂志文章


    authors: DaSilva KA,Brown ME,McLaurin J

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  • A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus.

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    pub_type: 杂志文章


    authors: Johnson MA,Pooley C,Ignjatovic J,Tyack SG

    更新日期:2003-06-20 00:00:00

  • Influenza A vaccines using linear expression cassettes delivered via electroporation afford full protection against challenge in a mouse model.

    abstract::Alternative DNA vaccine constructs such as fully synthetic linear expressing cassettes (LECs) offer the advantage of accelerated manufacturing techniques as well as the lack of both antibiotic resistance genes and bacterial contaminants. The speed of manufacture makes LEC technology a possible future vaccination strat...


    pub_type: 杂志文章


    authors: Shen X,Söderholm J,Lin F,Kobinger G,Bello A,Gregg DA,Broderick KE,Sardesai NY

    更新日期:2012-11-06 00:00:00

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    abstract::Polysaccharide-protein conjugate vaccines against Haemophilus influenzae type b (Hib) and Streptococcus pneumoniae have proven efficacy against radiologically confirmed pneumonia. Measurement of pneumonia incidence provides a platform to estimate of the vaccine-preventable burden. Over 24 months, we conducted surveill...


    pub_type: 杂志文章


    authors: Roca A,Sigaúque B,Quintó L,Morais L,Berenguera A,Corachan M,Ribó JL,Naniche D,Bassat Q,Sacoor Ch,Nhalungo D,Macete E,Schuchat A,Soriano-Gabarró M,Flannery B,Alonso PL

    更新日期:2010-07-05 00:00:00

  • Vaccination against the 2009 pandemic influenza A (H1N1) among healthcare workers in the major teaching hospital of Sicily (Italy).

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    pub_type: 杂志文章


    authors: Amodio E,Anastasi G,Marsala MG,Torregrossa MV,Romano N,Firenze A

    更新日期:2011-02-04 00:00:00

  • Effectiveness of mouse brain-derived inactivated Japanese encephalitis vaccine in Thai National Immunization Program: a case-control study.

    abstract::Mouse brain-derived, purified inactivated Japanese encephalitis vaccine (MB JEV) has been locally produced and introduced into Thai National Immunization Program (NIP) since 1990. However, MB JEV effectiveness has been concerned, since 30-40% of JE cases received JE vaccines were recently reported in two descriptive s...


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    authors: Muangchana C,Henprasertthae N,Nurach K,Theppang K,Yoocharoen P,Varinsathien P,Techathawat S,Sanohsieng S,Anantapreecha S

    更新日期:2012-01-05 00:00:00

  • Romurtide, a synthetic muramyl dipeptide derivative, accelerates peripheral platelet recovery in nonhuman primate chemotherapy model.

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    pub_type: 杂志文章


    authors: Namba K,Nitanai H,Otani T,Azuma I

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    authors: Singh R,Singh S,Briles DE,Taub DD,Hollingshead SK,Lillard JW Jr

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    authors: Neal LM,McCarthy EA,Morris CR,Mantis NJ

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    authors: Leboucher B,Sentilhes L,Abbou F,Henry E,Grimprel E,Descamps P

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    authors: Kachko A,Kochneva G,Sivolobova G,Grazhdantseva A,Lupan T,Zubkova I,Wells F,Merchlinsky M,Williams O,Watanabe H,Ivanova A,Shvalov A,Loktev V,Netesov S,Major ME

    更新日期:2011-12-09 00:00:00

  • Protection induced in mice against a lethal orthopox virus by the Lister strain of vaccinia virus and modified vaccinia virus Ankara (MVA).

    abstract::The Lister (Elstree) strain of vaccinia virus, used in Israel for vaccination against smallpox, was studied in tissue cultures and in a mouse model. The virus failed to reach the brain of the mice when inoculated intranasally at a dose of 500,000 plaque forming units, but was lethal for 50% of them, when injected intr...


    pub_type: 杂志文章


    authors: Abdalrhman I,Gurt I,Katz E

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  • Hepatitis A virus seroprevalence by age and world region, 1990 and 2005.

    abstract:OBJECTIVE:To estimate current age-specific rates of immunity to hepatitis A virus (HAV) in world regions by conducting a systematic review and meta-analysis of published data. The estimation of the global burden of hepatitis A and policies for public health control are dependent on an understanding of the changing epid...


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    authors: Oteri J,Idi Hussaini M,Bawa S,Ibizugbe S,Lambo K,Mogekwu F,Wiwa O,Seaman V,Kolbe-Booysen O,Braka F,Nsubuga P,Shuaib F

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    authors: Shimizu H

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    authors: Martinon-Torres F,Gimenez-Sanchez F,Bernaola-Iturbe E,Diez-Domingo J,Jiang Q,Perez JL

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    pub_type: 杂志文章


    authors: Hsieh HV,Stewart B,Hauer P,Haaland P,Campbell R

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    authors: Zhang-Barber L,Turner AK,Barrow PA

    更新日期:1999-06-04 00:00:00

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    authors: Mosley YY,Wu CC,Lin TL

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    pub_type: 杂志文章


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    更新日期:2001-06-14 00:00:00

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    pub_type: 杂志文章


    authors: Saito T,Wada H,Yamasaki M,Miyata H,Nishikawa H,Sato E,Kageyama S,Shiku H,Mori M,Doki Y

    更新日期:2014-10-14 00:00:00