Abstract：
:Dynamic properties of type IV pili are essential for their function in bacterial infection, twitching motility and gene transfer. Laser tweezers are versatile tools to study the molecular mechanism underlying pilus dynamics at the single molecule level. Recently, these optical tweezers have been used to monitor pilus elongation and retraction in vivo at a resolution of several nanometers. The force generated by type IV pili exceeds 100 pN making pili the strongest linear motors characterized to date. The study of pilus dynamics at the single molecule level sheds light on kinetics, force generation, switching and mechanics of the Neisseria gonorrhoeae pilus motor.
journal_name
Curr Opin Microbioljournal_title
Current opinion in microbiologyauthors
Maier Bdoi
10.1016/j.mib.2005.04.002subject
Has Abstractpub_date
2005-06-01 00:00:00pages
344-9issue
3eissn
1369-5274issn
1879-0364pii
S1369-5274(05)00040-8journal_volume
8pub_type
杂志文章,评审abstract：:It is well accepted that bacterial pathogens growing in a biofilm are recalcitrant to the action of most antibiotics and are resistant to the innate immune system. New treatment modalities are greatly warranted to effectively eradicate these infections. However, bacteria growing in a biofilm are metabolically unique i...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2010.09.007
更新日期：2010-10-01 00:00:00
abstract：:Single microbial cells can now be sequenced using DNA amplified by the Multiple Displacement Amplification (MDA) reaction. The few femtograms of DNA in a bacterium are amplified into micrograms of high molecular weight DNA suitable for DNA library construction and Sanger sequencing. The MDA-generated DNA also performs...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2007.08.005
更新日期：2007-10-01 00:00:00
abstract：:Some RNA virus genomes bear 5'-triphosphates, which can be recognized in the cytoplasm of infected cells by host proteins that mediate anti-viral immunity. Both the innate sensor RIG-I and the interferon-induced IFIT proteins bind to 5'-triphosphate viral RNAs. RIG-I signals for induction of interferons during RNA vir...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2013.04.009
更新日期：2013-08-01 00:00:00
abstract：:Just as we thought that we know everything about superantigens, new molecular and structural studies indicate that we have only just begun to unravel the secrets of these fascinating molecules. Recent structure-function analysis of superantigens from Gram-positive bacteria, with emphasis on their interaction with majo...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s1369-5274(98)80143-4
更新日期：1998-02-01 00:00:00
abstract：:Clustered, regularly interspaced, short, palindromic repeats (CRISPR) loci, together with their CRISPR-associated (Cas) proteins, provide bacteria and archaea with adaptive immunity against invasion by bacteriophages, plasmids, and other mobile genetic elements. These host defenses impart selective pressure on phages ...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2017.06.003
更新日期：2017-06-01 00:00:00
abstract：:RNA sequencing has emerged as the premier approach to study bacterial transcriptomes. While the earliest published studies analyzed the data qualitatively, the data are readily digitized and lend themselves to quantitative analysis. High-resolution RNA sequence (RNA-seq) data allows transcriptional features (promoters...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2014.11.011
更新日期：2015-02-01 00:00:00
abstract：:The DNA-binding domains of eucaryotic GATA factors comprise a four-cysteine Zn finger and an adjacent basic region. Fungal GATA factors regulate nitrogen metabolism, light induction, siderophore biosynthesis and mating-type switching. Hydrophobic interactions determine binding-site specificity. Interactions with other...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s1369-5274(00)00063-1
更新日期：2000-04-01 00:00:00
abstract：:Streptococcus agalactiae (group B Streptococcus, GBS), a normal constituent of the intestinal microbiota is the major cause of human neonatal infections and a worldwide spread 'hypervirulent' clone, GBS ST-17, is strongly associated with neonatal meningitis. Adhesion to epithelial and endothelial cells constitutes a k...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2011.12.002
更新日期：2012-02-01 00:00:00
abstract：:High-throughput identification of proteins and their accurate partial sequencing by shotgun nanoLC-MS/MS are now feasible for any cellular model at a full genomic scale. Proteogenomics is the integration of these data with the genome. Mining microbial proteomes allows validation of predicted orphan genes and correctio...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2009.03.005
更新日期：2009-06-01 00:00:00
abstract：:One current challenge in metabolic systems biology is to map out the regulation networks that control metabolism. From progress in this area, we conclude that non-transcriptional mechanisms (e.g. metabolite-protein interactions and protein phosphorylation) are highly relevant in actually controlling metabolic function...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2010.02.005
更新日期：2010-06-01 00:00:00
abstract：:The acquisition of drug resistance by Plasmodium falciparum has severely curtailed global efforts to control malaria. Our ability to define resistance has been greatly enhanced by recent advances in Plasmodium genetics and genomics. Sequencing and microarray studies have identified thousands of polymorphisms in the P....
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2007.07.007
更新日期：2007-08-01 00:00:00
abstract：:The small nucleotides pppGpp and ppGpp (or (p)ppGpp) are rapidly synthesized in response to nutritional stress. In Escherichia coli, the enzymes RelA and SpoT are triggered by different starvation signals to produce (p)ppGpp. In many Gram-positive bacteria this is carried out by RelA and two small homologs. (p)ppGpp, ...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2008.02.001
更新日期：2008-04-01 00:00:00
abstract：:The quest for the discovery of novel natural products has entered a new chapter with the enormous wealth of genetic data that is now available. This information has been exploited by using whole-genome sequence mining to uncover cryptic pathways, or biosynthetic pathways for previously undetected metabolites. Alternat...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2006.04.002
更新日期：2006-06-01 00:00:00
abstract：:Membrane shape is functionally linked with many cellular processes. The limiting membrane of vacuoles containing Toxoplasma gondii and Plasmodium apicomplexan parasites lies at the host-parasite interface. This membrane comprises intra-vacuolar and extra-vacuolar tubulo-vesicular deformations, which influence host-par...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2017.11.013
更新日期：2017-12-01 00:00:00
abstract：:The eukaryotic unicellular pathogen Plasmodium falciparum tightly regulates gene expression, both during development and in adaptation to dynamic host environments. This regulation is evident in the mutually exclusive expression of members of clonally variant virulence multigene families. While epigenetic regulators h...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2014.06.013
更新日期：2014-08-01 00:00:00
abstract：:The Gram-positive pathogen Staphylococcus aureus poses a serious risk to public health due to its prevalence as a commensal organism, its ability to cause a multitude of diseases, and the increasing incidence of antibiotic resistant strains. S. aureus infects diverse niches within vertebrates despite being challenged ...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2011.11.004
更新日期：2012-02-01 00:00:00
abstract：:Human cytomegalovirus (HCMV) is a clinically significant herpes virus that maintains a lifelong infection in the host. HCMV infection of endothelial cells and macrophages plays an important role in the establishment of latency and persistence, which appears critical for the maintenance of HCMV within the host. HCMV in...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s1369-5274(02)00334-x
更新日期：2002-08-01 00:00:00
abstract：:Much of what we know about cytokinesis in bacteria has come from studies with Escherichia coli, and efforts to comprehensively understand this fundamental process in this organism continue to intensify. Major recent advances include in vitro assembly of a membrane-tethered version of FtsZ into contractile rings in lip...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2010.09.015
更新日期：2010-12-01 00:00:00
abstract：:Some of the most successful drugs in the antibiotic pharmacopeia are those that inhibit bacterial cell wall biosynthesis. However, the worldwide spread of bacterial antibiotic resistance has eroded the clinical efficacy of these drugs and the antibiotic pipeline continues to be lean as drug discovery programs struggle...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2015.07.013
更新日期：2015-10-01 00:00:00
abstract：:Endosymbiotic theory goes back over 100 years. It explains the similarity of chloroplasts and mitochondria to free-living prokaryotes by suggesting that the organelles arose from prokaryotes through (endo)symbiosis. Gene trees provide important evidence in favour of symbiotic theory at a coarse-grained level, but the ...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2014.09.008
更新日期：2014-12-01 00:00:00
abstract：:Proteomic studies are designed to yield either qualitative information on proteins (identification, distribution, posttranslational modifications, interactions, structure and function) or quantitative information (abundance, distribution within different localizations, temporal changes in abundance due to synthesis an...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2012.02.005
更新日期：2012-06-01 00:00:00
abstract：:Elimination of non-functional or unwanted proteins is critical for cell growth and regulation. In bacteria, ATP-dependent proteases target cytoplasmic proteins for degradation, contributing to both protein quality control and regulation of specific proteins, thus playing roles parallel to that of the proteasome in euk...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2013.01.002
更新日期：2013-04-01 00:00:00
abstract：:In the process of yeast budding, an aged and deteriorated mother cell gives rise to a youthful and pristine daughter cell. This remarkable event offers a tractable model system for identifying factors affecting life expectancy and it has been established that multiple aging factors operate in parallel. Herein, we will...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2014.02.003
更新日期：2014-04-01 00:00:00
abstract：:Environmental pathogenic fungi present a paradox in that they are virulent in animals without requiring animal hosts for replication or survival, a phenomenon we call 'ready-made' virulence. In the human pathogenic fungus Cryptococcus neoformans, the capacity for virulence in animals may originate from environmental s...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s1369-5274(03)00082-1
更新日期：2003-08-01 00:00:00
abstract：:Candida albicans is a regular member of the intestinal microbiota in the majority of the human population. This underscores C. albicans' adaptation to life in the intestine without inducing competitive interactions with other microbes, or immune responses detrimental to its survival. However, specific conditions such ...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2020.05.006
更新日期：2020-08-01 00:00:00
abstract：:There has been a dramatic increase in the number of completely sequenced bacterial genomes during the past two years as a result of the efforts both of public genome agencies and the pharmaceutical industry. The availability of completely sequenced genomes permits more systematic analyses of genes, evolution and genom...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s1369-5274(98)80039-8
更新日期：1998-06-01 00:00:00
abstract：:Molecular phylogenetic studies reveal that many endosymbioses between bacteria and invertebrate hosts result from ancient infections followed by strict vertical transmission within host lineages. Endosymbionts display a distinctive constellation of genetic properties including AT-biased base composition, accelerated s...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s1369-5274(00)00088-6
更新日期：2000-06-01 00:00:00
abstract：:Antisense technology has been widely used to regulate gene expression. A tetracycline (tet)-regulated antisense-RNA-expressing system has been developed and used to downregulate chromosomally derived genes expressed in Staphylococcus aureus. This downregulation subsequently provides an evaluation of the virulence fact...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s1369-5274(02)00315-6
更新日期：2002-06-01 00:00:00
abstract：:DNA translocases play important roles during the bacterial cell cycle and in cell differentiation. Escherichia coli cells contain a multifunctional translocase, FtsK, which is involved in cell division, late steps of chromosome segregation and dimer resolution. In Gram-positive bacteria, the latter two processes are a...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2011.10.004
更新日期：2011-12-01 00:00:00
abstract：:Successful execution of differentiation programs requires cells to assess multitudes of internal and external cues and respond with appropriate gene expression programs. Here, we review how Bacillus subtilis sporulation network deals with these tasks focusing on the lessons generalizable to other systems. With feedfor...
journal_title：Current opinion in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.mib.2016.07.011
更新日期：2016-12-01 00:00:00
