A novel filtration-based processing method of liquid cytology specimens for human papillomavirus DNA testing by hybrid capture II.

Abstract:

:We evaluated a more efficient method of processing liquid-based cervical cytology specimens for human papillomavirus (HPV) DNA testing by Hybrid Capture II (HCII). Aliquots were made from 701 specimens in the following sequence: 4.0, 2.0, 1.0, 0.5, and 1.5 mL. The 4.0-mL aliquot was processed by the standard method (STP), and half of the processed material was tested by HCII. Other aliquots were processed with a new, filtration-based processing method (NPM). The 2.0-mL NPM aliquot had HCII test performance most similar to the STP, ie, similar HCII positivity (P = .4) and good test agreement (kappa = 0.85, 95% confidence interval [CI], 0.80-0.89). The 194 cytologic negatives had greater positivity by STP (P = .04) compared with the 2.0-mL aliquot processed by NPM; between-method agreement was modest (kappa = 0.54, 95% CI, 0.36-0.72). A lower positive cut point for the 2.0-mL NPM aliquot partially abrogated this minor difference. In 241 specimens diagnosed as low-grade and 31 as high-grade squamous intraepithelial lesions, there were no significant differences in HPVpositivity (>85% and 90%, respectively) between STP and NPM. NPM reduces specimen handling and decreases total testing time by approximately 33% without significant losses in HCII test performance.

journal_name

Am J Clin Pathol

authors

Castle PE,Garcia-Meijide M,Holladay EB,Chuke R,Payne J,Long A,Siefers H,Demuth F,Lorincz AT

subject

Has Abstract

pub_date

2005-02-01 00:00:00

pages

250-5

issue

2

eissn

0002-9173

issn

1943-7722

journal_volume

123

pub_type

杂志文章
  • Laboratory measurements of the oral direct factor Xa inhibitor edoxaban: comparison of prothrombin time, activated partial thromboplastin time, and thrombin generation assay.

    abstract:OBJECTIVES:Edoxaban, an oral direct factor Xa inhibitor, does not require routine monitoring. However, assessment of the anticoagulant effects may be required in certain situations. METHODS:We investigated the effects of edoxaban on prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin gene...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPQ2NJD3PXFTUG

    authors: Morishima Y,Kamisato C

    更新日期:2015-02-01 00:00:00

  • Determination of VH Family Usage in B-Cell Malignancies via the BIOMED-2 IGH PCR Clonality Assay.

    abstract:Objectives:To determine whether V H family usage in B-cell lymphoproliferative disorders can be deduced from polymerase chain reaction (PCR) product-length information obtained through the BIOMED-2 (Invivoscribe, San Diego, CA) clonality assay. Methods:We develop an algorithm that uses the sizing information of the BI...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqx007

    authors: McDonald TJ,Kuo L,Kuo FC

    更新日期:2017-06-01 00:00:00

  • The autopsy. Simple thoughts about the public needs and how to address them.

    abstract::Dialogue regarding the autopsy has been sustained largely by medical students and professionals, and by families of deceased patients. Exclusion of the general public may represent a significant reason for the inexorable decline of autopsy rates and politicoeconomic support. Sensitivity to public concerns regarding th...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:

    authors: McManus BM,Wood SM

    更新日期:1996-10-01 00:00:00

  • Comparative study of spleen pathology in drug abusers with thrombocytopenia related to human immunodeficiency virus infection and in patients with idiopathic thrombocytopenic purpura. A morphometric, immunohistochemical, and ultrastructural study.

    abstract::A morphometric and immunohistochemical study was performed to assess the spleen's alterations in patients with autoimmune thrombocytopenia and in drug abusers with thrombocytopenia (DAT) related to human immunodeficiency virus (HIV) infection. A total of 34 patients were included in the study: 20 DAT patients and 14 w...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/100.6.633

    authors: Marti M,Feliu E,Campo E,Palacin A,Berga L,Casals FJ,Urbano Ispizua A,Cardesa A,Rozman C

    更新日期:1993-12-01 00:00:00

  • Biospecimen Collection During the COVID-19 Pandemic.

    abstract:OBJECTIVES:Millions of biospecimens will be collected during the coronavirus disease 2019 (COVID-19) pandemic. As learned from severe acute respiratory syndrome (SARS), proper biospecimen handling is necessary to prevent laboratory-related infections. METHODS:Centers for Disease Control and Prevention and World Health...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1093/ajcp/aqaa171

    authors: Lankes HA,Makhlouf H

    更新日期:2021-01-04 00:00:00

  • Blood group substances as tumor antigens in the distal colon.

    abstract::Adenocarcinomas of the sigmoid colon and rectum were studied for the presence of blood group substances of the A, B, H system using the method of specific erythrocytic adherence. Of 17 adenocarcinomas studied, eight were found to have reactivity for blood group substances. All eight tumours showing reactivity for bloo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/69.6.594

    authors: Cooper HS,Haesler WE Jr

    更新日期:1978-06-01 00:00:00

  • Strategies for the analysis of oncogene overexpression. Studies of the neu oncogene in breast carcinoma.

    abstract::The development of a consistent strategy for the analysis of oncogene expression at the cellular level is essential for understanding the roles of these genes in the development and progression of human neoplasia. Detection of the neu oncogene products in breast carcinoma was selected as a model for analysis of oncoge...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/94.2.125

    authors: Naber SP,Tsutsumi Y,Yin S,Zolnay SA,Mobtaker H,Marks PJ,McKenzie SJ,DeLellis RA,Wolfe HJ

    更新日期:1990-08-01 00:00:00

  • Therapeutic control of oral anticoagulant treatment in The Netherlands.

    abstract::Therapeutic control achieved by an anticoagulant clinic may be assessed by calculation of the proportion of time spent by the patients within the prothrombin time target range aimed for by the clinic. Three calculation methods for such assessment have been described by other investigators. The three methods were compa...

    journal_title:American journal of clinical pathology

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1093/ajcp/90.6.685

    authors: van den Besselaar AM,van der Meer FJ,Gerrits-Drabbe CW

    更新日期:1988-12-01 00:00:00

  • Creatine kinase-MB: the journey to obsolescence.

    abstract:OBJECTIVES:To evaluate the clinical utility of and demand for the creatine kinase (CK)-MB assay. METHODS:We examined the number of CK-MB tests from 2007 through 2013 while we progressively deemphasized their use. We first removed CK-MB from the acute coronary syndrome (ACS) panel and then from the main menu and observ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPBIK3G4BWEJKO

    authors: Singh G,Baweja PS

    更新日期:2014-03-01 00:00:00

  • The antimicrobial removal device. A microbiological and clinical evaluation.

    abstract::A parallel study of blood cultured conventionally and following processing in an Antimicrobial Removal Device (ARD) was conducted with patients suspected of being clinically bacteremic. Cultures yielded 205 isolates from 87 clinically bacteremic patients, of whom 28 were receiving antibiotics at the time their culture...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/78.2.173

    authors: Wright AJ,Thompson RL,McLimans CA,Wilson WR,Washington JA 2nd

    更新日期:1982-08-01 00:00:00

  • Congenital hemophagocytic reticulosis.

    abstract::A fatal case of an apparently congenital form of hemophagocytic reticulosis is reported. The onset was manifested by hyperbilirubinemia and hepatosplenomegaly which were present at birth and persisted throughout life. Fever, anemia and pancytopenia developed at 1 month of age and became progressively worse. A splenect...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/65.4.495

    authors: Koto A,Morecki R,Santorineou M

    更新日期:1976-04-01 00:00:00

  • Detection of methionine in pernicious anemia megaloblasts and other types of erythroid precursors.

    abstract::Utilizing a bacterial-agar overlay technic incorporating the methionine-requiring bacterium Leukonostoc mesenteroides, little or no bacterial growth was seen surrounding the megaloblasts and proerythroblasts of eight patients who had severe untreated pernicious anemia. Similarly, scant bacterial growth was observed in...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/65.4.504

    authors: Kass L

    更新日期:1976-04-01 00:00:00

  • Comparison of disagreement and amendment rates by tissue type and diagnosis: identifying cases for directed blinded review.

    abstract::We sought to determine whether a group of cases that was relatively high in disagreements and subsequent amendments could be identified and targeted for blinded review. During a 4-year period, 8,916 surgical pathology and nongynecologic cytology cases were subjected to blinded review; of these, there were 616 disagree...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/DH4U-0KT6-QB01-KR55

    authors: Renshaw AA,Gould EW

    更新日期:2006-11-01 00:00:00

  • Potential use of monoclonal antibodies in the diagnostic distinction of gynecomastia from breast carcinoma in men.

    abstract::Immunohistochemical (IHC) assays using the monoclonal antibodies (MoAbs) B72.3 and B6.2, recognizing two distinct and independently expressed breast tumor-associated antigens (BTAAs), recently have been shown to significantly improve the accuracy of cytodiagnosis of breast nodules by fine-needle aspiration (FNA). To e...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.2.233

    authors: Mottolese M,Bigotti G,Coli A,Vitucci C,Natali PG

    更新日期:1991-08-01 00:00:00

  • A collaborative approach to lean laboratory workstation design reduces wasted technologist travel.

    abstract::Lean methodologies have been applied in many industries to reduce waste. We applied Lean techniques to redesign laboratory workstations with the aim of reducing the number of times employees must leave their workstations to complete their tasks. At baseline in 68 workflows (aggregates or sequence of process steps) stu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPE0PI2ENWYWMU

    authors: Yerian LM,Seestadt JA,Gomez ER,Marchant KK

    更新日期:2012-08-01 00:00:00

  • Basal cell (monomorphic) and minimally pleomorphic adenomas of the salivary glands. Distinction from the solid (anaplastic) type of adenoid cystic carcinoma in fine-needle aspiration.

    abstract::Cytologic features of the cell-stroma interface are useful in distinguishing between monomorphic adenomas of the basal cell type and adenoid cystic carcinoma. In basal cell adenomas, the collagenous stroma interdigitates with adjacent cells, whereas in adenoid cystic carcinoma, the two are separated by a sharp smooth ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1093/ajcp/106.1.35

    authors: Stanley MW,Horwitz CA,Rollins SD,Powers CN,Bardales RH,Korourain S,Stern SJ

    更新日期:1996-07-01 00:00:00

  • Measuring HER-2 in breast cancer. Immunohistochemistry, FISH, or ELISA?

    abstract::Measuring HER-2 is important for selecting optimal therapy and predicting prognosis in breast cancer patients. Current methods for evaluating HER-2 include measuring protein overexpression by immunohistochemistry (IHC), measuring gene copy number by fluorescent in situ hybridization (FISH), and measuring shed antigen ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/D76M-QEGU-62BT-HKER

    authors: Yeh IT

    更新日期:2002-06-01 00:00:00

  • Lack of HER2 overexpression and amplification in small intestinal adenocarcinoma.

    abstract::HER2 overexpression and amplification have been studied as a therapeutic and prognostic target in a number of human cancers, including esophageal, gastric, and colorectal adenocarcinomas. However, HER2 status has not been well investigated in primary small intestinal adenocarcinoma, probably because of its rarity. In ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPK6QHNNOEMJIM

    authors: Chan OT,Chen ZM,Chung F,Kawachi K,Phan DC,Himmelfarb E,Lin F,Perry A,Wang HL

    更新日期:2010-12-01 00:00:00

  • Effects of chemotherapy on pathologic and biologic characteristics of locally advanced breast cancer.

    abstract::In 42 patients with locally advanced breast cancer treated with neoadjuvant chemotherapy followed by surgery and radiation therapy, the effects of chemotherapy on tumor architecture, morphometric nuclear and nucleolar characteristics, DNA ploidy, proliferation index measured by mitotic activity index, expression of di...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/107.2.211

    authors: Honkoop AH,Pinedo HM,De Jong JS,Verheul HM,Linn SC,Hoekman K,Wagstaff J,van Diest PJ

    更新日期:1997-02-01 00:00:00

  • Babesiosis: problems in diagnosis using autoanalyzers.

    abstract::A 76-year-old white man previous diagnosed as having Waldenstrom's macroglobulinemia continued with persistent fevers and sweats for two and a half years. Recently, repeated automated differentials during 11 days of hospitalization failed to note any intracellular inclusions in the RBCs. Blood sent to the Microbiology...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/83.4.520

    authors: Bruckner DA,Garcia LS,Shimizu RY,Goldstein EJ,Murray PM,Lazar GS

    更新日期:1985-04-01 00:00:00

  • Chronic myelogenous leukemia, BCR-ABL1+.

    abstract::Session 1 of the 2007 Workshop of the Society for Hematopathology/European Association for Haematopathology focused on chronic myelogenous leukemia, BCR-ABL1+ (CML). CML is a myeloproliferative neoplasm arising at the level of a pluripotent stem cell and consistently associated with the BCR-ABL1 fusion gene. CML most ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/AJCPUN89CXERVOVH

    authors: Vardiman JW

    更新日期:2009-08-01 00:00:00

  • A comparative marker study of large cell lymphoma, Hodgkin's disease, and true histiocytic lymphoma in paraffin-embedded tissue.

    abstract::A comparative study of large cell lymphoma (LCL) (ten B and ten T), Hodgkin's disease (15 cases), and true histiocytic lymphoma (two cases) was undertaken, using formalin-fixed paraffin-embedded tissue sections, a panel of eight antibodies, and one lectin to determine if any particular antibody or immunologic profile ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/86.5.591

    authors: Meis JM,Osborne BM,Butler JJ

    更新日期:1986-11-01 00:00:00

  • Essential thrombocythemia with acquired von Willebrand's disease.

    abstract::Two patients with essential thrombocythemia showed a marked decrease in ristocetin-induced platelet aggregation in addition to other aggregation defects during routine aggregation studies for prolonged bleeding times. Further investigation revealed type I von Willebrand's disease (vWD) in one patient and a variant of ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/88.1.102

    authors: Raman BK,Sawdyk M,Saeed SM

    更新日期:1987-07-01 00:00:00

  • Adenovirus in the gastrointestinal tracts of immunosuppressed patients.

    abstract::Adenovirus was cultured from various gastrointestinal sites and one lung specimen from seven immunosuppressed patients, four of whom were bone marrow transplant recipients. Histologic examination and in situ hybridization studies of the lung demonstrated adenovirus in the bronchial epithelium and alveolar lining cells...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/97.4.555

    authors: Strickler JG,Singleton TP,Copenhaver CM,Erice A,Snover DC

    更新日期:1992-04-01 00:00:00

  • Direct and indirect immunofluorescence as a diagnostic adjunct in the interpretation of nonneoplastic medical lung disease.

    abstract::Fresh open lung biopsy material from 57 patients was incubated with fluoresceinated complement and immunoglobulin antisera. An indirect immunofluorescent assay using neonatal lung as substrate was conducted as well. Direct immunofluorescent patterns could be categorized into interalveolar septal capillary deposition, ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/EHUQ-HW6K-0G41-E0DX

    authors: Magro CM,Morrison C,Pope-Harman A,Rothrauff SK,Ross P Jr

    更新日期:2003-02-01 00:00:00

  • Fine-needle aspiration cytologic diagnosis of Kikuchi's lymphadenitis. A report of 27 cases.

    abstract::Kikuchi's lymphadenitis is a self-limiting condition typically affecting young patients. Surgical biopsy is unnecessary if a firm diagnosis can be rendered by fine-needle aspiration cytology (FNAC). The authors report the FNAC findings for 27 cases, including 24 female and 3 male patients aged 12 to 43 years. Histolog...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/102.4.454

    authors: Tsang WY,Chan JK

    更新日期:1994-10-01 00:00:00

  • Anti-glomerular basement membrane glomerulonephritis: a morphologic study of 80 cases.

    abstract::Anti-glomerular basement membrane (anti-GBM) glomerulonephritis is a rare disease caused by IgG autoantibodies against the glomerular basement membrane. We describe clinical and pathologic findings in a series of renal biopsy specimens from 80 patients. The patients ranged in age from 16 to 87 years. The age distribut...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/nptp-4ukv-7ju3-elmq

    authors: Fischer EG,Lager DJ

    更新日期:2006-03-01 00:00:00

  • Image cytometric measurement of nuclear proliferation markers (MIB-1, PCNA) in astrocytomas. Prognostic significance.

    abstract::Proliferative activity in astrocytomas, measured by image cytometry, was related to prognosis. Fifty-eight astrocytic neoplasms (grade 1 or 2, 11; grade 3, 31; glioblastoma multiforme, 16) were immunostained for Ki-67 (MIB-1; 1:50) and proliferating cell nuclear antigen (PCNA; prediluted). Proliferative activity (nucl...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/109.1.69

    authors: Kirkegaard LJ,DeRose PB,Yao B,Cohen C

    更新日期:1998-01-01 00:00:00

  • Real-Time Clinical Decision Support Decreases Inappropriate Plasma Transfusion.

    abstract:Objectives:To curtail inappropriate plasma transfusions, we instituted clinical decision support as an alert upon order entry if the patient's recent international normalized ratio (INR) was 1.7 or less. Methods:The alert was suppressed for massive transfusion and within operative or apheresis settings. The plasma ord...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqx061

    authors: Shah N,Baker SA,Spain D,Shieh L,Shepard J,Hadhazy E,Maggio P,Goodnough LT

    更新日期:2017-08-01 00:00:00

  • The expression of HLA class I antigens in germ cell testicular cancer.

    abstract::The expression of HLA class I antigens in testicular germ cell tumors (TGCTs) was studied by the immunoperoxidase technique. In the normal testicle, the interstitial cells of Leydig as well as most of the germ cells were significantly stained. In typical seminoma, 75% of the tumor cells in stage I and 30% in stage II ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/93.2.202

    authors: Klein B,Klein T,Konichezky M,Nyska A,Livini E,Levine I,Zamir R,Kooperman O,Lurie H

    更新日期:1990-02-01 00:00:00