Identification of addicsin/GTRAP3-18 as a chronic morphine-augmented gene in amygdala.

Abstract:

:Using subtractive cloning, we identified a 1.4 kb mRNA that was ubiquitously expressed in various tissues; this mRNA was highly up-regulated in amygdala nuclei in mice when morphine was repeatedly administered but not when an opiate-receptor antagonist was co-administered. The mRNA encodes a 23 kDa protein, designated 'addicsin'. This contains two putative PKC-phosphorylation motifs and several hydrophobic regions, and was recovered in a soluble protein fraction of brain lysate. Its primary structure showed 98% identity with that of rat glutamate-transporter-associated protein 3-18 (GTRAP3-18), a putative modulator of neural glutamate-transporter EAAC1. Up-regulation of addicsin expression by morphine may affect glutamate uptake in the amygdala, causing mice to develop morphine tolerance and dependence.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Ikemoto MJ,Inoue K,Akiduki S,Osugi T,Imamura T,Ishida N,Ohtomi M

doi

10.1097/00001756-200211150-00018

subject

Has Abstract

pub_date

2002-11-15 00:00:00

pages

2079-84

issue

16

eissn

0959-4965

issn

1473-558X

journal_volume

13

pub_type

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