Alterations induced by E-cadherin and beta-catenin antibodies during the development of Bufo arenarum (Anura-Bufonidae).

Abstract:

:E(epithelial)-cadherin is a member of a calcium-dependent family of cell surface glycoproteins involved in cell-cell adhesion and morphogenesis. Catenins are a large family of proteins that connect the cadherins to the cytoskeleton. They are important for cadherin function and for transducing signals involved in specification of cell fate during embryogenesis. The best characterized catenins include alpha-, beta-, gamma-, and p120-catenin. Using specific antibodies, we studied the expression and distribution of E-cadherin, and alpha- and beta-catenin in developmental stages of Bufo arenarum toad. The three proteins were found co-localized in stages 19 to 41 of development. Surprisingly, E-cadherin was the only of these three proteins found earlier than stage 19. To test whether E-cadherin and beta-catenin have a functional role in Bufo arenarum embryogenesis, stage 17 whole embryos were incubated with anti-E-cadherin and beta-catenin antibodies. Both anti-E-cadherin and anti-beta-catenin antibodies induced severe morphological alterations. However, while alterations produced by the anti-beta-catenin antibody, showed some variability from the most severe (neural tube and notochord duplication) to a simple delay in development, the alterations with anti-E-cadherin were homogeneous. These observations suggest a critical role for E-cadherin and beta-catenin in the early embryonic development of the Bufo arenarum toad. Our results are consistent with the developmental role of these proteins in other species. One of the most surprising findings was the blockage with the anti-beta-catenin antibodies on later embryo stages, and we hypothesize that the partial axes duplication could be mediated by the notochord induction.

journal_name

Histol Histopathol

authors

Izaguirre MF,Adur JF,Soler AP,Casco VH

doi

10.14670/HH-16.1097

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

1097-106

issue

4

eissn

0213-3911

issn

1699-5848

journal_volume

16

pub_type

杂志文章
  • Histology and ultrastructure of the pineal organ in the domestic goose.

    abstract::The pineal organs of 14-week-old domestic geese were investigated with light and electron microscopy. The pineals consisted of a wide distal part and a narrow middle-proximal one. The glands were attached to the intercommissural region via the choroid plexus. The pineal parenchyma was formed by round or elongated foll...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-21.1075

    authors: Prusik M,Lewczuk B,Nowicki M,Przybylska-Gornowicz B

    更新日期:2006-10-01 00:00:00

  • Aluminium exposure induces Alzheimer's disease-like histopathological alterations in mouse brain.

    abstract::Aluminium (Al) is a neurotoxic metal and Al exposure may be a factor in the aetiology of various neurodegenerative diseases such as Alzheimer's disease (AD). The major pathohistological findings in the AD brain are the presence of neuritic plaques containing beta-amyloid (Abeta) which may interfere with neuronal commu...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-23.433

    authors: Rodella LF,Ricci F,Borsani E,Stacchiotti A,Foglio E,Favero G,Rezzani R,Mariani C,Bianchi R

    更新日期:2008-04-01 00:00:00

  • Bone marrow histopathology in chronic myelogenous leukemia (CML)--evaluation of distinctive features with clinical impact.

    abstract::Bone marrow features in stable-phase chronic myelogenous leukemia (CML) are characterized by a striking heterogeneity which is determinable by appropriate means including representative pre-treatment trephine biopsies, immunohistochemistry and morphometry. Cell lineages involved to a variable extent consist not only o...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-14.1241

    authors: Thiele J,Kvasnicka HM,Fischer R

    更新日期:1999-10-01 00:00:00

  • Antigen retrieval on epoxy sections based on tissue infiltration with a moderately increased amount of accelerator to detect immune complex deposits in glomerular tissue.

    abstract::We wanted to examine the effect of antigen retrieval on epoxy sections where the tissue had been infiltrated by resin containing moderately increased amounts of accelerator. The concentration of accelerator DMP-30 (Tri(Dimethyl Amino Methyl) Phenol) was varied in the range of 0% to 4% in the infiltration step of the t...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-14.151

    authors: Brorson SH,Andersen T,Haug S,Kristiansen I,Risstubben A,Tchou H,Ulstein J

    更新日期:1999-01-01 00:00:00

  • Expression pattern of the endoplasmic reticulum stress protein gp96 in monophasic and chronic relapsing form of experimental autoimmune encephalomyelitis in rats.

    abstract::Gp96 is the endoplasmic reticulum (ER)-resident molecular chaperone, which is involved in the correction of unfolded proteins, in the activation of proteasome-dependent ER-associated degradation of the misfolded proteins, and in activation of the protein translation that modulates polypeptide traffic into the ER. Furt...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-28.61

    authors: Jakovac H,Grebic D,Barac-Latas V,Mrakovcic I,Radosevic-Stasic B

    更新日期:2013-01-01 00:00:00

  • Interocular effect of intravitreal injection of 6-hydroxydopamine and dopamine on spinule formation in teleost retina.

    abstract::Terminal dendrites of cone horizontal cells (HCs) in teleost retinas show numerous spine-like protrusions named spinules, which are invaginated into the cone pedicles during light-adaptation, but retracted during dark-adaptation. Somata of HC show nematosomes whose size decreases as the number of spinules increases. M...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-17.721

    authors: García M,Grzywacz NM,De Juan J

    更新日期:2002-01-01 00:00:00

  • Close association of centroacinar/ductular and insular cells in the rat pancreas.

    abstract::Close contacts between endocrine insular cells and exocrine acinar, centroacinar and ductular cells occur frequently in the rat pancreas as seen by both light and electron microscopy. Islets of Langerhans are surrounded incompletely by a thin connective tissue capsule or mantle but numerous exocrine-endocrine cell con...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Leeson TS,Leeson R

    更新日期:1986-01-01 00:00:00

  • High-grade renal cell carcinoma with emperipolesis: Clinicopathological, immunohistochemical and molecular-genetic analysis of 14 cases.

    abstract::Emperipolesis has recently been described as a constant feature of "biphasic squamoid" papillary renal cell carcinoma (BPRCC). We also noticed this in some high-grade (HG) RCC, which promoted the present study to estimate the incidence of emperipolesis in RCCs and to describe them in further detail. 14 cases of HGRCC ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-11-925

    authors: Rotterova P,Martinek P,Alaghehbandan R,Prochazkova K,Damjanov I,Rogala J,Suster S,Perez-Montiel D,Alvarado-Cabrero I,Sperga M,Svajdler M,Michalova K,Pivovarcikova K,Daum O,Hora M,Dusek M,Ondic O,Stehlikova A,Michal M

    更新日期:2018-03-01 00:00:00

  • Adhesion-dependent signalling and the initiation of haemostasis and thrombosis.

    abstract::Cell-cell and cell-extracellular matrix adhesion are critical aspects of platelet function, regulating interactions between circulating platelets in the bloodstream with the blood vessel wall. In haemostasis, platelets adhere to the subendothelial matrix of a damaged vessel, spread over the surface and recruit additio...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-13.837

    authors: Andrews RK,Berndt MC

    更新日期:1998-07-01 00:00:00

  • Prognostic significance of PTK7 in human malignancies.

    abstract:BACKGROUND:Protein tyrosine kinase 7 (PTK7) is a member of receptor protein tyrosine kinase-like molecules, which is involved in tumorigenesis. However, the association between PTK7 expression and its pathological significance in survival prognosis remains under investigation. The purpose of this meta-analysis is to cl...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,meta分析

    doi:10.14670/HH-11-933

    authors: Chen G,Qi S,Yang X,Chen W

    更新日期:2018-04-01 00:00:00

  • An immunohistochemical study of NFE2L2, KEAP1 and 8-hydroxy-2'-deoxyguanosine and the EMT markers SNAI2, ZEB1 and TWIST1 in metastatic melanoma.

    abstract:BACKGROUND:Little is known regarding the role of redox balance regulators in metastatic melanomas, but there is some evidence for a link between epithelial-to-mesenchymal transition (EMT) and cellular redox status. METHODS:We compared the immunohistochemical expression of nuclear factor erythroid-2-related factor 2 (N...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-11-778

    authors: Hintsala HR,Haapasaari KM,Soini Y,Karihtala P

    更新日期:2017-02-01 00:00:00

  • Morphogenesis of normal human salivary gland cells in vitro.

    abstract::Primary cultured human salivary gland cells were transfected with ori-defective mutant DNA of SV40. Following 2-3 weeks of transfection, slowly expanding colonies consisting of small compact cells emerged, while mock-transfected cells did not grow any more and eventually entered crisis, followed by cell death. Using l...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:

    authors: Azuma M,Sato M

    更新日期:1994-10-01 00:00:00

  • The prognostic significance of thymidine phosphorylase, thymidylate synthase and dihydropyrimidine dehydrogenase mRNA expressions in breast carcinomas.

    abstract::Thymidine phosphorylase (TP), thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) have been indicated as possible predictive markers for epithelial malignancies. All these three enzymes are actively involved in 5-FU metabolism. In this report, we investigated mRNA expression of these factors with real-...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-19.129

    authors: Li H,Suo Z,Zhang Y,Risberg B,Karlsson MG,Villman K,Nesland JM

    更新日期:2004-01-01 00:00:00

  • Modulation of pulmonary neuroendocrine cells in idiopathic interstitial pneumonia.

    abstract::In order to reveal modulation of the number of pulmonary neuroendocrine cells (PNEC) in interstitial lung diseases and to clarify significance of cell proliferation activity in occurrence of PNEC, we counted airway PNEC of the patients of idiopathic interstitial pneumonia, secondary interstitial pneumonia and control ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-17.1121

    authors: Ito T,Ogura T,Ogawa N,Udaka N,Hayashi H,Inayama Y,Yazawa T,Kitamura H

    更新日期:2002-10-01 00:00:00

  • Targeting WNT, protein kinase B, and mitochondrial membrane integrity to foster cellular survival in the nervous system.

    abstract::Targeting essential cellular pathways that determine neuronal and vascular survival can foster a successful therapeutic platform for the treatment of a wide variety of degenerative disorders in the central nervous system. In particular, oxidative cellular injury can precipitate several nervous system disorders that ma...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-19.495

    authors: Chong ZZ,Maiese K

    更新日期:2004-04-01 00:00:00

  • Recent development of in vivo cryotechnique to cryobiopsy for living animals.

    abstract::Various microscopic methods have been used to analyze the morphology and molecular distribution of cells and tissues. Using conventional procedures, however, ischemic or anoxic artifacts are inevitably caused by tissue-resection or perfusion-fixation. The in vivo cryotechnique (IVCT) was developed to overcome these pr...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-22.1281

    authors: Ohno N,Terada N,Saitoh S,Zhou H,Fujii Y,Ohno S

    更新日期:2007-11-01 00:00:00

  • New insights into the cytoplasmic function of PML.

    abstract::PML is a tumour suppressor inactivated in Acute Promyelocytic Leukaemia (APL). PML is the essential component of a subnuclear structure called the PML nuclear body (PML-NB), which is disrupted in APL. By targeting different cellular proteins to this structure, PML can either hamper or potentiate their functions. The P...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-22.937

    authors: Salomoni P,Bellodi C

    更新日期:2007-08-01 00:00:00

  • Ewing sarcoma and the new emerging Ewing-like sarcomas: (CIC and BCOR-rearranged-sarcomas). A systematic review.

    abstract::Ewing-like sarcomas (ELS) are a heterogenous group of tumors that frequently affect pediatric and young adult patients. Accurate classification and distinction from the Ewing sarcoma family of tumor (ESFT) is decisive in patient management. ELS share a significant morphologic, immunohistochemical and clinical overlap ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-11-792

    authors: Machado I,Navarro S,Llombart-Bosch A

    更新日期:2016-11-01 00:00:00

  • The relative effects of severe burn injury and pre- and post-natal protein deprivation on mandibular condyle morphology.

    abstract::The mandible has a mixed embryological origin, and its growth is associated with the secondary cartilage of the condyle process (CP). In this area, growth depends on an array of intrinsic and extrinsic factors that influence protein metabolism. In the present study, we used an adolescent rat model to evaluate the grow...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-25.45

    authors: de Oliveira BC,de Oliveira F,Martini DT,Prisco CR,da Silva Riguetti MM,Liberti EA,de Campos Boldrini S

    更新日期:2010-01-01 00:00:00

  • Functional morphology of the equine pelvic flexure and its role in disease. A review.

    abstract::The hindgut is the major site in the horse for nutrient digestion and absorption. Most of this activity occurs in the large intestinal compartments, i.e., cecum, right and left ventral colon and left and right dorsal colon. The colonic pelvic flexure is a short and narrow loop connecting the left ventral and left dors...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-15.983

    authors: Lopes MA,Pfeiffer CJ

    更新日期:2000-07-01 00:00:00

  • Immunocytochemical localization of the vacuolar H(+)-ATPase pump in the kidney.

    abstract::In this article we review immunocytochemical localization studies using a monoclonal antibody raised against the 31 kD subunit of bovine H(+)-ATPase, and indirect immunofluorescent staining. In the proximal tubules there is intense H(+)-ATPase staining along the brush borders of S1 and S2, and linear subvillar invagin...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:

    authors: Bastani B

    更新日期:1997-07-01 00:00:00

  • Differential location and structural specificities of sialic acid-beta-D-Gal sequences belonging to sialoderivatives of rabbit oviduct under hormonal treatment.

    abstract::Sialoderivatives expressed in the rabbit oviduct under hormonal treatment have been investigated in situ by lectin histochemistry with a view to specifying further regional and temporal specializations which enable ampulla and isthmus to play distinct roles in the reproductive events. Application of MAL II and SNA lec...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-19.1175

    authors: Gabrielli MG,Bondi AM,Materazzi G,Menghi G

    更新日期:2004-10-01 00:00:00

  • Adipose-derived stem cells in articular cartilage regeneration: current concepts and optimization strategies.

    abstract::Knee osteoarthritis (KOA) is the most common progressive joint disorder associated with disability in the world. As a chronic disease, KOA has multifactorial etiology. However, the poor self-healing ability of the articular cartilage due to its intrinsic tissue hypovascularity and hypocellularity seems to be directly ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-11-955

    authors: Gu X,Li C,Yin F,Yang G

    更新日期:2018-07-01 00:00:00

  • Might the Masson trichrome stain be considered a useful method for categorizing experimental tendon lesions?

    abstract::Strain injuries of tendons are the most common orthopedic injuries in athletic subjects, be they equine or human. When the tendon is suddenly damaged, an acute inflammatory phase occurs whereas its repetitive overloading may cause chronic injuries. Currently the criteria used for grading injuries are general and subje...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-11-601

    authors: Martinello T,Pascoli F,Caporale G,Perazzi A,Iacopetti I,Patruno M

    更新日期:2015-08-01 00:00:00

  • Distribution of bile acid receptor TGR5 in the gastrointestinal tract of dogs.

    abstract::Takeda-G-protein-receptor-5 (TGR5) is a receptor for bile acids and its expression has been described in a variety of tissues and species. Characterization of TGR5 distribution and function has been investigated in drug discovery for the treatment of metabolic diseases in humans. Because dogs are one of the species us...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-18-025

    authors: Giaretta PR,Suchodolski JS,Blick AK,Steiner JM,Lidbury JA,Rech RR

    更新日期:2019-01-01 00:00:00

  • Daunorubicin does not induce immunohistochemically detectable endothelial dysfunction in rabbit aorta and femoral artery.

    abstract::Anthracyclines are one of the most effective anticancer drugs ever developed, but their clinical use has been hampered by the risk of severe cardiotoxicity. In this study, we investigated whether rabbits exposed to a different cumulative dose of anthracycline suffer from immunohistochemically detectable vascular toxic...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-26.551

    authors: Nachtigal P,Sterba M,Popelová O,Večeřová L,Kudláčková Z,Jirkovský E,Geršl V

    更新日期:2011-05-01 00:00:00

  • Reduced expression of the membrane skeleton protein beta1-spectrin (SPTBN1) is associated with worsened prognosis in pancreatic cancer.

    abstract::Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. In addition, spectrins have been implicated in TGF-beta signaling. The aim of the present study was to analyze the expression and localization of beta...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-25.1497

    authors: Jiang X,Gillen S,Esposito I,Giese NA,Michalski CW,Friess H,Kleeff J

    更新日期:2010-12-01 00:00:00

  • Dietary aluminium and renal failure in the koala (Phascolarctos cinereus).

    abstract::The study investigated the link between the potentially nephrotoxic levels of aluminium ingested in the natural diet of eucalypt leaves by koalas in the Adelaide Hills, South Australia, and the high incidence of renal failure in koalas within this habitat. Routine histology of kidney specimens revealed no pathologies ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-19.777

    authors: Haynes JI,Askew MJ,Leigh C

    更新日期:2004-07-01 00:00:00

  • The relationship between mucin phenotype and clinicopathological factors, proliferative activity and p53 expression in gastric hyperplastic polyps.

    abstract:AIMS:Gastric hyperplastic polyps (GHPs) are the most common polypoid lesion of the stomach, and their malignant potential has been demonstrated. In the present study, we evaluated the mucin phenotypes of GHPs and investigated the relationships among mucin phenotypes and clinical-pathological factors, proliferative acti...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-29.343

    authors: Li J,Wu LH,Shi Y,Li HM,Song MY,Jiao YF

    更新日期:2014-03-01 00:00:00

  • Regulation and function of sphingosine kinase 2 in diseases.

    abstract::Sphingosine kinase functions to phosphorylate sphingosine to sphingosine 1-phosphate (S1P) to keep balance in the metabolites of sphingolipids. There are two isoforms of sphingosine kinase, sphingosine kinase 1 (SphK1) and sphingosine kinase 2 (SphK2). Although SphK1 and SphK2 share high sequence similarity, SphK2 has...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-11-939

    authors: Song DD,Zhou JH,Sheng R

    更新日期:2018-05-01 00:00:00