Abstract:
:Wnt-4 signaling plays a critical role in kidney development and is associated with the epithelial conversion of the metanephric mesenchyme. Furthermore, secreted Frizzled-related proteins (sFRPs) that can bind Wnts are normally expressed in the developing metanephros, and function in other systems as modulators of Wnt signaling. sfrp-1 is distributed throughout the medullary and cortical stroma in the metanephros, but is absent from condensed mesenchyme and primitive tubular epithelia of the developing nephron where wnt-4 is highly expressed. In contrast, sfrp-2 is expressed in primitive tubules. To determine their role in kidney development, recombinant sFRP-1, sFRP-2 or combinations of both were applied to cultures of 13-dpc rat metanephroi. Both tubule formation and bud branching were markedly inhibited by sFRP-1, but concurrent sFRP-2 treatment restored some tubular differentiation and bud branching. sFRP-2 itself showed no effect on cultures of metanephroi. In cultures of isolated, induced rat metanephric mesenchymes, sFRP-1 blocked events associated with epithelial conversion (tubulogenesis and expression of lim-1, sfrp-2 and E-cadherin); however, it had no demonstrable effect on early events (compaction of mesenchyme and expression of wt1). As shown herein, sFRP-1 binds Wnt-4 with considerable avidity and inhibits the DNA-binding activity of TCF, an effector of Wnt signaling, while sFRP-2 had no effect on TCF activation. These observations suggest that sFRP-1 and sFRP-2 compete locally to regulate Wnt signaling during renal organogenesis. The antagonistic effect of sFRP-1 may be important either in preventing inappropriate development within differentiated areas of the medulla or in maintaining a population of cortical blastemal cells to facilitate further renal expansion. On the other hand, sFRP-2 might promote tubule formation by permitting Wnt-4 signaling in the presence of sFRP-1.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Yoshino K,Rubin JS,Higinbotham KG,Uren A,Anest V,Plisov SY,Perantoni AOdoi
10.1016/s0925-4773(01)00282-9subject
Has Abstractpub_date
2001-04-01 00:00:00pages
45-55issue
1-2eissn
0925-4773issn
1872-6356pii
S0925477301002829journal_volume
102pub_type
杂志文章abstract::BMP2, like its Drosophila homologue dpp, is an important signaling molecule for specification of cardiogenic mesoderm in vertebrates. Here, we analyzed the time-course of BMP2-requirement for early heart formation in whole chick embryos and in explants of antero-lateral plate mesoderm. Addition of Noggin to explants i...
journal_title:Mechanisms of development
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abstract::Nuclear translocation, driven by the motility apparatus consisting of the cytoplasmic dynein motor and microtubules, is essential for cell migration during embryonic development. Bicaudal-D (Bic-D), an evolutionarily conserved dynein-interacting protein, is required for developmental control of nuclear migration in Dr...
journal_title:Mechanisms of development
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abstract::The mouse and human Hox complex consists of 39 genes in four linkage groups (A-D). Although the structure and expression patterns of most of these genes have been reported, the 5' members of the Hox C linkage group have been only partially characterized. Herein we report the primary and genomic structure of the mouse ...
journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
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abstract::Increases in Na/K-ATPase activity occur concurrently with the onset of cavitation and are associated with increases in Na(+)-pump subunit mRNA and protein expression. We have hypothesized that the alpha1-isozyme of the Na/K-ATPase is required to mediate blastocyst formation. We have tested this hypothesis by character...
journal_title:Mechanisms of development
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doi:10.1016/j.mod.2004.04.005
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
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abstract::The vascularization of the central nervous system occurs by angiogenic sprouting, a process in which different factors like vascular endothelial growth factor (VEGF) and angiopoietin (Ang)-1/2 must act in a coordinated fashion. We investigated how these factors participate in capillarization of the cerebellum, an area...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00471-3
更新日期:2001-10-01 00:00:00
abstract::We present a complete staging table of normal development for the lungless salamander, Hemidactylium scutatum (Caudata: Plethodontidae). Terrestrial egg clutches from naturally ovipositing females were collected and maintained at 15 °C in the laboratory. Observations, photographs, and time-lapse movies of embryos were...
journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2015-05-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(02)00451-3
更新日期:2003-03-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(03)00041-8
更新日期:2003-05-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00513-5
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pub_type: 杂志文章,评审
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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更新日期:2006-11-01 00:00:00
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journal_title:Mechanisms of development
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更新日期:2010-07-01 00:00:00
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
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