Abstract:
:The intercalation of mesenchymal cells into epithelia, through mesenchymal-to-epithelial transition (MET), underlies organogenesis, for example, in nephrogenesis, and tissue regeneration, during cell renewal and wound repair. Despite its importance, surprisingly little is known about the mechanisms that bring about MET in comparison with the related and much-studied, reverse process, epithelial-to-mesenchymal transition (EMT). We analyse the molecular events that regulate MET as stellate cells integrate into the established epithelium of the developing renal tubules in Drosophila. We show that stellate cells polarise as they integrate between epithelial principal cells and that the normal, localised expression of cell polarity proteins in principal cells is required for stellate cells to become epithelial. While the basolateral and apical membranes act as cues for stellate cell polarity, adherens junction integrity is required to regulate their movement through the epithelium; in the absence of these junctions stellate cells continue migrating into the tubule lumen. We also show that expression of basolateral proteins in stellate cells is a prerequisite for their ingression between principal cells. We present a model in which the contacts with successive principal cell membrane domains made by stellate cells as they integrate between them act as a cue for the elaboration of stellate cell polarity. We suggest that the formation of zonula adherens junctions between new cell neighbours establishes their apico-basal positions and stabilises them in the epithelium.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Campbell K,Casanova J,Skaer Hdoi
10.1016/j.mod.2010.04.002subject
Has Abstractpub_date
2010-07-01 00:00:00pages
345-57issue
7-8eissn
0925-4773issn
1872-6356pii
S0925-4773(10)00025-0journal_volume
127pub_type
杂志文章abstract::The vertebrate neural tube develops by two distinct mechanisms. Anteriorly, in the brain and future trunk (cervicothoracic) region, 'primary neurulation' occurs, where an epithelial sheet rolls or bends into a tube. Posteriorly, in the future lumbar and tail region, the neural tube forms by 'secondary neurulation', wh...
journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/j.mod.2004.04.022
更新日期:2004-10-01 00:00:00
abstract::We have identified a novel chicken gene, cMespo, which encodes a basic-helix-loop-helix (bHLH) protein with sequence homology to a subgroup of bHLH transcription factors that have been implicated in somitogenesis. cMespo transcripts are first found in the primitive streak of gastrulating chick embryos (HH stage 4) and...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00424-x
更新日期:2000-10-01 00:00:00
abstract::The chick dermis is known to control the formation of feathers and interfeathery skin in a hexagonal pattern. The evidence that the segregation of two types of fibroblasts involves Delta/Notch signalling is based on three facts. Rings of C-Delta-1-expressing fibroblasts precede and delimit the forming feather primordi...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00027-6
更新日期:1998-03-01 00:00:00
abstract::Dickkopf-1 (dkk-1) is member of a novel family of secreted proteins and functions in head induction during Xenopus embryogenesis, acting as a potent inhibitor of Wnt signalling. Here we report: (1) the isolation of two additional murine members of the dkk family, dkk-2 and dkk-3; and (2) analysis of adult and embryoni...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00138-0
更新日期:1999-09-01 00:00:00
abstract::Tribolium castaneum is a well-characterised model insect, whose short germ-band mode of embryonic development is characteristic of many insect species and differs from the exhaustively studied Drosophila. Mechanisms of early neurogenesis, however, show significant conservation with Drosophila, as a characteristic patt...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2013.02.003
更新日期:2013-04-01 00:00:00
abstract::The normal embryogenesis of marine animals is typically confined to a species-specific range of temperatures. Within that temperature range development results in a consistent, or canalized, phenotype, whereas above and below the range abnormal phenotypes are produced. This study reveals a high temperature threshold, ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2019.04.002
更新日期:2019-06-01 00:00:00
abstract::Cell fate specification during inner ear development is dependent upon regional gene expression within the otic vesicle. One of the earliest cell fate determination steps in this system is the specification of neural precursors, and regulators of this process include the Atonal-related basic helix-loop-helix genes, Ng...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2004.12.011
更新日期:2005-05-01 00:00:00
abstract::The gastrointestinal tract is subdivided into regions with different roles in digestion and absorption. How this patterning is established is unknown. We now report that the pancreatic-duodenal homeobox 1 gene (pdx1) is also expressed in cells of the distal stomach. Positive cells include subpopulations of the three m...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(96)00609-0
更新日期:1996-12-01 00:00:00
abstract::The Ras protein activates at least three different pathways during early development. Two of them regulate mesodermal gene expression and the third is thought to participate in the control of actin cytoskeleton dynamics via the Ral protein. From a yeast two-hybrid screen of a Xenopus maternal cDNA library, we identifi...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2004.07.008
更新日期:2004-12-01 00:00:00
abstract::Germ-line mutations of LKB1 and PTEN tumor suppressor genes underlie the phenotypically related Peutz-Jeghers syndrome (PJS) and Cowden disease (CD), respectively. To analyze possible developmental roles of PTEN and LKB1, we have studied their mRNA expression during mouse embryonic development (E7-17.5) by in situ hyb...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00050-7
更新日期:1999-05-01 00:00:00
abstract::We isolated and characterized embryonic lethal mutations in piragua (prg). The prg locus encodes a protein with an amino terminus Zinc Finger-Associated-Domain (ZAD) and nine C2H2 zinc fingers (ZF). prg mRNA and protein expression during embryogenesis is dynamic with widespread maternal contribution, and subsequent ex...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2016.12.003
更新日期:2017-04-01 00:00:00
abstract::ZT3, isolated from a murine muscle cell cDNA library by a low-stringency hybridization, encodes a zinc finger domain containing factor with a transcript of 5.0 kb. A 3' 2.5 kb partial nucleotide sequence contains an ORF of 1.5 kb where 17 canonical C2H2 zinc finger domains organized in tandem were identified. It maps ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(95)00465-3
更新日期:1996-01-01 00:00:00
abstract::We have characterised a short (30 base pair) element from the Xenopus Wnt-5a promoter which is nearly identical to one located in the human Wnt-5a promoter, and has the same position relative to the transcription start site. When placed in front of a LacZ gene, this element can reproduce the same expression pattern ob...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00091-x
更新日期:1999-07-01 00:00:00
abstract::Unfertilized eggs of Oryzias latipes were artificially inseminated and incubated at 26+/-1 degrees C. Careful observation of the process of embryonic development by light microscopy allowed division of the process into 39 stages based on diagnostic features of the developing embryos. The principal diagnostic features ...
journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/j.mod.2004.03.012
更新日期:2004-07-01 00:00:00
abstract::In multicellular organisms, very little is known about the role of mRNA stability in development, and few proteins involved in degradation pathways have been characterized. We have identified the Drosophila homologue of XRN1, which is the major cytoplasmic 5'-3' exoribonuclease in Saccharomyces cerevisiae. The protein...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00173-7
更新日期:1998-12-01 00:00:00
abstract::Blood vessel maturation, which is characterized by the investment of vascular smooth muscle cells (vSMCs) around developing blood vessels, begins when vessels remodel into a hierarchy of proximal arteries and proximal veins that branch into smaller distal capillaries. The ultimate result of maturation is formation of ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2019.02.002
更新日期:2019-04-01 00:00:00
abstract::Hair cell (HC) and supporting cell (SC) productions are completed during early embryonic development of the mammalian cochlea. This study shows that acutely dissociated cells from the newborn rat organ of Corti, developed into so-called otospheres consisting of 98% nestin (+) cells when plated on a non-adherent substr...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00642-6
更新日期:2002-03-01 00:00:00
abstract::To investigate the origin and nature of the signals responsible for specification of the dermatomal lineage, excised axial organs in 2-day-old chick embryos were replaced by grafts of the dorsal neural tube, or the ventral neural tube plus the notochord, or aggregates of cells engineered to produce Sonic hedgehog (Shh...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00540-2
更新日期:2001-02-01 00:00:00
abstract::The sex determining gene, Sry, determines the sex of the organism by initiating development of a testis rather than an ovary from the cells of the bipotential gonad. In the 10 years since the discovery of Sry, new genes and cellular pathways that operate in the establishment of the gonadal primordium and the initiatio...
journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/s0925-4773(99)00327-5
更新日期:2000-03-15 00:00:00
abstract::T-box genes constitute a conserved multi-gene family with important roles in many developmental processes. In this report, we describe the cloning and expression analysis of a novel mouse T-box gene, Tbx20. Expression is prominent in the extraembryonic mesoderm, in the developing heart, the eye anlage and motor neuron...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00499-8
更新日期:2001-01-01 00:00:00
abstract::We describe the cloning, expression pattern and functional overexpression analysis of Xotx5b, a new member of the Otx gene family in Xenopus laevis. Early expression of Xotx5b resembles that of Xotx2, being detected in the organizer region at early gastrula stage, and, shortly after, also in anterior neuroectoderm. Du...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00367-1
更新日期:2000-08-01 00:00:00
abstract::Mastermind, which is a Notch signal component, is a nuclear protein and is thought to contribute to the transactivation of target genes. Previously we showed that XMam1, Xenopus Mastermind1, was essential in the transactivation of a Notch target gene, XESR-1, and was involved in primary neurogenesis. To examine the fu...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2006.08.001
更新日期:2006-11-01 00:00:00
abstract::The forkhead type Brain Factor 2 from mouse and chicken help pattern the forebrain, optic vesicle and kidney. We have isolated a Xenopus homolog (Xbf2) and found that during gastrulation it is expressed in the dorsolateral mesoderm, where it helps specify this territory by downregulating BMP-4 and its downstream genes...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00190-7
更新日期:1999-01-01 00:00:00
abstract::We have sequenced and defined the expression during rat embryogenesis of the protocadherin fat, the murine homologue of a Drosophila tumour suppressor gene. As previously described for human fat, the sequence encodes a large protocadherin with 34 cadherin repeats, five epidermal growth factor (EGF)-like repeats contai...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00217-2
更新日期:1999-02-01 00:00:00
abstract::There has been rapid progress recently in the identification of signalling pathways regulating tooth development. It has become apparent that signalling networks involved in Drosophila development and development of mammalian organs such as the limb are also used in tooth development. Teeth are epithelial appendages f...
journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/s0925-4773(97)00115-9
更新日期:1997-10-01 00:00:00
abstract::Sexual reproduction in the ciliate Tetrahymena follows a complex developmental program involving the sequential regulation of dozens of genes. Genes that are up-regulated during post-zygotic development in Tetrahymena were isolated by subtractive hybridization. Anlagen stage induced gene 1 (ASI1) encodes a 2.8 kb tran...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(02)00259-9
更新日期:2002-10-01 00:00:00
abstract::Each sensory organ of the Drosophila peripheral nervous system is derived from a single sensory organ precursor cell (SOP). These originate in territories defined by expression of the proneural genes of the Achaete-Scute complex (AS-C). Formation of ectopic sensilla outside these regions is prevented by transcriptiona...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(02)00183-1
更新日期:2002-09-01 00:00:00
abstract::Members of the TGFbeta superfamily of signalling molecules play important roles in mesendoderm induction and dorsoventral patterning of the vertebrate embryo. We cloned three intracellular mediators of TGFbeta signalling, smad1, 2 and 5, from the zebrafish. The three smad genes are expressed ubiquitously at the onset ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00173-2
更新日期:1999-10-01 00:00:00
abstract::Wnt-4 signaling plays a critical role in kidney development and is associated with the epithelial conversion of the metanephric mesenchyme. Furthermore, secreted Frizzled-related proteins (sFRPs) that can bind Wnts are normally expressed in the developing metanephros, and function in other systems as modulators of Wnt...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00282-9
更新日期:2001-04-01 00:00:00
abstract::A new family of homeodomain proteins has recently been identified that includes extradenticle, ceh-20 and three mammalian proteins Pbx1, Pbx2 and Pbx3. We show here that two members of this family, Pbx1 and Pbx2 bind cooperatively to DNA with both Hoxb-7 and Hoxb-8. Engrailed-2 modulates the DNA binding activity of th...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(95)00394-g
更新日期:1995-07-01 00:00:00