Abstract:
:BMP2, like its Drosophila homologue dpp, is an important signaling molecule for specification of cardiogenic mesoderm in vertebrates. Here, we analyzed the time-course of BMP2-requirement for early heart formation in whole chick embryos and in explants of antero-lateral plate mesoderm. Addition of Noggin to explants isolated at stage 4 and cultured for 24 h resulted in loss of NKX2.5, GATA4, eHAND, Mef2A and vMHC expression. At stages 5-8 the individual genes showed differential sensitivity to Noggin addition. While expression of eHAND, NKX2.5 and Mef2A was clearly reduced by Noggin vMHC was only marginally affected. In contrast, GATA4 expression was enhanced after Noggin treatment. The developmental period during which cardiac mesoderm required the presence of BMP signaling in vivo was assessed by implantation of Noggin expressing cells into stage 4-8 embryos which were then cultured until stage 10-11. Complete loss of NKX2.5 and eHAND expression was observed in embryos implanted at stages 4-6, and expression was still suppressed in stages 7 and 8 implanted embryos. GATA4 expression was also blocked by Noggin at stage 4, however increased at stages 5, 6 and 7. Explants of central mesendoderm, that normally do not form heart tissue were employed to study the time-course of BMP2-induced cardiac gene expression. The induction of cardiac lineage markers in central mesendoderm of stage 5 embryos was distinct for different genes. While GATA4, -5, -6 and MEF2A were induced to maximal levels within 6 h after BMP2 addition, eHAND and dHAND required 12 h to reach maximum levels of expression. NKX2.5 was induced by 6 h and accumulated over 48 h. vMHC and titin were induced at significant levels only after 48 h of BMP2 addition. These results indicate that cardiac marker genes display distinct expression kinetics after BMP2 addition and differential response to Noggin treatment suggesting complex regulation of myocardial gene expression in the early tubular heart.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Schlange T,Andrée B,Arnold HH,Brand Tdoi
10.1016/s0925-4773(99)00311-1subject
Has Abstractpub_date
2000-03-01 00:00:00pages
259-70issue
1-2eissn
0925-4773issn
1872-6356pii
S0925-4773(99)00311-1journal_volume
91pub_type
杂志文章abstract::Syndecans are a family of heparan sulfate proteoglycans implicated in cell-cell and cell-matrix interactions. To investigate the roles of syndecans in early development, we identified three syndecan family members in Xenopus laevis: Xsyn-1, Xsyn-2, and Xsyn-3. Xsyn-1 and Xsyn-2 are maternal mRNAs localized to the anim...
journal_title:Mechanisms of development
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abstract::Compelling evidence from in vivo model systems within the past decade shows that the APP family of proteins is important for synaptic development and function in the central and peripheral nervous systems. The synaptic role promises to be complex and multifaceted for several reasons. The three family members have over...
journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00058-6
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journal_title:Mechanisms of development
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更新日期:1999-12-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2012-07-01 00:00:00
abstract::The maturation of T cells is an intricate process involving the interaction of developing thymocytes with discrete microenvironments within the thymus. Numerous studies have indicated that distinct thymic compartments provide signals required for each stage of thymocyte maturation. In this study we performed a compreh...
journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2008.01.008
更新日期:2008-05-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00006-9
更新日期:1998-02-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:1997-07-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00115-4
更新日期:1998-10-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2010.03.003
更新日期:2010-05-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2019.04.002
更新日期:2019-06-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2001-05-01 00:00:00
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