Abstract：
:The homeotic or Hox genes encode a network of conserved transcription factors which provide axial positional information and control segment morphology in development and evolution. During embryonic brain development of Drosophila, the Hox gene labial (lab) is essential for tritocerebral neuromere specification; lab loss of function results in tritocerebral cells that fail to adopt a neuronal identity, causing axonal pathfinding defects. Here we present evidence that the POU-homeodomain DNA-binding protein ventral veins lacking (vvl) acts genetically downstream of lab in the specification of the tritocerebral neuromere. In the embryonic brain, vvl expression is seen in all brain neuromeres, including the tritocerebral lab domain. Lab mutant analysis shows that vvl expression in the tritocerebrum is dependent on lab activity. Loss-of-function analysis focussed on the tritocerebrum reveals that inactivation of vvl results in patterning defects which are comparable to the brain phenotype caused by null mutation of lab. In the absence of vvl, mutant tritocerebral cells are generated and positioned correctly, but these cells fail to express neuronal markers indicating defects in neuronal differentiation. Moreover, longitudinal axon pathways in the tritocerebrum are severely reduced or absent and the tritocerebral commissure is missing in the vvl mutant brain. Genetic rescue experiments show that vvl is able to partially replace lab in the specification of the tritocerebral neuromere. Our results indicate that vvl acts downstream of the Hox gene lab and regulates specific aspects of neuronal differentiation within the tritocerebral neuromere during embryonic brain development of Drosophila.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Meier S,Sprecher SG,Reichert H,Hirth Fdoi
10.1016/j.mod.2005.09.004subject
Has Abstractpub_date
2006-01-01 00:00:00pages
76-83issue
1eissn
0925-4773issn
1872-6356pii
S0925-4773(05)00161-9journal_volume
123pub_type
杂志文章abstract：:The neural crest (NC) is one of the most fascinating structures during embryonic development. Unique to vertebrate embryos, these cells give rise to important components of the craniofacial skeleton, such as the jaws and skull, as well as melanocytes and ganglia of the peripheral nervous system. Worldwide, several gro...
journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/j.mod.2018.07.009
更新日期：2018-10-01 00:00:00
abstract：:Active repression of gene expression mediated by unliganded nuclear receptors plays crucial roles in early development of vertebrates. N-CoR (nuclear receptor co-repressor) is the first identified co-repressor that can repress retinoic acid (RA) inducible gene transcription in the absence of RA. Previously, N-CoR was ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2009.09.001
更新日期：2009-10-01 00:00:00
abstract：:The spatial and temporal analysis of GATA-3 expression pattern in the human embryo revealed its expression in new anatomical sites. These include the endoderm of the primitive foregut, pharynx and allantois, the branchial arches and the mesenchymal cells surrounding the stomach and dorsal aorta. On the other hand, hum...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00088-x
更新日期：1999-07-01 00:00:00
abstract：:Five chick homeodomain proteins (CHOXs), CHOX-1.7, -1.1, -1.4, -4.2 and -2.6, had different transcription-regulating activities in a chick cultured cell line, LMH. In particular, CHOX-1.7 highly activated transcription when NP6 was used as the target site whereas CHOX-1.4 did not. This was mainly due to differences in...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(92)90012-9
更新日期：1992-03-01 00:00:00
abstract：:Myogenin and CMD1, the chicken homologue of MyoD, transactivate the promoter of the alpha-subunit of the acetylcholine receptor (AChR) in chicken fibroblasts. The expression of these three genes was followed by in situ hybridization. In two-day-old embryos the CMD1 gene is expressed shortly before the AChR alpha-subun...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(92)90018-f
更新日期：1992-03-01 00:00:00
abstract：:We have identified a novel chicken gene, cMespo, which encodes a basic-helix-loop-helix (bHLH) protein with sequence homology to a subgroup of bHLH transcription factors that have been implicated in somitogenesis. cMespo transcripts are first found in the primitive streak of gastrulating chick embryos (HH stage 4) and...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00424-x
更新日期：2000-10-01 00:00:00
abstract：:Using an in silico (electronic database) subtraction, we identified a new member of the Ret Finger Protein-Like gene family, Rfpl4. Rfpl4 encodes a 287 amino acid putative E3 ubiquitin-protein ligase with a RING finger-like domain and a B30.2 motif. Reverse transcriptase polymerase chain reaction and Northern blot ana...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00635-9
更新日期：2002-03-01 00:00:00
abstract：:Formation of tubular structures from an epithelial tissue is a process common to many morphogenetic events during organogenesis. We report here new data concerning the expression pattern of the vHNF1/HNF1beta gene during this process in the mouse. vHNF1 (variant Hepatocyte Nuclear Factor 1) is a member of the HNF1 hom...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00221-x
更新日期：1999-12-01 00:00:00
abstract：:The transcription factor scleraxis (SCX) is expressed throughout tendon development and plays a key role in directing tendon wound healing. However, little is known regarding its role in fetal or young postnatal tendons, stages in development that are known for their enhanced regenerative capabilities. Here we used RN...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2020.103635
更新日期：2020-09-01 00:00:00
abstract：:A gene transfer method to ectopically express genes during chicken limb pattern formation using replication defective retroviral vectors has been established. Spherical non-proliferating (mitomycin C treated) aggregates of clonal retrovirus producing cells were grafted directly into developing chicken wing buds. The c...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(93)90035-v
更新日期：1993-10-01 00:00:00
abstract：:This study compares by immunogold labeling the ultrastructural localization of a hexameric 22S glycoprotein, called toposome, with that of hyalin in unfertilized eggs and cells of hatched sea urchin blastulae. Nearly all hyalin is present in the electron translucent compartment of the cortical granules and in the tran...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(91)90079-l
更新日期：1991-02-01 00:00:00
abstract：:Increases in Na/K-ATPase activity occur concurrently with the onset of cavitation and are associated with increases in Na(+)-pump subunit mRNA and protein expression. We have hypothesized that the alpha1-isozyme of the Na/K-ATPase is required to mediate blastocyst formation. We have tested this hypothesis by character...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2004.04.005
更新日期：2004-05-01 00:00:00
abstract：:In bovine preimplantation development, female embryos progress at lower rates and originate smaller blastocysts than male counterparts. Although sex-specific gene expression patterns are reported, when and how sex dimorphism is established is not clear. Differences among female and male early development can be useful...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2015.12.001
更新日期：2016-02-01 00:00:00
abstract：:In mammals, the SWI/SNF complex is involved in chromatin remodelling in a wide range of cellular events for which regulatory factors require access to DNA. In the present study, we analyzed in early postimplantation mouse embryos the expression pattern of BRM (SNF2alpha) and BRG1 (SNF2beta), which are both ATPase subu...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00560-8
更新日期：2001-03-01 00:00:00
abstract：:Craniofacial development is a highly conserved process that requires complex interactions between neural crest cells (NCCs) and pharyngeal tissues derived from all three germ layers. Signals emanating from the pharyngeal endoderm drive differentiation of NCCs into craniofacial cartilage, and disruption of this process...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2014.05.005
更新日期：2014-08-01 00:00:00
abstract：:The localization of RNAs at the vegetal cortex in Xenopus oocytes is a complex process, involving at least two different pathways. The early, or messenger transport organizer (METRO), pathway, localizes RNAs such as Xlsirts, Xcat2 and Xwnt11 during stages 1 and 2 of oogenesis, while the late pathway localizes RNAs suc...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00041-0
更新日期：1998-04-01 00:00:00
abstract：:The scaffolding protein tetraspanin18 (Tspan18) maintains epithelial cadherin-6B (Cad6B) to antagonize chick cranial neural crest epithelial-to-mesenchymal transition (EMT). For migration to take place, Tspan18 must be downregulated. Here, we characterize the role of the winged-helix transcription factor FoxD3 in the ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2014.02.004
更新日期：2014-05-01 00:00:00
abstract：:Skeletal muscle regeneration requires processes different from developmental myogenesis. One important difference is a requirement of inflammatory reactions prior to regenerative myogenesis, by which injured muscle fibers must be eliminated to make new myotubes. In this study, we show that efficient elimination of inj...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2014.12.001
更新日期：2015-02-01 00:00:00
abstract：:The effects of signal perturbation on expression domains of molecular markers for the mesoderm and ectoderm have been analysed across the dorso-ventral axis in zebrafish embryos. Injection of RNA encoding bone morphogenetic protein-4 (BMP-4) ventralised the embryo, expanding the intermediate mesoderm and non-neural ec...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00659-x
更新日期：1997-03-01 00:00:00
abstract：:Using in situ hybridization on whole-mounts and sections of mouse embryos we have visualized the pattern of expression for the Eph receptor ligand ephrin-A5. Non neuronal expression domains include the ectoderm of the branchial arches, the ectoderm and mesenchyme surrounding the dorsal root ganglia, the intervertebral...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00252-5
更新日期：2000-05-01 00:00:00
abstract：:Mll, Brg1 and Brm are vertebrate homologues of Drosophila trithorax group (trxG) genes. We isolated chicken Mll cDNA clones, and examined patterns of Mll, Brg1 and Brm expression in chick embryos. All three genes were expressed from embryonic stage 2 onwards. Mll transcripts were just detectable in all tissues by in s...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00207-x
更新日期：1999-01-01 00:00:00
abstract：:In the developing eye of Drosophila the protein kinase D-Raf controls the specification of the R7 photoreceptor cells. We show that overexpression of wild-type D-Raf inhibits the formation of R7 cells in a dose-dependent manner. Conversely, overexpression of mutant D-Raf proteins in which the conserved S388 is replace...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00052-x
更新日期：1997-06-01 00:00:00
abstract：:Homeobox genes regulate multiple aspects of mouse development including the axial patterning in the central nervous system. Some of the more divergent members of this gene family are expressed in the anterior region of the central nervous system where the Hox genes are not expressed. We previously reported the isolati...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(94)90089-2
更新日期：1994-08-01 00:00:00
abstract：:We have generated a null allele of the mouse Msx1 homeobox gene by insertion of an nlacZ reporter gene into its homeobox. The sensitivity of beta-galactosidase detection permitted us to reveal novel aspects of Msx1 gene expression in heterozygous embryos, in particular in ectoderm and mesoderm during gastrulation, and...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00065-8
更新日期：1997-07-01 00:00:00
abstract：:Directional migration of primordial germ cells (PGCs) toward future gonads is a common feature in many animals. In zebrafish, mouse and chicken, SDF-1/CXCR4 chemokine signaling has been shown to have an important role in PGC migration. In Xenopus, SDF-1 is expressed in several regions in embryos including dorsal mesod...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2009.09.005
更新日期：2010-01-01 00:00:00
abstract：:The enteric nervous system (ENS) derives from migratory neural crest cells that colonize the developing gut tube, giving rise to an integrated network of neurons and glial cells, which together regulate important aspects of gut function, including coordinating the smooth muscle contractions of the gut wall. The absenc...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2008.04.006
更新日期：2008-08-01 00:00:00
abstract：:Retinoic acid plays important roles in development, growth and differentiation by regulating the expression of target genes. A new retinoic acid-inducible gene, Stra6, has been identified in P19 embryonal carcinoma cells using a subtractive hybridization cDNA cloning technique. Stra6 codes for a very hydrophobic membr...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00039-7
更新日期：1997-05-01 00:00:00
abstract：:We have identified a Drosophila gene encoding a putative receptor tyrosine kinase by screening a genomic DNA library with a DNA probe for a Drosophila homolog of fibroblast growth factor receptors. The newly isolated gene codes for a transmembrane protein most similar in sequence to a mammalian proto-oncogene ret; thu...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(94)90027-2
更新日期：1994-02-01 00:00:00
abstract：:The POU domain is a approximately 140 amino acid domain shared by several mammalian transcriptional activators. We have isolated genomic and cDNA clones from three Drosophila POU domain genes, designated pdm-1, pdm-2, and Cf1a. All three genes encode a conserved POU-specific domain and a POU homeo domain but are other...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(91)90045-8
更新日期：1991-06-01 00:00:00
abstract：:The Ras protein activates at least three different pathways during early development. Two of them regulate mesodermal gene expression and the third is thought to participate in the control of actin cytoskeleton dynamics via the Ral protein. From a yeast two-hybrid screen of a Xenopus maternal cDNA library, we identifi...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2004.07.008
更新日期：2004-12-01 00:00:00
