Abstract：
:Using an in silico (electronic database) subtraction, we identified a new member of the Ret Finger Protein-Like gene family, Rfpl4. Rfpl4 encodes a 287 amino acid putative E3 ubiquitin-protein ligase with a RING finger-like domain and a B30.2 motif. Reverse transcriptase polymerase chain reaction and Northern blot analyses reveal that Rfpl4 encodes a 1.7kb mRNA detectable exclusively in the gonads of adult mice. In situ hybridization localizes Rfpl4 transcripts within the ovary to oocytes of primary and later stage follicles and in the testis to elongating spermatids. The Rfpl4 gene comprises three exons and maps to mouse chromosome 7. We have identified the human ortholog, which maps to 19q13.4. These studies suggest that RFPL4 mediates protein degradation pathways important for gametogenesis or early embryonic development.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Rajkovic A,Lee JH,Yan C,Matzuk MMdoi
10.1016/s0925-4773(01)00635-9subject
Has Abstractpub_date
2002-03-01 00:00:00pages
173-7issue
1-2eissn
0925-4773issn
1872-6356pii
S0925477301006359journal_volume
112pub_type
杂志文章abstract：:The cloning of a Pax6 orthologue from the sepiolid squid Euprymna scolopes and its developmental expression pattern are described. The data are consistent with the presence of a single gene encoding a protein with highly conserved DNA-binding paired and homeodomains. A detailed expression analysis by in situ hybridiza...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(02)00456-2
更新日期：2003-02-01 00:00:00
abstract：:In situ hybridization of mouse embryo whole mounts and sagittal sections revealed a tissue- and stage-specific expression pattern of the transcriptionally regulated serum and glucocorticoid-inducible protein kinase (sgk) during embryogenesis. Sgk expression is first observed at embryonic day 8.5 (E8.5) in the decidua ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00351-3
更新日期：2001-05-01 00:00:00
abstract：:Noelins are secreted glycoproteins with important developmental functions in frogs and birds. Here, we present the expression pattern of the mouse homolog of Noelin-1/2 at E8-10 of development and compare this pattern to other vertebrates. Expression was observed in the neural plate and neural crest, as well as in the...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(02)00308-8
更新日期：2002-11-01 00:00:00
abstract：:In this paper we analyze the expression in follicular cells and regulation of the vitelline membrane protein gene we identified in region 32E of the second chromosome of D. melanogaster (VMP32E). We report germ line transformation results obtained with different kinds of gene fusion leading to the identification of a ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(91)90018-2
更新日期：1991-11-01 00:00:00
abstract：:Recent work identified an apoptotic program in gastrulation stage Xenopus embryos (Anderson, J.A., Lewellyn, A.L., Maller, J.L., 1997. Mol. Biol. Cell 8, 1195-1206; Stack, J.H., Newport, J.W., 1997. Development 124, 3185-3195). Here, we characterize in detail this maternal cell death program, which is set up at fertil...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00191-3
更新日期：1997-12-01 00:00:00
abstract：:A key role for phosphorylation of Smad2 by TGFβ superfamily ligands in the axial patterning of early embryos is well established. The regulation and role of Smad2 signaling in post-neurula embryonic patterning, however, is less well understood. While a variety of TGFβ superfamily ligands are implicated in various stag...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2010.08.004
更新日期：2010-09-01 00:00:00
abstract：:MicroRNAs (miRNAs) play important roles in regulating gene expression during numerous biological/pathological processes. Dicer encodes an RNase III endonuclease that is essential for generating most, if not all, functional miRNAs. In this work, we applied a conditional gene inactivation approach to examine the functio...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2010.12.002
更新日期：2011-03-01 00:00:00
abstract：:Polycomb group (PcG) proteins regulate and maintain expression pattern of genes set early during development. Although originally isolated as regulators of homeotic genes, PcG members play a key role in epigenetic mechanisms that maintain the expression state of a large number of genes. All members of the two polycomb...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2015.07.013
更新日期：2015-11-01 00:00:00
abstract：:Blood vessel maturation, which is characterized by the investment of vascular smooth muscle cells (vSMCs) around developing blood vessels, begins when vessels remodel into a hierarchy of proximal arteries and proximal veins that branch into smaller distal capillaries. The ultimate result of maturation is formation of ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2019.02.002
更新日期：2019-04-01 00:00:00
abstract：:Mammalian and Drosophila homologues of Baf57 have been previously isolated as being a subunit of SWI/SNF-like chromatin remodeling complexes. Here, we report the cloning and developmental expression of Xenopus Baf57. We isolated XBaf57 by using an expression cloning approach to identify novel modulators of Xenopus Sma...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(02)00129-6
更新日期：2002-08-01 00:00:00
abstract：:Basic helix-loop-helix (bHLH) proteins of the Achaete/Scute (Ac/Sc) family are required for neurogenesis in both Drosophila and vertebrates. These transcription factors are commonly referred to as 'proneural' factors, as they promote neural fate in many contexts. Although Ac/Sc proteins have been studied in Hydra, jel...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2008.06.002
更新日期：2008-09-01 00:00:00
abstract：:Deflecting biomineralized crystals attached to vestibular hair cells are necessary for maintaining balance. Zebrafish (Danio rerio) are useful organisms to study these biomineralized crystals called otoliths, as many required genes are homologous to human otoconial development. We sought to identify and characterize t...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2019.04.001
更新日期：2019-06-01 00:00:00
abstract：:Lipids, in addition to being structural components of cell membranes, can act as signaling molecules. Bioactive lipids, such as sphingosine 1-phosphate (S1P) and lysophosphatidic acid (LPA), may act intracellularly as second messengers or be secreted and act as intercellular signaling molecules. Such molecules can aff...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(03)00131-x
更新日期：2002-12-01 00:00:00
abstract：:By means of subtractive and differential hybridization techniques we have identified a novel murine gene (1A13) the expression of which is developmentally regulated in the mouse brain. Comparison of the nucleotide and predicted protein sequence revealed closest relationship of 1A13 to human CoREST, a transcriptional c...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00477-4
更新日期：2001-10-01 00:00:00
abstract：:During the development of the proventriculus (glandular stomach) of the chicken embryo, the endodermal epithelium invades into the surrounding mesenchyme and forms glands. The glandular epithelial cells produce pepsinogen, while the non-glandular (luminal) epithelial cells secrete mucus. Sonic hedgehog is expressed un...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(03)00069-8
更新日期：2003-07-01 00:00:00
abstract：:The homeotic or Hox genes encode a network of conserved transcription factors which provide axial positional information and control segment morphology in development and evolution. During embryonic brain development of Drosophila, the Hox gene labial (lab) is essential for tritocerebral neuromere specification; lab l...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2005.09.004
更新日期：2006-01-01 00:00:00
abstract：:Mouse embryos lacking Gsc and Dkk1 function display severe deficiencies in craniofacial structures which are not found in either Dkk1 homozygous null or Gsc homozygous null mutant embryos. Loss of Gsc has a dosage-related effect on the severity of head truncation phenotype in Dkk1 heterozygous embryos. The synergistic...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2006.10.001
更新日期：2007-02-01 00:00:00
abstract：:Cloning and sequencing of mouse Mf2 (mesoderm/mesenchyme forkhead 2) cDNAs revealed an open reading frame encoding a putative protein of 492 amino acids which, after in vitro translation, binds to a DNA consensus sequence. Mf2 is expressed at high levels in the ventral region of newly formed somites, in sclerotomal de...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00157-3
更新日期：1998-01-01 00:00:00
abstract：:In a screen for receptor tyrosine kinase genes regionally expressed in the developing hindbrain, we cloned and characterised the chicken ret gene. Data derived from studies of congenital human disease and from disruption of murine ret have demonstrated roles for ret in development of the kidney and enteric nervous sys...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(95)00449-1
更新日期：1995-11-01 00:00:00
abstract：:Dickkopf-1 (dkk-1) is member of a novel family of secreted proteins and functions in head induction during Xenopus embryogenesis, acting as a potent inhibitor of Wnt signalling. Here we report: (1) the isolation of two additional murine members of the dkk family, dkk-2 and dkk-3; and (2) analysis of adult and embryoni...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00138-0
更新日期：1999-09-01 00:00:00
abstract：:Germ-line mutations of LKB1 and PTEN tumor suppressor genes underlie the phenotypically related Peutz-Jeghers syndrome (PJS) and Cowden disease (CD), respectively. To analyze possible developmental roles of PTEN and LKB1, we have studied their mRNA expression during mouse embryonic development (E7-17.5) by in situ hyb...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00050-7
更新日期：1999-05-01 00:00:00
abstract：:The tramtrack (ttk) gene of Drosophila encodes 69-kDa and 88-kDa proteins through alternative splicing of the primary ttk transcript. The two proteins share a common amino-terminal sequence, but contain different carboxy-terminal portions, each of which has a distinct zinc finger domain with a unique DNA binding speci...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(92)90052-l
更新日期：1992-09-01 00:00:00
abstract：:We have cloned a chick homologue of Drosophila dachshund (dac), termed Dach1. Dach1 is the orthologue of mouse and human Dac/Dach (hereafter referred to as Dach1). We show that chick Dach1 is expressed in a variety of sites during embryonic development, including the eye and ear. Previous work has demonstrated the exi...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00611-6
更新日期：2002-02-01 00:00:00
abstract：:We have characterised a short (30 base pair) element from the Xenopus Wnt-5a promoter which is nearly identical to one located in the human Wnt-5a promoter, and has the same position relative to the transcription start site. When placed in front of a LacZ gene, this element can reproduce the same expression pattern ob...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00091-x
更新日期：1999-07-01 00:00:00
abstract：:To examine the role of the Oct-6 gene in Schwann cell differentiation we have cloned and characterized the chicken and zebrafish homologues of the mouse Oct-6 gene. While highly homologous in the Pit1-Oct1/2-Unc86 (POU) domain, sequence similarities are limited outside this domain. Both genes are intronless and both p...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00067-7
更新日期：1998-06-01 00:00:00
abstract：:Early in the development of the imaginal wing disc of Drosophila, the LIM-HD gene tailup (islet), together with the HD genes of the iroquois complex, specify the notum territory of the disc. Later, tailup has been shown to act as a prepattern gene that antagonizes formation of sensory bristles on the notum of this fly...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2010.05.001
更新日期：2010-09-01 00:00:00
abstract：:Homeobox genes regulate multiple aspects of mouse development including the axial patterning in the central nervous system. Some of the more divergent members of this gene family are expressed in the anterior region of the central nervous system where the Hox genes are not expressed. We previously reported the isolati...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(94)90089-2
更新日期：1994-08-01 00:00:00
abstract：:Medaka is an ideal model system for developmental studies as it combines the advantages of powerful genetics and classical embryology. Due to the accessibility, transparency and fast development, embryogenesis and morphogenesis can be followed in vivo. Microscopic time-lapse imaging, however, requires the immobilizati...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2004.03.016
更新日期：2004-07-01 00:00:00
abstract：:We studied the expression of MUL, a gene encoding a novel member of the RING-B-Box-Coiled Coil family of zinc finger proteins that underlies the human inherited disorder, Mulibrey nanism. In early human and mouse embryogenesis MUL is expressed in dorsal root and trigeminal ganglia, liver and in epithelia of multiple t...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00491-9
更新日期：2001-10-01 00:00:00
abstract：:Cadherins are Ca(+2)-dependent cell surface proteins involved in the specification of the adhesive properties of cells. They are supposed to play a critical role in morphogenesis and pattern formation. In this paper we show that in the sea urchin embryo there are at least two different cadherins of relative molecular ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(93)90054-2
更新日期：1993-04-01 00:00:00
