Abstract:
:In bovine preimplantation development, female embryos progress at lower rates and originate smaller blastocysts than male counterparts. Although sex-specific gene expression patterns are reported, when and how sex dimorphism is established is not clear. Differences among female and male early development can be useful for human assisted reproductive medicine, when X-linked disorders risk is detected, and for genetic breeding programs, especially in dairy cattle, which requires female animals for milk production. The aim of this study was to characterize the development of female and male embryos, attempting to identify sex effects during preimplantation development and the role of cell death in this process. Using sex-sorted semen from three different bulls for fertilization, we compared kinetics of bovine sex-specific embryos in six time points, and cell death was assessed in viable embryos. For kinetics analysis, we detected an increased population of female embryos arrested at 48 and 120h.p.i., suggesting this time points as delicate stages of development for female embryos that should be considered for testing improvement strategies for assisted reproductive technologies. Assessing viable embryos quality, we found 144h.p.i. is the first time point when viable embryos are phenotypically distinct: cell number is decreased, and apoptosis and cell fragmentation are increased in female embryos at this stage. These new results lead us to propose that sex dimorphism in viable embryos is established during morula-blastocyst transition, and cell death is involved in this process.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Oliveira CS,Saraiva NZ,de Lima MR,Oliveira LZ,Serapião RV,Garcia JM,Borges CA,Camargo LSdoi
10.1016/j.mod.2015.12.001subject
Has Abstractpub_date
2016-02-01 00:00:00pages
42-50eissn
0925-4773issn
1872-6356pii
S0925-4773(15)30038-1journal_volume
139pub_type
杂志文章abstract::Deflecting biomineralized crystals attached to vestibular hair cells are necessary for maintaining balance. Zebrafish (Danio rerio) are useful organisms to study these biomineralized crystals called otoliths, as many required genes are homologous to human otoconial development. We sought to identify and characterize t...
journal_title:Mechanisms of development
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更新日期:2019-06-01 00:00:00
abstract::The Ras protein activates at least three different pathways during early development. Two of them regulate mesodermal gene expression and the third is thought to participate in the control of actin cytoskeleton dynamics via the Ral protein. From a yeast two-hybrid screen of a Xenopus maternal cDNA library, we identifi...
journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/s0925-4773(97)00115-9
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journal_title:Mechanisms of development
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abstract::Vertebrates display striking left-right asymmetries in the placement of internal organs, which are concealed by a seemingly bilaterally symmetric body plan. The establishment of asymmetries about the left-right axis occurs early during embryo development and requires the concerted and sequential action of several epig...
journal_title:Mechanisms of development
pub_type: 杂志文章,评审
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abstract::Egr1 is a highly conserved zinc finger protein which plays important roles in many aspects of vertebrate development and in the adult. The cDNA coding for zebrafish Egr1 was obtained and its expression pattern was examined during zebrafish embryogenesis using whole-mount in situ hybridization. Egr1 mRNA is first detec...
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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更新日期:1996-02-01 00:00:00
abstract::Drosophila Spitz is a homolog of transforming growth factor alpha (TGF-alpha) and is an activating ligand for the EGF receptor (Egfr). It has been shown that Star is required for Spitz activity. Here we show that Star is quantitatively limiting for Spitz production during eye development. We also show that Star and Sp...
journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2003.09.001
更新日期:2003-10-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2008.10.005
更新日期:2009-01-01 00:00:00
abstract::Five chick homeodomain proteins (CHOXs), CHOX-1.7, -1.1, -1.4, -4.2 and -2.6, had different transcription-regulating activities in a chick cultured cell line, LMH. In particular, CHOX-1.7 highly activated transcription when NP6 was used as the target site whereas CHOX-1.4 did not. This was mainly due to differences in...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(92)90012-9
更新日期:1992-03-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/j.mod.2012.11.001
更新日期:2013-06-01 00:00:00
abstract::We have identified a Drosophila gene encoding a putative receptor tyrosine kinase by screening a genomic DNA library with a DNA probe for a Drosophila homolog of fibroblast growth factor receptors. The newly isolated gene codes for a transmembrane protein most similar in sequence to a mammalian proto-oncogene ret; thu...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(94)90027-2
更新日期:1994-02-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00115-4
更新日期:1998-10-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/j.mod.2004.04.022
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更新日期:2000-06-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(94)00303-5
更新日期:1995-01-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00207-x
更新日期:1999-01-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(96)00524-2
更新日期:1996-06-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00221-x
更新日期:1999-12-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(03)00121-7
更新日期:2002-12-01 00:00:00
abstract::Members of the cysteine-rich protein (CRP) define a subclass of LIM-only proteins implicated mainly in muscle differentiation. Until now, very little is known concerning the expression of CRP encoding genes during vertebrate development. We describe here the isolation of a trout (Oncorhynchus mykiss) gene encoding a c...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00127-0
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abstract::The effects of signal perturbation on expression domains of molecular markers for the mesoderm and ectoderm have been analysed across the dorso-ventral axis in zebrafish embryos. Injection of RNA encoding bone morphogenetic protein-4 (BMP-4) ventralised the embryo, expanding the intermediate mesoderm and non-neural ec...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(97)00659-x
更新日期:1997-03-01 00:00:00
abstract::A key role for phosphorylation of Smad2 by TGFβ superfamily ligands in the axial patterning of early embryos is well established. The regulation and role of Smad2 signaling in post-neurula embryonic patterning, however, is less well understood. While a variety of TGFβ superfamily ligands are implicated in various stag...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2010.08.004
更新日期:2010-09-01 00:00:00
abstract::Following tissue damage the immune response, including inflammation, has been considered an inevitable condition to build the host defense against invading pathogens. The recruitment of innate immune leukocytes to injured tissue is observed in both vertebrates and invertebrates. However, it is still not conclusive whe...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2012.11.005
更新日期:2013-02-01 00:00:00