Abstract：
:To understand the mechanisms regulating the tissue non-specific alkaline phosphatase (TNAP) activity during development, we characterized cis-transcriptional regulatory elements. In embryonic cells and tissues, TNAP expression was driven preferentially by the exon 1A (E1A) promoter, one of the two promoters previously defined. Transcriptional activity of E1A promoter was up-regulated by retinoic acid (RA) through a putative RA-responsive element. Transgenic mice analysis with lacZ reporter constructs revealed negative regulatory elements within 8.5 kb of E1A promoter. Promoter sequences of endogenous TNAP in non-expressing tissues and those carried by the 8.5 kb-lacZ transgene were found to be highly methylated. A 1 kb fragment of E1A promoter increased the methylation level of lacZ and promoter sequences. The role of RA and DNA methylation in defining the embryonic expression pattern of TNAP is discussed.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Escalante-Alcalde D,Recillas-Targa F,Hernández-García D,Castro-Obregón S,Terao M,Garattini E,Covarrubias Ldoi
10.1016/0925-4773(96)00524-2subject
Has Abstractpub_date
1996-06-01 00:00:00pages
21-32issue
1eissn
0925-4773issn
1872-6356pii
0925477396005242journal_volume
57pub_type
杂志文章abstract：:The vascularization of the central nervous system occurs by angiogenic sprouting, a process in which different factors like vascular endothelial growth factor (VEGF) and angiopoietin (Ang)-1/2 must act in a coordinated fashion. We investigated how these factors participate in capillarization of the cerebellum, an area...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00471-3
更新日期：2001-10-01 00:00:00
abstract：:In the process of cloning murine proteins capable of binding to a regulatory module of the Ncam promoter, we isolated a novel homeobox gene, Barx1, the first vertebrate member of the structural subclass defined by Drosophila BarH1. Here we report its sequence, chromosomal localisation and embryonic expression pattern....
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(94)00343-l
更新日期：1995-05-01 00:00:00
abstract：:XLPOU3 is a member of the Xenopus POU domain family of genes, encoding a class of homeodomain-containing transcription factors implicated in cell differentiation during development. Here we describe the isolation and characterisation of XLPOU3b, an allelic genomic counterpart of the XLPOU3 cDNA previously described (B...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(96)00563-1
更新日期：1996-08-01 00:00:00
abstract：:During the final stages of embryogenesis, the Drosophila embryo exhibits a dorsal hole covered by a simple epithelium of large cells termed the amnioserosa (AS). Dorsal closure is the process whereby this hole is closed through the coordination of cellular activities within both the AS and the epidermis. Genetic analy...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2007.09.002
更新日期：2007-11-01 00:00:00
abstract：:The forkhead type Brain Factor 2 from mouse and chicken help pattern the forebrain, optic vesicle and kidney. We have isolated a Xenopus homolog (Xbf2) and found that during gastrulation it is expressed in the dorsolateral mesoderm, where it helps specify this territory by downregulating BMP-4 and its downstream genes...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00190-7
更新日期：1999-01-01 00:00:00
abstract：:To examine the role of the Oct-6 gene in Schwann cell differentiation we have cloned and characterized the chicken and zebrafish homologues of the mouse Oct-6 gene. While highly homologous in the Pit1-Oct1/2-Unc86 (POU) domain, sequence similarities are limited outside this domain. Both genes are intronless and both p...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00067-7
更新日期：1998-06-01 00:00:00
abstract：:We describe the identification and detailed expression pattern of a second Drosophila Sox gene, SoxNeuro (SoxN), highly related to mammalian group B Sox1, 2, 3 genes. SoxN is expressed in a highly dynamic pattern during embyogenesis, being associated with the development of the central nervous system (CNS), from the e...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00268-9
更新日期：2000-05-01 00:00:00
abstract：:In Drosophila melanogaster, the patterning of dorsal appendages on the eggshell is strictly controlled by EGFR signaling. However, the number of dorsal appendages is remarkably diverse among Drosophila species. For example, D. melanogaster and D. virilis have two and four dorsal appendages, respectively. Here we show ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(03)00164-3
更新日期：2003-08-01 00:00:00
abstract：:Six genes are vertebrate homologues of the homeobox-containing gene sine oculis, which plays an essential role in controlling Drosophila compound eye development. Here we report the identification and expression patterns of all three subfamilies of Xenopus Six genes. Two Six2 subfamily genes (Six1, Six2) showed very s...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00572-4
更新日期：2001-03-01 00:00:00
abstract：:We investigated the expression pattern of the endothelin-A receptor and endothelin 1 genes, the mutations of which affect the development of the mesectodermal derivatives of the neural crest. We show here that endothelin 1 is expressed by the environment of the cephalic neural crest cells invading branchial arches. La...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00079-3
更新日期：1998-07-01 00:00:00
abstract：:Two cDNAs encoding Myb-related proteins have been cloned from Xenopus laevis and they have been termed Xmyb1 and Xmyb2. The Xmyb1 cDNA clone codes for an open reading frame of 733 amino acids and exhibits a high degree of similarity over the entire predicted protein sequence with the human B-Myb protein. Xmyb2 is a pa...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(92)90015-c
更新日期：1992-03-01 00:00:00
abstract：:All body pigment cells in vertebrates are derived from the neural crest. In fish the neural crest can generate up to six different types of pigment cells, as well as various non-pigmented derivatives. In mouse and zebrafish, extensive collections of pigmentation mutants have enabled dissection of many aspects of pigme...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2004.01.004
更新日期：2004-07-01 00:00:00
abstract：:Although the molecular mechanisms directing anteroposterior patterning of the Drosophila embryo (long-germband mode) are well understood, how these mechanisms were evolved from an ancestral mode of insect embryogenesis remains largely unknown. In order to gain insight into mechanisms of evolution in insect embryogenes...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2004.10.001
更新日期：2005-02-01 00:00:00
abstract：:In mammals, the Ror-family receptor tyrosine kinases consist of two structurally related proteins, Ror1 and Ror2, characterized by the extracellular Frizzled-like cysteine-rich domain and membrane proximal kringle domains. As an attempt to gain insights into their roles in mouse development, expression patterns of Ror...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00383-5
更新日期：2001-07-01 00:00:00
abstract：:The expression pattern of K(+) currents is the principal regulator of electrical activity during development of the nervous and muscular system. We report here a study showing the expression pattern of HERG K(+) currents-encoding (erg) genes in various nervous and muscular tissues at different stages of quail embryo d...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00335-x
更新日期：2000-07-01 00:00:00
abstract：:By means of subtractive and differential hybridization techniques we have identified a novel murine gene (1A13) the expression of which is developmentally regulated in the mouse brain. Comparison of the nucleotide and predicted protein sequence revealed closest relationship of 1A13 to human CoREST, a transcriptional c...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00477-4
更新日期：2001-10-01 00:00:00
abstract：:CD44, the major cell-surface receptor for hyaluronate, is expressed on many cell types to mediate different functions including cell activation, homing and adhesion. The early pattern of CD44 expression was determined in the avian embryo by using a specific monoclonal antibody in whole-mount and tissue sections. CD44 ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00347-6
更新日期：2000-08-01 00:00:00
abstract：:In this paper we analyze the expression in follicular cells and regulation of the vitelline membrane protein gene we identified in region 32E of the second chromosome of D. melanogaster (VMP32E). We report germ line transformation results obtained with different kinds of gene fusion leading to the identification of a ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(91)90018-2
更新日期：1991-11-01 00:00:00
abstract：:The embryonic fins and the tip of adult fins of teleost fish are supported by rows of straight, unmineralized fibrils called actinotrichia. The proximal ends of the actinotrichia are bundled and the mineralized bones called lepidotrichia are made along them. Since malformation in actinotrichia causes wavy fin bones, t...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2018.08.010
更新日期：2018-10-01 00:00:00
abstract：:We isolated and characterized embryonic lethal mutations in piragua (prg). The prg locus encodes a protein with an amino terminus Zinc Finger-Associated-Domain (ZAD) and nine C2H2 zinc fingers (ZF). prg mRNA and protein expression during embryogenesis is dynamic with widespread maternal contribution, and subsequent ex...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2016.12.003
更新日期：2017-04-01 00:00:00
abstract：:Increases in Na/K-ATPase activity occur concurrently with the onset of cavitation and are associated with increases in Na(+)-pump subunit mRNA and protein expression. We have hypothesized that the alpha1-isozyme of the Na/K-ATPase is required to mediate blastocyst formation. We have tested this hypothesis by character...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2004.04.005
更新日期：2004-05-01 00:00:00
abstract：:We isolated a novel gene by a differential-display RT-PCR method comparing basal disk tissue and peduncle tissue in a species of Hydra, Pelmatohydra robusta, and we referred to it as anklet. The putative anklet product has a signal sequence in its N-terminus, and it has one MAC/PF domain and one EGF domain. In normal ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2006.03.002
更新日期：2006-05-01 00:00:00
abstract：:The tumor-suppressor protein p53 belongs to a small gene family that includes p63 and p73. While p53 and p73 regulate cell cycle progression and apoptosis, the major role of p63 appears to be in promoting ectodermal proliferation and differentiation. In this report we describe the cloning of a Xenopus orthologue of ma...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00315-x
更新日期：2001-04-01 00:00:00
abstract：:In bovine preimplantation development, female embryos progress at lower rates and originate smaller blastocysts than male counterparts. Although sex-specific gene expression patterns are reported, when and how sex dimorphism is established is not clear. Differences among female and male early development can be useful...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2015.12.001
更新日期：2016-02-01 00:00:00
abstract：:Blood vessel maturation, which is characterized by the investment of vascular smooth muscle cells (vSMCs) around developing blood vessels, begins when vessels remodel into a hierarchy of proximal arteries and proximal veins that branch into smaller distal capillaries. The ultimate result of maturation is formation of ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2019.02.002
更新日期：2019-04-01 00:00:00
abstract：:The vertebrate hindbrain is composed of a series of lineage-restricted segments termed rhombomeres. Segment-specific gene expression drives unique programs of neuronal differentiation. Two critical embryonic signaling pathways, Fibroblast Growth Factor (FGF) and Retinoic Acid (RA), regulate early embryonic rhombomere ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2018.02.005
更新日期：2018-04-01 00:00:00
abstract：:We have characterised the transdifferentiation of human HepG2 (hepatoma) cells to pancreatic cells following introduction of an activated version of the pancreatic transcription factor Pdx1 (XlHbox8-VP16). The following questions are addressed: (1) are all types of pancreatic cells produced? (2) is the requirement for...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2005.01.001
更新日期：2005-06-01 00:00:00
abstract：:The process of gastrulation is a pivotal step in the formation of the vertebrate body plan. The primary function of gastrulation is the correct placement of precursor tissues for subsequent morphogenesis. There is now mounting evidence that the body plan is established through inductive interactions between germ layer...
journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/s0925-4773(97)00123-8
更新日期：1997-11-01 00:00:00
abstract：:Nuclear orphan receptors are DNA binding proteins that share the domain structure of the nuclear hormone receptor superfamily, although ligands are unknown. We have identified an orphan receptor in Xenopus laevis and named it xGCNF based on its high degree of sequence homology to the previously described murine germ c...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(96)00599-0
更新日期：1996-11-01 00:00:00
abstract：:To elucidate roles of fibroblast growth factors (FGF)18 during vertebrate development, we examined expression patterns of Fgf18 in chick embryos and observed effects of FGF18 protein on the Hensen's node, isthmus, and limb buds. Fgf18 is expressed on the right side of the node before the expression of Fgf8 starts. FGF...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00331-2
更新日期：2000-07-01 00:00:00
