Abstract：
:We isolated a novel gene by a differential-display RT-PCR method comparing basal disk tissue and peduncle tissue in a species of Hydra, Pelmatohydra robusta, and we referred to it as anklet. The putative anklet product has a signal sequence in its N-terminus, and it has one MAC/PF domain and one EGF domain. In normal hydra, the expression of anklet was restricted in the periphery of the basal disk and the lowest region of the peduncle. In foot-regenerating animals, anklet was first expressed in the newly differentiated basal disk gland cells at the regenerating basal end, and then expression became restricted at the periphery of the regenerated basal disk and in the lowest region of the peduncle. This spatially specific expression pattern suggested that the product of the anklet gene plays a role in basal disk formation. We therefore examined the role played by the protein product of the anklet gene by suppressing the transcription level of anklet using an RNA-mediated interference (RNAi) method. Suppression of the level of expression of the anklet gene led to a decrease in basal disk size in normal hydra, and to a delay in basal disk regeneration in foot-amputated animals. These results suggested that anklet is involved in the formation and maintenance of the basal disk in hydra.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Amimoto Y,Kodama R,Kobayakawa Ydoi
10.1016/j.mod.2006.03.002subject
Has Abstractpub_date
2006-05-01 00:00:00pages
352-61issue
5eissn
0925-4773issn
1872-6356pii
S0925-4773(06)00029-3journal_volume
123pub_type
杂志文章abstract：:Retinoic acid plays important roles in development, growth and differentiation by regulating the expression of target genes. A new retinoic acid-inducible gene, Stra6, has been identified in P19 embryonal carcinoma cells using a subtractive hybridization cDNA cloning technique. Stra6 codes for a very hydrophobic membr...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00039-7
更新日期：1997-05-01 00:00:00
abstract：:Avian neural crest cells migrate on precise pathways to their target areas where they form a wide variety of cellular derivatives, including neurons, glia, pigment cells and skeletal components. In one portion of their pathway, trunk neural crest cells navigate in the somitic mesoderm in a segmental fashion, invading ...
journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/s0925-4773(01)00395-1
更新日期：2001-07-01 00:00:00
abstract：:Tubby is a mouse gene that may provide a model for adult-onset obesity in humans. It is a member of a four gene family in mammals that collectively encode the Tubby-like proteins (TULPs), putative transcription factors which share similar 260 amino acid 'tubby domains' at their C-termini. The mammalian genome also enc...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(02)00211-3
更新日期：2002-09-01 00:00:00
abstract：:The notochord is a structure present in all chordates and its development requires the transcription of Brachyury. While previous studies have shown that Brachyury is essential for notochord formation in vertebrate embryos, this gene is also expressed during the embryogenesis of nonchordate deuterostomes, hemichordate...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00395-6
更新日期：2000-09-01 00:00:00
abstract：:We investigated the expression pattern of the endothelin-A receptor and endothelin 1 genes, the mutations of which affect the development of the mesectodermal derivatives of the neural crest. We show here that endothelin 1 is expressed by the environment of the cephalic neural crest cells invading branchial arches. La...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00079-3
更新日期：1998-07-01 00:00:00
abstract：:Ppp6c, which encodes the catalytic subunit of phosphoprotein phosphatase 6 (PP6), is conserved among eukaryotes from yeast to humans. In mammalian cells, PP6 targets IκBε for degradation, activates DNA-dependent protein kinase to trigger DNA repair, and is reportedly required for normal mitosis. Recently, Ppp6c mutati...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2016.02.001
更新日期：2016-02-01 00:00:00
abstract：:Early in the development of the imaginal wing disc of Drosophila, the LIM-HD gene tailup (islet), together with the HD genes of the iroquois complex, specify the notum territory of the disc. Later, tailup has been shown to act as a prepattern gene that antagonizes formation of sensory bristles on the notum of this fly...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2010.05.001
更新日期：2010-09-01 00:00:00
abstract：:The selective sensitivity of cells to programmed cell death (PCD) depends on the positive and negative death-inducing signals that converge into the apoptotic pathway. In Drosophila, the midline glial (MG) cells undergo selective death during development. Here, we show that the long isoform of the RNA-binding protein ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2008.10.004
更新日期：2009-01-01 00:00:00
abstract：:The phylogenetic location of hemichordates is unique because they seem to fill an evolutionary gap between echinoderms and chordates. We report here characterization of Pf-otx, a hemichordate ortholog of otx, with its embryonic and larval expression pattern. Pf-otx is initially expressed in the vegetal plate of the bl...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00279-8
更新日期：2000-03-01 00:00:00
abstract：:We studied the expression of MUL, a gene encoding a novel member of the RING-B-Box-Coiled Coil family of zinc finger proteins that underlies the human inherited disorder, Mulibrey nanism. In early human and mouse embryogenesis MUL is expressed in dorsal root and trigeminal ganglia, liver and in epithelia of multiple t...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00491-9
更新日期：2001-10-01 00:00:00
abstract：:We identified MRG-1, a Caenorhabditis elegans chromodomain-containing protein that is similar to the human mortality factor-related gene 15 product (MRG15). RNA-mediated interference (RNAi) of mrg-1 resulted in complete absence of the germline in both hermaphrodite and male adults. Examination of the expression of PGL...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(02)00058-8
更新日期：2002-06-01 00:00:00
abstract：:We previously showed that otx2 activates ectopic formation of the Xenopus cement gland only in ventrolateral ectoderm, defining a region of the embryo permissive for cement gland formation. In this paper, we explore the molecular identity of this permissive area. One candidate permissive factor is BMP4, whose putative...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00342-1
更新日期：2000-04-01 00:00:00
abstract：:Fetal liver is the major embryonic hematopoietic organ and is extrinsically colonized by circulating hematopoietic stem cells (HSCs). Integrin beta-1 expression on HSCs is crucial for colonization, suggesting that interaction of Integrin beta-1 with extra-cellular matrix (ECM) factors promotes HSC adherence to fetal l...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2012.09.003
更新日期：2013-02-01 00:00:00
abstract：:Homeodomain transcription factor CnNK-2 seems to play a major role in foot formation in Hydra. Recently, we reported in vitro evidence indicating that CnNK-2 has autoregulatory features and regulates expression of the morphogenetic peptide pedibin. We proposed that CnNK-2 and pedibin synergistically orchestrate foot d...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2005.04.010
更新日期：2005-09-01 00:00:00
abstract：:In endothelial cells that form capillary-like structures in vitro a variety of genes is upregulated as we have demonstrated previously. In addition to well known genes, we also identified genes never described in endothelial cells before. Here, we report the further characterization of one selected gene called cystein...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(02)00355-6
更新日期：2002-12-01 00:00:00
abstract：:The synchronous development of a sibling group of Rhynchosciara larvae enables us to follow the relationship between the local transcription and extrareplication of C3 and C8 DNA puffs. Although DNA amplification at these two loci takes place during the last cycle of DNA duplication in salivary gland chromosomes, a di...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(91)90072-e
更新日期：1991-12-01 00:00:00
abstract：:The Polycomb group genes in Drosophila are involved in the stable and inheritable repression of gene expression. The Polycomb group proteins probably operate as multimeric complexes that bind to chromatin. To investigate molecular mechanisms of stable repression of gene activity in vertebrates we have begun to study X...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(95)00422-x
更新日期：1995-09-01 00:00:00
abstract：:A new family of homeodomain proteins has recently been identified that includes extradenticle, ceh-20 and three mammalian proteins Pbx1, Pbx2 and Pbx3. We show here that two members of this family, Pbx1 and Pbx2 bind cooperatively to DNA with both Hoxb-7 and Hoxb-8. Engrailed-2 modulates the DNA binding activity of th...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(95)00394-g
更新日期：1995-07-01 00:00:00
abstract：:The human RUNX3/AML2 gene belongs to the 'runt domain' family of transcription factors that act as gene expression regulators in major developmental pathways. Here, we describe the expression pattern of Runx3 during mouse embryogenesis compared to the expression pattern of Runx1. E10.5 and E14.5-E16.5 embryos were ana...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00537-8
更新日期：2001-12-01 00:00:00
abstract：:Six/sine oculis (Six/so) class genes, with representatives in vertebrates and invertebrates, include members with key developmental roles in the anterior part of the central nervous system (CNS) and eye. Having characterized the role of the first planarian gene of the Six/so family in eye development, we attempted to ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(03)00111-4
更新日期：2002-12-01 00:00:00
abstract：:The POU domain is a approximately 140 amino acid domain shared by several mammalian transcriptional activators. We have isolated genomic and cDNA clones from three Drosophila POU domain genes, designated pdm-1, pdm-2, and Cf1a. All three genes encode a conserved POU-specific domain and a POU homeo domain but are other...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(91)90045-8
更新日期：1991-06-01 00:00:00
abstract：:The tetrapod vertebral column has become increasingly complex during evolution as an adaptation to a terrestrial life. At the same time, the evolution of the vertebral formula became subject to developmental constraints acting on the size of the cervical and thoraco-lumbar regions. In the course of our studies concern...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2015.07.006
更新日期：2015-11-01 00:00:00
abstract：:Recent work identified an apoptotic program in gastrulation stage Xenopus embryos (Anderson, J.A., Lewellyn, A.L., Maller, J.L., 1997. Mol. Biol. Cell 8, 1195-1206; Stack, J.H., Newport, J.W., 1997. Development 124, 3185-3195). Here, we characterize in detail this maternal cell death program, which is set up at fertil...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00191-3
更新日期：1997-12-01 00:00:00
abstract：:By means of subtractive and differential hybridization techniques we have identified a novel murine gene (1A13) the expression of which is developmentally regulated in the mouse brain. Comparison of the nucleotide and predicted protein sequence revealed closest relationship of 1A13 to human CoREST, a transcriptional c...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00477-4
更新日期：2001-10-01 00:00:00
abstract：:DCoH, the dimerization cofactor of the HNF-1 homeodomain proteins (hepatocyte nuclear factor-1alpha and beta), is involved in gene expression by associating with these transcription factors. The protein also called PCD for pterin-4alpha-carbinolamine dehydratase is a bifunctional factor as it catalyzes also the regene...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00269-5
更新日期：2000-03-01 00:00:00
abstract：:Cell fate specification during inner ear development is dependent upon regional gene expression within the otic vesicle. One of the earliest cell fate determination steps in this system is the specification of neural precursors, and regulators of this process include the Atonal-related basic helix-loop-helix genes, Ng...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2004.12.011
更新日期：2005-05-01 00:00:00
abstract：:The leucine zipper transcription factor TSC-22 (TGF-beta1 Stimulated Clone-22) was first isolated from a mouse osteoblast cell line as an immediate-early target gene of TGF-beta1. However, work with other cell lines, as well as with a Drosophila homolog, bunched, suggests that it is an effector gene of various growth ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00055-6
更新日期：1999-06-01 00:00:00
abstract：:The tumor-suppressor protein p53 belongs to a small gene family that includes p63 and p73. While p53 and p73 regulate cell cycle progression and apoptosis, the major role of p63 appears to be in promoting ectodermal proliferation and differentiation. In this report we describe the cloning of a Xenopus orthologue of ma...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00315-x
更新日期：2001-04-01 00:00:00
abstract：:The sex determining gene, Sry, determines the sex of the organism by initiating development of a testis rather than an ovary from the cells of the bipotential gonad. In the 10 years since the discovery of Sry, new genes and cellular pathways that operate in the establishment of the gonadal primordium and the initiatio...
journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/s0925-4773(99)00327-5
更新日期：2000-03-15 00:00:00
abstract：:The vascularization of the central nervous system occurs by angiogenic sprouting, a process in which different factors like vascular endothelial growth factor (VEGF) and angiopoietin (Ang)-1/2 must act in a coordinated fashion. We investigated how these factors participate in capillarization of the cerebellum, an area...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00471-3
更新日期：2001-10-01 00:00:00
