Enhanced sensitivity of midline glial cells to apoptosis is achieved by HOW(L)-dependent repression of Diap1.


:The selective sensitivity of cells to programmed cell death (PCD) depends on the positive and negative death-inducing signals that converge into the apoptotic pathway. In Drosophila, the midline glial (MG) cells undergo selective death during development. Here, we show that the long isoform of the RNA-binding protein Held Out Wing (HOW(L)) is essential for enhancing the sensitivity of the MG cells to PCD. In how mutant embryos, the number of MG cells was elevated. This phenotype could be rescued by midline expression of the HOW(L) repressor isoform. In how mutant embryos, the levels of the caspase inhibitor of apoptosis, Diap1 were elevated, in parallel to reduction in the levels of activated caspase. Similarly, reducing the levels of HOW in S2 cells led to elevation of Diap1, whereas over expression of HOW(L) promoted reduction of Diap1 protein as well as mRNA levels. Importantly, deletion of the two HOW binding sites from diap1 3'UTR abrogated HOW-dependent repression of Diap1, suggesting that HOW represses diap1 by binding to its 3'UTR. These results suggest that HOW(L) enhances the sensitivity of MG cells to apoptotic signals by reducing the levels of diap1 in these cells in, demonstrating a novel mode of regulation of PCD at the mRNA level.


Mech Dev


Reuveny A,Elhanany H,Volk T




Has Abstract


2009-01-01 00:00:00














  • Tissue distribution of PEBBLE RNA and pebble protein during Drosophila embryonic development.

    abstract::pebble (pbl) is required for cytokinesis during postblastoderm mitoses (Hime, G., Saint, R., 1992. Zygotic expression of the pebble locus is required for cytokinesis during the postblastoderm mitoses of Drosophila. Development 114, 165-171; Lehner, C.F., 1992. The pebble gene is required for cytokinesis in Drosophila....

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Prokopenko SN,Saint R,Bellen HJ

    更新日期:2000-02-01 00:00:00

  • In vitro transdifferentiation of hepatoma cells into functional pancreatic cells.

    abstract::We have characterised the transdifferentiation of human HepG2 (hepatoma) cells to pancreatic cells following introduction of an activated version of the pancreatic transcription factor Pdx1 (XlHbox8-VP16). The following questions are addressed: (1) are all types of pancreatic cells produced? (2) is the requirement for...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Li WC,Horb ME,Tosh D,Slack JM

    更新日期:2005-06-01 00:00:00

  • An octamer-binding site is crucial for the activity of an enhancer active at the embryonic met-/mesencephalic junction.

    abstract::An enhancer sequence found in the Protease Nexin-1 (PN-1) gene was shown to drive lacZ expression specifically at the met-/mesencephalic junction in transgenic mouse embryos. A functional study of this enhancer has been performed to better understand the mechanisms regulating isthmic gene expression. An octamer-bindin...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Mihailescu D,Küry P,Monard D

    更新日期:1999-06-01 00:00:00

  • Foot formation in Hydra: a novel gene, anklet, is involved in basal disk formation.

    abstract::We isolated a novel gene by a differential-display RT-PCR method comparing basal disk tissue and peduncle tissue in a species of Hydra, Pelmatohydra robusta, and we referred to it as anklet. The putative anklet product has a signal sequence in its N-terminus, and it has one MAC/PF domain and one EGF domain. In normal ...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Amimoto Y,Kodama R,Kobayakawa Y

    更新日期:2006-05-01 00:00:00

  • Zebrafish Hsp40 and Hsc70 genes are both induced during caudal fin regeneration.

    abstract::We report the characterization of a zebrafish DnaJ-like protein, homologue to the human Hsp40 protein hdj1, that we named zf-Hsp40. We have studied its expression during fin regeneration and we show that zf-Hsp40 mRNA level is drastically increased in all ray segments beneath the regenerated part, or blastema, in resp...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Tawk M,Joulie C,Vriz S

    更新日期:2000-12-01 00:00:00

  • Contribution of gastrin-releasing peptide and its receptor to villus development in the murine and human gastrointestinal tract.

    abstract::Recent studies have shown that aberrantly expressed gastrin-releasing peptide (GRP) and its receptor (GRP-R) critically regulate tumor cell differentiation in colon cancers developing in humans and mice. This finding suggested that the ability of GRP/GRP-R to promote a well-differentiated phenotype in colon cancer mig...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Carroll RE,Matkowskyj K,Saunthararajah Y,Sekosan M,Battey JF,Benya RV

    更新日期:2002-05-01 00:00:00

  • Hemodynamic force is required for vascular smooth muscle cell recruitment to blood vessels during mouse embryonic development.

    abstract::Blood vessel maturation, which is characterized by the investment of vascular smooth muscle cells (vSMCs) around developing blood vessels, begins when vessels remodel into a hierarchy of proximal arteries and proximal veins that branch into smaller distal capillaries. The ultimate result of maturation is formation of ...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Padget RL,Mohite SS,Hoog TG,Justis BS,Green BE,Udan RS

    更新日期:2019-04-01 00:00:00

  • Replication and transcription in the course of DNA amplification of the C3 and C8 DNA puffs of Rhynchosciara americana.

    abstract::The synchronous development of a sibling group of Rhynchosciara larvae enables us to follow the relationship between the local transcription and extrareplication of C3 and C8 DNA puffs. Although DNA amplification at these two loci takes place during the last cycle of DNA duplication in salivary gland chromosomes, a di...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Santelli RV,Machado-Santelli GM,Pueyo MT,Navarro-Cattapan LD,Lara FJ

    更新日期:1991-12-01 00:00:00

  • Gata2 and Gata3: novel markers for early embryonic polarity and for non-neural ectoderm in the chick embryo.

    abstract::We have investigated in detail the expression patterns of two Gata genes, cGata2 and cGata3, during early chick development. In addition to confirming previously described expression of these two genes in developing brain, kidney and blood islands, this study reveals several important novel expression domains during v...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Sheng G,Stern CD

    更新日期:1999-09-01 00:00:00

  • Stages of normal development in the medaka Oryzias latipes.

    abstract::Unfertilized eggs of Oryzias latipes were artificially inseminated and incubated at 26+/-1 degrees C. Careful observation of the process of embryonic development by light microscopy allowed division of the process into 39 stages based on diagnostic features of the developing embryos. The principal diagnostic features ...

    journal_title:Mechanisms of development

    pub_type: 杂志文章,评审


    authors: Iwamatsu T

    更新日期:2004-07-01 00:00:00

  • Modulation of Hedgehog target gene expression by the Fused serine-threonine kinase in wing imaginal discs.

    abstract::The Fused (Fu) serine-threonine kinase and the Suppressor of fused (Su(fu)) product are part of the Hedgehog (Hh) signaling pathway both in embryos and in imaginal discs. In wing imaginal discs, the Hh signal induces Cubitus interruptus (Ci) accumulation and activates patched (ptc) and decapentaplegic (dpp) expression...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Alves G,Limbourg-Bouchon B,Tricoire H,Brissard-Zahraoui J,Lamour-Isnard C,Busson D

    更新日期:1998-11-01 00:00:00

  • Tumor necrosis factor receptor associated factor 4 (TRAF4) expression pattern during mouse development.

    abstract::This is the first in situ hybridization analysis of expression of a tumor necrosis factor (TNF) receptor associated factor (TRAF) during development. TRAF4 is observed throughout mouse embryogenesis, most notably during ontogenesis of the central (CNS) and peripheral (PNS) nervous system, and of nervous tissues of sen...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Masson R,Régnier CH,Chenard MP,Wendling C,Mattei MG,Tomasetto C,Rio MC

    更新日期:1998-02-01 00:00:00

  • Origins of oligodendrocytes in the cerebellum, whose development is controlled by the transcription factor, Sox9.

    abstract::Development of oligodendrocytes, myelin-forming glia in the central nervous system (CNS), proceeds on a protracted schedule. Specification of oligodendrocyte progenitor cells (OPCs) begins early in development, whereas their terminal differentiation occurs at late embryonic and postnatal periods. However, for oligoden...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Hashimoto R,Hori K,Owa T,Miyashita S,Dewa K,Masuyama N,Sakai K,Hayase Y,Seto Y,Inoue YU,Inoue T,Ichinohe N,Kawaguchi Y,Akiyama H,Koizumi S,Hoshino M

    更新日期:2016-05-01 00:00:00

  • Interaction of gap genes in the Drosophila head: tailless regulates expression of empty spiracles in early embryonic patterning and brain development.

    abstract::Unlike gap genes in the trunk region of Drosophila embryos, gap genes in the head were presumed not to regulate each other's transcription. Here, we show that in tailless (tll) loss-of-function mutants the empty spiracles (ems) expression domain in the head expands, whereas it retracts in tll gain-of-function embryos....

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Hartmann B,Reichert H,Walldorf U

    更新日期:2001-12-01 00:00:00

  • Characterization of mouse Dach2, a homologue of Drosophila dachshund.

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    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Davis RJ,Shen W,Sandler YI,Heanue TA,Mardon G

    更新日期:2001-04-01 00:00:00

  • vHNF1 is expressed in epithelial cells of distinct embryonic origin during development and precedes HNF1 expression.

    abstract::HNF1 (Hepatic Nuclear Factor 1) and vHNF1 are transcriptional regulators containing a highly divergent homeodomain. The first was initially found in liver nuclear extracts and is crucial for the transcription of albumin and many other hepatocyte specific genes, while the second was found in dedifferentiated hepatoma c...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Ott MO,Rey-Campos J,Cereghini S,Yaniv M

    更新日期:1991-12-01 00:00:00

  • Co-localization of FAM and AF-6, the mammalian homologues of Drosophila faf and canoe, in mouse eye development.

    abstract::The Drosophila fat facets and canoe genes regulate non-neural cell fate decisions during ommatidium formation. We have shown previously that the FAM (fat facets in mouse) de-ubiquitinating enzyme regulates the function of AF-6, (mammalian canoe homologue), in the MDCK epithelial cell line (Taya et al., 1998. The Ras t...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Kanai-Azuma M,Mattick JS,Kaibuchi K,Wood SA

    更新日期:2000-03-01 00:00:00

  • Comparison of sequence and function of the Oct-6 genes in zebrafish, chicken and mouse.

    abstract::To examine the role of the Oct-6 gene in Schwann cell differentiation we have cloned and characterized the chicken and zebrafish homologues of the mouse Oct-6 gene. While highly homologous in the Pit1-Oct1/2-Unc86 (POU) domain, sequence similarities are limited outside this domain. Both genes are intronless and both p...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Levavasseur F,Mandemakers W,Visser P,Broos L,Grosveld F,Zivkovic D,Meijer D

    更新日期:1998-06-01 00:00:00

  • Oligomerisation of Tube and Pelle leads to nuclear localisation of dorsal.

    abstract::In the Drosophila embryo the nuclear localisation of Dorsal, a member of the Rel family, is regulated by an extracellular signal, which is transmitted to the interior of the egg cell by a cascade of proteins involving the novel protein Tube and the protein kinase Pelle. Here we analyse the activation mechanism of Tube...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Grosshans J,Schnorrer F,Nüsslein-Volhard C

    更新日期:1999-03-01 00:00:00

  • Sox neuro, a new Drosophila Sox gene expressed in the developing central nervous system.

    abstract::We describe the identification and detailed expression pattern of a second Drosophila Sox gene, SoxNeuro (SoxN), highly related to mammalian group B Sox1, 2, 3 genes. SoxN is expressed in a highly dynamic pattern during embyogenesis, being associated with the development of the central nervous system (CNS), from the e...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Crémazy F,Berta P,Girard F

    更新日期:2000-05-01 00:00:00

  • Xenopus Sox3 activates sox2 and geminin and indirectly represses Xvent2 expression to induce neural progenitor formation at the expense of non-neural ectodermal derivatives.

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    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Rogers CD,Harafuji N,Archer T,Cunningham DD,Casey ES

    更新日期:2009-01-01 00:00:00

  • The two Tribolium E(spl) genes show evolutionarily conserved expression and function during embryonic neurogenesis.

    abstract::Tribolium castaneum is a well-characterised model insect, whose short germ-band mode of embryonic development is characteristic of many insect species and differs from the exhaustively studied Drosophila. Mechanisms of early neurogenesis, however, show significant conservation with Drosophila, as a characteristic patt...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Kux K,Kiparaki M,Delidakis C

    更新日期:2013-04-01 00:00:00

  • An oncogenic form of human raf can specify terminal body pattern in Drosophila.

    abstract::Terminal portions of the Drosophila body pattern are specified by an extracellular ligand generated at each end of the early syncytial embryo. This ligand triggers the localized transcription of two gap segmentation genes, tailles (tll) and huckebein (hkb) through a signal transduction cascade involving the receptor t...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Casanova J,Llimargas M,Greenwood S,Struhl G

    更新日期:1994-10-01 00:00:00

  • N-CoR is required for patterning the anterior-posterior axis of zebrafish hindbrain by actively repressing retinoid signaling.

    abstract::Active repression of gene expression mediated by unliganded nuclear receptors plays crucial roles in early development of vertebrates. N-CoR (nuclear receptor co-repressor) is the first identified co-repressor that can repress retinoic acid (RA) inducible gene transcription in the absence of RA. Previously, N-CoR was ...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Xu F,Li K,Tian M,Hu P,Song W,Chen J,Gao X,Zhao Q

    更新日期:2009-10-01 00:00:00

  • A conserved 30 base pair element in the Wnt-5a promoter is sufficient both to drive its' early embryonic expression and to mediate its' repression by otx2.

    abstract::We have characterised a short (30 base pair) element from the Xenopus Wnt-5a promoter which is nearly identical to one located in the human Wnt-5a promoter, and has the same position relative to the transcription start site. When placed in front of a LacZ gene, this element can reproduce the same expression pattern ob...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Morgan R,Hooiveld MH,In der Reiden P,Durston AJ

    更新日期:1999-07-01 00:00:00

  • Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development.

    abstract::The vertebrate hindbrain is composed of a series of lineage-restricted segments termed rhombomeres. Segment-specific gene expression drives unique programs of neuronal differentiation. Two critical embryonic signaling pathways, Fibroblast Growth Factor (FGF) and Retinoic Acid (RA), regulate early embryonic rhombomere ...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Selland LG,Koch S,Laraque M,Waskiewicz AJ

    更新日期:2018-04-01 00:00:00

  • Epigenetic mechanisms and boundaries in the regulation of mammalian Hox clusters.

    abstract::Hox gene expression imparts segment identity to body structures along the anterior-posterior axis and is tightly governed by higher order chromatin mechanisms. Chromatin regulatory features of the homeotic complex are best defined in Drosophila melanogaster, where multiple cis-regulatory elements have been identified ...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Srivastava S,Dhawan J,Mishra RK

    更新日期:2015-11-01 00:00:00

  • Expression of CD44 during early development of the chick embryo.

    abstract::CD44, the major cell-surface receptor for hyaluronate, is expressed on many cell types to mediate different functions including cell activation, homing and adhesion. The early pattern of CD44 expression was determined in the avian embryo by using a specific monoclonal antibody in whole-mount and tissue sections. CD44 ...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Corbel C,Lehmann A,Davison F

    更新日期:2000-08-01 00:00:00

  • Xgravin-like (Xgl), a novel putative a-kinase anchoring protein (AKAP) expressed during embryonic development in Xenopus.

    abstract::A-kinase anchoring proteins (AKAPs) are a heterogeneous family of scaffolding proteins that regulate the compartmentalization of signaling components, in particular that of the broad specificity kinase PKA. Here we describe the identification of a new member of this gene family, termed Xenopus gravin-like (Xgl), which...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Klingbeil P,Frazzetto G,Bouwmeester T

    更新日期:2001-02-01 00:00:00

  • Differentiation of the epithelial apical junctional complex during mouse preimplantation development: a role for rab13 in the early maturation of the tight junction.

    abstract::We have investigated the mechanisms by which the epithelial apicolateral junctional complex (AJC) is generated during trophectoderm differentiation in the mouse blastocyst using molecular, structural and functional analyses. The mature AJC comprises an apical tight junction (TJ), responsible for intercellular sealing ...

    journal_title:Mechanisms of development

    pub_type: 杂志文章


    authors: Sheth B,Fontaine JJ,Ponza E,McCallum A,Page A,Citi S,Louvard D,Zahraoui A,Fleming TP

    更新日期:2000-10-01 00:00:00