Abstract:
:During the final stages of embryogenesis, the Drosophila embryo exhibits a dorsal hole covered by a simple epithelium of large cells termed the amnioserosa (AS). Dorsal closure is the process whereby this hole is closed through the coordination of cellular activities within both the AS and the epidermis. Genetic analysis has shown that signalling through Jun N-terminal Kinase (JNK) and Decapentaplegic (Dpp), a Drosophila member of the BMP/TGF-beta family of secreted factors, controls these activities. JNK activates the expression of dpp in the dorsal-most epidermal cells, and subsequently Dpp acts as a secreted signal to control the elongation of lateral epidermis. Our analysis shows that Dpp function not only affects the epidermal cells, but also the AS. Embryos defective in Dpp signalling display defects in AS cell shape changes, specifically in the reduction of their apical surface areas, leading to defective AS contraction. Our data also demonstrate that Dpp regulates adhesion between epidermis and AS, and mediates expression of the transcription factor U-shaped in a gradient across both the AS and the epidermis. In summary, we show that Dpp plays a crucial role in coordinating the activity of the AS and its interactions with the LE cells during dorsal closure.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Fernández BG,Arias AM,Jacinto Adoi
10.1016/j.mod.2007.09.002subject
Has Abstractpub_date
2007-11-01 00:00:00pages
884-97issue
11-12eissn
0925-4773issn
1872-6356pii
S0925-4773(07)00149-9journal_volume
124pub_type
杂志文章abstract::Formation of tubular structures from an epithelial tissue is a process common to many morphogenetic events during organogenesis. We report here new data concerning the expression pattern of the vHNF1/HNF1beta gene during this process in the mouse. vHNF1 (variant Hepatocyte Nuclear Factor 1) is a member of the HNF1 hom...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00221-x
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(96)00584-9
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00061-6
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
abstract::Myogenin and CMD1, the chicken homologue of MyoD, transactivate the promoter of the alpha-subunit of the acetylcholine receptor (AChR) in chicken fibroblasts. The expression of these three genes was followed by in situ hybridization. In two-day-old embryos the CMD1 gene is expressed shortly before the AChR alpha-subun...
journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2014.01.003
更新日期:2014-05-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00553-0
更新日期:2001-03-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00294-4
更新日期:2000-03-01 00:00:00
abstract::The tramtrack (ttk) gene of Drosophila encodes 69-kDa and 88-kDa proteins through alternative splicing of the primary ttk transcript. The two proteins share a common amino-terminal sequence, but contain different carboxy-terminal portions, each of which has a distinct zinc finger domain with a unique DNA binding speci...
journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00076-3
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2017.01.001
更新日期:2017-02-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(03)00091-1
更新日期:2002-12-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2003.12.001
更新日期:2004-02-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00009-4
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2008.01.008
更新日期:2008-05-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2005.04.010
更新日期:2005-09-01 00:00:00
abstract::Mutations in the CFTR gene cause cystic fibrosis (CF) with myocardial dysfunction. However, it remains unknown whether CF-related heart disease is a secondary effect of pulmonary disease, or an intrinsic primary defect in the heart. Here, we used zebrafish, which lack lung tissue, to investigate the role of CFTR in ca...
journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00079-3
更新日期:1998-07-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(03)00121-7
更新日期:2002-12-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章,评审
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2007.11.001
更新日期:2008-03-01 00:00:00
abstract::A new family of homeodomain proteins has recently been identified that includes extradenticle, ceh-20 and three mammalian proteins Pbx1, Pbx2 and Pbx3. We show here that two members of this family, Pbx1 and Pbx2 bind cooperatively to DNA with both Hoxb-7 and Hoxb-8. Engrailed-2 modulates the DNA binding activity of th...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(95)00394-g
更新日期:1995-07-01 00:00:00
abstract::Cadherins are Ca(+2)-dependent cell surface proteins involved in the specification of the adhesive properties of cells. They are supposed to play a critical role in morphogenesis and pattern formation. In this paper we show that in the sea urchin embryo there are at least two different cadherins of relative molecular ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(93)90054-2
更新日期:1993-04-01 00:00:00
abstract::The Suppressor of forked (Su(f)) protein of Drosophila melanogaster is a homologue of the 77K subunit of human cleavage stimulation factor required for cleavage of pre-mRNAs before addition of poly(A). We have previously shown that the Su(f) protein is not ubiquitously distributed: it accumulates in dividing cells at ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00011-8
更新日期:1999-04-01 00:00:00