Abstract：
:Broad (br), a transcription factor containing the Broad-Tramtrack-Bric-a-brac (BTB) and zinc finger domains was shown to mediate 20-hydroxyecdysone (20E) action and pupal development in Drosophila melanogaster and Manduca sexta. We determined the key roles of br during larval-pupal metamorphosis using RNA interference (RNAi) in a coleopteran insect, Tribolium castaneum. Two major peaks of T. castaneum broad (Tcbr) mRNA, one peak at the end of feeding stage prior to the larvae entering the quiescent stage and another peak during the quiescent stage were detected in the whole body and midgut tissue dissected from staged insects. Expression of br during the final instar larval stage is essential for successful larval-pupal metamorphosis, because, RNAi-mediated knock-down of Tcbr during this stage derailed larval-pupal metamorphosis and produced insects that showed larval, pupal and adult structures. Tcbr dsRNA injected into the final instar larvae caused reduction in the mRNA levels of genes known to be involved in 20E action (EcRA, E74 and E75B). Tcbr dsRNA injected into the final instar larvae also caused an increase in the mRNA levels of JH-response genes (JHE and Kr-h1b). Knock-down of Tcbr expression also affected 20E-mediated remodeling of midgut during larval-pupal metamorphosis. These data suggest that the expression of Tcbr during the final instar larval stage promotes pupal program while suppressing the larval and adult programs ensuring a transitory pupal stage in holometabolous insects.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Parthasarathy R,Tan A,Bai H,Palli SRdoi
10.1016/j.mod.2007.11.001subject
Has Abstractpub_date
2008-03-01 00:00:00pages
299-313issue
3-4eissn
0925-4773issn
1872-6356pii
S0925-4773(07)00196-7journal_volume
125pub_type
杂志文章abstract：:The products of the trithorax gene are required to stably maintain homeotic gene expression patterns established during embryo-genesis by the action of the transiently expressed segmentation genes. We have determined the intron/exon structure of the trx gene and the large alternatively spliced trx RNAs, which are capa...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(95)00402-m
更新日期：1995-08-01 00:00:00
abstract：:The p53 family network is a unique cellular processor that integrates information from various pathways and determines cellular choices between proliferation, replication arrest/repair, differentiation, senescence, or apoptosis. The most studied role of the p53 family is the regulation of stress response and tumor sup...
journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/j.mod.2008.09.003
更新日期：2008-11-01 00:00:00
abstract：:We describe the identification and detailed expression pattern of a second Drosophila Sox gene, SoxNeuro (SoxN), highly related to mammalian group B Sox1, 2, 3 genes. SoxN is expressed in a highly dynamic pattern during embyogenesis, being associated with the development of the central nervous system (CNS), from the e...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00268-9
更新日期：2000-05-01 00:00:00
abstract：:Dystroglycan is a transmembrane receptor protein that provides a structural linkage between extracellular matrix components and cytoskeletal proteins. It was originally characterized as a member of dystrophin associated protein complex in muscle but, unlike other proteins of this complex, mutations in the dystroglycan...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(03)00091-1
更新日期：2002-12-01 00:00:00
abstract：:The zebrafish biklf gene encodes a novel Krüppel-like transcription factor containing three contiguous zinc fingers at the C-terminus. Expression of biklf is detected from the shield stage onward in the developing prechordal plate, and as a 'baseball seam'-like lateral stripe beginning at the end of gastrulation. The ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00404-4
更新日期：2000-10-01 00:00:00
abstract：:ADAM19 is a member of the meltrin subfamily of ADAM metalloproteases. In Xenopus, ADAM19 is present as a maternal transcript. Zygotic expression starts during gastrulation and is apparent in the dorsal blastopore lip. ADAM19 expression through neurulation and tailbud formation becomes enriched in dorsal structures suc...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2008.10.010
更新日期：2009-03-01 00:00:00
abstract：:By means of subtractive and differential hybridization techniques we have identified a novel murine gene (1A13) the expression of which is developmentally regulated in the mouse brain. Comparison of the nucleotide and predicted protein sequence revealed closest relationship of 1A13 to human CoREST, a transcriptional c...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00477-4
更新日期：2001-10-01 00:00:00
abstract：:It is now well established that apoptosis is induced in response to mechanical strain. Indeed, increasing compressive forces induces apoptosis in confined spheroids of tumour cells, whereas releasing stress reduces apoptosis in spheroids cultivated in free suspension (Cheng et al., 2009). Apoptosis can also be induced...
journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/j.mod.2016.10.001
更新日期：2017-04-01 00:00:00
abstract：:The Ras protein activates at least three different pathways during early development. Two of them regulate mesodermal gene expression and the third is thought to participate in the control of actin cytoskeleton dynamics via the Ral protein. From a yeast two-hybrid screen of a Xenopus maternal cDNA library, we identifi...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2004.07.008
更新日期：2004-12-01 00:00:00
abstract：:The maintenance of pluripotential embryonic stem (ES) cells is dependent on the cytokine LIF. This report documents the mRNA expression profiles of LIF and the two components of the LIF-receptor complex, LIF-R and gp130, during early mouse embryogenesis. These mRNAs were undetectable in 1- or 2-cell embryos, but all w...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(96)00531-x
更新日期：1996-07-01 00:00:00
abstract：:Tribolium castaneum is a well-characterised model insect, whose short germ-band mode of embryonic development is characteristic of many insect species and differs from the exhaustively studied Drosophila. Mechanisms of early neurogenesis, however, show significant conservation with Drosophila, as a characteristic patt...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2013.02.003
更新日期：2013-04-01 00:00:00
abstract：:Proper metazoan mesoderm development requires the function of a basic helix-loop-helix (bHLH) transcription factor, Twist. Twist-containing dimers regulate the expression of target genes by binding to E box promoter elements containing the site CANNTG. In Caenorhabditis elegans, CeTwist functions in a subset of mesode...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2007.01.005
更新日期：2007-05-01 00:00:00
abstract：:A model of the trans-regulation of the Drosophila pair-rule segmentation gene, paired (prd), has been derived from the observed alterations in the distribution of prd transcripts during early embryogenesis in single and double pair-rule mutants. Important aspects of the model include the following features and implica...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(90)90130-e
更新日期：1990-12-01 00:00:00
abstract：:Many proteins involved in signal transduction and cell adhesion are characterized by the presence of an extracellular domain with repeated copies of a leucine-rich motif (LRR). Here we report the isolation and characterization of a novel gene, tlr (for Toll-like receptor), which encodes a protein containing multiple L...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(94)90041-8
更新日期：1994-09-01 00:00:00
abstract：:We have made a null mutation in the mouse activin beta B gene by deleting the portion of the gene encoding the proteolytic cleavage site and the majority of the coding region for the mature processed protein. Mice homozygous for this mutation complete embryogenesis and are completely viable. Approximately 40% of the h...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(94)90094-9
更新日期：1994-07-01 00:00:00
abstract：:The actin cytoskeleton is implicated in many cellular processes, such as cell adhesion, locomotion, contraction and cytokinesis, which are central to any development. The extent of polymerization, cross-linking, and bundling of actin is regulated by several actin-binding proteins. Knock-out mutations in these proteins...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00292-0
更新日期：2000-03-01 00:00:00
abstract：:Mll, Brg1 and Brm are vertebrate homologues of Drosophila trithorax group (trxG) genes. We isolated chicken Mll cDNA clones, and examined patterns of Mll, Brg1 and Brm expression in chick embryos. All three genes were expressed from embryonic stage 2 onwards. Mll transcripts were just detectable in all tissues by in s...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00207-x
更新日期：1999-01-01 00:00:00
abstract：:The vertebrate neural tube develops by two distinct mechanisms. Anteriorly, in the brain and future trunk (cervicothoracic) region, 'primary neurulation' occurs, where an epithelial sheet rolls or bends into a tube. Posteriorly, in the future lumbar and tail region, the neural tube forms by 'secondary neurulation', wh...
journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/j.mod.2004.04.022
更新日期：2004-10-01 00:00:00
abstract：:We describe the structural organization of a gene, termed XFDL 141/156, that is transiently activated during early Xenopus development. XFDL 141/156 is first transcribed at the midblastula transition (MBT) and during early gastrulation events. A roughly 200 nucleotide fragment immediately 5' to the transcription start...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00034-8
更新日期：1997-04-01 00:00:00
abstract：:The idea has been put forward that molecules and mechanisms acting during development are re-used during regeneration in the adult, for example in response to traumatic injury in order to re-establish the functional integrity of neuronal circuits. Members of the Eph family of receptor tyrosine kinases and their 'ligan...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00431-2
更新日期：2001-08-01 00:00:00
abstract：:Directional migration of primordial germ cells (PGCs) toward future gonads is a common feature in many animals. In zebrafish, mouse and chicken, SDF-1/CXCR4 chemokine signaling has been shown to have an important role in PGC migration. In Xenopus, SDF-1 is expressed in several regions in embryos including dorsal mesod...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2009.09.005
更新日期：2010-01-01 00:00:00
abstract：:Using a Drosophila cell-free system, we have analyzed the regulation of alternative splicing of Drosophila muscle myosin heavy chain (MHC) transcripts. Splicing of MHC 3' end transcripts results in exclusion of adult-specific alternative exon 18, as is observed in embryonic and larval muscle in vivo. Mutations that st...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(92)90075-u
更新日期：1992-05-01 00:00:00
abstract：:The transcription factor scleraxis (SCX) is expressed throughout tendon development and plays a key role in directing tendon wound healing. However, little is known regarding its role in fetal or young postnatal tendons, stages in development that are known for their enhanced regenerative capabilities. Here we used RN...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2020.103635
更新日期：2020-09-01 00:00:00
abstract：:In Drosophila melanogaster, the patterning of dorsal appendages on the eggshell is strictly controlled by EGFR signaling. However, the number of dorsal appendages is remarkably diverse among Drosophila species. For example, D. melanogaster and D. virilis have two and four dorsal appendages, respectively. Here we show ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(03)00164-3
更新日期：2003-08-01 00:00:00
abstract：:The gastrointestinal tract is subdivided into regions with different roles in digestion and absorption. How this patterning is established is unknown. We now report that the pancreatic-duodenal homeobox 1 gene (pdx1) is also expressed in cells of the distal stomach. Positive cells include subpopulations of the three m...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(96)00609-0
更新日期：1996-12-01 00:00:00
abstract：:Lipids, in addition to being structural components of cell membranes, can act as signaling molecules. Bioactive lipids, such as sphingosine 1-phosphate (S1P) and lysophosphatidic acid (LPA), may act intracellularly as second messengers or be secreted and act as intercellular signaling molecules. Such molecules can aff...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(03)00131-x
更新日期：2002-12-01 00:00:00
abstract：:We report the characterization of a zebrafish DnaJ-like protein, homologue to the human Hsp40 protein hdj1, that we named zf-Hsp40. We have studied its expression during fin regeneration and we show that zf-Hsp40 mRNA level is drastically increased in all ray segments beneath the regenerated part, or blastema, in resp...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00478-0
更新日期：2000-12-01 00:00:00
abstract：:In a screen for receptor tyrosine kinase genes regionally expressed in the developing hindbrain, we cloned and characterised the chicken ret gene. Data derived from studies of congenital human disease and from disruption of murine ret have demonstrated roles for ret in development of the kidney and enteric nervous sys...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(95)00449-1
更新日期：1995-11-01 00:00:00
abstract：:Unlike gap genes in the trunk region of Drosophila embryos, gap genes in the head were presumed not to regulate each other's transcription. Here, we show that in tailless (tll) loss-of-function mutants the empty spiracles (ems) expression domain in the head expands, whereas it retracts in tll gain-of-function embryos....
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00519-6
更新日期：2001-12-01 00:00:00
abstract：:An important question in oocyte-mediated nuclear reprogramming is whether gene expression of the donor nucleus changes randomly or follows a pattern. Since cloned embryos are very heterogeneous and arrest frequently during preimplantation development, a random scenario is generally accepted. In the present study, we r...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2007.09.007
更新日期：2008-01-01 00:00:00
