Abstract:
:The actin cytoskeleton is implicated in many cellular processes, such as cell adhesion, locomotion, contraction and cytokinesis, which are central to any development. The extent of polymerization, cross-linking, and bundling of actin is regulated by several actin-binding proteins. Knock-out mutations in these proteins have revealed in many cases only subtle, if any, defects in development, suggesting that the actin system is redundant, with multiple proteins sharing overlapping functions. The apparent redundancy may, however, reflect limitations of available laboratory assays in assessing the developmental role of a given protein. By using a novel assay, which reproduces conditions closer to the natural ones, we have re-examined the effects of disruption of many actin-binding proteins, and show here that deletion of alpha-actinin, interaptin, synexin, 34-kDa actin-bundling protein, and gelation factor affect to varying degrees the efficiency of Dictyostelium cells to complete development and form viable spores. No phenotypic defects were found in hisactophilin or comitin null mutants.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Ponte E,Rivero F,Fechheimer M,Noegel A,Bozzaro Sdoi
10.1016/s0925-4773(99)00292-0subject
Has Abstractpub_date
2000-03-01 00:00:00pages
153-61issue
1-2eissn
0925-4773issn
1872-6356pii
S0925-4773(99)00292-0journal_volume
91pub_type
杂志文章abstract::Five chick homeodomain proteins (CHOXs), CHOX-1.7, -1.1, -1.4, -4.2 and -2.6, had different transcription-regulating activities in a chick cultured cell line, LMH. In particular, CHOX-1.7 highly activated transcription when NP6 was used as the target site whereas CHOX-1.4 did not. This was mainly due to differences in...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(92)90012-9
更新日期:1992-03-01 00:00:00
abstract::Transforming growth factor beta 2 (TGF-beta2) plays a critical role in growth, differentiation and cell death, but its function in the developing cerebellum is still uncertain. In this study we analyzed the effects of TGF-beta2 on ex vivo developing cerebellar slice cultures. Proliferation of granule cell precursors p...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2004.10.012
更新日期:2005-04-01 00:00:00
abstract::The full length sequence of the Xenopus integrin alpha 5 subunit is reported. Analysis of cloned cDNA fragments reveals that alternative polyadenylation of alpha 5 mRNA occurs in the embryo. Furthermore, a variant form of the alpha 5 mRNA is expressed which encodes an integrin alpha 5 subunit with a truncated cytoplas...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(94)00335-k
更新日期:1995-04-01 00:00:00
abstract::An important question in oocyte-mediated nuclear reprogramming is whether gene expression of the donor nucleus changes randomly or follows a pattern. Since cloned embryos are very heterogeneous and arrest frequently during preimplantation development, a random scenario is generally accepted. In the present study, we r...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2007.09.007
更新日期:2008-01-01 00:00:00
abstract::In mouse Otx2 plays essential roles in anterior-posterior axis formation and head development in anterior visceral endoderm and anterior mesendoderm. The Otx2 expression in these sites is regulated by VE and CM enhancers at the 5' proximal to the translation start site, and we proposed that these enhancers would have ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2011.11.001
更新日期:2012-01-01 00:00:00
abstract::We performed a systematic screen for mutations affecting the trajectory of axons visualized by immunohistochemical staining of Medaka embryos with anti-acetylated tubulin antibody. Among the mutations identified, yanagi (yan) and kazura (kaz) mutations caused specific defects in projection of the posterior lateral lin...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2004.03.032
更新日期:2004-07-01 00:00:00
abstract::The Notch effector E(spl)M8 is phosphorylated at Ser159 by CK2, a highly conserved Ser/Thr protein kinase. We have used the Gal4-UAS system to assess the role of M8 phosphorylation during bristle and eye morphogenesis by employing a non-phosphorylatable variant (M8SA) or one predicted to mimic the 'constitutively' pho...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2004.01.008
更新日期:2004-03-01 00:00:00
abstract::The Mix/Bix family of Pax-like homeodomain transcription factors is expressed early in vertebrate development and play important roles in endoderm and mesoderm formation. Like other Pax-related homeodomain proteins, the Mix/Bix family binds DNA as monomers or dimers and dimerization is mediated by the homeodomain. Whi...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2005.09.006
更新日期:2006-01-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00451-2
更新日期:2000-11-01 00:00:00
abstract::Haploid spermatids undergo extensive cellular, molecular and morphological changes to form spermatozoa during spermiogenesis. Abnormalities in these steps can lead to serious male fertility problems, from oligospermia to complete azoospermia. CHD5 is a chromatin-remodeling nuclear protein expressed almost exclusively ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2013.10.005
更新日期:2014-02-01 00:00:00
abstract::The gene tprd, which contains three tetratricopeptide domains, has been recently localized in the Down syndrome (DS) chromosomal region 1. We have cloned a cDNA encoding part of the murine ortholog of tprd and used it to characterize the expression pattern of this gene during development and at the adult stage. At E8....
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00076-3
更新日期:1999-06-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(02)00220-4
更新日期:2002-10-01 00:00:00
abstract::The notochord is a structure present in all chordates and its development requires the transcription of Brachyury. While previous studies have shown that Brachyury is essential for notochord formation in vertebrate embryos, this gene is also expressed during the embryogenesis of nonchordate deuterostomes, hemichordate...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00395-6
更新日期:2000-09-01 00:00:00
abstract::Cadherins are Ca(+2)-dependent cell surface proteins involved in the specification of the adhesive properties of cells. They are supposed to play a critical role in morphogenesis and pattern formation. In this paper we show that in the sea urchin embryo there are at least two different cadherins of relative molecular ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(93)90054-2
更新日期:1993-04-01 00:00:00
abstract::To examine the role of the Oct-6 gene in Schwann cell differentiation we have cloned and characterized the chicken and zebrafish homologues of the mouse Oct-6 gene. While highly homologous in the Pit1-Oct1/2-Unc86 (POU) domain, sequence similarities are limited outside this domain. Both genes are intronless and both p...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00067-7
更新日期:1998-06-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00009-4
更新日期:1998-02-01 00:00:00
abstract::We have isolated and characterized two complete cDNA clones, Zfz8a and Zfz8b, which encode zebrafish Frizzled (Fz) homologues. The predicted protein sequences, spanning 579 and 576 amino acid residues for ZFz8a and ZFz8b, respectively, were highly homologous (78%) to each other and contained an extracellular cysteine-...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00137-3
更新日期:1998-11-01 00:00:00
abstract::X-chromosome inactivation (XCI) is an epigenetic mechanism that occurs in the eutherian embryo development to equalize the dosage of X-linked genes between males and females. This event is regulated by various factors, and the genes located in the X-chromosome inactivation center (XIC), which is known to be an evoluti...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2015.10.005
更新日期:2015-11-01 00:00:00
abstract::Drosophila immunity and embryogenesis appear to be linked by an evolutionarily ancient signalling pathway, which includes the Rel-domain transcription factors Dif and dorsal, respectively, as well as a common inhibitor, cactus. Previous genetic screens have centered on maternal mutants that disrupt the dorsal pathway....
journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00351-3
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2012.05.001
更新日期:2012-07-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(97)00065-8
更新日期:1997-07-01 00:00:00
abstract::The Bar homeobox genes play an essential role during nervous system and eye development in Drosophila. We isolated a medaka Bar gene closely related to the Drosophila and mammalian Bar genes. In the medaka embryo, OlBar is expressed from gastrula stages onwards, in a region demarcating the presumptive prosencephalic-m...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(02)00054-0
更新日期:2002-06-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2016.02.004
更新日期:2016-05-01 00:00:00
abstract::The recent determination and annotation of the entire euchromatic sequence of the Drosophila melanogaster genome predicted the existence of about 13600 different genes (Science 287 (2000) 2185; http://www.fruitfly.org/annot/index.html). In parallel, the Berkeley Drosophila Genome Project (BDGP) has undertaken systemat...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00566-4
更新日期:2002-01-01 00:00:00
abstract::In a screen for receptor tyrosine kinase genes regionally expressed in the developing hindbrain, we cloned and characterised the chicken ret gene. Data derived from studies of congenital human disease and from disruption of murine ret have demonstrated roles for ret in development of the kidney and enteric nervous sys...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(95)00449-1
更新日期:1995-11-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2013.09.005
更新日期:2014-02-01 00:00:00
abstract::In many animals, germline progenitors are kept undifferentiated to give rise to germline stem cells (GSCs), enabling continuous production of gametes throughout animal life. In the Drosophila ovary, GSCs arise from a subset of primordial germ cells (PGCs) that stay undifferentiated even after gametogenesis has started...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2013.01.002
更新日期:2013-04-01 00:00:00
abstract::The enteric nervous system (ENS) derives from migratory neural crest cells that colonize the developing gut tube, giving rise to an integrated network of neurons and glial cells, which together regulate important aspects of gut function, including coordinating the smooth muscle contractions of the gut wall. The absenc...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2008.04.006
更新日期:2008-08-01 00:00:00