Abstract：
:The Notch effector E(spl)M8 is phosphorylated at Ser159 by CK2, a highly conserved Ser/Thr protein kinase. We have used the Gal4-UAS system to assess the role of M8 phosphorylation during bristle and eye morphogenesis by employing a non-phosphorylatable variant (M8SA) or one predicted to mimic the 'constitutively' phosphorylated protein (M8SD). We find that phosphorylation of M8 does not appear to be critical during bristle morphogenesis. In contrast, only M8SD elicits a severe 'reduced eye' phenotype when it is expressed in the morphogenetic furrow of the eye disc. M8SD elicits neural hypoplasia in eye discs, elicits loss of phase-shifted Atonal-positive cells, i.e. the 'founding' R8 photoreceptors, and consequently leads to apoptosis. The ommatidial phenotype of M8SD is similar to that in Nspl/Y; E(spl)D/+ flies. E(spl)D, an allele of m8, encodes a truncated protein known as M8*, which, unlike wild type M8, displays exacerbated antagonism of Atonal via direct protein-protein interactions. In line with this, we find that the M8SD-Atonal interaction appears indistinguishable from that of M8*-Atonal, whereas interaction of M8 or M8SA appears marginal, at best. These results raise the possibility that phosphorylation of M8 (at Ser159) might be required for its ability to mediate 'lateral inhibition' within proneural clusters in the developing retina. This is the first identification of a dominant allele encoding a phosphorylation-site variant of an E(spl) protein. Our studies uncover a novel functional domain that is conserved amongst a subset of E(spl)/Hes repressors in Drosophila and mammals, and suggests a potential role for CK2 during retinal patterning.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Karandikar UC,Trott RL,Yin J,Bishop CP,Bidwai APdoi
10.1016/j.mod.2004.01.008subject
Has Abstractpub_date
2004-03-01 00:00:00pages
273-86issue
3eissn
0925-4773issn
1872-6356pii
S0925477304000206journal_volume
121pub_type
杂志文章abstract：:In the last years, several reports have established the notion that metabolism is not just a housekeeping process, but instead an active effector of physiological changes. The idea that the metabolic status may rule a wide range of phenomena in cell biology is starting to be broadly accepted. Thus, current development...
journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/j.mod.2018.02.004
更新日期：2018-12-01 00:00:00
abstract：:DmFoxF is a novel Drosophila fork head domain factor, which is expressed in the visceral mesoderm of the embryo. Our data suggest that DmFoxF is the fly orthologue of the vertebrates FOXF1 and FOXF2 transcription factors. DmFoxF shares homology with FOXF1 and FOXF2 in its fork head domain, and it is able to specifical...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00603-7
更新日期：2002-02-01 00:00:00
abstract：:We have used whole mount in situ hybridization to analyze the pattern of expression of the gene Endo 16 in S. purpuratus embryos. The mRNA is first detectable at 18 h post-fertilization in the cytoplasm of blastomeres derived from the Veg2 6th cleavage tier. The number of Endo 16 positive cells increases gradually thr...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(93)90001-e
更新日期：1993-08-01 00:00:00
abstract：:The tetrapod vertebral column has become increasingly complex during evolution as an adaptation to a terrestrial life. At the same time, the evolution of the vertebral formula became subject to developmental constraints acting on the size of the cervical and thoraco-lumbar regions. In the course of our studies concern...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2015.07.006
更新日期：2015-11-01 00:00:00
abstract：:Formation of tubular structures from an epithelial tissue is a process common to many morphogenetic events during organogenesis. We report here new data concerning the expression pattern of the vHNF1/HNF1beta gene during this process in the mouse. vHNF1 (variant Hepatocyte Nuclear Factor 1) is a member of the HNF1 hom...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00221-x
更新日期：1999-12-01 00:00:00
abstract：:BMP2, like its Drosophila homologue dpp, is an important signaling molecule for specification of cardiogenic mesoderm in vertebrates. Here, we analyzed the time-course of BMP2-requirement for early heart formation in whole chick embryos and in explants of antero-lateral plate mesoderm. Addition of Noggin to explants i...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00311-1
更新日期：2000-03-01 00:00:00
abstract：:Cell-cell interactions during embryogenesis have been shown to be important for establishing developmental fates in a number of organisms such as Drosophila and Caenorhabditis. One class of genes shown to mediate this process are receptor-protein tyrosine kinases (R-PTKs). To examine whether R-PTKs might participate i...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(94)90078-7
更新日期：1994-05-01 00:00:00
abstract：:The foot of the simple metazoan Hydra is a highly dynamic body region of constant tissue movement, cell proliferation, determination and differentiation. Previously, two genes have been shown to participate in the development and differentiation of this body region: homeodomain factor CnNK-2 and signal peptide pedibin...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2003.12.001
更新日期：2004-02-01 00:00:00
abstract：:Active repression of gene expression mediated by unliganded nuclear receptors plays crucial roles in early development of vertebrates. N-CoR (nuclear receptor co-repressor) is the first identified co-repressor that can repress retinoic acid (RA) inducible gene transcription in the absence of RA. Previously, N-CoR was ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2009.09.001
更新日期：2009-10-01 00:00:00
abstract：:We have identified a novel chicken gene, cMespo, which encodes a basic-helix-loop-helix (bHLH) protein with sequence homology to a subgroup of bHLH transcription factors that have been implicated in somitogenesis. cMespo transcripts are first found in the primitive streak of gastrulating chick embryos (HH stage 4) and...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00424-x
更新日期：2000-10-01 00:00:00
abstract：:The Drosophila fat facets and canoe genes regulate non-neural cell fate decisions during ommatidium formation. We have shown previously that the FAM (fat facets in mouse) de-ubiquitinating enzyme regulates the function of AF-6, (mammalian canoe homologue), in the MDCK epithelial cell line (Taya et al., 1998. The Ras t...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00294-4
更新日期：2000-03-01 00:00:00
abstract：:The enteric nervous system (ENS) derives from migratory neural crest cells that colonize the developing gut tube, giving rise to an integrated network of neurons and glial cells, which together regulate important aspects of gut function, including coordinating the smooth muscle contractions of the gut wall. The absenc...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2008.04.006
更新日期：2008-08-01 00:00:00
abstract：:Broad (br), a transcription factor containing the Broad-Tramtrack-Bric-a-brac (BTB) and zinc finger domains was shown to mediate 20-hydroxyecdysone (20E) action and pupal development in Drosophila melanogaster and Manduca sexta. We determined the key roles of br during larval-pupal metamorphosis using RNA interference...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2007.11.001
更新日期：2008-03-01 00:00:00
abstract：:The odd-skipped (odd) gene encodes a zinc finger protein that represses other segmentation genes in the early Drosophila embryo. Though odd is initially expressed in a striped pattern that reflects its function within the segmentation hierarchy, it is also expressed in a variety of patterns during later stages of embr...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00389-0
更新日期：2000-09-01 00:00:00
abstract：:Basic helix-loop-helix (bHLH) proteins of the Achaete/Scute (Ac/Sc) family are required for neurogenesis in both Drosophila and vertebrates. These transcription factors are commonly referred to as 'proneural' factors, as they promote neural fate in many contexts. Although Ac/Sc proteins have been studied in Hydra, jel...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2008.06.002
更新日期：2008-09-01 00:00:00
abstract：:We report the characterization of a zebrafish DnaJ-like protein, homologue to the human Hsp40 protein hdj1, that we named zf-Hsp40. We have studied its expression during fin regeneration and we show that zf-Hsp40 mRNA level is drastically increased in all ray segments beneath the regenerated part, or blastema, in resp...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00478-0
更新日期：2000-12-01 00:00:00
abstract：:Deflecting biomineralized crystals attached to vestibular hair cells are necessary for maintaining balance. Zebrafish (Danio rerio) are useful organisms to study these biomineralized crystals called otoliths, as many required genes are homologous to human otoconial development. We sought to identify and characterize t...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2019.04.001
更新日期：2019-06-01 00:00:00
abstract：:T-box genes constitute a conserved multi-gene family with important roles in many developmental processes. In this report, we describe the cloning and expression analysis of a novel mouse T-box gene, Tbx20. Expression is prominent in the extraembryonic mesoderm, in the developing heart, the eye anlage and motor neuron...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00499-8
更新日期：2001-01-01 00:00:00
abstract：:Mitochondrial large rRNA (mtlrRNA) and small rRNA (mtsrRNA) have been identified as components of germinal granules in Drosophila. We have previously reported that mtlrRNA is present on the germinal granules in Xenopus embryos. Here we report that mtsrRNA is also a common component of the germinal granules. Extra-mito...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00553-0
更新日期：2001-03-01 00:00:00
abstract：:C-terminal binding protein (CtBP) is an evolutionarily and functionally conserved transcriptional corepressor known to integrate diverse signals to regulate transcription. Drosophila CtBP (dCtBP) regulates tissue specification and segmentation during early embryogenesis. Here, we investigated the roles of dCtBP during...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2008.10.003
更新日期：2009-01-01 00:00:00
abstract：:A model of the trans-regulation of the Drosophila pair-rule segmentation gene, paired (prd), has been derived from the observed alterations in the distribution of prd transcripts during early embryogenesis in single and double pair-rule mutants. Important aspects of the model include the following features and implica...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(90)90130-e
更新日期：1990-12-01 00:00:00
abstract：:Terminal portions of the Drosophila body pattern are specified by an extracellular ligand generated at each end of the early syncytial embryo. This ligand triggers the localized transcription of two gap segmentation genes, tailles (tll) and huckebein (hkb) through a signal transduction cascade involving the receptor t...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(94)90006-x
更新日期：1994-10-01 00:00:00
abstract：:In Xenopus and zebrafish, BMP2, 4 and 7 have been implicated, after the onset of zygotic expression, in inducing and maintaining ventro-lateral cell fate during early development. We provide evidence here that a maternally expressed bone morphogenetic protein (BMP), Radar, may control early ventral specification in ze...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00470-6
更新日期：2000-12-01 00:00:00
abstract：:High expression of the B1 Sox genes, Sox2 and Sox3, is associated with the development of definitive neural primordia, the neural plates, in early stage embryos. However, in the caudal lateral epiblast (CLE) where axial stem cells reside, Sox2 and Sox3 are expressed at low levels, together with Brachyury. Because axia...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2014.01.003
更新日期：2014-05-01 00:00:00
abstract：:We have analysed the cell lineage relationships between larval and imaginal mesodermal primordia at the blastoderm stage by homotopic single cell transplantations. The primordia of adepithelial cells, the precursors of adult thoracic muscles, are restricted to the region from 50 to 65% egg length within the ventrally ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00654-0
更新日期：1997-02-01 00:00:00
abstract：:Splotch mice, which harbour mutations in the Pax3 gene, exhibit neural crest-related abnormalities including pigmentation defects, reduced or absent dorsal root ganglia and failure of cardiac outflow tract septation in homozygotes. Although splotch neural crest cells fail to colonise target tissues, they initiate migr...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00151-2
更新日期：1997-12-01 00:00:00
abstract：:In many animals, germline progenitors are kept undifferentiated to give rise to germline stem cells (GSCs), enabling continuous production of gametes throughout animal life. In the Drosophila ovary, GSCs arise from a subset of primordial germ cells (PGCs) that stay undifferentiated even after gametogenesis has started...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2013.01.002
更新日期：2013-04-01 00:00:00
abstract：:We performed a systematic screen for mutations affecting the trajectory of axons visualized by immunohistochemical staining of Medaka embryos with anti-acetylated tubulin antibody. Among the mutations identified, yanagi (yan) and kazura (kaz) mutations caused specific defects in projection of the posterior lateral lin...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2004.03.032
更新日期：2004-07-01 00:00:00
abstract：:We present the first time/space analysis using in situ hybridization for PTTG and prc1 genes during development of the mouse telencephalon. During the stages E11.5-E13.5 PTTG and prc1 are expressed in most tissues of the embryo. Within the telencephalon, PTTG and prc1 are found exclusively inside of the ventricular zo...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00243-4
更新日期：2000-04-01 00:00:00
abstract：:Syndecans are a family of heparan sulfate proteoglycans implicated in cell-cell and cell-matrix interactions. To investigate the roles of syndecans in early development, we identified three syndecan family members in Xenopus laevis: Xsyn-1, Xsyn-2, and Xsyn-3. Xsyn-1 and Xsyn-2 are maternal mRNAs localized to the anim...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(96)00584-9
更新日期：1996-10-01 00:00:00
