Abstract:
:C-terminal binding protein (CtBP) is an evolutionarily and functionally conserved transcriptional corepressor known to integrate diverse signals to regulate transcription. Drosophila CtBP (dCtBP) regulates tissue specification and segmentation during early embryogenesis. Here, we investigated the roles of dCtBP during development of the peripheral nervous system (PNS). Our study includes a detailed quantitative analysis of how altered dCtBP activity affects the formation of adult mechanosensory bristles. We found that dCtBP loss-of-function resulted in a series of phenotypes with the most prevalent being supernumerary bristles. These dCtBP phenotypes are more complex than those caused by Hairless, a known dCtBP-interacting factor that regulates bristle formation. The emergence of additional bristles correlated with the appearance of extra sensory organ precursor (SOP) cells in earlier stages, suggesting that dCtBP may directly or indirectly inhibit SOP cell fates. We also found that development of a subset of bristles was regulated by dCtBP associated with U-shaped through the PxDLS dCtBP-interacting motif. Furthermore, the double bristle with sockets phenotype induced by dCtBP mutations suggests the involvement of this corepressor in additional molecular pathways independent of both Hairless and U-shaped. We therefore propose that dCtBP is part of a gene circuitry that controls the patterning and differentiation of the fly PNS via multiple mechanisms.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Stern MD,Aihara H,Roccaro GA,Cheung L,Zhang H,Negeri D,Nibu Ydoi
10.1016/j.mod.2008.10.003subject
Has Abstractpub_date
2009-01-01 00:00:00pages
68-79issue
1-2eissn
0925-4773issn
1872-6356pii
S0925-4773(08)00141-Xjournal_volume
126pub_type
杂志文章abstract::pebble (pbl) is required for cytokinesis during postblastoderm mitoses (Hime, G., Saint, R., 1992. Zygotic expression of the pebble locus is required for cytokinesis during the postblastoderm mitoses of Drosophila. Development 114, 165-171; Lehner, C.F., 1992. The pebble gene is required for cytokinesis in Drosophila....
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00233-6
更新日期:2000-02-01 00:00:00
abstract::The embryonic fins and the tip of adult fins of teleost fish are supported by rows of straight, unmineralized fibrils called actinotrichia. The proximal ends of the actinotrichia are bundled and the mineralized bones called lepidotrichia are made along them. Since malformation in actinotrichia causes wavy fin bones, t...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2018.08.010
更新日期:2018-10-01 00:00:00
abstract::We have identified a short segment of the mouse Pax-6 gene 5' flanking region that is necessary and sufficient for reporter construct expression in components of the eye derived from non-neural ectoderm. This transcriptional control element has a highly conserved nucleotide sequence over 341 bp and is located approxim...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00057-4
更新日期:1998-05-01 00:00:00
abstract::Tubby is a mouse gene that may provide a model for adult-onset obesity in humans. It is a member of a four gene family in mammals that collectively encode the Tubby-like proteins (TULPs), putative transcription factors which share similar 260 amino acid 'tubby domains' at their C-termini. The mammalian genome also enc...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(02)00211-3
更新日期:2002-09-01 00:00:00
abstract::In the developing eye of Drosophila the protein kinase D-Raf controls the specification of the R7 photoreceptor cells. We show that overexpression of wild-type D-Raf inhibits the formation of R7 cells in a dose-dependent manner. Conversely, overexpression of mutant D-Raf proteins in which the conserved S388 is replace...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(97)00052-x
更新日期:1997-06-01 00:00:00
abstract::Cadherins are Ca(+2)-dependent cell surface proteins involved in the specification of the adhesive properties of cells. They are supposed to play a critical role in morphogenesis and pattern formation. In this paper we show that in the sea urchin embryo there are at least two different cadherins of relative molecular ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(93)90054-2
更新日期:1993-04-01 00:00:00
abstract::The gene crumbs (crb) of Drosophila encodes a transmembrane protein with 30 EGF-like and four laminin A G-domain-like repeats in its extracellular domain. Loss-of-function mutations lead to severe disorganization and degeneration of ectodermally derived embryonic epithelia. In embryos homozygous for crb8F105, an amorp...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(93)90066-7
更新日期:1993-12-01 00:00:00
abstract::By means of subtractive and differential hybridization techniques we have identified a novel murine gene (1A13) the expression of which is developmentally regulated in the mouse brain. Comparison of the nucleotide and predicted protein sequence revealed closest relationship of 1A13 to human CoREST, a transcriptional c...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00477-4
更新日期:2001-10-01 00:00:00
abstract::To analyse the possible roles of Drosophila tumour suppressor genes, 1(2)gl and 1(2)gd, in differentiation programmes of imaginal cells, we investigated their interactions with two segment polarity genes, viz., cubitus interruptus Dominant (ci-D) and engrailed (en), by examining their patterns of expression in tumouro...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(95)00367-3
更新日期:1995-06-01 00:00:00
abstract::We have generated a null allele of the mouse Msx1 homeobox gene by insertion of an nlacZ reporter gene into its homeobox. The sensitivity of beta-galactosidase detection permitted us to reveal novel aspects of Msx1 gene expression in heterozygous embryos, in particular in ectoderm and mesoderm during gastrulation, and...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(97)00065-8
更新日期:1997-07-01 00:00:00
abstract::ZT3, isolated from a murine muscle cell cDNA library by a low-stringency hybridization, encodes a zinc finger domain containing factor with a transcript of 5.0 kb. A 3' 2.5 kb partial nucleotide sequence contains an ORF of 1.5 kb where 17 canonical C2H2 zinc finger domains organized in tandem were identified. It maps ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(95)00465-3
更新日期:1996-01-01 00:00:00
abstract::To elucidate roles of fibroblast growth factors (FGF)18 during vertebrate development, we examined expression patterns of Fgf18 in chick embryos and observed effects of FGF18 protein on the Hensen's node, isthmus, and limb buds. Fgf18 is expressed on the right side of the node before the expression of Fgf8 starts. FGF...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00331-2
更新日期:2000-07-01 00:00:00
abstract::Polycomb group (PcG) proteins regulate and maintain expression pattern of genes set early during development. Although originally isolated as regulators of homeotic genes, PcG members play a key role in epigenetic mechanisms that maintain the expression state of a large number of genes. All members of the two polycomb...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2015.07.013
更新日期:2015-11-01 00:00:00
abstract::The spatial and temporal analysis of GATA-3 expression pattern in the human embryo revealed its expression in new anatomical sites. These include the endoderm of the primitive foregut, pharynx and allantois, the branchial arches and the mesenchymal cells surrounding the stomach and dorsal aorta. On the other hand, hum...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00088-x
更新日期:1999-07-01 00:00:00
abstract::The expression pattern of the receptor tyrosine kinase gene EphB3 was examined during the early stages of chick embryogenesis, and is described in this report. In the gastrula, EphB3 is expressed in epiblast cells adjacent to and entering the anterior portion of the primitive streak; expression is extinguished once ce...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00363-x
更新日期:2001-06-01 00:00:00
abstract::Broad (br), a transcription factor containing the Broad-Tramtrack-Bric-a-brac (BTB) and zinc finger domains was shown to mediate 20-hydroxyecdysone (20E) action and pupal development in Drosophila melanogaster and Manduca sexta. We determined the key roles of br during larval-pupal metamorphosis using RNA interference...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2007.11.001
更新日期:2008-03-01 00:00:00
abstract::Increases in Na/K-ATPase activity occur concurrently with the onset of cavitation and are associated with increases in Na(+)-pump subunit mRNA and protein expression. We have hypothesized that the alpha1-isozyme of the Na/K-ATPase is required to mediate blastocyst formation. We have tested this hypothesis by character...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2004.04.005
更新日期:2004-05-01 00:00:00
abstract::When an amphibian limb, cockroach leg or Drosophila imaginal disc is subjected to a surgical operation, it is capable of regenerating or duplicating certain parts. Although the structure of the regenerated tissue varies depending on the location and mass of the amputated or transplanted part, it can be predicted from ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(92)90043-j
更新日期:1992-12-01 00:00:00
abstract::SPOCK is a modular proteoglycan, with homology with proteins involved in cell adhesion processes and neurogenesis. We have previously shown that SPOCK transcripts predominate in the adult mouse brain. Here, we report its expression during mouse embryonic development by in situ hybridization, and immunocytochemistry. S...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00255-5
更新日期:2000-02-01 00:00:00
abstract::During Drosophila eye development, cell differentiation is preceded by the formation of a morphogenetic furrow, which progresses across the epithelium from posterior to anterior. Cells within the morphogenetic furrow are apically constricted and shortened along their apical-basal axis. However, how these cell shape ch...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2008.04.005
更新日期:2008-08-01 00:00:00
abstract::Here we report the use of double-stranded RNA (dsRNA) and morpholino technologies to specifically 'knock down' gene expression in early postimplantation mouse embryos. Sequence specific interference mediated by either dsRNA or by morpholino has been a useful tool for studying gene function in several organisms. Howeve...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(02)00191-0
更新日期:2002-10-01 00:00:00
abstract::A gene transfer method to ectopically express genes during chicken limb pattern formation using replication defective retroviral vectors has been established. Spherical non-proliferating (mitomycin C treated) aggregates of clonal retrovirus producing cells were grafted directly into developing chicken wing buds. The c...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(93)90035-v
更新日期:1993-10-01 00:00:00
abstract::ADAM19 is a member of the meltrin subfamily of ADAM metalloproteases. In Xenopus, ADAM19 is present as a maternal transcript. Zygotic expression starts during gastrulation and is apparent in the dorsal blastopore lip. ADAM19 expression through neurulation and tailbud formation becomes enriched in dorsal structures suc...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2008.10.010
更新日期:2009-03-01 00:00:00
abstract::During early vertebrate development, a series of neuromeres divides the central nervous system from the forebrain to the spinal cord. Here we examine in more detail the expression of Wnt-3, a member of the Wnt gene family of secreted proteins, in the developing diencephalon, in comparison to the expression of the home...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(92)90042-i
更新日期:1992-12-01 00:00:00
abstract::ADAMs are multidomain cell surface proteins that function in receptor-ligand processing, cell adhesion and fusion, and signaling. SpADAM, a single copy sea urchin ADAM gene with a 3072 bp open reading frame, is expressed during embryonic and larval development. The deduced SpADAM protein is 1023 amino acids long and i...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(02)00179-x
更新日期:2002-09-01 00:00:00
abstract::dkk-1 has recently been identified as a secreted protein in Xenopus laevis which is sufficient and necessary to cause head induction by antagonizing Wnt signalling (Glinka et al., 1998, Nature 391, 357-362). Consistent with such a role dkk-1 is expressed in the Spemann organizer of the early frog gastrula. Later, expr...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00194-x
更新日期:1999-12-01 00:00:00
abstract::The maturation of T cells is an intricate process involving the interaction of developing thymocytes with discrete microenvironments within the thymus. Numerous studies have indicated that distinct thymic compartments provide signals required for each stage of thymocyte maturation. In this study we performed a compreh...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(03)00121-7
更新日期:2002-12-01 00:00:00
abstract::The selective sensitivity of cells to programmed cell death (PCD) depends on the positive and negative death-inducing signals that converge into the apoptotic pathway. In Drosophila, the midline glial (MG) cells undergo selective death during development. Here, we show that the long isoform of the RNA-binding protein ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2008.10.004
更新日期:2009-01-01 00:00:00
abstract::In bovine preimplantation development, female embryos progress at lower rates and originate smaller blastocysts than male counterparts. Although sex-specific gene expression patterns are reported, when and how sex dimorphism is established is not clear. Differences among female and male early development can be useful...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2015.12.001
更新日期:2016-02-01 00:00:00
abstract::The gene expression patterns of primordial germ cells (PGCs) and embryonic stem cells were analyzed by a modified serial analysis of gene expression. During the process, we cloned a novel gene, PGC7, which was preferentially expressed in PGCs. Immunohistochemical analysis revealed that PGC7 was specifically expressed ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(02)00002-3
更新日期:2002-04-01 00:00:00