Abstract:
:X-chromosome inactivation (XCI) is an epigenetic mechanism that occurs in the eutherian embryo development to equalize the dosage of X-linked genes between males and females. This event is regulated by various factors, and the genes located in the X-chromosome inactivation center (XIC), which is known to be an evolutionary conserved region, are associated with XCI; however, a number of studies regarding this epigenetic event and genomic region are primarily performed in mouse models despite its species-specific features. Thus, in this study, the porcine XIC was identified, and we analyzed the expression of XIC-linked genes in porcine preimplantation embryos. Comparative sequence analysis revealed that the porcine XIC is synteny with that of human and the non-coding RNAs were less conserved compared with the protein coding genes in the XIC. Among the XIC-linked genes, the expression levels of CHIC1 and RLIM were decreased from morula to blastocyst development and their dosage was compensated between the male and female blastocysts. Additionally, the CpG sites of CHIC1 were approximately 50% methylated in parthenote blastocysts. Contrary to these genes, XIST and LOC102165544, an uncharacterized non-coding gene, showed dramatically increased expression levels after the morula stage and preferential female expression in blastocysts. Imprinted XIST expression was not observed, and their CpG sites were hypo-methylated in parthenogenic blastocysts. These results demonstrate that the porcine XIC consists of an evolutionary conserved structure with fewer sequences conserved non-coding RNAs. In addition, a few XIC-linked genes would likely achieve dosage compensation, but XCI would not be completed in porcine blastocysts.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Hwang JY,Oh JN,Park CH,Lee DK,Lee CKdoi
10.1016/j.mod.2015.10.005subject
Has Abstractpub_date
2015-11-01 00:00:00pages
246-55eissn
0925-4773issn
1872-6356pii
S0925-4773(15)30034-4journal_volume
138 Pt 3pub_type
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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更新日期:2001-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2002-03-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2017-02-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章,评审
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
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更新日期:2000-05-01 00:00:00
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journal_title:Mechanisms of development
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