Abstract:
:dkk-1 has recently been identified as a secreted protein in Xenopus laevis which is sufficient and necessary to cause head induction by antagonizing Wnt signalling (Glinka et al., 1998, Nature 391, 357-362). Consistent with such a role dkk-1 is expressed in the Spemann organizer of the early frog gastrula. Later, expression can be observed in an endomesodermal domain corresponding to the prospective prechordal plate, in two longitudinal stripes flanking the anterior chordamesoderm and in the precursors of the liver. At late neurula stage expression occurs in the prechordal plate adjacent to the prospective forebrain and eyes and in a stripe corresponding to the forming somites. dkk-1 is part of a gene family with at least three family members which is conserved between species. Its mouse homologue, Dkk-1, is first expressed at embryonic day (E) 6.5 in mesodermal cells adjacent to the embryonic/extraembryonic junction. Starting at E7.5 transcripts can be detected in the head mesoderm and at E8.5 additionally in developing somites (Glinka et al., 1998, Nature 391, 357-362). In this study we focus on the highly dynamic pattern of Dkk-1 mRNA distribution during mouse limb development from E9.0-E14.5. The other currently known family members, Dkk-2 and -3, are not expressed in the limb bud before E11.5 (C. Niehrs, pers. commun.) while the limb pattern is established. We show that Dkk-1 expression starts with the first sign of forelimb budding, whereas in the presumptive hindlimb region transcription becomes already apparent before the limb starts to bud out. Expression then becomes confined to two mesenchymal domains at E10.5 and E11.5. Using double-whole mount in situ hybridization we show that the posterior Dkk-1 expression domain initially overlaps with that of Shh, one of the key signalling molecules in limb development. Later, the two expression domains become separated. At E12.5-E14.5 Dkk-1 transcripts are restricted to the interdigital mesenchyme.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Grotewold L,Theil T,Rüther Udoi
10.1016/s0925-4773(99)00194-xsubject
Has Abstractpub_date
1999-12-01 00:00:00pages
151-3issue
1-2eissn
0925-4773issn
1872-6356pii
S0925-4773(99)00194-Xjournal_volume
89pub_type
杂志文章abstract::During heart development, cells of the endocardial cushions undergo an epithelial-mesenchymal transformation and migrate into the surrounding extracellular matrix. This event is required for the normal formation of valves and chamber septation. Coincident with this phenotypic change is the expression of the serine pro...
journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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更新日期:1998-12-01 00:00:00
abstract::The study of distal lung morphogenesis and vascular development would be greatly facilitated by an in vitro or ex vivo experimental model. In this study we show that the growth of mouse embryonic day 12.5 lung rudiments implanted underneath the kidney capsules of syngeneic or immunodeficient hosts follows closely lung...
journal_title:Mechanisms of development
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abstract::An important question in oocyte-mediated nuclear reprogramming is whether gene expression of the donor nucleus changes randomly or follows a pattern. Since cloned embryos are very heterogeneous and arrest frequently during preimplantation development, a random scenario is generally accepted. In the present study, we r...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2007.09.007
更新日期:2008-01-01 00:00:00
abstract::Msg1 and Mrg1 are founding members of a gene family which exhibit distinct patterns of gene expression during mouse embryogenesis. Sequence analysis reveals that these genes are unlike any other gene identified to date, but they share two near-identical sequence domains. The Msg1 and Mrg1 expression profiles during ea...
journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:1998-03-01 00:00:00
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journal_title:Mechanisms of development
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doi:10.1016/s0925-4773(99)00342-1
更新日期:2000-04-01 00:00:00
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journal_title:Mechanisms of development
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更新日期:1994-02-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/j.mod.2007.06.004
更新日期:2007-08-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00243-3
更新日期:1999-03-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00347-6
更新日期:2000-08-01 00:00:00
abstract::The putative vertebrate morphogen retinoic acid (RA) has been shown to induce expression of many mammalian homeobox genes in cell lines, suggesting expression of this gene family in developing vertebrate embryos may be controlled in part by RA. Using the teratocarcinoma cell line F9 as a model system, we have studied ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(92)90055-o
更新日期:1992-09-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00167-1
更新日期:1998-11-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2008.10.003
更新日期:2009-01-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00079-3
更新日期:1998-07-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
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更新日期:2002-05-01 00:00:00
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journal_title:Mechanisms of development
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更新日期:1997-12-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2004.03.016
更新日期:2004-07-01 00:00:00
abstract::To understand the mechanisms regulating the tissue non-specific alkaline phosphatase (TNAP) activity during development, we characterized cis-transcriptional regulatory elements. In embryonic cells and tissues, TNAP expression was driven preferentially by the exon 1A (E1A) promoter, one of the two promoters previously...
journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00566-4
更新日期:2002-01-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/j.mod.2003.06.004
更新日期:2003-11-01 00:00:00
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journal_title:Mechanisms of development
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更新日期:2019-06-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:1991-06-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2003.12.001
更新日期:2004-02-01 00:00:00
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journal_title:Mechanisms of development
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更新日期:2000-08-01 00:00:00