Abstract：
:SIP1 encodes a DNA-binding transcription factor that regulates multiple developmental processes, as highlighted by the pleiotropic defects observed in Mowat-Wilson syndrome, which results from mutations in this gene. Further, in adults, dysregulated SIP1 expression has been implicated in both cancer and fibrotic diseases, where it functionally links TGFβ signaling to the loss of epithelial cell characteristics and gene expression. In the ocular lens, an epithelial tissue important for vision, Sip1 is co-expressed with epithelial markers, such as E-cadherin, and is required for the complete separation of the lens vesicle from the head ectoderm during early ocular morphogenesis. However, the function of Sip1 after early lens morphogenesis is still unknown. Here, we conditionally deleted Sip1 from the developing mouse lens shortly after lens vesicle closure, leading to defects in coordinated fiber cell tip migration, defective suture formation, and cataract. Interestingly, RNA-Sequencing analysis on Sip1 knockout lenses identified 190 differentially expressed genes, all of which are distinct from previously described Sip1 target genes. Furthermore, 34% of the genes with increased expression in the Sip1 knockout lenses are normally downregulated as the lens transitions from the lens vesicle to early lens, while 49% of the genes with decreased expression in the Sip1 knockout lenses are normally upregulated during early lens development. Overall, these data imply that Sip1 plays a major role in reprogramming the lens vesicle away from a surface ectoderm cell fate towards that necessary for the development of a transparent lens and demonstrate that Sip1 regulates distinctly different sets of genes in different cellular contexts.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Manthey AL,Lachke SA,FitzGerald PG,Mason RW,Scheiblin DA,McDonald JH,Duncan MKdoi
10.1016/j.mod.2013.09.005subject
Has Abstractpub_date
2014-02-01 00:00:00pages
86-110eissn
0925-4773issn
1872-6356pii
S0925-4773(13)00081-6journal_volume
131pub_type
杂志文章abstract：:The tetrapod vertebral column has become increasingly complex during evolution as an adaptation to a terrestrial life. At the same time, the evolution of the vertebral formula became subject to developmental constraints acting on the size of the cervical and thoraco-lumbar regions. In the course of our studies concern...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2015.07.006
更新日期：2015-11-01 00:00:00
abstract：:We describe the expression of three Runt-related RUNX genes (previously termed AML, Cbfa, or Pebp2alpha) Runx1 and Runx3 during the development of teeth and other craniofacial tissues and compare them to Runx2 expression reported earlier. All three genes were expressed in mesenchymal condensates. Runx1 was expressed i...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(03)00101-1
更新日期：2002-12-01 00:00:00
abstract：:The availability of the draft sequence of the chicken genome will undoubtedly propel an already important vertebrate research model, the domestic chicken, to a new level. This review describes aspects of chicken natural history and cross-disciplinary biological value. The diversity of extant genetic variants available...
journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/j.mod.2004.05.018
更新日期：2004-09-01 00:00:00
abstract：:Mammalian embryos develop in coordination with extraembryonic tissues, which support embryonic development by implanting embryos into the uterus, supplying nutrition, providing a confined niche, and also providing patterning signals to embryos. Here, we show that in mouse embryos, the expansion of the amniotic cavity ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2014.01.004
更新日期：2014-05-01 00:00:00
abstract：:Mutations in the CFTR gene cause cystic fibrosis (CF) with myocardial dysfunction. However, it remains unknown whether CF-related heart disease is a secondary effect of pulmonary disease, or an intrinsic primary defect in the heart. Here, we used zebrafish, which lack lung tissue, to investigate the role of CFTR in ca...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2020.103627
更新日期：2020-09-01 00:00:00
abstract：:The mouse and human Hox complex consists of 39 genes in four linkage groups (A-D). Although the structure and expression patterns of most of these genes have been reported, the 5' members of the Hox C linkage group have been only partially characterized. Herein we report the primary and genomic structure of the mouse ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00182-2
更新日期：1998-01-01 00:00:00
abstract：:Mll, Brg1 and Brm are vertebrate homologues of Drosophila trithorax group (trxG) genes. We isolated chicken Mll cDNA clones, and examined patterns of Mll, Brg1 and Brm expression in chick embryos. All three genes were expressed from embryonic stage 2 onwards. Mll transcripts were just detectable in all tissues by in s...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00207-x
更新日期：1999-01-01 00:00:00
abstract：:Msg1 and Mrg1 are founding members of a gene family which exhibit distinct patterns of gene expression during mouse embryogenesis. Sequence analysis reveals that these genes are unlike any other gene identified to date, but they share two near-identical sequence domains. The Msg1 and Mrg1 expression profiles during ea...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00011-2
更新日期：1998-03-01 00:00:00
abstract：:Maleless (mle) is essential in Drosophila melanogaster males both in somatic cells and in germ cells. In somatic cells mle is necessary for X-chromosome dosage compensation. The role of mle in the germline is unknown. We have analyzed the expression pattern and localization of MLE, the other MSLs and acetylated isofor...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00009-4
更新日期：1998-02-01 00:00:00
abstract：:Fetal liver is the major embryonic hematopoietic organ and is extrinsically colonized by circulating hematopoietic stem cells (HSCs). Integrin beta-1 expression on HSCs is crucial for colonization, suggesting that interaction of Integrin beta-1 with extra-cellular matrix (ECM) factors promotes HSC adherence to fetal l...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2012.09.003
更新日期：2013-02-01 00:00:00
abstract：:In this paper we analyze the expression in follicular cells and regulation of the vitelline membrane protein gene we identified in region 32E of the second chromosome of D. melanogaster (VMP32E). We report germ line transformation results obtained with different kinds of gene fusion leading to the identification of a ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(91)90018-2
更新日期：1991-11-01 00:00:00
abstract：:Dystroglycan is a transmembrane receptor protein that provides a structural linkage between extracellular matrix components and cytoskeletal proteins. It was originally characterized as a member of dystrophin associated protein complex in muscle but, unlike other proteins of this complex, mutations in the dystroglycan...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(03)00091-1
更新日期：2002-12-01 00:00:00
abstract：:The sex determining gene, Sry, determines the sex of the organism by initiating development of a testis rather than an ovary from the cells of the bipotential gonad. In the 10 years since the discovery of Sry, new genes and cellular pathways that operate in the establishment of the gonadal primordium and the initiatio...
journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/s0925-4773(99)00327-5
更新日期：2000-03-15 00:00:00
abstract：:Mutations in the renin-angiotensin system (RAS) genes are associated with congenital anomalies of the kidney and urinary tract (CAKUT). As angiotensin (Ang) II, the principal effector peptide growth factor of the RAS, stimulates ureteric bud (UB) branching in whole intact embryonic (E) metanephroi, defects in UB morph...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2011.07.002
更新日期：2011-09-01 00:00:00
abstract：:We have identified ramshackle (ram) as a dominant suppressor of hedgehog loss-of-function in the developing Drosophila eye. We have characterized the gene and it encodes a double bromodomain protein with eight WD40 repeats. The Ram protein is localized predominantly to polytene chromosome interbands and is required fo...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2006.06.007
更新日期：2006-08-01 00:00:00
abstract：:DCC (deleted in colon cancer), Neogenin and UNC-5 are all members of the immunoglobulin superfamily of transmembrane receptors which are believed to play a role in axon guidance by binding to their ligands, the Netrin/UNC-40 family of secreted molecules (Cell. Mol. Life Sci. 56 (1999) 62; Curr. Opin. Genet. Dev. 7 (19...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00513-5
更新日期：2001-11-01 00:00:00
abstract：:The recent determination and annotation of the entire euchromatic sequence of the Drosophila melanogaster genome predicted the existence of about 13600 different genes (Science 287 (2000) 2185; http://www.fruitfly.org/annot/index.html). In parallel, the Berkeley Drosophila Genome Project (BDGP) has undertaken systemat...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00566-4
更新日期：2002-01-01 00:00:00
abstract：:Cadherins are Ca(+2)-dependent cell surface proteins involved in the specification of the adhesive properties of cells. They are supposed to play a critical role in morphogenesis and pattern formation. In this paper we show that in the sea urchin embryo there are at least two different cadherins of relative molecular ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(93)90054-2
更新日期：1993-04-01 00:00:00
abstract：:The human RUNX3/AML2 gene belongs to the 'runt domain' family of transcription factors that act as gene expression regulators in major developmental pathways. Here, we describe the expression pattern of Runx3 during mouse embryogenesis compared to the expression pattern of Runx1. E10.5 and E14.5-E16.5 embryos were ana...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00537-8
更新日期：2001-12-01 00:00:00
abstract：:The Drosophila homeobox gene engrailed (en) is needed for correct embryonic development, and related sequences are active during vertebrate embryogenesis. Here we report the protein coding sequence and embryonic expression pattern of the zebrafish engrailed-2 gene (eng-2) which is directly homologous to En-2 in mice a...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(92)90025-f
更新日期：1992-11-01 00:00:00
abstract：:The homeotic or Hox genes encode a network of conserved transcription factors which provide axial positional information and control segment morphology in development and evolution. During embryonic brain development of Drosophila, the Hox gene labial (lab) is essential for tritocerebral neuromere specification; lab l...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2005.09.004
更新日期：2006-01-01 00:00:00
abstract：:ADAMs are multidomain cell surface proteins that function in receptor-ligand processing, cell adhesion and fusion, and signaling. SpADAM, a single copy sea urchin ADAM gene with a 3072 bp open reading frame, is expressed during embryonic and larval development. The deduced SpADAM protein is 1023 amino acids long and i...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(02)00179-x
更新日期：2002-09-01 00:00:00
abstract：:Using the epidermis-specific cytokeratin 14 promoter to deliver HGF exclusively from epidermal keratinocytes, we have examined the potential of hepatocyte growth factor (HGF) secreted from the normal environment to control morphogenesis. The transgenic mice displayed a significant increase of the number of melanocytes...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(00)00308-7
更新日期：2000-06-01 00:00:00
abstract：:The diencephalon is the caudal part of the developing forebrain that gives rise to the epithalamus, thalamus, hypothalamus and retina. The mature diencephalon is functionally and anatomically parceled into well-defined nuclei. In an effort to understand how this region of the brain develops we examined the expression ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(02)00091-6
更新日期：2002-07-01 00:00:00
abstract：:The recessive mutant mouse jumonji (jmj), obtained by a gene trap strategy, shows neural tube defects in approximately half of homozygotes with a Balb/cA and 129/Ola mixed background. Here, we show that no neural tube defects are observed with a Balb/cA background. We also found hypoplasia of the liver, thymus and spl...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(97)00082-8
更新日期：1997-08-01 00:00:00
abstract：:Homeodomain transcription factor CnNK-2 seems to play a major role in foot formation in Hydra. Recently, we reported in vitro evidence indicating that CnNK-2 has autoregulatory features and regulates expression of the morphogenetic peptide pedibin. We proposed that CnNK-2 and pedibin synergistically orchestrate foot d...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2005.04.010
更新日期：2005-09-01 00:00:00
abstract：:Egr1 is a highly conserved zinc finger protein which plays important roles in many aspects of vertebrate development and in the adult. The cDNA coding for zebrafish Egr1 was obtained and its expression pattern was examined during zebrafish embryogenesis using whole-mount in situ hybridization. Egr1 mRNA is first detec...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(02)00283-6
更新日期：2002-10-01 00:00:00
abstract：:The chick dermis is known to control the formation of feathers and interfeathery skin in a hexagonal pattern. The evidence that the segregation of two types of fibroblasts involves Delta/Notch signalling is based on three facts. Rings of C-Delta-1-expressing fibroblasts precede and delimit the forming feather primordi...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00027-6
更新日期：1998-03-01 00:00:00
abstract：:Directional migration of primordial germ cells (PGCs) toward future gonads is a common feature in many animals. In zebrafish, mouse and chicken, SDF-1/CXCR4 chemokine signaling has been shown to have an important role in PGC migration. In Xenopus, SDF-1 is expressed in several regions in embryos including dorsal mesod...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2009.09.005
更新日期：2010-01-01 00:00:00
abstract：:In the Drosophila embryo, a subset of muscles require expression and function of the RYK subfamily RTK gene derailed (drl) for correct attachment. We have isolated a second RYK homolog, doughnut (dnt), from Drosophila. The DNT protein exhibits 60% amino acid identity to DRL, and is structurally as similar to the mamma...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(98)00167-1
更新日期：1998-11-01 00:00:00
