Abstract:
:Following tissue damage the immune response, including inflammation, has been considered an inevitable condition to build the host defense against invading pathogens. The recruitment of innate immune leukocytes to injured tissue is observed in both vertebrates and invertebrates. However, it is still not conclusive whether the inflammatory response is also indispensable for the wound healing process by itself, in addition to its role in microbial clearance. In this study we determine the requirement of innate immune cells, both hemocytes and fat body cells, in Drosophila imaginal disc regeneration. We investigate wound healing and regenerative cell proliferation of damaged imaginal discs under immunodeficient conditions. To delay development of Drosophila at matured third instar larval stage we used a sterol-mutant erg2 knock-out yeast strain in the medium. This dietary-controlled developmental arrest allowed us to generate larvae free of immune cells without interfering with their larval development. In addition, this approach allowed uncoupling regenerative cell proliferation of damaged discs from their normal developmental growth. We furthermore examined the regenerative cell proliferation of fragmented imaginal discs by transplantation into host flies deficient of immune cells. We demonstrate that the damaged/fragmented discs in immune cells deficient conditions still exhibit regenerative cell proliferation comparable to those of control samples. These results suggest that recruitment of immune cells is not a prerequisite for the regenerative growth of damaged imaginal discs.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Katsuyama T,Paro Rdoi
10.1016/j.mod.2012.11.005subject
Has Abstractpub_date
2013-02-01 00:00:00pages
112-21issue
2-3eissn
0925-4773issn
1872-6356pii
S0925-4773(12)00116-5journal_volume
130pub_type
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/j.mod.2012.11.001
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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pub_type: 杂志文章
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更新日期:1992-03-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章,评审
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