Abstract:
:A reversed-phase high-performance liquid chromatographic assay for the determination of the HIV protease inhibitors amprenavir (Agenerase) and indinavir (Crixivan) in human plasma is described, using a mobile phase consisting of 0.50 M phosphate buffer (adjusted to pH 5,5) - Milli-Q water - acetonitrile (120: 1,080: 800, v/v/v). A solid-phase extraction using C18 extraction columns (Discovery columns 100 mg, 1 ml Supelco) and a liquid-liquid extraction with 0.5 ml hydrogenocarbonate/carbonate buffer (adjusted to pH 10.6) and 6 ml methyl ter-butyl ether have been compared. The liquid-liquid extraction has been chosen to be easier and cheaper. The method has been validated over the range of 60 to 3,000 ng/ml for amprenavir and 20 to 3,000 ng/ml for indinavir using a 0.5 ml sample volume. The specificity, linearity, accuracy and precision have been studied. The limit of detection was respectively for amprenavir and indinavir 15 and 4 ng, and the limit of quantification was 60 and 20 ng/ml. Stability tests under various conditions were performed. This assay can readily be used in a hospital laboratory for the routine monitoring of plasma concentrations of amprenavir in HIV-infected patients. The trough plasma concentrations average has been determined in patients treated by amprenavir and indinavir for seven months.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Laussine S,Roux A,Delhotal-Landes B,Flouvat Bsubject
Has Abstractpub_date
2001-03-01 00:00:00pages
169-75issue
2eissn
0003-3898issn
1950-6112journal_volume
59pub_type
杂志文章abstract::We have evaluated the methodological quality of the Rémic (microbiology guidelines - bacteriology and mycology) of the Société française de microbiologie (edition2007), using to AGREE criteria, which are consensual at an international level, in particular at the the World Health Organisation (WHO) and at the European ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2011.0536
更新日期:2011-03-01 00:00:00
abstract::Guanidinoacetate (GAA) and creatine (Cr) are creatine deficiency syndromes (CDS) biochemical markers. We describe a liquid chromatography - tandem mass spectrometry method (LC/MSMS) performing simultaneous analysis of GAA, Cr and creatinine (Crn). Study of Cr uptake by fibroblasts for Cr transporter defect diagnosis i...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2013.0871
更新日期:2013-07-01 00:00:00
abstract::We present the distribution of cytochrome P450, the terminal oxidase of the microsomal drug-metabolizing system among the different species, organs, and its subcellular localization. Physicochemical properties, and more specially spectral properties, of this hemoprotein are reviewed. We also described the two microsom...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1975-01-01 00:00:00
abstract::Three methods (rapid bioassay, enzyme immunoassay and fluoroimmunoassay) for the determination of amikacin level in serum are compared. The statistical analysis of the results show correlation coefficients equal to 0.967 (EMIT versus FIA), 0.970 (bioassay versus FIA) and 0.983 (bioassay versus EMIT). The three methods...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::Sixty-three strains of Pseudomonas paucimobilis, a Gram-negative, lemon yellow pigmented, glucose nonfermenting bacillus are phenotypically compared to the type strain of this new species (ATCC 29837). The morphological, cultural, biochemical (ONPG-test, oxidase, aesculin positive) and nutritional (assimilation of man...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Diagnosis of type III hyperlipoproteinemia requires preparative ultracentrifugation in order to measure the cholesterol/triglyceride molar ratio into isolated d less than 1.006 lipoproteins. The authors describe an ultracentrifugation micromethod which needs 600 microliter of serum and can be completed within three ho...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::The Maroteaux-Lamy disease, or mucopolysaccharidosis type VI is an inherited metabolic disorder severe and rare. It is caused by a deficiency of the enzyme arylsulfatase B. It is characterized by a heterogeneous clinical, radiological and genetic. We report the case of a Maroteaux-Lamy syndrome of in a child aged 7 ye...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2011.0627
更新日期:2011-11-01 00:00:00
abstract::Circulating citrulline is emerging as an innovating biomarker candidate for assessment of intestinal function. Amino acid synthesized by enterocytes of intestinal mucosal, citrulline is not included in proteins or nutrition products and is a precursor for production of arginine by the kidney. Plasma citrulline is, in ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:10.1684/abc.2011.0609
更新日期:2011-09-01 00:00:00
abstract::It is often reminded that if the ionized calcium is not measured, the interpretation of total calcemia should consider serum albumin. Two formulas are usually employed: ["Corrected" Ca (mmol/L) = Ca measured (mmol/L) + 0.020 or 0.025 (40 - albumin (g/L))]. This adjustment formula arises from works of Payne published i...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2009.0348
更新日期:2009-07-01 00:00:00
abstract::We evaluated a rapid and semi-quantitative C-Reactive Protein test on whole blood, the Actim CRP (Fumouze). Based on immuno-chromatography technology, this test ranked the blood sample in four groups: < 10 mg/L, 10-40 mg/L, 40-80 mg/L and > 80 mg/L. This evaluation finds an excellent repeatability, the absence of hook...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2005-09-01 00:00:00
abstract::In vitro fertilization (IVF) and embryo transfer (ET) appear to constitute a revolution in the reproductive sciences rather than merely a new technique in the treatment of sterility. Principle of IVF: IVF accomplishes in vitro the process than normally occurs in the oviduct between the ovulation of oocyte II and embry...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::The recent development of technics of specific fixation using radio isotope-labelled tracer elements permits the demonstration of various types of receptors at the level of the lymphocyte membrane. Thus a new route is open in the understanding of the biochemical mechanisms controlling the physiology of the lymphocyte ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1980-01-01 00:00:00
abstract::The recent development of specific and sensitive biochemical markers reflecting the overall rate of bone formation and bone resorption, has markedly improved the non invasive assessment of bone turnover in various metabolic bone diseases, especially for osteoporosis. The immunoassay of human osteocalcin recognizing th...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1999-03-01 00:00:00
abstract::There has been rapid progress in techniques for the detection of antinuclear antibodies. The methods most widely used at present are indirect immunofluorescence, Ouchterlony double immunodiffusion, passive haemagglutination and the Farr test. Antinuclear antibodies recognise several antigens. With a few exceptions, in...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1982-01-01 00:00:00
abstract::Therapeutic monitoring of patients participating in methadone maintenance programmes requires the qualitative or quantitative determination of methadone in urine before and during treatment. Generally immunoassay techniques are used for this determination, but more and more often measurement of plasma or serum levels ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::The development of enzymo-immunological technics and the synthesis of efficacious immuno-adsorbants has given rise to several immuno-enzymometric methods of which one, ELISA (enzyme-linked immunosorbent assay) has proved particularly practical and sensitive for the estimation of antibodies. ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1978-01-01 00:00:00
abstract::Albumin is the major circulating protein. It plays a fundamental role maintaining intra-vascular oncotic pressure and carrying many endogenous and exogenous substances. Variations of plasma albumin levels can be physiologic or pathologic and both qualitative and quantitative (more frequent) disorders are regrouped und...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:2005-03-01 00:00:00
abstract::Haemochromatosis is the most common genetic disease in individuals of Northern European origin. This disorder of iron metabolism, for which the molecular basis remains poorly understood, is characterized by an excessive absorption of dietary iron through the duodenal mucosa. Progressive iron loading of parenchymal org...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1997-05-01 00:00:00
abstract::Anemia is the most common pathology encountered in hematology. Etiologies are numerous, so it is important to adopt a rigorous approach. Complementary examinations must be specific to the clinical situation in order to determine the mechanisms on the one hand and decide the therapeutic management on the other. We repo...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2011.0584
更新日期:2011-05-01 00:00:00
abstract::The diagnosis of double hit lymphoma remains a challenge for the biologist for a good management of the patient. This new category of lymphoma "double hit" (DH) is part of a new entity of the WHO classification 2008: « Unclassifiable B lymphoma with features intermediate between diffuse large cell B lymphoma and Burki...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2016.1189
更新日期:2016-12-01 00:00:00
abstract::A 58-year-old patient, without any notable medical history, except for alcoholism and treated hypertension, developed anemia and leukopenia with macrocytosis. Folate deficiency was diagnosed and subsequently treated. Despite folate supplementation, the hematological parameters did not normalize. Further diagnosis inve...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2013.0863
更新日期:2013-07-01 00:00:00
abstract::According to the French legislation on medical biology (January 16th, 2010), all biological laboratories must be accredited according to ISO 15189 for at least 50% of their activities before the end of 2016. The extraction of DNA from a sample of interest, whether solid or liquid is one of the critical steps in molecu...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2013.0891
更新日期:2013-09-01 00:00:00
abstract::Diagnosis of haematological malignancies is based on multiparametric analysis such as morphology, phenotype and genotype studies. Some entities are only defined by one of these approach. Flow-cytometry (FCM) is useful to determined the normal counterpart of the tumoral process and its differentiation status within the...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:2002-11-01 00:00:00
abstract::The diagnosis of Protein C (PC) congenital deficiency is of first importance because it leads, more frequently than Antithrombin III deficiency, to serious thromboembolic accidents in young patients, even when PC levels are slightly decreased (40 p. cent up to 60 p. cent). In order to measure PC activity, the authors ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Various coagulation abnormalities were reported in HIV-infected patients. Cases of severe thrombocytopenia were first observed in contaminated homosexual males, as well as prolonged APTT due to the presence of lupus-like anticoagulant with a frequency in the range 8 to 70% of the studied patients. Even if lupus antico...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1998-03-01 00:00:00
abstract::In assisted reproductive technology (ART), serum levels of several key hormones are used to evaluate ovarian follicular reserve and to monitor gonadotropin-stimulated follicle growth. Currently, serum estradiol, FSH, LH and inhibin B levels are examined and combined at the beginning of the menstrual cycle to evaluate ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:2003-09-01 00:00:00
abstract::Gas-liquid chromatography has been used directly on clinical specimens as a method for presumptive diagnosis of anaerobic infections. This technique proved to be valuable and reliable. Combined with a Gram stain, it offers the possibility to differentiate in less than 1 hour aerobic from anaerobic infections by means ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::The in vitro activity of the broad-spectrum antifungals miconazole, ketoconazole and itraconazole was evaluated by the decimal dilution method in liquid media, for respectively 2511, 1536 and 1859 strains of yeasts belonging to 31 species. Sabouraud broth was used for miconazole and brain heart infusion broth for keto...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Strongyloïdes stercoralis infection is a polymorphic and non specific clinical presentation. Often asymptomatic, it can be not seen. However, in patients with immunodeficiency, high parasite load can be observed, consequence of self-infestation cycle, and can spread throughout the body. This presentation of malignant ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2013.0809
更新日期:2013-03-01 00:00:00
abstract::The neuropathies associated with monoclonal IgM gammopathy reacted with glycoconjugated targets on a very antigenic epitope on the sulfated glucuronic glycolipids corresponding to SGPG and SGLPG (sulfoglucuronyl paragloboside and sulfoglucuronyl lactosaminyl paragloboside), myelin-associated glycoprotein (MAG) and sul...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00