Antigens of monoclonal antibody NB3C4 are novel markers for oligodendrocytes.

Abstract:

:We produced NB3C4, a novel monoclonal antibody specific for oligodendrocytes, using human neuroblastoma IMR-32 cells. NB3C4 specifically recognized oligodendrocytes in the CNS, although it bound to neuroblastoma IMR-32 cells and oligodendrocytes in vitro. Double immunofluorescence staining of rat brain using NB3C4 and anti-GST-pi, anti-glial fibrillary acidic protein (GFAP), or anti-neurofilament 200 (NF) antibody revealed that anti-GST-pi antibody identified an oligodendrocyte marker recognizing NB3C4-positive cells, while both anti-GFAP and anti-NF antibody did not. Western blotting of rat brain homogenates showed that NB3C4 bound three proteins of 22-28 kDa, while the anti-GST-pi recognized a 27 kDa protein. Therefore, antigens recognized by NB3C4 could be novel markers for oligodendrocytes.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Yoshimura K,Kametani F,Shimoda Y,Fujimaki K,Sakurai Y,Kitamura K,Asou H,Nomura M

doi

10.1097/00001756-200102120-00045

subject

Has Abstract

pub_date

2001-02-12 00:00:00

pages

417-21

issue

2

eissn

0959-4965

issn

1473-558X

journal_volume

12

pub_type

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