Abstract:
:Pulmonary tissue injury and repair processes involve complex and coordinated cellular events such as necrosis, inflammation, cell growth/differentiation, apoptosis, and remodeling of extracellular matrix. These processes are regulated by expression of multiple mediator genes. Commercially available microarray blots and slides allow screening of hundreds to thousands of genes in a given tissue or cell preparation. However, often these blots do not contain cDNAs of one's interest and are difficult to interpret. In order to analyze the tissue expression profile of a large number of genes involved in pulmonary injury and pathology, we developed a rat gene array filter using array technology. This array consisted of 27 genes representing inflammatory and anti-inflammatory cytokines, growth factors, adhesion molecules, stress proteins, transcription factors and antioxidant enzymes; 3 negative controls, and 2 blank spots. Using rat gene-specific polymerase chain reaction (PCR) primer pairs, cDNAs for these genes were amplified and cloned into a TA vector. Plasmids with recombinant cDNA inserts were purified and blotted onto a nylon membrane. Lung total RNA was isolated at 3 or 24 h following intratracheal (IT) exposure of male Sprague Dawley rats to either saline (control), residual oil fly ash (ROFA; 3.3 mg/kg) or metals found in one instillate of ROFA: nickel (NiSO(4); 1. 3 micromol/kg) or vanadium (VSO(4); 2.2 micromol/kg). (32)P-Labeled cDNA was generated from RNA samples in a reverse transcriptase reaction and subsequently hybridized to array blots. Densitometric scans of array blots revealed a twofold induction of interleukin (IL)-6 and TIMP-1 at 24 h post ROFA or Ni exposure. The pulmonary expressions of cellular fibronectin (cFn-EIIIA), ICAM-1, IL-1beta, and iNOS genes were also increased 24 h post ROFA-, V-, or Ni-exposure. Consistent hybridization of beta-actin in all array blots and absence of hybridization signals in negative controls indicated gene specific hybridization. ROFA or metal-induced increase in the expression of IL-6 observed in array blot was validated by Northern blot hybridization. Developing a pulmonary rat gene array may provide a tool for screening the expression profile of tissue specific markers following exposure to toxic air contaminants.
journal_name
Inhal Toxicoljournal_title
Inhalation toxicologyauthors
Nadadur SS,Schladweiler MC,Kodavanti UPdoi
10.1080/08958370050198566subject
Has Abstractpub_date
2000-12-01 00:00:00pages
1239-54issue
12eissn
0895-8378issn
1091-7691journal_volume
12pub_type
杂志文章abstract::Epidemiologic studies have provided strong evidence that episodic exposure to ambient particulate matter is associated with increases in morbidity and mortality. These adverse effects have been demonstrated at concentrations far below the National Ambient Air Quality Standard (NAAQS), and thus, the biological plausibi...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/089583799197276
更新日期:1999-01-01 00:00:00
abstract:CONTEXT:Air pollution exposure affects autonomic function, heart rate, blood pressure and left ventricular function. While the mechanism for these effects is uncertain, several studies have reported that air pollution exposure modifies activity of the carotid body, the major organ that senses changes in arterial oxygen...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.3109/08958378.2014.984881
更新日期:2015-01-01 00:00:00
abstract::This study aims to investigate the negative effects of chronic exposure to isoflurane on spermatogenesis and explore the underlying mechanisms. Sixty male rats were randomly allocated to two groups: control group, receiving no treatment, and anesthesia group, administrated exposure to isoflurane (2 ppm) for 25 consecu...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.3109/08958378.2015.1080772
更新日期:2015-01-01 00:00:00
abstract::Chronic exposure to chlorine gas has been shown to cause occupational asthma. Acute inhalation of chlorine is known to cause airway inflammation and induce airway nitric oxide formation. Exhaled nitric oxide may therefore be a marker of airway damage after chlorine gas exposure. After accidental chlorine gas exposure ...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958370701432157
更新日期:2007-08-01 00:00:00
abstract::Regulatory guidelines are intended to provide recommendations on ways to achieve greater harmonization in the interpretation and application of technical procedures and requirements for product registration in order to reduce or obviate replication of the testing carried out during the research and development of new ...
journal_title:Inhalation toxicology
pub_type: 杂志文章,评审
doi:10.3109/08958378.2012.757400
更新日期:2013-02-01 00:00:00
abstract:INTRODUCTION:Air pollution causes respiratory symptoms and pulmonary disease. Airway inflammation may be involved in the mechanism also for cardiovascular disease. Wood smoke is a significant contributor to air pollution, with complex and varying composition. We examined airway effects of two kinds of wood smoke in a c...
journal_title:Inhalation toxicology
pub_type: 临床试验,杂志文章
doi:10.3109/08958378.2011.633281
更新日期:2012-01-01 00:00:00
abstract::Oxidative stress is thought to be the pathogenesis of pulmonary fibrosis induced by asbestos, and heme oxygenase-1 (HO-1) protects lung tissue against oxidative stress. We hypothesized that HO-1 is associated with oxidative lung injury caused by exposure to asbestos. This study was conducted to investigate the time co...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958370590922580
更新日期:2005-05-01 00:00:00
abstract::Background: High-mobility group box 1 (HMGB1) protein plays an important pathogenic role in various diseases such as pulmonary fibrosis. However, the relationship between variation of HMGB1 gene and susceptibility to coal worker's pneumoconiosis (CWP) remains unclear. The objective of the study was to determine the as...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958378.2020.1764153
更新日期:2020-03-01 00:00:00
abstract::This study assessed the association between ambient air pollution and daily elementary school absenteeism in Washoe County, NV, between 1996 and 1998. All 57 elementary schools in Washoe County in northern Nevada were included in the data set. There was a total of 27,793 student enrollments during this study period. T...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958370050164626
更新日期:2000-11-01 00:00:00
abstract::Antisense oligonucleotides (ASOs) bind and facilitate degradation of RNA and inhibit protein expression in pathways not easily targeted with small molecules or antibodies. Interleukin (IL)-4 and IL-13 potentiate signaling through the shared IL-4 receptor-α (IL-4Rα) subunit of their receptors. ASO targeting of IL-4Rα m...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.3109/08958378.2014.907587
更新日期:2014-07-01 00:00:00
abstract::Acute lung injury (ALI) induced by phosgene increases risk of serious edema and mortality. Increased permeability of the microvascular endothelium is implicated in the progression of ALI, but the processing interaction and time course activity of the vascular regulators in exudation are still not understood. The main ...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958370802015091
更新日期:2008-07-01 00:00:00
abstract::The aim of this study was to investigate the potential cytotoxicity of solid lipid nanoparticles (SLN) loaded with sildenafil. The SLNs were tested as a new drug delivery system (DDS) for the inhalable treatment of pulmonary hypertension in human lungs. Solubility of sildenafil in SLN lipid matrix (30:70 phospholipid:...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.3109/08958378.2013.810315
更新日期:2013-08-01 00:00:00
abstract::We used inert melamin particles that are very well defined in size (diameters: 0.5, 1.9, 6.8 microm) to study the effects of ambient particles of <10 microm (PM(10)) on phagocytosis. Dose-response functions were found between the amount of added melamin particle mass and the toxicity. Fine particles (0.5 microm) were ...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958370252871005
更新日期:2002-04-01 00:00:00
abstract::The purpose of this work is to define a sample preparation protocol that allows inorganic fibers and particulate matter extracted from different biological samples to be characterized morphologically, crystallographically and chemically by transmission electron microscopy-energy dispersive spectroscopy (TEM-EDS). The ...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958378.2016.1175527
更新日期:2016-07-01 00:00:00
abstract::In 1999 Berry published a model for mesothelioma incidence following fiber exposure. He concluded, that the influence of the solubility of fibers on the mesothelioma rate is 17 times higher in humans than in rats. This conclusion may be helpful for evaluating the carcinogenic risk from man-made vitreous fibers, but it...
journal_title:Inhalation toxicology
pub_type: 杂志文章,评审
doi:10.1080/08958370490505016
更新日期:2004-10-01 00:00:00
abstract::Objectives: This follow-up study set out to evaluate the natural course and radiographic progression of silicosis among ceramic workers and describe the risk factors related with disease progression. Materials and methods: We retrospectively analyzed the data of ceramic workers with silicosis who were referred to our ...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958378.2019.1613459
更新日期:2019-02-01 00:00:00
abstract::Nasal efficiency for removing fine particles from inhaled air may be affected by variations in nasal structure associated with race. In 11 African American and 11 Caucasian adults (age 18-31 yr) we measured the fractional deposition (DF) of fine particles (1 and 2 mum mass median aerodynamic diameter) (MMAD) for oral ...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958370500188984
更新日期:2005-11-01 00:00:00
abstract::Epidemiologic studies of air pollution require accurate exposure assessments at unmonitored locations in order to minimize exposure misclassification. One approach gaining considerable interest is the land-use regression (LUR) model. Generally, the LUR model has been utilized to characterize air pollution exposure and...
journal_title:Inhalation toxicology
pub_type: 杂志文章,评审
doi:10.1080/08958370701495998
更新日期:2007-01-01 00:00:00
abstract:CONTEXT:Heart rate alterations associated with exposure to particulate matter (PM) and gaseous pollutants have been observed in epidemiological studies and animal experiments. Nevertheless, the time-lag of these associations is still unclear. OBJECTIVE:Determine the association at different time-lags between the compl...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.3109/08958370903349365
更新日期:2010-03-01 00:00:00
abstract::Increased lung cancer incidence with workers at the production site of crude silicon carbide (SiC) using the Acheson process has been reported. Several agents derived from the process were discussed as causative factors. Recently concern had been expressed about the presence of cleavage fragments (CFs) in commercial p...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.3109/08958378.2014.885099
更新日期:2014-04-01 00:00:00
abstract::Biodiesel made from the transesterification of plant- and animal-derived oils is an important alternative fuel source for diesel engines. Although numerous studies have reported health effects associated with petroleum diesel emissions, information on biodiesel emissions are more limited. To this end, a program at the...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.3109/08958378.2015.1076910
更新日期:2015-01-01 00:00:00
abstract::This study was designed to investigate effects of sulfur dioxide (SO(2)) and its derivatives (bisulfite and sulfite) on the rat blood pressure. The blood pressures of male Wistar rats exposed to SO(2) and its derivatives at various doses were measured. Findings were that: (1) with acute-one time exposure to SO(2) for ...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958370390215785
更新日期:2003-08-01 00:00:00
abstract::The C9 alkylbenzenes, composed mostly of ethyltoluenes and trimethylbenzenes, comprise 75-90% of the naphtha fraction of crude oil. Occupational and environmental exposure to C9 alkylbenzenes occur via inhalation. We conducted short-term inhalation studies on the ethyltoluene isomers (2-, 3- or 4-) to select one isome...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958378.2018.1430190
更新日期:2017-01-01 00:00:00
abstract::Epidemiology studies of populations living in areas with good air quality report correlations between levels of ambient particulate matter (PM) and mortality rates. These associations occur at low PM concentrations that are below current air quality standards. Can such concentrations cause mortality, given the toxicit...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958370490442935
更新日期:2004-01-01 00:00:00
abstract::Laboratory animals exposed to methyl iodide (MeI) have previously demonstrated lesions of the olfactory epithelium that were associated with local metabolism in the nasal tissues. Interactions of MeI in the nasal passage may, therefore, alter systemic toxicokinetics. The current study used unrestrained plethysmographs...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958370802596926
更新日期:2009-05-01 00:00:00
abstract::NASA is planning to build a habitat on the Moon and use the Moon as a stepping stone to Mars. JSC-1, an Arizona volcanic ash that has mineral properties similar to those of lunar soil, is used to produce lunar environments for instrument and equipment testing. NASA is concerned about potential health risks to workers ...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.1080/08958370701821219
更新日期:2008-01-01 00:00:00
abstract:CONTEXT:Sulfur mustard exposure, as the most widely used chemical weapon, can lead to acute and long-term pulmonary complications via various pathways, such as triggering an imbalance between the oxidant and antioxidant system. Currently, there is no validated antidote, chemoprophylaxis and curative modality for pulmon...
journal_title:Inhalation toxicology
pub_type: 杂志文章,评审
doi:10.3109/08958378.2014.920439
更新日期:2014-08-01 00:00:00
abstract:CONTEXT:There have been no animal studies of the health effects of repeated inhalation of mixtures representing downwind pollution from coal combustion. Environmental exposures typically follow atmospheric processing and mixing with pollutants from other sources. OBJECTIVE:This was the fourth study by the National Env...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.3109/08958378.2011.572932
更新日期:2011-05-01 00:00:00
abstract::Continuous environmental or occupational exposure to airborne particulate pollution is believed to be a major hazard for human health. A technique to characterize their deposition and clearance from the lungs is fundamental to understand the underlying mechanisms behind their negative health effects. In this work, we ...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.3109/08958378.2010.549856
更新日期:2011-02-01 00:00:00
abstract::Cigarette smoke exposures in mice have been conducted under various exposure conditions using different strains as animal models of smoke-related diseases. We exposed cigarette smoke to two strains of mice [C57BL/6J (C57) and AKR/J (AKR)] under two different exposure regimens (1 h or 4 h/day) at equivalent daily expos...
journal_title:Inhalation toxicology
pub_type: 杂志文章
doi:10.3109/08958378.2011.596851
更新日期:2011-08-01 00:00:00