Abstract：
:We investigated the influence of muramyl dipeptide (MDP), a cell wall component of Gram-positive bacteria, on cytokine-induced nitric oxide (NO) production in rat primary astrocytes. MDP alone did not induce NO release in astrocyte cultures. However, MDP increased astrocyte NO production and subsequent nitrite accumulation triggered by IFN-gamma. IFN-gamma-activated expression of mRNA for inducible NO synthase (iNOS) and iNOS transcription factor interferon regulatory factor-1 (IRF-1) was markedly enhanced in astrocytes treated with MDP. The potentiating effect of MDP on IFN-gamma-induced NO production in astrocytes was completely blocked with protein tyrosine kinase (PTK) inhibitor genistein or mitogen activated protein kinase (MAPK) inhibitor PD98059. In contrast, protein kinase C (PKC) inhibitor calphostin C did not affect ability of MDP to augment IFN-gamma-triggered astrocyte NO synthesis. These results suggest that MDP synergizes with IFN-gamma in the induction of iNOS gene in astrocytes through mechanisms involving PTK and MAPK, but not PKC activation. Finally, MDP also augmented NO production and iNOS mRNA expression in astrocytes treated with IL-1beta.
journal_name
Brain Resjournal_title
Brain researchauthors
Trajkovic V,Samardzic T,Stosic-Grujicic S,Ramic Z,Mostarica Stojkovic Mdoi
10.1016/s0006-8993(00)02920-6subject
Has Abstractpub_date
2000-11-10 00:00:00pages
157-63issue
1eissn
0006-8993issn
1872-6240pii
S0006899300029206journal_volume
883pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The endocrine disruptor Bisphenol-A (BPA) has been shown to modulate estrogenic, androgenic, and anti-androgenic effects. The effects of BPA exposure during early organizational periods of development have been well documented. The current study focuses on the effects of short term, low-dose BPA exposure on anxiety, s...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2013.07.018
更新日期：2013-09-05 00:00:00
abstract：:Reward anticipation is associated with activity in the dopaminergic midbrain as well as the ventral striatum, amygdala, and medial prefrontal cortex. Dopaminergic neuromodulation declines with age, suggesting that incentive processing should also undergo age-related change. However, the literature is mixed, perhaps re...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2014.09.063
更新日期：2015-07-01 00:00:00
abstract：:Individual neurons were recorded extracellularly in the nucleus accumbens (NAcc) of rats during cocaine self-administration sessions. NAcc neurons exhibited a variety of phasic changes in firing rate within the few seconds before and/or after cocaine self-infusion. Analysis of the topographical distribution of the pha...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00752-x
更新日期：1997-09-05 00:00:00
abstract：:We examined the effect of prostaglandin (PG)E2 on the sleep-wake activity and on body temperature by microinjecting PGE2 into the preoptic area of rats that had been chronically implanted with guide cannulae and electrodes for the recordings of electroencephalogram and electromyogram. PGE2 at doses of 2.5 X 10(-13), 2...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90935-3
更新日期：1988-03-22 00:00:00
abstract：:Sex hormone binding globulin (SHBG) is found in the brain and acts directly on plasma membrane-associated receptors in the prostate gland. Infusing SHBG into the medial preoptic area or medial basal hypothalamus of female rats increases their female sexual receptivity. SHBG, SHBG plus estradiol (SHBG-E), and SHBG-E pl...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02569-5
更新日期：2000-08-18 00:00:00
abstract：:Administration of methamphetamine (15 mg/kg) to adrenalectomized rats significantly decreased hippocampal type I and II corticosteroid receptors as well as type II receptors in the striatum. Type II receptors in the frontal cortex and hypothalamus were unaffected by methamphetamine administration. Pretreatment with MK...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91362-k
更新日期：1990-11-19 00:00:00
abstract：:The blink reflex consists of an early, pontine R1-component and a late, medullary R2-component. R1 and R2 can be evoked by innocuous stimuli, but only the R2 also by painful heat, suggesting that the R2 is mediated by wide dynamic range neurons (WDR) of the spinal trigeminal nucleus. Remote noxious stimuli suppress th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00646-5
更新日期：1998-08-24 00:00:00
abstract：INTRODUCTION:Ischemic postconditioning (IpostC) has been described in both heart and brain. The first aim of this study was to evaluate the effects of IpostC on brain infarct size and neurological function in the middle cerebral artery occlusion (MCAO) model. The second aim was to determine the involvement of the mitoc...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.12.054
更新日期：2011-02-23 00:00:00
abstract：:Reduced neuronal plasticity in the striatum, hippocampus, and neocortex is a common feature of transgenic mouse models of Huntington's disease (HD). Doublecortin (DCX) and polysialylated neural cell adhesion molecule (PSA-NCAM) are associated with structural plasticity in the adult mammalian brain, are markers of newl...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.03.018
更新日期：2007-06-02 00:00:00
abstract：:We determined whether pentobarbital anesthetic is required to culture postmortem adult rat neurons. Pentobarbital treatment resulted in two-fold increases in neuron survival in culture after 2 and 4 h postmortem compared to non-anesthetic controls, but was not as effective as simple postmortem treatment on ice and the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.03.006
更新日期：2004-05-29 00:00:00
abstract：:We examined the involvement of tumor necrosis factor (TNF)-alpha on glutamate-induced cytotoxicity in a differentiated neuronal cell line. In this study, we used nerve growth factor (NGF)-differentiated PC12h cells. Glutamate cytotoxicity was assessed using the MTS and TUNEL assays. To detect TNF-alpha levels in cultu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.09.006
更新日期：2006-11-29 00:00:00
abstract：:The precursor of the non-Abeta-component of Alzheimer's disease (AD) amyloid (NACP, alpha-synuclein) aggregates into insoluble filaments of Lewy bodies (LBs) in Parkinson's disease (PD) and dementia with LBs (DLB). The microtubule-associated protein tau is an integral component of filaments of neurofibrillary tangles ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01848-x
更新日期：1999-10-02 00:00:00
abstract：:In humans, tendon vibration evokes illusory sensations of movement that are usually associated with an excitatory tonic response in muscles antagonistic to those vibrated (antagonist vibratory response, AVR), i.e., in the muscle groups normally contracted if the illusory movement had been performed. The aim of the pre...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02604-4
更新日期：2000-10-27 00:00:00
abstract：:Estrogen has previously been shown to significantly change sympathetic and parasympathetic system output via an action within the central nuclei responsible for regulating autonomic tone. These estrogen-induced changes were observed within 30 min of systemic administration and could be blocked by the direct microinjec...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)03387-0
更新日期：2003-11-14 00:00:00
abstract：:Recent reports have suggested that the excitatory amino acid L-glutamate is a neurotransmitter released by baroreceptor afferent nerves at their termination in the nucleus tractus solitarii (NTS). In this study we have examined the effect on arterial pressure, heart rate and baroreflex activity of the glutamate antago...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90198-0
更新日期：1981-07-27 00:00:00
abstract：:Amino acid uptake by slices of brain is greatly diminished by incubation of the slices in glucose-free media. Uptake can be restored by the addition of a number of substrates, e.g., lactate, citrate, or oxaloacetate. The mixture of succinate, malate, and pyruvate (SMP, 20, 5, and mM) restored amino acid uptake better ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(77)90524-8
更新日期：1977-08-12 00:00:00
abstract：:Neural activity was recorded from 218 sites in the striatum (caudate-putamen and nucleus accumbens) of rats trained on a lever-release version of the conditioned avoidance response (CAR) task, in which an auditory signal elicits a short-latency, forelimb withdrawal. > 80% of these recording sites showed task-related a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90194-r
更新日期：1993-07-09 00:00:00
abstract：:Female rats were subjected to unilateral left or right, bilateral, or sham ablation of the cerebral cortex. Eight weeks after surgery, the rats were sacrificed by decapitation and spleen cells were grown in culture for 96 h in the presence or absence of the T-cell mitogen concanavalin A or the B-cell mitogen lipopolys...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90042-5
更新日期：1989-03-27 00:00:00
abstract：:Progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta is the main histopathological characteristic of Parkinson's disease. We studied the electrophysiological characteristics of the spontaneous activity of substantia nigra pars compacta dopaminergic neurons in rats with a partial, unil...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.02.052
更新日期：2006-04-21 00:00:00
abstract：:The amygdala plays an important role in the regulation of motivational states, especially those associated with addiction. The amygdala also expresses high levels of brain-derived neurotrophic factor (BDNF), an activity-dependent neurotrophin that can influence the reinforcing and locomotor activating properties of ps...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03160-8
更新日期：2002-09-13 00:00:00
abstract：:The pyrithiamine-treated rats produced by our method histopathologically exhibited the edematous lesion not only around the third ventricle and at the tegmentum of pons, as expected, but also in the cerebral cortex. These animals were, therefore, used in a biochemical study of the cerebral cortex, in particular, gluco...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91399-6
更新日期：1988-02-16 00:00:00
abstract：:Brains of reptiles, Caiman crocodilus, were processed by standard immunocytochemical methodology using a polyclonal antibody to gamma-aminobutyric acid (GABA) raised in rabbit and a polyclonal antibody to glutamic acid decarboxylase (GAD) raised in sheep. No GABA(+) or GAD(+) cells or puncta were observed over any tha...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90068-6
更新日期：1988-08-02 00:00:00
abstract：:A new compound, 3-((+/-)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP), has been evaluated as an excitatory amino acid receptor antagonist using electrophysiological assays and radioligand binding. In autoradiographic preparations, CPP reduces L-[3H]glutamate binding in regions of the hippocampus rich in N-m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90128-9
更新日期：1986-09-10 00:00:00
abstract：:The hippocampal formation of the rat contains two types of membrane-bound cholinergic binding sites, as revealed by specific binding of [3H]quinuclidinyl benzilate (QNB) or of a-[125I]bungarotoxin (a-Btx). The sites differ in pharmacological profile, sensitivity to detergents and ontogenesis. The major binding site (a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90211-7
更新日期：1979-04-27 00:00:00
abstract：:Incubation of rat striatal synaptosomes with the adenosine receptor agonist 2-chloroadenosine (2-CADO) produced a concentration-dependent increase of dopamine (DA) synthesis (about 50% of control value). The effect was not additive with the stimulation produced by either 10 microM forskolin or 2 mM dibutyryl cyclic AM...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90232-6
更新日期：1988-07-26 00:00:00
abstract：:In freely-moving rats treated with a combination of reserpine (10 mg/kg, i.p.) and scopolamine (5 mg/kg, i.p.), neocortical low voltage fast activity (LVFA) associated with continuous multiunit activity (MUA) was abolished and replaced by 2-6 Hz large irregular slow activity (LISA) above 1.5 mV associated with a burst...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00396-4
更新日期：1996-07-29 00:00:00
abstract：:The presence of calretinin and calbindin immunoreactivity is studied in the retina of larval and adult lamprey and their respective distributions are compared. Calretinin distribution is also studied in the retina of transforming stages. Western blot analysis in brain extracts showed a 29-kDa band with both polyclonal...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.11.014
更新日期：2006-01-12 00:00:00
abstract：:Phospholipid extracts from 48 intracranial tumors were analyzed using 31P NMR. Phospholipids commonly identified in the tumor spectra included phosphatidylglycerol (PG), phosphatidic acid (PA), diphosphatidylglycerol (DPG), uncharacterized phospholipid (U), ethanolamine plasmalogen (EPLAS), phosphatidylethanolamine (P...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91041-3
更新日期：1994-06-27 00:00:00
abstract：:Bradykinin is known to rapidly elevate intracellular calcium leading to secretion of neurotransmitters and short term activation of tyrosine hydroxylase (TH). In this study we examined the effect of bradykinin on mRNA levels of two catecholamine biosynthetic enzymes. Treatment of PC12 cells with 1 microM bradykinin fo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91477-a
更新日期：1993-04-16 00:00:00
abstract：:We have developed a serum-free chemically defined medium which allows the obtainment of a primary culture highly enriched in ciliated ependymal cells. Serum was never used. Mechanically dissociated neonatal rat brain hemisphere cells were seeded on a fibronectin substratum. Culture medium was minimum Eagle's medium un...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(86)80228-1
更新日期：1986-03-01 00:00:00
