Abstract:
:The hippocampal formation of the rat contains two types of membrane-bound cholinergic binding sites, as revealed by specific binding of [3H]quinuclidinyl benzilate (QNB) or of a-[125I]bungarotoxin (a-Btx). The sites differ in pharmacological profile, sensitivity to detergents and ontogenesis. The major binding site (about 17 pmol per adult hippocampus) is of a muscarinic nature, and binds [3H]QNB with an on-rate of 2 x 10(6) M-1 sec-1 and an apparent KD of 0.4 nM. This binding is displaced by low concentrations of muscarinic ligands but not of nicotinic ligands. The earliest increase in binding level is detected at about day 4 postnatal and a sharp increase in total binding takes place between days 10 and 15. Total binding continues to increase gradually about 3-fold until an age of about 7 weeks, at a rate resembling that of acetylcholinesterase. a-Btx-binding sites (about 0.6 pmol per adult hippocampus) display a nicotinic profile with an on-rate constant for a-[125I]Btx of 6 x 10(4) M-1 sec-1 and an apparent KD of 2 nM. Ontogenesis of these sites clearly differs from that of muscarinic sites and acetylcholinesterase. Absolute binding reaches mature levels at an age of 12--14 days postnatal, and binding per tissue protein is higher during the first postnatal days than at maturity. It appears that the level of toxin-binding sites attains mature values before the major synaptogenetic events in the area are completed.
journal_name
Brain Resjournal_title
Brain researchauthors
Ben-Barak J,Dudai Ydoi
10.1016/0006-8993(79)90211-7subject
Has Abstractpub_date
1979-04-27 00:00:00pages
245-57issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(79)90211-7journal_volume
166pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Our previous work has demonstrated that 100-Hz electroacupuncture (EA) or 100-Hz transcutaneous electrical nerve stimulation (TENS) was very effective in ameliorating the morphine withdrawal syndrome in rats and humans. The mechanism was obscure. (1) Rats were made dependent on morphine by repeated morphine injections...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02069-7
更新日期:1999-12-18 00:00:00
abstract::The alpha7 nicotinic receptor agonist 3-[2,4-dimethoxybenzylidene]anabaseine (DMXB; GTS-21) was investigated for its ability to: (1) activate a variety of nicotinic receptor subtypes in Xenopus oocytes; (2) improve passive avoidance and spatial Morris water task performances in mecamylamine-sensitive manners in bilate...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00536-2
更新日期:1997-09-12 00:00:00
abstract::Here we describe a sustained outward potassium current (IK) in retinal horizontal cells (HCs). IK is unusually small over the range of membrane potentials normally experienced by these cells, which are chronically depolarized. We hypothesize that this unique IK will reduce the amount of neurotransmitter required to sh...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90204-o
更新日期:1990-09-24 00:00:00
abstract::Fast axoplasmic transport was examined in experimental nerve ligation and adriamycin-induced neuronal perikaryon lesions. Animals were sacrificed at precisely 3 and 4 h following the injection of [3H]leucine. In single ligated nerves, the 3 and 4 h down-flow rates, which include the time for neuronal processing of lab...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(79)90730-3
更新日期:1979-07-27 00:00:00
abstract::An involvement of serotonin (5-HT) 1A receptors in the etiology of psychiatric disorders has been suggested. Hypo-responsiveness of the 5-HT1A receptor is linked to anxiety and constitutive deletion of the 5-HT1A receptor produces anxiety-like behaviors in the mouse. Evidence that 5-HT1A receptor inactivation increase...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)02271-5
更新日期:2001-05-25 00:00:00
abstract::A mutation in the Vps33a gene causes Hermansky-Pudlak Syndrome (HPS)-like-symptoms in the buff (bf) mouse mutant. The encoded product, Vps33a, is a member of the Sec1 and Class C multi-protein complex that regulates vesicle trafficking to specialized lysosome-related organelles. As Sec1 signaling pathways have been im...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.02.035
更新日期:2009-04-17 00:00:00
abstract::We investigated the effect of Cerebrospinal Fluid (CSF) from sporadic Amyotrophic Lateral Sclerosis patients (SALS-CSF) on motor neuron-like cells to delineate the pathomechanism of SALS. Exposure of NSC-34 cells to SALS-CSF caused lower viability, reduction in differentiation and enhanced lactate dehydrogenase activi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.01.041
更新日期:2009-03-31 00:00:00
abstract::To evaluate the role of the amygdala in pain modulation and opioid-mediated antinociception, a recombinant, replication-defective herpes virus carrying the human preproenkephalin cDNA was injected bilaterally into the rat amygdala. Four days after gene delivery nociceptive behavior was assessed by the formalin test. R...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)00194-2
更新日期:1998-05-04 00:00:00
abstract::The trisomy-16 mouse is considered to be a model of human trisomy-21 (Down syndrome). We have examined the electrical membrane properties of cultured dorsal root ganglion (DRG) neurons from normal and trisomy-16 fetuses. Trisomy-16 neurons had significantly accelerated rates of action potential depolarization and repo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90678-1
更新日期:1989-04-17 00:00:00
abstract::The cortical afferents from individual laminae of the dorsal lateral geniculate nucleus (LGN) were studied using both light and electron microscope autoradiography. In area 17, the A geniculate laminae (A and A1) had two main bands of projection, one extending from the bottom of IVc to the deepest cells in layer III, ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(76)90002-0
更新日期:1976-08-20 00:00:00
abstract::Oral ENA713 (0.5, 1.5 and 4.5 mg/kg), an acetylcholinesterase inhibitor (AChEI), dose-dependently enhanced extracellular acetylcholine concentrations in the hippocampus of freely moving rats. This effect was paralleled by changes in both noradrenergic and dopaminergic transmission. In particular, ENA713 significantly ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02266-6
更新日期:2000-05-26 00:00:00
abstract::S-allyl cysteine (SAC), a sulfur containing amino acid derived from garlic, has been reported to have antioxidant, anti-cancer, antihepatotoxic and neurotrophic activity. This study was designed to examine the pre-treatment effects of SAC on cognitive deficits and oxidative damage in the hippocampus of intracerebroven...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.02.072
更新日期:2011-05-10 00:00:00
abstract::Calcium-binding proteins play a critical role in vertebrate sensory cells, and some of them have been detected in mechanosensory and chemosensory cells of bony and cartilaginous fishes. In this study immunohistochemistry and Western blot were used to investigate the occurrence and the distribution of S100 protein and ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.05.070
更新日期:2007-08-08 00:00:00
abstract::Using nerve growth factor (NGF), anti-NGF sera and dissociated neonatal mouse dorsal root ganglionic neurons we present a microculture assay methodology for (1) the titration of neurotrophic factor (NTF) activity in monolayer culture, (2) the titration of NTF antibodies which 'block' NTF biological activity, (3) the t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90408-x
更新日期:1981-12-28 00:00:00
abstract::We investigated the effect of losartan, a nonpeptide angiotensin II (Ang II)-type 1 (AT1) receptor antagonist, on the responses evoked by Ang II and L-glutamate (L-Glu) in the rostral ventrolateral medulla (RVLM). Adult spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats were anesthetized with halothane ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91344-7
更新日期:1994-12-05 00:00:00
abstract::Missense mutations of presenilin 1 (PS-1) and presenilin 2 (PS-2) genes cause the majority of early-onset familial forms of Alzheimer's disease (AD). We previously characterized the distribution of the PS-1 protein in the mouse brain by immunohistochemistry using an antibody directed against an epitope located in the ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00231-x
更新日期:1997-05-30 00:00:00
abstract::Blockade of gamma-aminobutyric acid (GABA) function in the posterior hypothalamus of rats elicits a pattern of physiological and behavioral arousal consisting of increases in heart rate, respiration and blood pressure as well as intense locomotor stimulation and a selective enhancement of avoidance responding. The pre...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)91173-e
更新日期:1990-03-26 00:00:00
abstract::The hypoalgesic effects of lesions in the rat spinal trigeminal complex at the levels of subnucleus interpolaris and caudal subnucleus oralis were investigated. Lesions of the trigeminal tract, nucleus, or adjacent reticular formation resulted in significant elevations in escape latencies to noxious thermal stimulatio...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)91338-7
更新日期:1986-09-24 00:00:00
abstract::Adaptation in aging may become impaired from abnormal expression of corticotropin-releasing factor (CRF) and altered CRF receptor function. In this study, we measured CRF mRNA levels in Fischer 344 rats at various ages. The brains of these rats were processed for in situ hybridization. Relative to 3-month-old rats, le...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)01365-1
更新日期:1999-03-20 00:00:00
abstract::Repeated administration of subconvulsant doses of lidocaine or cocaine results in the development of an increased susceptibility to seizures induced by the two drugs (pharmacological kindling). It has been hypothesized that the local anesthetic properties of cocaine are responsible for its convulsant and epileptogenic...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90628-9
更新日期:1991-10-25 00:00:00
abstract::The neural representation of facial expression within the human visual system is not well defined. Using an adaptation paradigm, we examined aftereffects on expression perception produced by various stimuli. Adapting to a face, which was used to create morphs between two expressions, substantially biased expression pe...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.09.104
更新日期:2007-01-05 00:00:00
abstract::Experimental traumatic brain injury (TBI) is known to produce an acute increase in cerebral glucose utilization, followed rapidly by a generalized cerebral metabolic depression. The current studies determined effects of single or multiple treatments with sodium pyruvate (SP; 1000mg/kg, i.p.) or ethyl pyruvate (EP; 40m...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2016.04.005
更新日期:2016-07-01 00:00:00
abstract::Previous work has demonstrated that 3 pharmacologically and neuroanatomically distinct analgesia systems can be sequentially activated by increasing numbers of transcutaneous tail-shock. To date, the categorization of the early (after 2 tail-shocks) and late (after 80-100 tail-shocks) analgesias as opiate-mediated has...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90310-6
更新日期:1992-06-05 00:00:00
abstract::The opiate antagonist, naloxone, produces dose-dependent biphasic changes in clinical pain. The mechanism of the analgesia produced by low dose naloxone is unknown. To study the analgesic effect of naloxone, we have used a programmable infusion pump, which eliminates placebo-induced endorphin-mediated analgesia, to ad...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)91653-7
更新日期:1986-02-19 00:00:00
abstract::(1) The levels of glycolytic intermediates in the brain tissue of 15-day-old homozygous and heterozygous Gunn rats were determined with or without novobiocin treatment in vivo, and the inhibitory effects of bilirubin on the glycolytic activity and the key glycolytic enzyme activities were investigated in vitro. (2) t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(76)90945-8
更新日期:1976-08-27 00:00:00
abstract::The morphological and physiological identification of the cells in the inner retina of Japanese dace was made by means of intracellular single cell recording and dye injection techniques. Our flat mounts and physiological classification revealed that 97 out of 102 sample cells were amacrine cells except for 3 transien...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)91140-6
更新日期:1986-05-07 00:00:00
abstract::Techniques to induce activity-dependent neuronal plasticity in vivo allow the underlying signaling pathways to be studied in their biological context. Here, we demonstrate activity-induced plasticity at neuromuscular synapses of Drosophila double mutant for comatose (an NSF mutant) and Kum (a SERCA mutant), and presen...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.02.061
更新日期:2010-04-22 00:00:00
abstract::Mammalian target of rapamycin (mTOR) controls protein translation and has an important role in the mechanism of pain hypersensitivity under persistent pain conditions. However, its expression and localization in pain-related regions of the nervous system is not completely understood. Here, we examined the expression a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.04.010
更新日期:2010-06-08 00:00:00
abstract::To determine whether the idiosyncratic distribution of transduction mechanisms for bitter tastants in rat taste receptor cells (TRCs) could be inferred from the neural activity they evoke, single neuron responses to ten bitter-tasting compounds were recorded from rat glossopharyngeal (n = 30) and chorda tympani (n = 2...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00131-5
更新日期:1997-05-09 00:00:00
abstract::We used triple-labeling immunohistochemistry in rat midbrain sections to identify dopaminergic neurons that contain either one or both of the calcium-binding proteins, calretinin (CR) and calbindin-D28k (CB). Midbrain dopaminergic neurons were immunohistochemically labeled for tyrosine hydroxylase (TH), CR, and CB. In...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01950-2
更新日期:1999-10-30 00:00:00