Abstract：
:Using nerve growth factor (NGF), anti-NGF sera and dissociated neonatal mouse dorsal root ganglionic neurons we present a microculture assay methodology for (1) the titration of neurotrophic factor (NTF) activity in monolayer culture, (2) the titration of NTF antibodies which 'block' NTF biological activity, (3) the titration of NTF antibodies that bind and remove (sequester) NTF from culture medium and (4) a large-scale, convenient, and rapid screening for NTF biological activity as well as for NTF 'blocking' or 'sequestering' antibodies. These quantitative and qualitative in vitro microimmunoassays should be applicable to any neuronotrophic factor or its antibody, even when the agent is only available in crude, unpurified form. Since the microculture systems permit the simultaneous screening of one thousand samples per day they should be useful for the detection and quantitation of monoclonal antibodies present in hybridoma-conditioned media.
journal_name
Brain Resjournal_title
Brain researchauthors
Manthorpe M,Skaper SD,Varon Sdoi
10.1016/0006-8993(81)90408-xsubject
Has Abstractpub_date
1981-12-28 00:00:00pages
295-306issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(81)90408-Xjournal_volume
230pub_type
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