Abstract：
:We have successfully constructed a mouse neuronal N1E-115 cell line stably expressing a fully functional human dopamine transporter (hDAT). Previous studies in our laboratory have produced several anti-idiotypic Ab2beta antibodies that mimic the configuration of the cocaine molecule at their antigen-combining site. In the present study, we observed that some anti-idiotypic Ab2beta antibodies inhibited dopamine uptake by the hDAT-transfected line. Each antibody showed a different level of inhibition (between 40% and 90%). These findings suggest that the internal images of these antibodies can be used as analog peptides that may compete with cocaine for its binding site on the transporter but not impair dopamine uptake as much as cocaine does.
journal_name
Brain Resjournal_title
Brain researchauthors
Ho M,Segre Mdoi
10.1016/s0006-8993(00)02491-4subject
Has Abstractpub_date
2000-07-28 00:00:00pages
231-5issue
1-2eissn
0006-8993issn
1872-6240pii
S0006-8993(00)02491-4journal_volume
872pub_type
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