Abstract:
:The effect of thyroid hormone (TH) deficiency on the expression of neurofilament (NF) proteins and their intracellular distribution has been examined in primary cultures of neurons from embryonic rat cerebra. Northern blot analysis showed that in TH-deficient (THdef) neurons, the expression of all three NF mRNAs (NF-L, NF-M and NF-H) are retarded by 50-70% at days 7, 15 and 25 of culture compared with their TH-supplemented (THsup) counterparts. Western blot analysis also showed a decline of 60-80% in the expression of all the NF-proteins at all time points. Immunofluorescent staining of neurons from THdef and THsup cultures at days 7, 15, and 25 with monoclonal anti NF-L antibody showed that with age, TH deficiency reduced the localization of NF-L in the cell body with a corresponding increase in the hillock and proximal axon region. The present neuronal culture system offers an excellent experimental model for studying the biochemical events responsible for the altered NF pathology in degenerating neurons.
journal_name
Neuroreportjournal_title
Neuroreportauthors
Rahaman SO,Ghosh S,Mandal SK,Sarkar PKdoi
10.1097/00001756-200008210-00022subject
Has Abstractpub_date
2000-08-21 00:00:00pages
2717-22issue
12eissn
0959-4965issn
1473-558Xjournal_volume
11pub_type
杂志文章相关文献
NEUROREPORT文献大全abstract::Scanning laser-Doppler flowmetry (SLDF) combines laser-Doppler flowmetry and laser scanning to provide images of cerebral blood flow (CBF) with high spatial and temporal resolution. We investigated the contribution of single vascular elements to the local increase of CBF accompanying increased neuronal activity in hal...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199911080-00002
更新日期:1999-11-08 00:00:00
abstract::Experimental animals and patients are immobilized to allow continuous monitoring of physiological parameters. Restraint stress affects brain neurotransmitter levels and induces expression of immediate early genes. Whether immobilization stress affects outcomes in newborn animals which have suffered a hypoxic-ischaemic...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199601310-00025
更新日期:1996-01-31 00:00:00
abstract:OBJECTIVE:The traditional Chinese medicine, Dendrobium nobile Lindl. polysaccharides (DNLP), reportedly has neuroprotective effects. However, its effects following ischemic stroke remain unclear. This study aimed to explore the role and mechanism of DNLP in experimental models of inflammation and apoptosis. METHODS:In...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000001562
更新日期:2020-12-14 00:00:00
abstract::We investigated the role of melatonin in the induction of gastric lesions induced by water immersion restraint stress or centrally administered thyrotropin-releasing hormone (TRH). Melatonin (0.1-1 ng) injected intracisternally (i.c) 30 min prior to stress dose-dependently inhibited the induction of gastric lesions by...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199707070-00041
更新日期:1997-07-07 00:00:00
abstract::Participation of the neuronal nicotinic receptor subunit alpha6 in a physiologically relevant receptor has yet to be demonstrated, but its high degree of expression in catecholaminergic nuclei has attracted considerable interest. To investigate the pattern of expression of the alpha6 protein, a subunit specific antibo...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199708180-00019
更新日期:1997-08-18 00:00:00
abstract::Thyroid hormone receptor beta2 (TRbeta2) controls the patterning of cone opsin photopigments that mediate colour vision. We raised an antiserum against TRbeta2 to study cone photoreceptor development by western blot and immunostaining analyses. TRbeta2-positive cells first appeared between embryonic day 10 (E10) and E...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e32832a2c63
更新日期:2009-04-22 00:00:00
abstract::The present study examined the sleep-wake cycle effects of microinjections of yohimbine, an adrenergic antagonist, and atropine, a cholinergic antagonist, into the cerebral ventricle of clomipramine-induced depressed rats. Yohimbine microinjection caused a significant reduction in the total duration and the number of ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200209160-00006
更新日期:2002-09-16 00:00:00
abstract::This study compared the visuospatial asymmetries in children with dyslexia and healthy children by using the manual line bisection task, and investigated the processing of spatial context with a 'local' cueing paradigm consisting of geometric symbols placed on the extremities of the lines. The performance between heal...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e328342d2df
更新日期:2011-01-26 00:00:00
abstract::To test the hypothesis that the pedunclopontine tegmental nucleus (PPTg) plays a role in anxiety, rats with electrolytic lesions of the PPTg were tested in two animal models of anxiety, the elevated plus maze and the social interaction tests. Histological analysis showed that lesions were concentrated on the caudal ha...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199806010-00020
更新日期:1998-06-01 00:00:00
abstract::Light physiologically regulates the levels of c-Fos in the suprachiasmatic nucleus (SCN), but the identity of most of the cells that express this DNA-binding protein is unknown. To determine whether SCN c-Fos expression is restricted to neurons, we performed light microscopic double-label immunohistochemistry on secti...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199409080-00012
更新日期:1994-09-08 00:00:00
abstract::To investigate the implications of duration of deafness in the rehabilitation of unilateral deafness utilizing cochlear implantation. From the ongoing prospective cochlear implantation in unilateral deafness study, we looked at five adults who received a cochlear implant for long-term unilateral deafness. Speech perce...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e3283642a93
更新日期:2013-09-11 00:00:00
abstract::Previous studies in adult rats have demonstrated that the neurotrophin, brain-derived neurotrophic factor (BDNF), is present in virtually all cells of the pituitary intermediate lobe. In the present study, we demonstrate that cells cultured from adult intermediate lobe pituitary (ILP) rapidly lose their BDNF immunorea...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199703240-00005
更新日期:1997-03-24 00:00:00
abstract::We investigated the effects of radio frequency electromagnetic fields on brain physiology. Twenty-four healthy young men were exposed for 30 min to pulse-modulated or continuous-wave radio frequency electromagnetic fields (900 MHz; peak specific absorption rate 1 W/kg), or sham exposed. During exposure, participants p...
journal_title:Neuroreport
pub_type: 临床试验,杂志文章
doi:10.1097/WNR.0b013e3280d9435e
更新日期:2007-05-28 00:00:00
abstract::We investigated the effects of chemical hypoxia on the central pattern generator controlling swimming in stage 42 Xenopus laevis larvae. We recorded motoneuron activity from ventral roots of immobilized tadpoles and evoked swim episodes by brief electrical stimulation of the tail skin. In the presence of the metabolic...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e32833e332d
更新日期:2010-10-06 00:00:00
abstract::There is abundant psychophysical evidence in humans suggesting the existence of parallel pathways subserving different aspects of vision. However, there is little direct proof of the neural structures underlying the two pathways. We present direct anatomical evidence that in the normal human optic tract fibres are seg...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199407000-00005
更新日期:1994-07-21 00:00:00
abstract::It is a matter of dispute whether or not recurrent epileptic seizures cause neuronal damage in the human brain. We approached this question by measuring the volumes of the amygdala, hippocampus and parahippocampal gyrus with magnetic resonance imaging in 15 controls, 13 unmedicated patients with newly diagnosed epilep...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199412300-00055
更新日期:1994-12-30 00:00:00
abstract::Reward-processing neurocircuitry has been delineated using verbal or visual processing and/or decision-making tasks. We examined more basic processes of listening to enjoyable music in healthy and depressed patients. The paradigm was passive, individualized, and brief. Sixteen depressed and 15 control individuals prov...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e32832f4da3
更新日期:2009-08-26 00:00:00
abstract::We examined the effect of stimulus interruption on dual component field EPSPs in the hippocampal CA1 region. Resuming test stimulation at 0.1 Hz after 10-60 min silent periods led to an increase of the response followed by a decline, involving AMPA and NMDA components to a similar extent. Similar changes were seen whe...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199904060-00005
更新日期:1999-04-06 00:00:00
abstract::Aminoglycoside ototoxicity is a well-documented process via several pathophysiological pathways. The protective role of cochlear dopamine, released from the lateral olivocochlear efferents, was implicated previously in case of ischemia or acoustic trauma, as it postsynaptically inhibits the effect of excessively relea...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200110290-00036
更新日期:2001-10-29 00:00:00
abstract::Palmitoylethanolamine (PEA) is a ligand at peroxisome proliferator-activated receptors-α (PPARα), a nuclear receptor that has anti-inflammatory effects. Herein, complete Freund's adjuvant (CFA)-induced inflammatory pain model in rats and in-vitro calcium imaging studies were used to evaluate the mechanisms that underl...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000001161
更新日期:2019-01-02 00:00:00
abstract::In this study, the brainstem-spinal cord preparation isolated from newborn rats, an established model for the study of the hypoxic respiratory depression (HRD), has been used. The comparison of Fos expression in ponto-medullary areas in these preparations placed either in normoxic or hypoxic conditions suggests that o...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200112210-00012
更新日期:2001-12-21 00:00:00
abstract::In previous studies, we demonstrated functional neuronal and dopaminergic differentiation of fetal mesencephalic neural precursor cells. The major factors for orienting their progeny towards a dopaminergic phenotype are forskolin and interleukin-1beta. Here, we investigated the effects of dexamethasone (10 microM) on ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/01.wnr.0000236862.08834.50
更新日期:2006-11-06 00:00:00
abstract::Prefrontal delay-period activity represents a neural mechanism for the active maintenance of information and needs to be controlled by some signal to appropriately operate working memory. To examine whether reward-delivery acts as this signal, the effects of delay-period activity in response to unexpected reward-deliv...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e32833776a3
更新日期:2010-03-31 00:00:00
abstract::Secondary brain injury imposes great effects on the prognosis of patients following traumatic brain injury (TBI). Accumulating evidence suggests that nuclear factor erythroid 2-related factor 2 (Nrf2) could play a neuroprotective role in experimental TBI models by regulating the expression of numerous antioxidant, ant...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000001205
更新日期:2019-03-20 00:00:00
abstract::The role of the right hemisphere in recovery of language function after left hemisphere stroke remains unclear. This prospective study aimed to determine the relationship between bilateral hemispheric changes and severity of aphasia in patients with chronic left hemisphere stroke. Twenty-three patients with left hemis...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000000929
更新日期:2018-01-03 00:00:00
abstract::Interleukin-2 and its receptors are present in hippocampal structures, particularly the pyramidal cell layer. In the present study, we examined the interaction between recombinant human interleukin-2 (rhIL-2) and the voltage-dependent calcium (Ca2+) current using the whole-cell patch-clamp technique. RhIL-2 depressed ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199305000-00030
更新日期:1993-05-01 00:00:00
abstract::In adults, neural networks for phonological processing distinct from those involved in acoustical processing are located in the left temporal lobe. We now report that by the age of 3 months, infants display phonological processing devices analogous to those found in adults. Within a stream of identical syllables, acou...
journal_title:Neuroreport
pub_type: 临床试验,杂志文章
doi:10.1097/00001756-199806010-00040
更新日期:1998-06-01 00:00:00
abstract::Where and how the cortical areas are activated when a person receives the result of hypothesis testing is still unclear. By using a category induction task, this study provided participants with various batteries and asked them to learn which kind of batteries were charged. After learning, they were required to give a...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e32831d7f6b
更新日期:2009-01-28 00:00:00
abstract::By using high-resolution, conventional, and neuromelanin-sensitive magnetic resonance imaging techniques, we reviewed the normal anatomy of the nuclei consisting of monoamine neurons such as dopaminergic, noradrenergic, and serotoninergic neurons and noted the changes in these nuclei that occur in some degenerative an...
journal_title:Neuroreport
pub_type: 杂志文章,评审
doi:10.1097/WNR.0b013e328315a637
更新日期:2008-11-19 00:00:00
abstract::Between 20 and 30% of AIDS patients have neurological symptoms characterized by motor impairment, memory loss and progressive dementia. Previous studies have implicated the HIV derived gp120, which produces behavioral deficits and electrophysiological alterations in rats. The goal of the present study was to describe ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200004270-00040
更新日期:2000-04-27 00:00:00